cdc25 is one of the MPM-2 antigens involved in the activation of maturation-promoting factor.

abstract：:Yeast that naturally exhaust the glucose from their environment differentiate into three distinct cell types distinguishable by flow cytometry. Among these is a quiescent (Q) population, which is so named because of its uniform but readily reversed G1 arrest, its fortified cell walls, heat tolerance, and longevity. Da...

journal_title：Molecular biology of the cell

authors： Li L,Miles S,Melville Z,Prasad A,Bradley G,Breeden LL

更新日期：2013-12-01 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00

abstract：:Cell migration involves the cooperative reorganization of the actin and microtubule cytoskeletons, as well as the turnover of cell-substrate adhesions, under the control of Rho family GTPases. RhoA is activated at the leading edge of motile cells by unknown mechanisms to control actin stress fiber assembly, contractil...

journal_title：Molecular biology of the cell

authors： Nalbant P,Chang YC,Birkenfeld J,Chang ZF,Bokoch GM

更新日期：2009-09-01 00:00:00

abstract：:The Golgi apparatus is a membrane-bound organelle that serves as the center for trafficking and processing of proteins and lipids. To perform these functions, the Golgi forms a multilayer stacked structure held by GRASP55 and GRASP65 trans-oligomers and perhaps their binding partners. Depletion of GRASP proteins disru...

journal_title：Molecular biology of the cell

authors： Ahat E,Xiang Y,Zhang X,Bekier ME 2nd,Wang Y

更新日期：2019-03-15 00:00:00

abstract：:The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area changes during fibroblast sp...

journal_title：Molecular biology of the cell

authors： Gauthier NC,Rossier OM,Mathur A,Hone JC,Sheetz MP

更新日期：2009-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many osmotically inducible genes are regulated by the Sko1p-Ssn6p-Tup1p complex. On osmotic shock, the MAP kinase Hog1p associates with this complex, phosphorylates Sko1p, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. We have found that phospholip...

journal_title：Molecular biology of the cell

authors： Guha N,Desai P,Vancura A

更新日期：2007-07-01 00:00:00

abstract：:Maturation-promoting factor, consisting of cdc2 protein kinase and a regulatory B-type cyclin, is a universal regulator of meiosis and mitosis in eukaryotes. In Xenopus, there are two subtypes of B-type cyclins, designated B1 and B2, both of which are phosphorylated. In this study, we have investigated the biological ...

journal_title：Molecular biology of the cell

authors： Li J,Meyer AN,Donoghue DJ

更新日期：1995-09-01 00:00:00

abstract：:Jak tyrosine kinases have a unique domain structure containing a kinase domain (JH1) adjacent to a catalytically inactive pseudokinase domain (JH2). JH2 is crucial for inhibition of basal Jak activity, but the mechanism of this regulation has remained elusive. We show that JH2 negatively regulated Jak2 in bacterial ce...

journal_title：Molecular biology of the cell

authors： Saharinen P,Vihinen M,Silvennoinen O

更新日期：2003-04-01 00:00:00

abstract：:Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)-anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin Va (MyoVa)-dependent secretory granule (SG) transport and secretion in...

journal_title：Molecular biology of the cell

authors： Brozzi F,Lajus S,Diraison F,Rajatileka S,Hayward K,Regazzi R,Molnár E,Váradi A

更新日期：2012-11-01 00:00:00

abstract：:The Notch signaling pathway plays essential roles in both animal development and human disease. Regulation of Notch receptor levels in membrane compartments has been shown to affect signaling in a variety of contexts. Here we used steady-state and pulse-labeling techniques to follow Notch receptors in sensory organ pr...

journal_title：Molecular biology of the cell

authors： Johnson SA,Zitserman D,Roegiers F

更新日期：2016-09-15 00:00:00

abstract：:The septins are a family of homologous proteins that were originally identified in Saccharomyces cerevisiae, where they are associated with the "neck filaments" and are involved in cytokinesis and other aspects of the organization of the cell surface. We report here the identification of Sep1, a Drosophila melanogaste...

journal_title：Molecular biology of the cell

authors： Fares H,Peifer M,Pringle JR

更新日期：1995-12-01 00:00:00

abstract：:SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosph...

journal_title：Molecular biology of the cell

authors： Sakurai C,Itakura M,Kinoshita D,Arai S,Hashimoto H,Wada I,Hatsuzawa K

更新日期：2018-07-15 00:00:00

abstract：:The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein heavy chain and may mediate the attachment of dynein to centrosomes and o...

journal_title：Molecular biology of the cell

authors： Mische S,He Y,Ma L,Li M,Serr M,Hays TS

更新日期：2008-11-01 00:00:00

abstract：:The TOR kinases are conserved negative regulators of autophagy in response to nutrient conditions, but the signaling mechanisms are poorly understood. Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila. We s...

journal_title：Molecular biology of the cell

authors： Chang YY,Neufeld TP

更新日期：2009-04-01 00:00:00

abstract：:NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss, its precise functi...

journal_title：Molecular biology of the cell

authors： Rajeevan A,Keshri R,Kapoor S,Kotak S

更新日期：2020-10-15 00:00:00

abstract：:Posttranslationally modified forms of tubulin accumulate in the subset of stabilized microtubules (MTs) in cells but are not themselves involved in generating MT stability. We showed previously that stabilized, detyrosinated (Glu) MTs function to localize vimentin intermediate filaments (IFs) in fibroblasts. To determ...

journal_title：Molecular biology of the cell

authors： Kreitzer G,Liao G,Gundersen GG

更新日期：1999-04-01 00:00:00

abstract：:Inactivation of YME1 in yeast causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment. T...

journal_title：Molecular biology of the cell

authors： Weber ER,Hanekamp T,Thorsness PE

更新日期：1996-02-01 00:00:00

abstract：:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes to...

journal_title：Molecular biology of the cell

authors： Saini SG,Liu C,Zhang P,Lee TH

更新日期：2014-12-01 00:00:00

abstract：:Immunolocalization studies in epithelial cells revealed myo6 was associated with peripherally located vesicles that contained the transferrin receptor. Pulse-chase experiments after transferrin uptake showed that these vesicles were newly uncoated endocytic vesicles and that myo6 was recruited to these vesicles immedi...

journal_title：Molecular biology of the cell

authors： Aschenbrenner L,Lee T,Hasson T

更新日期：2003-07-01 00:00:00

abstract：:Actin-based protrusions are important for signaling and migration during development and homeostasis. Defining how different tissues in vivo craft diverse protrusive behaviors using the same genomic toolkit of actin regulators is a current challenge. The actin elongation factors Diaphanous and Enabled both promote bar...

journal_title：Molecular biology of the cell

authors： Nowotarski SH,McKeon N,Moser RJ,Peifer M

更新日期：2014-10-15 00:00:00

abstract：:The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endo...

journal_title：Molecular biology of the cell

authors： Tabata K,Matsunaga K,Sakane A,Sasaki T,Noda T,Yoshimori T

更新日期：2010-12-01 00:00:00

abstract：:We applied a combination of laser microsurgery and quantitative polarization microscopy to study kinetochore-independent forces that act on chromosome arms during meiosis in crane fly spermatocytes. When chromosome arms located within one of the half-spindles during prometa- or metaphase were cut with the laser, the a...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R,Cole RW,Rieder CL

更新日期：2001-12-01 00:00:00

abstract：:We have shown that protein kinase A phosphorylation of t-SNAREs inhibits SNARE assembly and suppresses endo- and exocytosis in yeast. Herein, we show that protein kinase A phosphorylation of the Sso exocytic t-SNAREs promotes the binding of Vsm1, a potential SNARE regulator identified previously in our laboratory. Pho...

journal_title：Molecular biology of the cell

authors： Marash M,Gerst JE

更新日期：2003-08-01 00:00:00

abstract：:The split-ubiquitin technique was used to detect transient protein interactions in living cells. Nub, the N-terminal half of ubiquitin (Ub), was fused to Sec62p, a component of the protein translocation machinery in the endoplasmic reticulum of Saccharomyces cerevisiae. Cub, the C-terminal half of Ub, was fused to the...

journal_title：Molecular biology of the cell

authors： Dünnwald M,Varshavsky A,Johnsson N

更新日期：1999-02-01 00:00:00

abstract：:Notch and neurotrophins control neuronal shape, but it is not known whether their signaling pathways intersect. Here we report results from hippocampal neuronal cultures that are in support of this possibility. We found that low cell density or blockade of Notch signaling by a soluble Delta-Fc ligand decreased the mRN...

journal_title：Molecular biology of the cell

authors： Salama-Cohen P,Arévalo MA,Meier J,Grantyn R,Rodríguez-Tébar A

更新日期：2005-01-01 00:00:00

abstract：:Intracellular survival of Salmonella relies on the activity of proteins translocated into the host cell by type III secretion systems (T3SS). The protein kinase activity of the T3SS effector SteC is required for F-actin remodeling in host cells, although no SteC target has been identified so far. Here we show that exp...

journal_title：Molecular biology of the cell

authors： Fernandez-Piñar P,Alemán A,Sondek J,Dohlman HG,Molina M,Martín H

更新日期：2012-11-01 00:00:00

abstract：:Early endosomes in PC12 cells are an important site for the formation of synaptic-like microvesicles and constitutive recycling vesicles. By immunogold electron microscopy, the small GTPase rab4 was localized to early endosomes and numerous small vesicles in the cell periphery and Golgi area of PC12 cells. Overexpress...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Kelly RB,Geuze HJ,Klumperman J,van der Sluijs P

更新日期：2001-11-01 00:00:00

abstract：:The secretory and autophagy pathways can be thought of as the biosynthetic (i.e., anabolic) and degradative (i.e., catabolic) branches of the endomembrane system. In analogy to anabolic and catabolic pathways in metabolism, there is mounting evidence that the secretory and autophagy pathways are intimately linked and ...

journal_title：Molecular biology of the cell

authors： Farhan H,Kundu M,Ferro-Novick S

更新日期：2017-05-01 00:00:00

abstract：:High-grade gliomas are especially difficult tumors to treat due to their invasive behavior. This has led to extensive research focusing on arresting glioma cell migration. Cell migration involves the sensing of a migratory cue, followed by polarization in the direction of the cue, and reorganization of the actin cytos...

journal_title：Molecular biology of the cell

authors： Mair DB,Ames HM,Li R

更新日期：2018-10-15 00:00:00

abstract：:The mitotic exit network (MEN) is an essential GTPase signaling pathway that triggers exit from mitosis in budding yeast. We show here that during meiosis, the MEN is dispensable for exit from meiosis I but contributes to the timely exit from meiosis II. Consistent with a role for the MEN during meiosis II, we find th...

journal_title：Molecular biology of the cell

authors： Attner MA,Amon A

更新日期：2012-08-01 00:00:00