Assembly of the mitochondrial protein import channel: role of Tom5 in two-stage interaction of Tom40 with the SAM complex.

abstract：:Early endosomes in PC12 cells are an important site for the formation of synaptic-like microvesicles and constitutive recycling vesicles. By immunogold electron microscopy, the small GTPase rab4 was localized to early endosomes and numerous small vesicles in the cell periphery and Golgi area of PC12 cells. Overexpress...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Kelly RB,Geuze HJ,Klumperman J,van der Sluijs P

更新日期：2001-11-01 00:00:00

abstract：:SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci-infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP-transfected HeLa cells indicate that SINC targets the inner nuclear membrane. SI...

journal_title：Molecular biology of the cell

authors： Mojica SA,Hovis KM,Frieman MB,Tran B,Hsia RC,Ravel J,Jenkins-Houk C,Wilson KL,Bavoil PM

更新日期：2015-05-15 00:00:00

abstract：:The Src homology (SH2 and SH3) domains of v-Src are required for transformation of Rat-2 cells and for wild-type (morphr) transformation of chicken embryo fibroblasts (CEFs). We report herein that the N-terminal domains of v-Src, when expressed in trans, cannot complement the transformation defect of a deletion mutant...

journal_title：Molecular biology of the cell

authors： Tian M,Martin GS

更新日期：1997-07-01 00:00:00

abstract：:Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. Claspin associates with replication forks upon origin unwinding. We show that Claspin contains a replication fork-interacting domain (RFID, residues 265-605) that associates with Cdc45, DNA pol...

journal_title：Molecular biology of the cell

authors： Lee J,Gold DA,Shevchenko A,Shevchenko A,Dunphy WG

更新日期：2005-11-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. One feature of VEGF is the existence of multiple isoforms arising from alternative exon splicing. Our initial biochemical and biological studies indicated that such isoforms are uniquely suited to gen...

journal_title：Molecular biology of the cell

authors： Ferrara N

更新日期：2010-03-01 00:00:00

abstract：:The ARF tumor suppressor is a product of the INK4a/ARF locus, which is frequently mutated in human cancer. The expression of ARF is up-regulated in response to certain types of DNA damage, oncogene activation, and interferon stimuli. Through interaction with the p53 negative regulator MDM2, ARF controls a well-describ...

journal_title：Molecular biology of the cell

authors： Li Z,Hou J,Sun L,Wen T,Wang L,Zhao X,Xie Q,Zhang SQ

更新日期：2012-12-01 00:00:00

abstract：:During oocyte maturation and early development, mRNAs receive poly(A) in the cytoplasm at distinct times relative to one another and to the cell cycle. These cytoplasmic polyadenylation reactions do not occur during oogenesis, but begin during oocyte maturation and continue throughout early development. In this report...

journal_title：Molecular biology of the cell

authors： Ballantyne S,Daniel DL Jr,Wickens M

更新日期：1997-08-01 00:00:00

abstract：:Cadherin-based cell-cell contacts are prominent sites for phosphotyrosine signaling, being enriched in tyrosine-phosphorylated proteins and tyrosine kinases and phosphatases. The functional interplay between cadherin adhesion and tyrosine kinase signaling, however, is complex and incompletely understood. In this repor...

journal_title：Molecular biology of the cell

authors： McLachlan RW,Kraemer A,Helwani FM,Kovacs EM,Yap AS

更新日期：2007-08-01 00:00:00

abstract：:In vertebrate cells, the nucleoporin Nup358/RanBP2 is a major component of the filaments that emanate from the nuclear pore complex into the cytoplasm. Nup358 forms a complex with SUMOylated RanGAP1, the GTPase activating protein for Ran. RanGAP1 plays a pivotal role in the establishment of a RanGTP gradient across th...

journal_title：Molecular biology of the cell

authors： Hutten S,Flotho A,Melchior F,Kehlenbach RH

更新日期：2008-05-01 00:00:00

abstract：:MARK2 regulates the establishment of polarity in Madin-Darby canine kidney (MDCK) cells in part through phosphorylation of serine 227 of Rab11-FIP2. We identified Eps15 as an interacting partner of phospho-S227-Rab11-FIP2 (pS227-FIP2). During recovery from low calcium, Eps15 localized to the lateral membrane before pS...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Manning EH,Mitchell KM,Caldwell CM,Goldenring JR

更新日期：2017-04-15 00:00:00

abstract：:The complex cytopathology of mitochondrial diseases is usually attributed to insufficient ATP. AMP-activated protein kinase (AMPK) is a highly sensitive cellular energy sensor that is stimulated by ATP-depleting stresses. By antisense-inhibiting chaperonin 60 expression, we produced mitochondrially diseased strains wi...

journal_title：Molecular biology of the cell

authors： Bokko PB,Francione L,Bandala-Sanchez E,Ahmed AU,Annesley SJ,Huang X,Khurana T,Kimmel AR,Fisher PR

更新日期：2007-05-01 00:00:00

abstract：:WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To...

journal_title：Molecular biology of the cell

authors： Patel-King RS,King SM

更新日期：2016-04-15 00:00:00

abstract：:Rods and rings (RRs) are large linear- or circular-shaped structures typically described as polymers of IMPDH (inosine monophosphate dehydrogenase). They have been observed across a wide variety of cell types and species and can be induced to form by inhibitors of IMPDH. RRs are thought to play a role in the regulatio...

journal_title：Molecular biology of the cell

authors： Schiavon CR,Griffin ME,Pirozzi M,Parashuraman R,Zhou W,Jinnah HA,Reines D,Kahn RA

更新日期：2018-09-15 00:00:00

abstract：:The organization of the endoplasmic reticulum (ER) in the cortex of Xenopus oocytes was investigated during maturation and activation using a green fluorescent protein chimera, immunofluorescence, and electron microscopy. Dense clusters of ER developed on the vegetal side (the side opposite the meiotic spindle) during...

journal_title：Molecular biology of the cell

authors： Terasaki M,Runft LL,Hand AR

更新日期：2001-04-01 00:00:00

abstract：:The late Golgi of the yeast Saccharomyces cerevisiae receives membrane traffic from the secretory pathway as well as retrograde traffic from post-Golgi compartments, but the machinery that regulates these vesicle-docking and fusion events has not been characterized. We have identified three components of a novel prote...

journal_title：Molecular biology of the cell

authors： Conibear E,Stevens TH

更新日期：2000-01-01 00:00:00

abstract：:Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

journal_title：Molecular biology of the cell

authors： Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

更新日期：2019-05-01 00:00:00

abstract：:Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the p...

journal_title：Molecular biology of the cell

authors： Assaad FF,Qiu JL,Youngs H,Ehrhardt D,Zimmerli L,Kalde M,Wanner G,Peck SC,Edwards H,Ramonell K,Somerville CR,Thordal-Christensen H

更新日期：2004-11-01 00:00:00

abstract：:Immunolocalization studies in epithelial cells revealed myo6 was associated with peripherally located vesicles that contained the transferrin receptor. Pulse-chase experiments after transferrin uptake showed that these vesicles were newly uncoated endocytic vesicles and that myo6 was recruited to these vesicles immedi...

journal_title：Molecular biology of the cell

authors： Aschenbrenner L,Lee T,Hasson T

更新日期：2003-07-01 00:00:00

abstract：:Candida albicans must undergo a switch from white to opaque to mate. Opaque cells then release mating type-specific pheromones that induce mating responses in opaque cells. Uniquely in C. albicans, the same pheromones induce mating-incompetent white cells to become cohesive, form an adhesive basal layer of cells on a ...

journal_title：Molecular biology of the cell

authors： Yi S,Sahni N,Daniels KJ,Pujol C,Srikantha T,Soll DR

更新日期：2008-03-01 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:Studies on physiological modulation of intercellular communication mediated by protein kinases are often complicated by the fact that cells express multiple gap junction proteins (connexins; Cx). Changes in cell coupling can be masked by simultaneous opposite regulation of the gap junction channel types expressed. We ...

journal_title：Molecular biology of the cell

authors： Kwak BR,Hermans MM,De Jonge HR,Lohmann SM,Jongsma HJ,Chanson M

更新日期：1995-12-01 00:00:00

abstract：:On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis to cyclic AMP (cAMP) and relay of the cAMP signal by the activation of adenylyl cyclase (ACA), and it has been shown prev...

journal_title：Molecular biology of the cell

authors： Bolourani P,Spiegelman GB,Weeks G

更新日期：2006-10-01 00:00:00

abstract：:Proper functioning of the protein-folding quality control network depends on the network's ability to discern diverse structural perturbations to the native states of its protein substrates. Despite the centrality of the detection of misfolded states to cell home-ostasis, very little is known about the exact sequence ...

journal_title：Molecular biology of the cell

authors： Furth N,Gertman O,Shiber A,Alfassy OS,Cohen I,Rosenberg MM,Doron NK,Friedler A,Ravid T

更新日期：2011-12-01 00:00:00

abstract：:The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma membrane (PM). In this study, we re...

journal_title：Molecular biology of the cell

authors： Liu J,Zuo X,Yue P,Guo W

更新日期：2007-11-01 00:00:00

abstract：:Previous research has found that a gamma-tubulin mutation in Schizosaccharomyces pombe is synthetically lethal with a deletion of the C-terminal motor domain kinesin-like protein gene pkl1, but the lethality of the double mutant prevents a phenotypic analysis of the synthetic interaction. We have investigated interact...

journal_title：Molecular biology of the cell

authors： Prigozhina NL,Walker RA,Oakley CE,Oakley BR

更新日期：2001-10-01 00:00:00

abstract：:When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins a...

journal_title：Molecular biology of the cell

authors： González-Novo A,Correa-Bordes J,Labrador L,Sánchez M,Vázquez de Aldana CR,Jiménez J

更新日期：2008-04-01 00:00:00

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:Hsk1, Saccharomyces cerevisiae Cdc7-related kinase in Shizosaccharomyces pombe, is required for G1/S transition and its kinase activity is controlled by the regulatory subunit Dfp1/Him1. Analyses of a newly isolated temperature-sensitive mutant, hsk1-89, reveal that Hsk1 plays crucial roles in DNA replication checkpoi...

journal_title：Molecular biology of the cell

authors： Takeda T,Ogino K,Tatebayashi K,Ikeda H,Arai Ki,Masai H

更新日期：2001-05-01 00:00:00

abstract：:The neonatal receptor for immunoglobulin G (IgG; FcRn) prevents IgG degradation by efficiently sorting IgG into recycling endosomes and away from lysosomes. When bound to IgG-opsonized antigen complexes, however, FcRn traffics cargo into lysosomes, where antigen processing can occur. Here we address the mechanism of s...

journal_title：Molecular biology of the cell

authors： Weflen AW,Baier N,Tang QJ,Van den Hof M,Blumberg RS,Lencer WI,Massol RH

更新日期：2013-08-01 00:00:00

abstract：:The reovirus fusion-associated small transmembrane (FAST) proteins comprise a unique family of viral membrane fusion proteins dedicated to inducing cell-cell fusion. We recently reported that a polybasic motif (PBM) in the cytosolic tail of reptilian reovirus p14 FAST protein functions as a novel tribasic Golgi export...

journal_title：Molecular biology of the cell

authors： Parmar HB,Duncan R

更新日期：2016-04-15 00:00:00