Sep7 is essential to modify septin ring dynamics and inhibit cell separation during Candida albicans hyphal growth.

abstract：:To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...

journal_title：Molecular biology of the cell

authors： Drubin DG,Jones HD,Wertman KF

更新日期：1993-12-01 00:00:00

abstract：:Dysregulated hepatic cholesterol homeostasis with free cholesterol accumulation in the liver is relevant to the pathogenesis of nonalcoholic steatohepatitis, contributing to the chronicity of liver toxicity. Here we examined the effect of free cholesterol accumulation on the morphology and biochemical properties of li...

journal_title：Molecular biology of the cell

authors： Makino A,Hullin-Matsuda F,Murate M,Abe M,Tomishige N,Fukuda M,Yamashita S,Fujimoto T,Vidal H,Lagarde M,Delton I,Kobayashi T

更新日期：2016-11-01 00:00:00

abstract：:Damaged DNA-binding protein 2 (DDB2), a nuclear protein, participates in both nucleotide excision repair and mRNA transcription. The transcriptional regulatory function of DDB2 is significant in colon cancer, as it regulates metastasis. To characterize the mechanism by which DDB2 participates in transcription, we inve...

journal_title：Molecular biology of the cell

authors： Fantini D,Huang S,Asara JM,Bagchi S,Raychaudhuri P

更新日期：2017-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many osmotically inducible genes are regulated by the Sko1p-Ssn6p-Tup1p complex. On osmotic shock, the MAP kinase Hog1p associates with this complex, phosphorylates Sko1p, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. We have found that phospholip...

journal_title：Molecular biology of the cell

authors： Guha N,Desai P,Vancura A

更新日期：2007-07-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:In mammals, repression of translation during stress is associated with the assembly of stress granules in the cytoplasm, which contain a fraction of arrested mRNA and have been proposed to play a role in their storage. Because physical contacts are seen with GW bodies, which contain the mRNA degradation machinery, str...

journal_title：Molecular biology of the cell

authors： Mollet S,Cougot N,Wilczynska A,Dautry F,Kress M,Bertrand E,Weil D

更新日期：2008-10-01 00:00:00

abstract：:The potential to migrate is one of the most fundamental functions for various epithelial, mesenchymal, and immune cells. Image analysis of motile cell populations, both primary and cultured, typically reveals an intercellular variability in migration speeds. However, cell migration chromatography, the sorting of large...

journal_title：Molecular biology of the cell

authors： Arora A,Niño JLG,Myaing MZ,Chia S,Arasi B,Ravasio A,Huang RY,Dasgupta R,Biro M,Viasnoff V

更新日期：2020-12-01 00:00:00

abstract：:Septins are members of a highly conserved family of filamentous proteins that are required in many organisms for the completion of cytokinesis. In addition, septins have been implicated in a number of important cellular processes and have been suggested to have roles in regulating membrane traffic. Given the proposed ...

journal_title：Molecular biology of the cell

authors： Huang YW,Yan M,Collins RF,Diciccio JE,Grinstein S,Trimble WS

更新日期：2008-04-01 00:00:00

abstract：:NSF and p97 are related AAA proteins implicated in membrane trafficking and organelle biogenesis. p97 is also involved in pathways that lead to ubiquitin-dependent proteolysis, including ER-associated degradation (ERAD). In this study, we have used dominant interfering ATP-hydrolysis deficient mutants (NSF(E329Q) and ...

journal_title：Molecular biology of the cell

authors： Dalal S,Rosser MF,Cyr DM,Hanson PI

更新日期：2004-02-01 00:00:00

abstract：:During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understo...

journal_title：Molecular biology of the cell

authors： Xie S,Mason FM,Martin AC

更新日期：2016-11-07 00:00:00

abstract：:Epithelial tissues are protective barriers that display a remarkable ability to repair wounds. Wound repair is often associated with an accumulation of actin and nonmuscle myosin II around the wound, forming a purse string. The role of actomyosin networks in generating mechanical force during wound repair is not well ...

journal_title：Molecular biology of the cell

authors： Fernandez-Gonzalez R,Zallen JA

更新日期：2013-10-01 00:00:00

abstract：:Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

journal_title：Molecular biology of the cell

authors： Bendezú FO,Martin SG

更新日期：2011-01-01 00:00:00

abstract：:Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrate that phosphodiesterase type 3A (PDE3A) physically and functionally interacts with cystic fibrosis transmembrane conductance regula...

journal_title：Molecular biology of the cell

authors： Penmatsa H,Zhang W,Yarlagadda S,Li C,Conoley VG,Yue J,Bahouth SW,Buddington RK,Zhang G,Nelson DJ,Sonecha MD,Manganiello V,Wine JJ,Naren AP

更新日期：2010-03-15 00:00:00

abstract：:Pro-alpha-factor (pro-alphaf) is posttranslationally modified in the yeast Golgi complex by the addition of alpha1,6-, alpha1,2-, and alpha1,3-linked mannose to N-linked oligosaccharides and by a Kex2p-initiated proteolytic processing event. Previous work has indicated that the alpha1,6- and alpha1,3-mannosylation and...

journal_title：Molecular biology of the cell

authors： Brigance WT,Barlowe C,Graham TR

更新日期：2000-01-01 00:00:00

abstract：:Myosin Va is associated with discrete vesicle populations in a number of cell types, but little is known of the function of myosin Vb. Yeast two-hybrid screening of a rabbit parietal cell cDNA library with dominant active Rab11a (Rab11aS20V) identified myosin Vb as an interacting protein for Rab11a, a marker for plasm...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Kumar R,Hales CM,Navarre J,Bhartur SG,Burnette JO,Provance DW Jr,Mercer JA,Bähler M,Goldenring JR

更新日期：2001-06-01 00:00:00

abstract：:GPI-linked hemagglutinin (GPI-HA) of influenza virus was thought to induce hemifusion without pore formation. Cells expressing either HA or GPI-HA were bound to red blood cells, and their fusion was compared by patch-clamp capacitance measurements and fluorescence microscopy. It is now shown that under more optimal fu...

journal_title：Molecular biology of the cell

authors： Markosyan RM,Cohen FS,Melikyan GB

更新日期：2000-04-01 00:00:00

abstract：:The majority of Rab proteins are posttranslationally modified with two geranylgeranyl lipid moieties that enable their stable association with membranes. In this study, we present evidence to demonstrate that there is a specific lipid requirement for Rab protein localization and function. Substitution of different pre...

journal_title：Molecular biology of the cell

authors： Calero M,Chen CZ,Zhu W,Winand N,Havas KA,Gilbert PM,Burd CG,Collins RN

更新日期：2003-05-01 00:00:00

abstract：:The perinucleolar compartment (PNC) is a nuclear substructure present in transformed cells. The PNC is defined by high concentrations of certain RNA binding proteins and a subset of small RNAs transcribed by RNA polymerase III (pol III), including the signal recognition particle RNA and an Alu RNA as reported here. To...

journal_title：Molecular biology of the cell

authors： Wang C,Politz JC,Pederson T,Huang S

更新日期：2003-06-01 00:00:00

abstract：:The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

journal_title：Molecular biology of the cell

authors： Melvin A,Mudie S,Rocha S

更新日期：2011-11-01 00:00:00

abstract：:Motile cells such as bacteria, amoebae, and fibroblasts display a continual level of energy-consuming reactions involving the cytoskeleton and signal pathways, regardless of whether or not they are actually migrating. I draw parallels between these "silent signals" and the intrinsic activity of the human brain, especi...

journal_title：Molecular biology of the cell

authors： Bray D

更新日期：2014-03-01 00:00:00

abstract：:To test the "traction fiber" model for metaphase positioning of bivalents during meiosis, kinetochore fibers of maloriented bivalents, induced during recovery from cold arrest, were analyzed with a liquid crystal polarizing microscope. The measured birefringence retardation of kinetochore fibers is proportional to the...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R

更新日期：2004-12-01 00:00:00

abstract：:Stearoyl-coenzyme A desaturase (SCD) is a key regulator of membrane fluidity, turns over rapidly, and represents a prototype for selective degradation of resident proteins of the endoplasmic reticulum. Using detergent-solubilized, desaturase-induced rat liver microsomes we have characterized a protease that degrades S...

journal_title：Molecular biology of the cell

authors： Heinemann FS,Ozols J

更新日期：1998-12-01 00:00:00

abstract：:Endosomal trafficking of signaling proteins plays an essential role in cellular homeostasis. The seven-pass transmembrane protein Frizzled (Fz) is a critical component of Wnt signaling. Although Wnt signaling is proposed to be regulated by endosomal trafficking of Fz, the molecular events that enable this regulation a...

journal_title：Molecular biology of the cell

authors： Pradhan-Sundd T,Verheyen EM

更新日期：2015-09-15 00:00:00

abstract：:Select lipid-anchored proteins such as glycosylphosphatidylinositol (GPI)-anchored proteins and nonreceptor tyrosine kinases may preferentially partition into sphingomyelin-rich and cholesterol-rich plasmalemmal microdomains, thereby acquiring resistance to detergent extraction. Two such domains, caveolae and lipid ra...

journal_title：Molecular biology of the cell

authors： Oh P,Schnitzer JE

更新日期：2001-03-01 00:00:00

abstract：:Dopamine (DA) increases Na(+),K(+)-ATPase activity in lung alveolar epithelial cells. This effect is associated with an increase in Na(+),K(+)-ATPase molecules within the plasma membrane (). Analysis of Na(+),K(+)-ATPase motion was performed in real-time in alveolar cells stably expressing Na(+),K(+)-ATPase molecules ...

journal_title：Molecular biology of the cell

authors： Bertorello AM,Komarova Y,Smith K,Leibiger IB,Efendiev R,Pedemonte CH,Borisy G,Sznajder JI

更新日期：2003-03-01 00:00:00

abstract：:Ca2+-triggered exocytosis of synaptic vesicles is controlled by the Ca2+-binding protein synaptotagmin (syt) I. Fifteen additional isoforms of syt have been identified. Here, we compared the abilities of three syt isoforms (I, VII, and IX) to regulate soluble N-ethylmaleimide-sensitive factor attachment protein recept...

journal_title：Molecular biology of the cell

authors： Bhalla A,Tucker WC,Chapman ER

更新日期：2005-10-01 00:00:00

abstract：:Melanosomes are tissue-specific organelles within which melanin is synthesized and stored. The melanocyte-specific glycoprotein Pmel17 is enriched in the lumen of premelanosomes, where it associates with characteristic striations of unknown composition upon which melanin is deposited. However, Pmel17 is synthesized as...

journal_title：Molecular biology of the cell

authors： Berson JF,Harper DC,Tenza D,Raposo G,Marks MS

更新日期：2001-11-01 00:00:00

abstract：:Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

journal_title：Molecular biology of the cell

authors： Mohi MG,Arai Ki,Watanabe S

更新日期：1998-12-01 00:00:00

abstract：:The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secre...

journal_title：Molecular biology of the cell

authors： Müsch A,Cohen D,Yeaman C,Nelson WJ,Rodriguez-Boulan E,Brennwald PJ

更新日期：2002-01-01 00:00:00

abstract：:Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the sali...

journal_title：Molecular biology of the cell

authors： Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

更新日期：2019-02-01 00:00:00