Dual prenylation is required for Rab protein localization and function.


:The majority of Rab proteins are posttranslationally modified with two geranylgeranyl lipid moieties that enable their stable association with membranes. In this study, we present evidence to demonstrate that there is a specific lipid requirement for Rab protein localization and function. Substitution of different prenyl anchors on Rab GTPases does not lead to correct function. In the case of YPT1 and SEC4, two essential Rab genes in Saccharomyces cerevisiae, alternative lipid tails cannot support life when present as the sole source of YPT1 and SEC4. Furthermore, our data suggest that double geranyl-geranyl groups are required for Rab proteins to correctly localize to their characteristic organelle membrane. We have identified a factor, Yip1p that specifically binds the di-geranylgeranylated Rab and does not interact with mono-prenylated Rab proteins. This is the first demonstration that the double prenylation modification of Rab proteins is an important feature in the function of this small GTPase family and adds specific prenylation to the already known determinants of Rab localization.


Mol Biol Cell


Calero M,Chen CZ,Zhu W,Winand N,Havas KA,Gilbert PM,Burd CG,Collins RN





Has Abstract


2003-05-01 00:00:00














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    authors: Segal M,Bloom K,Reed SI

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    authors: Su CH,McStay GP,Tzagoloff A

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    authors: Nagpal H,Hori T,Furukawa A,Sugase K,Osakabe A,Kurumizaka H,Fukagawa T

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    authors: Carrillo E,Ben-Ari G,Wildenhain J,Tyers M,Grammentz D,Lee TA

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Verdaasdonk JS,Gardner R,Stephens AD,Yeh E,Bloom K

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lee K,Kenny AE,Rieder CL

    更新日期:2010-07-01 00:00:00

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Giot L,Konopka JB

    更新日期:1997-06-01 00:00:00

  • The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation.

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    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Uzarska MA,Dutkiewicz R,Freibert SA,Lill R,Mühlenhoff U

    更新日期:2013-06-01 00:00:00