Yeast exocytic v-SNAREs confer endocytosis.

abstract：:The assembly of metazoan Sm-class small nuclear ribonucleoproteins (snRNPs) is an elaborate, step-wise process that takes place in multiple subcellular compartments. The initial steps, including formation of the core RNP, are mediated by the survival motor neuron (SMN) protein complex. Loss-of-function mutations in hu...

journal_title：Molecular biology of the cell

authors： Shpargel KB,Praveen K,Rajendra TK,Matera AG

更新日期：2009-01-01 00:00:00

abstract：:CWH41, a gene involved in the assembly of cell wall beta-1,6-glucan, has recently been shown to be the structural gene for Saccharomyces cerevisiae glucosidase I that is responsible for initiating the trimming of terminal alpha-1,2-glucose residue in the N-glycan processing pathway. To distinguish between a direct or ...

journal_title：Molecular biology of the cell

authors： Abeijon C,Chen LY

更新日期：1998-10-01 00:00:00

abstract：:Arg8-vasopressin (AVP) promotes the differentiation of myogenic cell lines and mouse primary satellite cells by mechanisms involving the transcriptional activation of myogenic bHLH regulatory factors and myocyte enhancer factor 2 (MEF2). We here report that AVP treatment of L6 cells results in the activation of calcin...

journal_title：Molecular biology of the cell

authors： Scicchitano BM,Spath L,Musarò A,Molinaro M,Rosenthal N,Nervi C,Adamo S

更新日期：2005-08-01 00:00:00

abstract：:We have studied telomere length in Schizosaccharomyces pombe strains carrying mutations affecting cell cycle checkpoints, DNA repair, and regulation of the Cdc2 protein kinase. Telomere shortening was found in rad1, rad3, rad17, and rad26 mutants. Telomere lengths in previously characterized rad1 mutants paralleled th...

journal_title：Molecular biology of the cell

authors： Dahlen M,Olsson T,Kanter-Smoler G,Ramne A,Sunnerhagen P

更新日期：1998-03-01 00:00:00

abstract：:Starvation induces Dictyostelium amoebae to secrete cAMP, toward which other amoebae stream, forming multicellular mounds that differentiate and develop into fruiting bodies containing spores. We find that the double deletion of cortexillin (ctx) I and II alters the actin cytoskeleton and substantially inhibits all mo...

journal_title：Molecular biology of the cell

authors： Shu S,Liu X,Kriebel PW,Daniels MP,Korn ED

更新日期：2012-01-01 00:00:00

abstract：:Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblas...

journal_title：Molecular biology of the cell

authors： Laulagnier K,Schieber NL,Maritzen T,Haucke V,Parton RG,Gruenberg J

更新日期：2011-06-15 00:00:00

abstract：:As yeast cultures enter stationary phase in rich, glucose-based medium, differentiation of two major subpopulations of cells, termed quiescent and nonquiescent, is observed. Differences in mRNA abundance between exponentially growing and stationary-phase cultures and quiescent and nonquiescent cells are known, but lit...

journal_title：Molecular biology of the cell

authors： Davidson GS,Joe RM,Roy S,Meirelles O,Allen CP,Wilson MR,Tapia PH,Manzanilla EE,Dodson AE,Chakraborty S,Carter M,Young S,Edwards B,Sklar L,Werner-Washburne M

更新日期：2011-04-01 00:00:00

abstract：:Oncoprotein 18/stathmin (Op18) has been identified recently as a protein that destabilizes microtubules, but the mechanism of destabilization is currently controversial. Based on in vitro microtubule assembly assays, evidence has been presented supporting conflicting destabilization models of either tubulin sequestrat...

journal_title：Molecular biology of the cell

authors： Howell B,Larsson N,Gullberg M,Cassimeris L

更新日期：1999-01-01 00:00:00

abstract：:Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

journal_title：Molecular biology of the cell

authors： Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

更新日期：2018-11-15 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcrip...

journal_title：Molecular biology of the cell

authors： González-Aguilera C,Tous C,Babiano R,de la Cruz J,Luna R,Aguilera A

更新日期：2011-08-01 00:00:00

abstract：:The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at e...

journal_title：Molecular biology of the cell

authors： Buttery SM,Yoshida S,Pellman D

更新日期：2007-05-01 00:00:00

abstract：:Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the p...

journal_title：Molecular biology of the cell

authors： Assaad FF,Qiu JL,Youngs H,Ehrhardt D,Zimmerli L,Kalde M,Wanner G,Peck SC,Edwards H,Ramonell K,Somerville CR,Thordal-Christensen H

更新日期：2004-11-01 00:00:00

abstract：:How recycling receptors are segregated from down-regulated receptors in the endosome is unknown. In previous studies, we demonstrated that substitutions in the transferrin receptor (TR) transmembrane domain (TM) convert the protein from an efficiently recycling receptor to one that is rapidly down regulated. In this s...

journal_title：Molecular biology of the cell

authors： Zaliauskiene L,Kang S,Brouillette CG,Lebowitz J,Arani RB,Collawn JF

更新日期：2000-08-01 00:00:00

abstract：:Functional proteins of complex eukaryotes within the same species are rather invariant. A single catalytic component of telomerase TERT is essential for an active telomerase complex that maintains telomeres. Surprisingly, we have identified two paralogous SpTERT-L and SpTERT-S genes with novel domains in Strongylocent...

journal_title：Molecular biology of the cell

authors： Wells TB,Zhang G,Harley Z,Vaziri H

更新日期：2009-01-01 00:00:00

abstract：:Myosin Va is associated with discrete vesicle populations in a number of cell types, but little is known of the function of myosin Vb. Yeast two-hybrid screening of a rabbit parietal cell cDNA library with dominant active Rab11a (Rab11aS20V) identified myosin Vb as an interacting protein for Rab11a, a marker for plasm...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Kumar R,Hales CM,Navarre J,Bhartur SG,Burnette JO,Provance DW Jr,Mercer JA,Bähler M,Goldenring JR

更新日期：2001-06-01 00:00:00

abstract：:The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein,...

journal_title：Molecular biology of the cell

authors： Nagy G,Milosevic I,Mohrmann R,Wiederhold K,Walter AM,Sørensen JB

更新日期：2008-09-01 00:00:00

abstract：:Important aspects of cell cycle regulation are the checkpoints, which respond to a variety of cellular stresses to inhibit cell cycle progression and act as protective mechanisms to ensure genomic integrity. An increasing number of tumor suppressors are being demonstrated to have roles in checkpoint mechanisms, implyi...

journal_title：Molecular biology of the cell

authors： Qiu L,Burgess A,Fairlie DP,Leonard H,Parsons PG,Gabrielli BG

更新日期：2000-06-01 00:00:00

abstract：:Cortical granules are specialized organelles whose contents interact with the extracellular matrix of the fertilized egg to form the block to polyspermy. In sea urchins, the granule contents form a fertilization envelope (FE), and this construction is critically dependent upon protease activity. An autocatalytic serin...

journal_title：Molecular biology of the cell

authors： Haley SA,Wessel GM

更新日期：2004-05-01 00:00:00

abstract：:In pancreatic β-cells, glutamate dehydrogenase (GDH) modulates insulin secretion, although its function regarding specific secretagogues is unclear. This study investigated the role of GDH using a β-cell-specific GDH knockout mouse model, called βGlud1(-/-). The absence of GDH in islets isolated from βGlud1(-/-) mice ...

journal_title：Molecular biology of the cell

authors： Vetterli L,Carobbio S,Pournourmohammadi S,Martin-Del-Rio R,Skytt DM,Waagepetersen HS,Tamarit-Rodriguez J,Maechler P

更新日期：2012-10-01 00:00:00

abstract：:We identified a novel interaction between myosin VI and the GLUT1 transporter binding protein GLUT1CBP(GIPC1) and first proposed that as an adapter molecule it might function to couple vesicle-bound proteins to myosin VI movement. This study refines the model by identifying two myosin VI binding domains in the GIPC1 C...

journal_title：Molecular biology of the cell

authors： Reed BC,Cefalu C,Bellaire BH,Cardelli JA,Louis T,Salamon J,Bloecher MA,Bunn RC

更新日期：2005-09-01 00:00:00

abstract：:The ubiquitin-like hPLIC proteins can associate with proteasomes, and hPLIC overexpression can specifically interfere with ubiquitin-mediated proteolysis (Kleijnen et al., 2000). Because the hPLIC proteins can also interact with certain E3 ubiquitin protein ligases, they may provide a link between the ubiquitination a...

journal_title：Molecular biology of the cell

authors： Kleijnen MF,Alarcon RM,Howley PM

更新日期：2003-09-01 00:00:00

abstract：:In vertebrate cells, the nucleoporin Nup358/RanBP2 is a major component of the filaments that emanate from the nuclear pore complex into the cytoplasm. Nup358 forms a complex with SUMOylated RanGAP1, the GTPase activating protein for Ran. RanGAP1 plays a pivotal role in the establishment of a RanGTP gradient across th...

journal_title：Molecular biology of the cell

authors： Hutten S,Flotho A,Melchior F,Kehlenbach RH

更新日期：2008-05-01 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is facilitated by a precisely regulated burst of actin assembly. PtdIns(4,5)P2 is an important signaling lipid with conserved roles in CME and actin assembly regulation. Rhomboid family multipass transmembrane proteins regulate diverse cellular processes; however, rhomboid-mediated ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Lewellyn EB,Drubin DG

更新日期：2015-04-15 00:00:00

abstract：:Isu, the scaffold protein on which Fe-S clusters are built in the mitochondrial matrix, plays a central role in the biogenesis of Fe-S cluster proteins. We report that the reduction in the activity of several components of the cluster biogenesis system, including the specialized Hsp70 Ssq1, causes a 15-20-fold up-regu...

journal_title：Molecular biology of the cell

authors： Andrew AJ,Song JY,Schilke B,Craig EA

更新日期：2008-12-01 00:00:00

abstract：:We recently reported that SMAP1, a GTPase-activating protein (GAP) for Arf6, directly interacts with clathrin and regulates the clathrin-dependent endocytosis of transferrin receptors from the plasma membrane. Here, we identified a SMAP1 homologue that we named SMAP2. Like SMAP1, SMAP2 exhibits GAP activity and intera...

journal_title：Molecular biology of the cell

authors： Natsume W,Tanabe K,Kon S,Yoshida N,Watanabe T,Torii T,Satake M

更新日期：2006-06-01 00:00:00

abstract：:Endoplasmic reticulum (ER) tubules and sheets conventionally correspond to smooth and rough ER, respectively. The ratio of ER tubules-to-sheets varies in different cell types and changes in response to cellular conditions, potentially impacting the functional output of the ER. To directly test whether ER morphology im...

journal_title：Molecular biology of the cell

authors： Mukherjee RN,Levy DL

更新日期：2019-08-15 00:00:00

abstract：:Compositional analyses of nucleic acids and proteins have shed light on possible origins of living cells. In this work, rigorous compositional analyses of ∼5000 plasma membrane lipid constituents of 273 species in the three life domains (archaea, eubacteria, and eukaryotes) revealed a remarkable statistical paradox, i...

journal_title：Molecular biology of the cell

authors： Bansal S,Mittal A

更新日期：2015-04-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00

abstract：:Forces generated by heart muscle contraction must be balanced by adhesion to the extracellular matrix (ECM) and to other cells for proper heart function. Decades of data have suggested that cell-ECM adhesions are important for sarcomere assembly. However, the relationship between cell-ECM adhesions and sarcomeres asse...

journal_title：Molecular biology of the cell

authors： Taneja N,Neininger AC,Burnette DT

更新日期：2020-06-01 00:00:00