Reticulon 4a promotes exocytosis in mammalian cells.

abstract：:The β4 isoform of the β-subunits of voltage-gated calcium channel regulates cell proliferation and cell cycle progression. Herein we show that coexpression of the β4-subunit with actors of the canonical Wnt/β-catenin signaling pathway in a hepatoma cell line inhibits Wnt-responsive gene transcription and decreases cel...

journal_title：Molecular biology of the cell

authors： Rima M,Daghsni M,Lopez A,Fajloun Z,Lefrancois L,Dunach M,Mori Y,Merle P,Brusés JL,De Waard M,Ronjat M

更新日期：2017-12-01 00:00:00

abstract：:Neural cell adhesion molecules (CAMs) are important players during neurogenesis and neurite outgrowth as well as axonal fasciculation and pathfinding. Some of these developmental processes entail the activation of cellular signaling cascades. Pharmacological and genetic evidence indicates that the neurite outgrowth-pr...

journal_title：Molecular biology of the cell

authors： Islam R,Kristiansen LV,Romani S,Garcia-Alonso L,Hortsch M

更新日期：2004-04-01 00:00:00

abstract：:Griscelli syndrome type 2 (GS2) is a genetic disorder in which patients exhibit life-threatening defects of cytotoxic T lymphocytes (CTLs) whose lytic granules fail to dock on the plasma membrane and therefore do not release their contents. The disease is caused by the absence of functional rab27a, but how rab27a cont...

journal_title：Molecular biology of the cell

authors： Neeft M,Wieffer M,de Jong AS,Negroiu G,Metz CH,van Loon A,Griffith J,Krijgsveld J,Wulffraat N,Koch H,Heck AJ,Brose N,Kleijmeer M,van der Sluijs P

更新日期：2005-02-01 00:00:00

abstract：:The tight junction adaptor protein ZO-1 regulates intracellular signaling and cell proliferation. Its Src homology 3 (SH3) domain is required for the regulation of proliferation and binds to the Y-box transcription factor ZO-1-associated nucleic acid binding protein (ZONAB). Binding of ZO-1 to ZONAB results in cytopla...

journal_title：Molecular biology of the cell

authors： Tsapara A,Matter K,Balda MS

更新日期：2006-03-01 00:00:00

abstract：:Maturation-promoting factor, consisting of cdc2 protein kinase and a regulatory B-type cyclin, is a universal regulator of meiosis and mitosis in eukaryotes. In Xenopus, there are two subtypes of B-type cyclins, designated B1 and B2, both of which are phosphorylated. In this study, we have investigated the biological ...

journal_title：Molecular biology of the cell

authors： Li J,Meyer AN,Donoghue DJ

更新日期：1995-09-01 00:00:00

abstract：:The intracellular trafficking of the epidermal growth factor receptor (EGFR) is regulated by a cross-talk between calmodulin (CaM) and protein kinase Cdelta (PKCdelta). On inhibition of CaM, PKCdelta promotes the formation of enlarged early endosomes and blocks EGFR recycling and degradation. Here, we show that PKCdel...

journal_title：Molecular biology of the cell

authors： Lladó A,Timpson P,Vilà de Muga S,Moretó J,Pol A,Grewal T,Daly RJ,Enrich C,Tebar F

更新日期：2008-01-01 00:00:00

abstract：:The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...

journal_title：Molecular biology of the cell

authors： Yang CH,Snyder M

更新日期：1992-11-01 00:00:00

abstract：:In epithelial cell lines, apical but not basolateral clathrin-mediated endocytosis has been shown to be affected by actin-disrupting drugs. Using electron and fluorescence microscopy, as well as biochemical assays, we show that the amount of actin dedicated to endocytosis is limiting at the apical surface of epithelia...

journal_title：Molecular biology of the cell

authors： Hyman T,Shmuel M,Altschuler Y

更新日期：2006-01-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p)...

journal_title：Molecular biology of the cell

authors： Trueman SF,Mandon EC,Gilmore R

更新日期：2011-09-01 00:00:00

abstract：:Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle- and cell adhesion-related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morphol...

journal_title：Molecular biology of the cell

authors： Kerosuo L,Bronner ME

更新日期：2014-02-01 00:00:00

abstract：:Members of the adenine nucleotide translocase (ANT) family exchange ADP for ATP across the mitochondrial inner membrane, an activity that is essential for oxidative phosphorylation (OXPHOS). Mutations in or dysregulation of ANTs is associated with progressive external ophthalmoplegia, cardiomyopathy, nonsyndromic inte...

journal_title：Molecular biology of the cell

authors： Lu YW,Acoba MG,Selvaraju K,Huang TC,Nirujogi RS,Sathe G,Pandey A,Claypool SM

更新日期：2017-06-01 00:00:00

abstract：:In mammalian cells, the inheritance of the Golgi apparatus into the daughter cells during each cycle of cell division is mediated by a disassembly and reassembly process, and this process is precisely controlled by phosphorylation and ubiquitination. VCIP135 (valosin-containing protein p97/p47 complex-interacting prot...

journal_title：Molecular biology of the cell

authors： Zhang X,Wang Y

更新日期：2015-06-15 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITPs) regulate the interface between lipid metabolism and specific steps in membrane trafficking through the secretory pathway in eukaryotes. Herein, we describe the cis-acting information that controls PITPbeta localization in mammalian cells. We demonstrate PITPbeta localizes...

journal_title：Molecular biology of the cell

authors： Phillips SE,Ile KE,Boukhelifa M,Huijbregts RP,Bankaitis VA

更新日期：2006-06-01 00:00:00

abstract：:Transport of cellular and neuronal vesicles, organelles, and other particles along microtubules requires the molecular motor protein dynein (Mallik and Gross, 2004). Critical to dynein function is dynactin, a multiprotein complex commonly thought to be required for dynein attachment to membrane compartments (Karki and...

journal_title：Molecular biology of the cell

authors： Haghnia M,Cavalli V,Shah SB,Schimmelpfeng K,Brusch R,Yang G,Herrera C,Pilling A,Goldstein LS

更新日期：2007-06-01 00:00:00

abstract：:Lamellipodia of crawling cells represent both the motor for cell advance and the primary building site for the actin cytoskeleton. The organization of actin in the lamellipodium reflects actin dynamics and is of critical importance for the mechanism of cell motility. In previous structural studies, the lamellipodial a...

journal_title：Molecular biology of the cell

authors： Verkhovsky AB,Chaga OY,Schaub S,Svitkina TM,Meister JJ,Borisy GG

更新日期：2003-11-01 00:00:00

abstract：:"Lipid raft" is the name given to the tiny, dynamic, and ordered domains of cholesterol and sphingolipids that are hypothesized to exist in the plasma membranes of eukaryotic cells. According to the lipid raft hypothesis, these cholesterol- and sphingolipid-enriched domains modulate the protein-protein interactions th...

journal_title：Molecular biology of the cell

authors： Kraft ML

更新日期：2013-09-01 00:00:00

abstract：:TPX2 is a Ran-regulated spindle assembly factor that is required for kinetochore fiber formation and activation of the mitotic kinase Aurora A. TPX2 is enriched near spindle poles and is required near kinetochores, suggesting that it undergoes dynamic relocalization throughout mitosis. Using photoactivation, we measur...

journal_title：Molecular biology of the cell

authors： Ma N,Tulu US,Ferenz NP,Fagerstrom C,Wilde A,Wadsworth P

更新日期：2010-03-15 00:00:00

abstract：:Despite the importance of epithelial cell contacts in determining cell behavior, we still lack a detailed understanding of the assembly and disassembly of intercellular contacts. Here we examined the role of the catalytic activity of the Src family kinases at epithelial cell contacts in vitro. Like E- and P-cadherin, ...

journal_title：Molecular biology of the cell

authors： Owens DW,McLean GW,Wyke AW,Paraskeva C,Parkinson EK,Frame MC,Brunton VG

更新日期：2000-01-01 00:00:00

abstract：:Glycerol and other polyols are used as osmoprotectants by many organisms. Several yeasts and other fungi can take up glycerol by proton symport. To identify genes involved in active glycerol uptake in Saccharomyces cerevisiae we screened a deletion mutant collection comprising 321 genes encoding proteins with 6 or mor...

journal_title：Molecular biology of the cell

authors： Ferreira C,van Voorst F,Martins A,Neves L,Oliveira R,Kielland-Brandt MC,Lucas C,Brandt A

更新日期：2005-04-01 00:00:00

abstract：:Wnt signaling plays key roles in embryonic development and adult stem cell homeostasis and is altered in human cancer. Signaling is turned on and off by regulating stability of the effector β-catenin (β-cat). The multiprotein destruction complex binds and phosphorylates β-cat and transfers it to the SCF-TrCP E3-ubiqui...

journal_title：Molecular biology of the cell

authors： Schaefer KN,Pronobis MI,Williams CE,Zhang S,Bauer L,Goldfarb D,Yan F,Major MB,Peifer M

更新日期：2020-05-01 00:00:00

abstract：:DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we express...

journal_title：Molecular biology of the cell

authors： Lee HO,Zacharek SJ,Xiong Y,Duronio RJ

更新日期：2010-11-01 00:00:00

abstract：:Five or more orthophosphates bound together by high-energy phosphoanhydride bonds are highly ubiquitous inorganic molecules called polyphosphate. Polyphosphate acts as a signaling molecule eliciting a number of responses in eukaryotic cells, but the mechanisms mediating these effects are poorly understood. Proliferati...

journal_title：Molecular biology of the cell

authors： Suess PM,Tang Y,Gomer RH

更新日期：2019-04-15 00:00:00

abstract：:Cyclins A and E and their partner cyclin-dependent kinases (Cdks) are key regulators of DNA synthesis and of mitosis. Immunofluorescence studies have shown that both cyclins are nuclear and that a proportion of cyclin A is localized to sites of DNA replication. However, recently, both cyclin A and cyclin E have been i...

journal_title：Molecular biology of the cell

authors： Jackman M,Kubota Y,den Elzen N,Hagting A,Pines J

更新日期：2002-03-01 00:00:00

abstract：:Ubiquitinated integral membrane proteins are delivered to the interior of the lysosome/vacuole for degradation. This process relies on specific ubiquitination of potential cargo and recognition of that Ub-cargo by sorting receptors at multiple compartments. We show that the endosomal Hse1-Vps27 sorting receptor binds ...

journal_title：Molecular biology of the cell

authors： Ren J,Kee Y,Huibregtse JM,Piper RC

更新日期：2007-01-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00

abstract：:Proteins of the SNARE (soluble N-ethylmalemide-sensitive factor attachment protein receptor) family are essential for the fusion of transport vesicles with an acceptor membrane. Despite considerable sequence divergence, their mechanism of action is conserved: heterologous sets assemble into membrane-bridging SNARE com...

journal_title：Molecular biology of the cell

authors： Kloepper TH,Kienle CN,Fasshauer D

更新日期：2007-09-01 00:00:00

abstract：:The daily phagocytosis of shed photoreceptor outer segments by pigment epithelial cells is critical for the maintenance of the retina. In a subtractive polymerase chain reaction analysis, we found that functional differentiation of human ARPE19 retinal pigment epithelial (RPE) cells is accompanied by up-regulation of ...

journal_title：Molecular biology of the cell

authors： Law AL,Ling Q,Hajjar KA,Futter CE,Greenwood J,Adamson P,Wavre-Shapton ST,Moss SE,Hayes MJ

更新日期：2009-09-01 00:00:00

abstract：:The anaphase-promoting complex in partnership with its activator, Cdh1, is an E3 ubiquitin ligase responsible for targeting cell cycle proteins during G1 phase. In the budding yeast Saccharomyces cerevisiae, Cdh1 associates with the deubiquitinating enzyme Ubp15, but the significance of this interaction is unclear. To...

journal_title：Molecular biology of the cell

authors： Ostapenko D,Burton JL,Solomon MJ

更新日期：2015-06-15 00:00:00