An elaborate classification of SNARE proteins sheds light on the conservation of the eukaryotic endomembrane system.

abstract：:Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

journal_title：Molecular biology of the cell

authors： Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

更新日期：2011-11-01 00:00:00

abstract：:The auxilin family of J-domain proteins load Hsp70 onto clathrin-coated vesicles (CCVs) to drive uncoating. In vitro, auxilin function requires its ability to bind clathrin and stimulate Hsp70 ATPase activity via its J-domain. To test these requirements in vivo, we performed a mutational analysis of Swa2p, the yeast a...

journal_title：Molecular biology of the cell

authors： Xiao J,Kim LS,Graham TR

更新日期：2006-07-01 00:00:00

abstract：:Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

journal_title：Molecular biology of the cell

authors： Soldati T,Riederer MA,Pfeffer SR

更新日期：1993-04-01 00:00:00

abstract：:Spa2p is a nonessential protein that regulates yeast cell polarity. It localizes early to the presumptive bud site and remains at sites of growth throughout the cell cycle. To understand how Spa2p localization is regulated and to gain insight into its molecular function in cell polarity, we used a coimmunoprecipitatio...

journal_title：Molecular biology of the cell

authors： Shih JL,Reck-Peterson SL,Newitt R,Mooseker MS,Aebersold R,Herskowitz I

更新日期：2005-10-01 00:00:00

abstract：:Colony-stimulating factor-1 (CSF-1) induces expression of immediate early gene, such as c-myc and c-fos and delayed early genes such as D-type cyclins (D1 and D2), whose products play essential roles in the G1 to S phase transition of the cell cycle. Little is known, however, about the cytoplasmic signal transduction ...

journal_title：Molecular biology of the cell

authors： Dey A,She H,Kim L,Boruch A,Guris DL,Carlberg K,Sebti SM,Woodley DT,Imamoto A,Li W

更新日期：2000-11-01 00:00:00

abstract：:A primary cilium is found on most mammalian cells, where it acts as a cellular antenna for the reception of both mechanical and chemical signals. A variety of diseases are associated with defective ciliogenesis, reflecting the ubiquity of the function of cilia and the number of proteins required for their assembly. Pr...

journal_title：Molecular biology of the cell

authors： Copeland SJ,McRae A,Guarguaglini G,Trinkle-Mulcahy L,Copeland JW

更新日期：2018-07-01 00:00:00

abstract：:The NUDF protein of the filamentous fungus Aspergillus nidulans functions in the cytoplasmic dynein pathway. It binds several proteins, including the NUDE protein. Green fluorescent protein-tagged NUDF and NUDA (dynein heavy chain) localize to linearly moving dashes ("comets") that coincide with microtubule ends. Here...

journal_title：Molecular biology of the cell

authors： Efimov VP

更新日期：2003-03-01 00:00:00

abstract：:Regulated exocytosis is thought to occur either by "full fusion," where the secretory vesicle fuses with the plasma membrane (PM) via a fusion pore that then dilates until the secretory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and instead rapidly reseals such that the ...

journal_title：Molecular biology of the cell

authors： Sokac AM,Bement WM

更新日期：2006-04-01 00:00:00

abstract：:Protein degradation during the cell cycle is controlled by the opposing activities of ubiquitin ligases and deubiquitinating enzymes (DUBs). Although the functions of ubiquitin ligases in the cell cycle have been studied extensively, the roles of DUBs in this process are less well understood. Here, we used an overexpr...

journal_title：Molecular biology of the cell

authors： Mapa CE,Arsenault HE,Conti MM,Poti KE,Benanti JA

更新日期：2018-11-15 00:00:00

abstract：:Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic ...

journal_title：Molecular biology of the cell

authors： Faigle W,Colucci-Guyon E,Louvard D,Amigorena S,Galli T

更新日期：2000-10-01 00:00:00

abstract：:A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in...

journal_title：Molecular biology of the cell

authors： Jantsch V,Tang L,Pasierbek P,Penkner A,Nayak S,Baudrimont A,Schedl T,Gartner A,Loidl J

更新日期：2007-12-01 00:00:00

abstract：:In this study, we examined the subcellular distribution and functions of LIMK1 in developing neurons. Confocal microscopy, subcellular fractionation, and expression of several epitope-tagged LIMK1 constructs revealed that LIMK1 is enriched in the Golgi apparatus and growth cones, with the LIM domain required for Golgi...

journal_title：Molecular biology of the cell

authors： Rosso S,Bollati F,Bisbal M,Peretti D,Sumi T,Nakamura T,Quiroga S,Ferreira A,Cáceres A

更新日期：2004-07-01 00:00:00

abstract：:The membrane-trafficking pathway mediated by tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) in neurons is still unknown. We show herein that TI-VAMP expression is necessary for neurite outgrowth in PC12 cells and hippocampal neurons in culture. TI-VAMP interacts with plasma membrane and e...

journal_title：Molecular biology of the cell

authors： Alberts P,Rudge R,Hinners I,Muzerelle A,Martinez-Arca S,Irinopoulou T,Marthiens V,Tooze S,Rathjen F,Gaspar P,Galli T

更新日期：2003-10-01 00:00:00

abstract：:Despite the fact that the chromosomal passenger complex is well known to regulate kinetochore behavior in mitosis, no functional link has yet been established between the complex and kinetochore structure. In addition, remarkably little is known about how the complex targets to centromeres. Here, in a study of caspase...

journal_title：Molecular biology of the cell

authors： Faragher AJ,Sun XM,Butterworth M,Harper N,Mulheran M,Ruchaud S,Earnshaw WC,Cohen GM

更新日期：2007-04-01 00:00:00

abstract：:Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. When transiently expressed as fluorescent protein (FP) fusions, the three PP1 isoforms, alpha, beta/delta, and gamma1, are active phosphatases with distinct localization patterns. W...

journal_title：Molecular biology of the cell

authors： Trinkle-Mulcahy L,Andrews PD,Wickramasinghe S,Sleeman J,Prescott A,Lam YW,Lyon C,Swedlow JR,Lamond AI

更新日期：2003-01-01 00:00:00

abstract：:Eukaryotic cells activate the unfolded-protein response (UPR) upon endoplasmic reticulum (ER) stress, where the stress is assumed to be the accumulation of unfolded proteins in the ER. Consistent with previous in vitro studies of the ER-luminal domain of the mutant UPR initiator Ire1, our study show its association wi...

journal_title：Molecular biology of the cell

authors： Promlek T,Ishiwata-Kimata Y,Shido M,Sakuramoto M,Kohno K,Kimata Y

更新日期：2011-09-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:Jak tyrosine kinases have a unique domain structure containing a kinase domain (JH1) adjacent to a catalytically inactive pseudokinase domain (JH2). JH2 is crucial for inhibition of basal Jak activity, but the mechanism of this regulation has remained elusive. We show that JH2 negatively regulated Jak2 in bacterial ce...

journal_title：Molecular biology of the cell

authors： Saharinen P,Vihinen M,Silvennoinen O

更新日期：2003-04-01 00:00:00

abstract：:The Arf and Rab/Ypt GTPases coordinately regulate membrane traffic and organelle structure by regulating vesicle formation and fusion. Ample evidence has indicated that proteins in these two families may function in parallel or complementarily; however, the manner in which Arf and Rab/Ypt proteins perform interchangea...

journal_title：Molecular biology of the cell

authors： Chen YT,Wang IH,Wang YH,Chiu WY,Hu JH,Chen WH,Lee FS

更新日期：2019-04-01 00:00:00

abstract：:The Wnt signaling pathway plays a major role in development, and upon deregulation it is implicated in neoplasia. The hallmark of the canonical Wnt signal is the protection of beta-catenin from ubiquitination and proteasomal degradation induced by glycogen synthase kinase (GSK)-3beta inhibition. The stabilized beta-ca...

journal_title：Molecular biology of the cell

authors： Bachar-Dahan L,Goltzmann J,Yaniv A,Gazit A

更新日期：2006-06-01 00:00:00

abstract：:The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

journal_title：Molecular biology of the cell

authors： Kalkan T,Iwasaki Y,Park CY,Thomsen GH

更新日期：2009-07-01 00:00:00

abstract：:RhoA is a small guanosine-5'-triphosphatase (GTPase) suggested to be essential for cytokinesis, stress fiber formation, and epithelial cell-cell contacts. In skin, loss of RhoA was suggested to underlie pemphigus skin blistering. To analyze RhoA function in vivo, we generated mice with a keratinocyte-restricted deleti...

journal_title：Molecular biology of the cell

authors： Jackson B,Peyrollier K,Pedersen E,Basse A,Karlsson R,Wang Z,Lefever T,Ochsenbein AM,Schmidt G,Aktories K,Stanley A,Quondamatteo F,Ladwein M,Rottner K,van Hengel J,Brakebusch C

更新日期：2011-03-01 00:00:00

abstract：:The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin...

journal_title：Molecular biology of the cell

authors： Shimi T,Kittisopikul M,Tran J,Goldman AE,Adam SA,Zheng Y,Jaqaman K,Goldman RD

更新日期：2015-11-05 00:00:00

abstract：:Mitochondrial dysfunction and altered transmembrane potential initiate a mitochondrial respiratory stress response, also known as mitochondrial retrograde response, in a wide spectrum of cells. The mitochondrial stress response activates calcineurin, which regulates transcription factors, including a new nuclear facto...

journal_title：Molecular biology of the cell

authors： Guha M,Pan H,Fang JK,Avadhani NG

更新日期：2009-09-01 00:00:00

abstract：:The nuclear import receptors importin β and transportin play a different role in mitosis: both act phenotypically as spatial regulators to ensure that mitotic spindle, nuclear membrane, and nuclear pore assembly occur exclusively around chromatin. Importin β is known to act by repressing assembly factors in regions di...

journal_title：Molecular biology of the cell

authors： Bernis C,Swift-Taylor B,Nord M,Carmona S,Chook YM,Forbes DJ

更新日期：2014-04-01 00:00:00

abstract：:Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

journal_title：Molecular biology of the cell

authors： Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

更新日期：2004-02-01 00:00:00

abstract：:During stem cell divisions, mitotic microtubules do more than just segregate the chromosomes. They also determine whether a cell divides virtually symmetrically or asymmetrically by establishing spindle orientation and the plane of cell division. This can be decisive for the fate of the stem cell progeny. Spindle defe...

journal_title：Molecular biology of the cell

authors： Mora-Bermúdez F,Huttner WB

更新日期：2015-12-01 00:00:00

abstract：:Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor over...

journal_title：Molecular biology of the cell

authors： Albee AJ,Wiese C

更新日期：2008-08-01 00:00:00

abstract：:Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disr...

journal_title：Molecular biology of the cell

authors： Zeng C,Anjard C,Riemann K,Konzok A,Nellen W

更新日期：2000-05-01 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is the major mechanism for internalization in mammalian cells. CME initiates by recruitment of adaptors and clathrin to form clathrin-coated pits (CCPs). Nearly half of nascent CCPs abort, whereas others are stabilized by unknown mechanisms and undergo further maturation before pinc...

journal_title：Molecular biology of the cell

authors： Antonescu CN,Aguet F,Danuser G,Schmid SL

更新日期：2011-07-15 00:00:00