Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain.

abstract：:Neuronal dynamics result from the integration of forces developed by molecular motors, especially conventional myosins. Myosin IIC is a recently discovered nonsarcomeric conventional myosin motor, the function of which is poorly understood, particularly in relation to the separate but coupled activities of its close h...

journal_title：Molecular biology of the cell

authors： Wylie SR,Chantler PD

更新日期：2008-09-01 00:00:00

abstract：:As yeast cultures enter stationary phase in rich, glucose-based medium, differentiation of two major subpopulations of cells, termed quiescent and nonquiescent, is observed. Differences in mRNA abundance between exponentially growing and stationary-phase cultures and quiescent and nonquiescent cells are known, but lit...

journal_title：Molecular biology of the cell

authors： Davidson GS,Joe RM,Roy S,Meirelles O,Allen CP,Wilson MR,Tapia PH,Manzanilla EE,Dodson AE,Chakraborty S,Carter M,Young S,Edwards B,Sklar L,Werner-Washburne M

更新日期：2011-04-01 00:00:00

abstract：:An in vitro transport assay, established with a modified Shiga toxin B subunit (STxB) as a marker, has proved to be useful for the study of transport from the early/recycling endosome (EE/RE) to the trans-Golgi network (TGN). Here, we modified this assay to test antibodies to all known soluble N-ethylmaleimide-sensiti...

journal_title：Molecular biology of the cell

authors： Tai G,Lu L,Wang TL,Tang BL,Goud B,Johannes L,Hong W

更新日期：2004-09-01 00:00:00

abstract：:ELMO2 belongs to a family of scaffold proteins involved in phagocytosis and cell motility. ELMO2 can simultaneously bind integrin-linked kinase (ILK) and RhoG, forming tripartite ERI complexes. These complexes are involved in promoting β1 integrin-dependent directional migration in undifferentiated epidermal keratinoc...

journal_title：Molecular biology of the cell

authors： Jackson BC,Ivanova IA,Dagnino L

更新日期：2015-07-15 00:00:00

abstract：:Focal adhesions are intricate protein complexes that facilitate cell attachment, migration, and cellular communication. Lasp-2 (LIM-nebulette), a member of the nebulin family of actin-binding proteins, is a newly identified component of these complexes. To gain further insights into the functional role of lasp-2, we i...

journal_title：Molecular biology of the cell

authors： Bliss KT,Chu M,Jones-Weinert CM,Gregorio CC

更新日期：2013-04-01 00:00:00

abstract：:Microtubule-associated proteins (MAPs) are a functionally highly diverse class of proteins that help to adjust the shape and function of the microtubule cytoskeleton in space and time. For this purpose, MAPs structurally support microtubules, modulate their dynamic instability, or regulate the activity of associated m...

journal_title：Molecular biology of the cell

authors： Drechsler H,Xu Y,Geyer VF,Zhang Y,Diez S

更新日期：2019-11-15 00:00:00

abstract：:The cohesin complex (Mcd1p, Smc1p, Smc3p, and Scc3p) has multiple roles in chromosome architecture, such as promoting sister chromatid cohesion, chromosome condensation, DNA repair, and transcriptional regulation. The prevailing embrace model for sister chromatid cohesion posits that a single cohesin complex entraps b...

journal_title：Molecular biology of the cell

authors： Eng T,Guacci V,Koshland D

更新日期：2015-11-15 00:00:00

abstract：:Despite the fact that the chromosomal passenger complex is well known to regulate kinetochore behavior in mitosis, no functional link has yet been established between the complex and kinetochore structure. In addition, remarkably little is known about how the complex targets to centromeres. Here, in a study of caspase...

journal_title：Molecular biology of the cell

authors： Faragher AJ,Sun XM,Butterworth M,Harper N,Mulheran M,Ruchaud S,Earnshaw WC,Cohen GM

更新日期：2007-04-01 00:00:00

abstract：:DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we express...

journal_title：Molecular biology of the cell

authors： Lee HO,Zacharek SJ,Xiong Y,Duronio RJ

更新日期：2010-11-01 00:00:00

abstract：:The plant toxin ricin is transported retrogradely from the cell surface to the endoplasmic reticulum (ER) from where the enzymatically active part is retrotranslocated to the cytosol, presumably by the same mechanism as used by misfolded proteins. The ER degradation enhancing alpha-mannosidase I-like protein, EDEM, is...

journal_title：Molecular biology of the cell

authors： Slominska-Wojewodzka M,Gregers TF,Wälchli S,Sandvig K

更新日期：2006-04-01 00:00:00

abstract：:After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing vacuoles (SCVs) undergo a maturation process that involves sequential acquisition of Rab5 and Rab7 and displacement toward the ...

journal_title：Molecular biology of the cell

authors： Harrison RE,Brumell JH,Khandani A,Bucci C,Scott CC,Jiang X,Finlay BB,Grinstein S

更新日期：2004-07-01 00:00:00

abstract：:Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

journal_title：Molecular biology of the cell

authors： Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

更新日期：2020-11-01 00:00:00

abstract：:Transporting epithelial cells optimize their morphology for solute uptake by building an apical specialization: a dense array of microvilli that serves to increase membrane surface area. In the intestinal tract, individual cells build thousands of microvilli, which pack tightly to form the brush border. Recent studies...

journal_title：Molecular biology of the cell

authors： Pinette JA,Mao S,Millis BA,Krystofiak ES,Faust JJ,Tyska MJ

更新日期：2019-01-01 00:00:00

abstract：:The human fungal pathogen Candida albicans can switch between yeast, pseudohyphal, and hyphal morphologies. To investigate whether the distinctive characteristics of hyphae are due to increased activity of the Cdc42 GTPase, strains lacking negative regulators of Cdc42 were constructed. Unexpectedly, the deletion of th...

journal_title：Molecular biology of the cell

authors： Court H,Sudbery P

更新日期：2007-01-01 00:00:00

abstract：:A novel selection scheme has been developed to isolate bloodstream forms of Trypanosoma brucei, which are defective in their ability to differentiate to the procyclic stage. Detailed characterization of one selected cell line (defective in differentiation clone 1 [DiD-1]) has demonstrated that these cells are indistin...

journal_title：Molecular biology of the cell

authors： Tasker M,Wilson J,Sarkar M,Hendriks E,Matthews K

更新日期：2000-05-01 00:00:00

abstract：:Multiple tau gene mutations are pathogenic for hereditary frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), with filamentous tau aggregates as the major lesions in the CNS of these patients. Recent studies have shown that bacterially expressed recombinant tau proteins with FTDP-17 missense mu...

journal_title：Molecular biology of the cell

authors： Vogelsberg-Ragaglia V,Bruce J,Richter-Landsberg C,Zhang B,Hong M,Trojanowski JQ,Lee VM

更新日期：2000-12-01 00:00:00

abstract：:Motile cells such as bacteria, amoebae, and fibroblasts display a continual level of energy-consuming reactions involving the cytoskeleton and signal pathways, regardless of whether or not they are actually migrating. I draw parallels between these "silent signals" and the intrinsic activity of the human brain, especi...

journal_title：Molecular biology of the cell

authors： Bray D

更新日期：2014-03-01 00:00:00

abstract：:Neural cell adhesion molecules (CAMs) are important players during neurogenesis and neurite outgrowth as well as axonal fasciculation and pathfinding. Some of these developmental processes entail the activation of cellular signaling cascades. Pharmacological and genetic evidence indicates that the neurite outgrowth-pr...

journal_title：Molecular biology of the cell

authors： Islam R,Kristiansen LV,Romani S,Garcia-Alonso L,Hortsch M

更新日期：2004-04-01 00:00:00

abstract：:Clathrin and caveolins are known for their involvement in the internalization of numerous receptors. Here we show that in polarized epithelial Madin-Darby canine kidney cells, both the clathrin machinery and caveolins are involved in the endocytosis and delivery to the plasma membrane (PM) of the M1 muscarinic acetylc...

journal_title：Molecular biology of the cell

authors： Shmuel M,Nodel-Berner E,Hyman T,Rouvinski A,Altschuler Y

更新日期：2007-05-01 00:00:00

abstract：:Integrin-mediated adhesion induces several signaling pathways leading to regulation of gene transcription, control of cell cycle entry and survival from apoptosis. Here we investigate the involvement of the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) pathway in integrin-mediated signal...

journal_title：Molecular biology of the cell

authors： Brizzi MF,Defilippi P,Rosso A,Venturino M,Garbarino G,Miyajima A,Silengo L,Tarone G,Pegoraro L

更新日期：1999-10-01 00:00:00

abstract：:During their migration to the periphery, cranial neural crest cells (NCCs) are repulsed by an ErbB4-dependent cue(s) in the mesenchyme adjoining rhombomeres (r) 3 and 5, which are segmented hindbrain neuromeres. ErbB4 has many ligands, but which ligand functions in the above system has not yet been clearly determined....

journal_title：Molecular biology of the cell

authors： Hoshino H,Uchida T,Otsuki T,Kawamoto S,Okubo K,Takeichi M,Chisaka O

更新日期：2007-04-01 00:00:00

abstract：:In kinetochores, the Ndc80 complex couples the energy in a depolymerizing microtubule to perform the work of moving chromosomes. The complex directly binds microtubules using an unstructured, positively charged N-terminal tail located on Hec1/Ndc80. Hec1/Ndc80 also contains a calponin homology domain (CHD) that increa...

journal_title：Molecular biology of the cell

authors： Tooley JG,Miller SA,Stukenberg PT

更新日期：2011-04-15 00:00:00

abstract：:Macrophages and dendritic cells exposed to lipopolysaccharide (LPS) convert their lysosomes from small, punctate organelles into a network of tubules. Tubular lysosomes have been implicated in phagosome maturation, retention of fluid phase, and antigen presentation. There is a growing appreciation that lysosomes act a...

journal_title：Molecular biology of the cell

authors： Saric A,Hipolito VE,Kay JG,Canton J,Antonescu CN,Botelho RJ

更新日期：2016-01-15 00:00:00

abstract：:Cysteine string protein (Csp) is essential for neurotransmitter release in Drosophila. It has been suggested that Csp functions by regulating the activity of presynaptic Ca2+ channels, thus controlling exocytosis. We have examined the effect of overexpressing Csp1 in PC12 cells, a neuroendocrine cell line. PC12 cell c...

journal_title：Molecular biology of the cell

authors： Chamberlain LH,Burgoyne RD

更新日期：1998-08-01 00:00:00

abstract：:During a search for genes controlling conidial dormancy in Aspergillus fumigatus, two dehydrin-like genes, DprA and DprB, were identified. The deduced proteins had repeated stretches of 23 amino acids that contained a conserved dehydrin-like protein (DPR) motif. Disrupted DprAΔ mutants were hypersensitive to oxidative...

journal_title：Molecular biology of the cell

authors： Wong Sak Hoi J,Lamarre C,Beau R,Meneau I,Berepiki A,Barre A,Mellado E,Read ND,Latgé JP

更新日期：2011-06-01 00:00:00

abstract：:Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outst...

journal_title：Molecular biology of the cell

authors： Graef M,Friedman JR,Graham C,Babu M,Nunnari J

更新日期：2013-09-01 00:00:00

abstract：:Human HT2-19 cells with a conditional cdk1 mutation stop dividing upon cdk1 inactivation and undergo multiple rounds of endoreplication. We show herein that major cell cycle events remain synchronized in these endoreplicating cells. DNA replication alternates with gap phases and cell cycle-specific cyclin E expression...

journal_title：Molecular biology of the cell

authors： Laronne A,Rotkopf S,Hellman A,Gruenbaum Y,Porter AC,Brandeis M

更新日期：2003-09-01 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00

abstract：:Members of the transforming acidic coiled coil (TACC) protein family are emerging as important mitotic spindle assembly proteins in a variety of organisms. The molecular details of how TACC proteins function are unknown, but TACC proteins have been proposed to recruit microtubule-stabilizing proteins of the tumor over...

journal_title：Molecular biology of the cell

authors： Albee AJ,Wiese C

更新日期：2008-08-01 00:00:00

abstract：:As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies, and worms, choanoflagellates have been refractory to DNA delivery methods for expressing foreign...

journal_title：Molecular biology of the cell

authors： Booth DS,Szmidt-Middleton H,King N

更新日期：2018-12-01 00:00:00