Activation of EGF receptor kinase by L1-mediated homophilic cell interactions.

abstract：:The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs....

journal_title：Molecular biology of the cell

authors： Mobarak CD,Anderson KD,Morin M,Beckel-Mitchener A,Rogers SL,Furneaux H,King P,Perrone-Bizzozero NI

更新日期：2000-09-01 00:00:00

abstract：:Ca(2+) is absorbed across intestinal epithelial monolayers via transcellular and paracellular pathways, and an active form of vitamin D(3), 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)], is known to promote intestinal Ca(2+) absorption. However, the molecules driving the paracellular Ca(2+) absorption and its...

journal_title：Molecular biology of the cell

authors： Fujita H,Sugimoto K,Inatomi S,Maeda T,Osanai M,Uchiyama Y,Yamamoto Y,Wada T,Kojima T,Yokozaki H,Yamashita T,Kato S,Sawada N,Chiba H

更新日期：2008-05-01 00:00:00

abstract：:A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

journal_title：Molecular biology of the cell

authors： Korobova F,Svitkina T

更新日期：2008-04-01 00:00:00

abstract：:More than 97% of mice in which the C-terminal region of connexin43 (Cx43) was removed (designated as Cx43K258stop) die shortly after birth due to a defect of the epidermal barrier. The abnormal expression of Cx43K258stop protein in the uppermost layers of the epidermis seems to perturb terminal differentiation of kera...

journal_title：Molecular biology of the cell

authors： Maass K,Ghanem A,Kim JS,Saathoff M,Urschel S,Kirfel G,Grümmer R,Kretz M,Lewalter T,Tiemann K,Winterhager E,Herzog V,Willecke K

更新日期：2004-10-01 00:00:00

abstract：:Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

journal_title：Molecular biology of the cell

authors： Klionsky DJ

更新日期：2016-03-01 00:00:00

abstract：:The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]....

journal_title：Molecular biology of the cell

authors： Kryndushkin D,Wickner RB

更新日期：2007-06-01 00:00:00

abstract：:MAL, a compact hydrophobic, four-transmembrane-domain apical protein that copurifies with detergent-resistant membranes is obligatory for the machinery that sorts glycophosphatidylinositol (GPI)-anchored proteins and others to the apical membrane in epithelia. The mechanism of MAL function in lipid-raft-mediated apica...

journal_title：Molecular biology of the cell

authors： Magal LG,Yaffe Y,Shepshelovich J,Aranda JF,de Marco Mdel C,Gaus K,Alonso MA,Hirschberg K

更新日期：2009-08-01 00:00:00

abstract：:HURP is a spindle-associated protein that mediates Ran-GTP-dependent assembly of the bipolar spindle and promotes chromosome congression and interkinetochore tension during mitosis. We report here a biochemical mechanism of HURP regulation by Aurora A, a key mitotic kinase that controls the assembly and function of th...

journal_title：Molecular biology of the cell

authors： Wong J,Lerrigo R,Jang CY,Fang G

更新日期：2008-05-01 00:00:00

abstract：:Emerging findings suggest that two lineages of mitochondrial Ca(2+) uptake participate during active and resting states: 1) the major eukaryotic membrane potential-dependent mitochondrial Ca(2+) uniporter and 2) the evolutionarily conserved exchangers and solute carriers, which are also involved in ion transport. Alth...

journal_title：Molecular biology of the cell

authors： Hoffman NE,Chandramoorthy HC,Shanmughapriya S,Zhang XQ,Vallem S,Doonan PJ,Malliankaraman K,Guo S,Rajan S,Elrod JW,Koch WJ,Cheung JY,Madesh M

更新日期：2014-03-01 00:00:00

abstract：:Cytokinesis in metazoan cells requires a set of antiparallel microtubules that become bundled upon anaphase onset to form a structure known as the central spindle. Bundling of these microtubules requires a protein complex, centralspindlin, that consists of the CYK-4/MgcRacGAP Rho-family GTPase-activating protein and t...

journal_title：Molecular biology of the cell

authors： Pavicic-Kaltenbrunner V,Mishima M,Glotzer M

更新日期：2007-12-01 00:00:00

abstract：:The receptor-tyrosine kinase (RTK)/Ras/Raf pathway is an essential cascade for mediating growth factor signaling. It is abnormally overactive in almost all human cancers. The downstream targets of the pathway are members of the extracellular regulated kinases (Erk1/2) family, suggesting that this family is a mediator ...

journal_title：Molecular biology of the cell

authors： Smorodinsky-Atias K,Goshen-Lago T,Goldberg-Carp A,Melamed D,Shir A,Mooshayef N,Beenstock J,Karamansha Y,Darlyuk-Saadon I,Livnah O,Ahn NG,Admon A,Engelberg D

更新日期：2016-03-15 00:00:00

abstract：:Werner syndrome (WS) is a human progeroid syndrome characterized by the early onset of a large number of clinical features associated with the normal aging process. The complex molecular and cellular phenotypes of WS involve characteristic features of genomic instability and accelerated replicative senescence. The gen...

journal_title：Molecular biology of the cell

authors： Balajee AS,Machwe A,May A,Gray MD,Oshima J,Martin GM,Nehlin JO,Brosh R,Orren DK,Bohr VA

更新日期：1999-08-01 00:00:00

abstract：:The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have ...

journal_title：Molecular biology of the cell

authors： Cai S,Song Y,Chen C,Shi J,Gan L

更新日期：2018-07-01 00:00:00

abstract：:On starvation, the cellular slime mold Dictyostelium discoideum initiates a program of development leading to formation of multicellular structures. The initial cell aggregation requires chemotaxis to cyclic AMP (cAMP) and relay of the cAMP signal by the activation of adenylyl cyclase (ACA), and it has been shown prev...

journal_title：Molecular biology of the cell

authors： Bolourani P,Spiegelman GB,Weeks G

更新日期：2006-10-01 00:00:00

abstract：:Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Pha...

journal_title：Molecular biology of the cell

authors： Faust JJ,Balabiyev A,Heddleston JM,Podolnikova NP,Baluch DP,Chew TL,Ugarova TP

更新日期：2019-08-01 00:00:00

abstract：:Low molecular weight (LMW) GTP-binding proteins are hypothesized to play a role in the vectorial transport of intracellular vesicles. Mutational studies in yeast and subcellular localization in mammalian cells suggest that a family of LMW GTP-binding proteins, termed rab, target intracellular vesicles to their appropr...

journal_title：Molecular biology of the cell

authors： Ngsee JK,Fleming AM,Scheller RH

更新日期：1993-07-01 00:00:00

abstract：:Macrophage polarization contributes to a number of human pathologies. This is exemplified for tumor-associated macrophages (TAMs), which display a polarized M2 phenotype, closely associated with promotion of angiogenesis and suppression of innate immune responses. We present evidence that induction of apoptosis in tum...

journal_title：Molecular biology of the cell

authors： Weigert A,Tzieply N,von Knethen A,Johann AM,Schmidt H,Geisslinger G,Brüne B

更新日期：2007-10-01 00:00:00

abstract：:In this study, we examined the subcellular distribution and functions of LIMK1 in developing neurons. Confocal microscopy, subcellular fractionation, and expression of several epitope-tagged LIMK1 constructs revealed that LIMK1 is enriched in the Golgi apparatus and growth cones, with the LIM domain required for Golgi...

journal_title：Molecular biology of the cell

authors： Rosso S,Bollati F,Bisbal M,Peretti D,Sumi T,Nakamura T,Quiroga S,Ferreira A,Cáceres A

更新日期：2004-07-01 00:00:00

abstract：:Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

journal_title：Molecular biology of the cell

authors： Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

更新日期：2014-09-15 00:00:00

abstract：:We identify a new mechanism for the beta(1)-adrenergic receptor (beta(1)AR)-mediated regulation of human ether-a-go-go-related gene (HERG) potassium channel (Kv11.1). We find that the previously reported modulatory interaction between Kv11.1 channels and 14-3-3epsilon proteins is competed by wild type beta(1)AR by mea...

journal_title：Molecular biology of the cell

authors： Tutor AS,Delpón E,Caballero R,Gómez R,Núñez L,Vaquero M,Tamargo J,Mayor F Jr,Penela P

更新日期：2006-11-01 00:00:00

abstract：:Mitochondrial decline is a hallmark of aging, and cells are equipped with many systems to regulate mitochondrial structure and function in response to stress and metabolic alterations. Here, using budding yeast, we identify a proteolytic pathway that contributes to alterations in mitochondrial structure in aged cells ...

journal_title：Molecular biology of the cell

authors： Goodrum JM,Lever AR,Coody TK,Gottschling DE,Hughes AL

更新日期：2019-08-01 00:00:00

abstract：:The Lec35 gene product (Lec35p) is required for utilization of the mannose donor mannose-P-dolichol (MPD) in synthesis of both lipid-linked oligosaccharides (LLOs) and glycosylphosphatidylinositols, which are important for functions such as protein folding and membrane anchoring, respectively. The hamster Lec35 gene i...

journal_title：Molecular biology of the cell

authors： Anand M,Rush JS,Ray S,Doucey MA,Weik J,Ware FE,Hofsteenge J,Waechter CJ,Lehrman MA

更新日期：2001-02-01 00:00:00

abstract：:We have identified a novel Ras-interacting protein from Dictyostelium, RIP3, whose function is required for both chemotaxis and the synthesis and relay of the cyclic AMP (cAMP) chemoattractant signal. rip3 null cells are unable to aggregate and lack receptor activation of adenylyl cyclase but are able, in response to ...

journal_title：Molecular biology of the cell

authors： Lee S,Parent CA,Insall R,Firtel RA

更新日期：1999-09-01 00:00:00

abstract：:A novel selection scheme has been developed to isolate bloodstream forms of Trypanosoma brucei, which are defective in their ability to differentiate to the procyclic stage. Detailed characterization of one selected cell line (defective in differentiation clone 1 [DiD-1]) has demonstrated that these cells are indistin...

journal_title：Molecular biology of the cell

authors： Tasker M,Wilson J,Sarkar M,Hendriks E,Matthews K

更新日期：2000-05-01 00:00:00

abstract：:Griscelli syndrome type 2 (GS2) is a genetic disorder in which patients exhibit life-threatening defects of cytotoxic T lymphocytes (CTLs) whose lytic granules fail to dock on the plasma membrane and therefore do not release their contents. The disease is caused by the absence of functional rab27a, but how rab27a cont...

journal_title：Molecular biology of the cell

authors： Neeft M,Wieffer M,de Jong AS,Negroiu G,Metz CH,van Loon A,Griffith J,Krijgsveld J,Wulffraat N,Koch H,Heck AJ,Brose N,Kleijmeer M,van der Sluijs P

更新日期：2005-02-01 00:00:00

abstract：:LLCPK-1 cells were transfected with a green fluorescent protein (GFP)-alpha tubulin construct and a cell line permanently expressing GFP-alpha tubulin was established (LLCPK-1alpha). The mitotic index and doubling time for LLCPK-1alpha were not significantly different from parental cells. Quantitative immunoblotting s...

journal_title：Molecular biology of the cell

authors： Rusan NM,Fagerstrom CJ,Yvon AM,Wadsworth P

更新日期：2001-04-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides d...

journal_title：Molecular biology of the cell

authors： Verbsky JW,Turkewitz AP

更新日期：1998-02-01 00:00:00

abstract：:Many of the actions of the neuropeptide substance P (SP) that are mediated by the neurokinin 1 receptor (NK1-R) desensitize and resensitize, which may be associated with NK1-R endocytosis and recycling. We delineated this endocytic pathway in transfected cells by confocal microscopy using cyanine 3-SP and NK1-R antibo...

journal_title：Molecular biology of the cell

authors： Grady EF,Garland AM,Gamp PD,Lovett M,Payan DG,Bunnett NW

更新日期：1995-05-01 00:00:00

abstract：:Activation of the NADPH oxidase of phagocytic cells requires the action of Rac2 or Rac1, members of the Ras superfamily of GTP-binding proteins. Rac proteins are active when in the GTP-bound form and can be regulated by a variety of proteins that modulate the exchange of GDP for GTP and/or GTP hydrolysis. The p190 Rac...

journal_title：Molecular biology of the cell

authors： Heyworth PG,Knaus UG,Settleman J,Curnutte JT,Bokoch GM

更新日期：1993-11-01 00:00:00