The proteomics of quiescent and nonquiescent cell differentiation in yeast stationary-phase cultures.

abstract：:The vast complexity of platelet-derived growth factor (PDGF)-induced downstream signaling pathways is well known, but the precise roles of critical players still elude us due to our lack of specific and temporal control over their activities. Accordingly, although Src family members are some of the better characterize...

journal_title：Molecular biology of the cell

authors： Shah K,Vincent F

更新日期：2005-11-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts wit...

journal_title：Molecular biology of the cell

authors： Newpher TM,Idrissi FZ,Geli MI,Lemmon SK

更新日期：2006-10-01 00:00:00

abstract：:Cotranslational protein targeting to membranes is regulated by two GTPases in the signal recognition particle (SRP) and the SRP receptor; association between the two GTPases is slow and is accelerated 400-fold by the SRP RNA. Intriguingly, the otherwise universally conserved SRP RNA is missing in a novel chloroplast S...

journal_title：Molecular biology of the cell

authors： Jaru-Ampornpan P,Chandrasekar S,Shan SO

更新日期：2007-07-01 00:00:00

abstract：:In Schizosaccharomyces pombe, the Sty1 mitogen-activated protein kinase and the Atf1 transcription factor control transcriptional induction in response to elevated salt concentrations. Herein, we demonstrate that two repressors, Tup11 and Tup12, and the Prr1 transcription factor also function in the response to salt s...

journal_title：Molecular biology of the cell

authors： Greenall A,Hadcroft AP,Malakasi P,Jones N,Morgan BA,Hoffman CS,Whitehall SK

更新日期：2002-09-01 00:00:00

abstract：:Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of i...

journal_title：Molecular biology of the cell

authors： Shekhar S,Carlier MF

更新日期：2016-01-01 00:00:00

abstract：:Functional proteins of complex eukaryotes within the same species are rather invariant. A single catalytic component of telomerase TERT is essential for an active telomerase complex that maintains telomeres. Surprisingly, we have identified two paralogous SpTERT-L and SpTERT-S genes with novel domains in Strongylocent...

journal_title：Molecular biology of the cell

authors： Wells TB,Zhang G,Harley Z,Vaziri H

更新日期：2009-01-01 00:00:00

abstract：:The nucleus houses, organizes, and protects chromatin to ensure genome integrity and proper gene expression, but how the nucleus adapts mechanically to changes in the extracellular environment is poorly understood. Recent studies have revealed that extracellular physical stresses induce chromatin compaction via mechan...

journal_title：Molecular biology of the cell

authors： Stephens AD,Liu PZ,Kandula V,Chen H,Almassalha LM,Herman C,Backman V,O'Halloran T,Adam SA,Goldman RD,Banigan EJ,Marko JF

更新日期：2019-06-19 00:00:00

abstract：:The engagement of integrin alpha7 in E63 skeletal muscle cells by laminin or anti-alpha7 antibodies triggered transient elevations in the intracellular free Ca(2+) concentration that resulted from both inositol triphosphate-evoked Ca(2+) release from intracellular stores and extracellular Ca(2+) influx through voltage...

journal_title：Molecular biology of the cell

authors： Kwon MS,Park CS,Choi K,Ahnn J,Kim JI,Eom SH,Kaufman SJ,Song WK

更新日期：2000-04-01 00:00:00

abstract：:Gaucher disease is a lysosomal storage disorder caused by a defect in the degradation of glucosylceramide catalyzed by the lysosomal enzyme β-glucocerebrosidase (GBA). GBA reaches lysosomes via association with its receptor, lysosomal integral membrane protein type 2 (LIMP-2). We found that distinct phosphatidylinosit...

journal_title：Molecular biology of the cell

authors： Jović M,Kean MJ,Szentpetery Z,Polevoy G,Gingras AC,Brill JA,Balla T

更新日期：2012-04-01 00:00:00

abstract：:Cdc2 kinase activity is required for triggering entry into mitosis in all known eukaryotes. Elaborate mechanisms have evolved for regulating Cdc2 activity so that mitosis occurs in a timely manner, when preparations for its execution are complete. In Schizosaccharomyces pombe, Wee1 and a related Mik1 kinase are Cdc2-i...

journal_title：Molecular biology of the cell

authors： Campbell SD,Sprenger F,Edgar BA,O'Farrell PH

更新日期：1995-10-01 00:00:00

abstract：:S100A4, a member of the S100 family of Ca(2+)-binding proteins, is directly involved in tumor metastasis. In addition to its expression in tumor cells, S100A4 is expressed in normal cells and tissues, including fibroblasts and cells of the immune system. To examine the contribution of S100A4 to normal physiology, we e...

journal_title：Molecular biology of the cell

authors： Li ZH,Dulyaninova NG,House RP,Almo SC,Bresnick AR

更新日期：2010-08-01 00:00:00

abstract：:Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Perrin BJ,Bennin DA,Huttenlocher A

更新日期：2010-01-01 00:00:00

abstract：:Methionine residues in proteins are susceptible to oxidation by reactive oxygen species, but can be repaired via reduction of the resulting methionine sulfoxides by methionine-S-sulfoxide reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB). However, the identity of all methionine sulfoxide reductases involved...

journal_title：Molecular biology of the cell

authors： Kim HY,Gladyshev VN

更新日期：2004-03-01 00:00:00

abstract：:EB1 proteins are ubiquitous microtubule-associated proteins involved in microtubule search and capture, regulation of microtubule dynamics, cell polarity, and chromosome stability. We have cloned a complete cDNA of Dictyostelium EB1 (DdEB1), the largest known EB1 homolog (57 kDa). Immunofluorescence analysis and expre...

journal_title：Molecular biology of the cell

authors： Rehberg M,Gräf R

更新日期：2002-07-01 00:00:00

abstract：:A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal ...

journal_title：Molecular biology of the cell

authors： Hut HM,Lemstra W,Blaauw EH,Van Cappellen GW,Kampinga HH,Sibon OC

更新日期：2003-05-01 00:00:00

abstract：:We have shown previously that the transforming growth factor-beta (TGFbeta)-regulated Sma-Mad (Smad) protein 3 and Smad4 proteins transactivate the apolipoprotein C-III promoter in hepatic cells via a hormone response element that binds the nuclear receptor hepatocyte nuclear factor 4 (HNF-4). In the present study, we...

journal_title：Molecular biology of the cell

authors： Chou WC,Prokova V,Shiraishi K,Valcourt U,Moustakas A,Hadzopoulou-Cladaras M,Zannis VI,Kardassis D

更新日期：2003-03-01 00:00:00

abstract：:The efficient activation of p90(rsk) by MAP kinase requires their interaction through a docking site located at the C-terminal end of p90(rsk). The MAP kinase p42(mpk1) can associate with p90(rsk) in G(2)-arrested but not in mature Xenopus oocytes. In contrast, an N-terminally truncated p90(rsk) mutant named D2 consti...

journal_title：Molecular biology of the cell

authors： Gavin AC,Ni Ainle A,Chierici E,Jones M,Nebreda AR

更新日期：1999-09-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:The Aurora kinase complex, also called the chromosomal passenger complex (CPC), is essential for faithful chromosome segregation and completion of cell division. In Fungi and Animalia, this complex consists of the kinase Aurora B/AIR-2/Ipl1p, INCENP/ICP-1/Sli15p, and Survivin/BIR-1/Bir1p. A fourth subunit, Borealin/Da...

journal_title：Molecular biology of the cell

authors： Nakajima Y,Tyers RG,Wong CC,Yates JR 3rd,Drubin DG,Barnes G

更新日期：2009-03-01 00:00:00

abstract：:The target of rapamycin kinase complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. In Saccharomyces cerevisiae, TORC1 activity is known to be controlled by the conserved GTPases, Gtr1/2, and movement into and out of an inactive agglomerate/body. However, it is unclear whether/how these regulatory ste...

journal_title：Molecular biology of the cell

authors： Sullivan A,Wallace RL,Wellington R,Luo X,Capaldi AP

更新日期：2019-02-01 00:00:00

abstract：:Myosin II motors embedded within the actin cortex generate contractile forces to modulate cell shape in essential behaviors, including polarization, migration, and division. In sarcomeres, myosin II-mediated sliding of antiparallel F-actin is tightly coupled to myofibril contraction. By contrast, cortical F-actin is h...

journal_title：Molecular biology of the cell

authors： Murrell M,Gardel ML

更新日期：2014-06-15 00:00:00

abstract：:When demembranated sperm nuclei are placed in a Xenopus egg extract, they become surrounded by a nuclear envelope and then swell to form morphologically typical pronuclei. Granules ranging from < 1.0 to approximately 3.0 microns in diameter appear within such nuclei. Bell et al. identified four nucleolar proteins in t...

journal_title：Molecular biology of the cell

authors： Bauer DW,Murphy C,Wu Z,Wu CH,Gall JG

更新日期：1994-06-01 00:00:00

abstract：:The development and progression of cardiac hypertrophy often leads to heart failure and death, and important modulators of hypertrophy include the histone deacetylase proteins (HDACs). Estrogen inhibits cardiac hypertrophy and progression in animal models and humans. We therefore investigated the influence of 17-β-est...

journal_title：Molecular biology of the cell

authors： Pedram A,Razandi M,Narayanan R,Dalton JT,McKinsey TA,Levin ER

更新日期：2013-12-01 00:00:00

abstract：:Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phas...

journal_title：Molecular biology of the cell

authors： Bodor DL,Valente LP,Mata JF,Black BE,Jansen LE

更新日期：2013-04-01 00:00:00

abstract：:Topogenic determinants that direct protein topology at the endoplasmic reticulum membrane usually function with high fidelity to establish a uniform topological orientation for any given polypeptide. Here we show, however, that through the coupling of sequential translocation events, native topogenic determinants are ...

journal_title：Molecular biology of the cell

authors： Moss K,Helm A,Lu Y,Bragin A,Skach WR

更新日期：1998-09-01 00:00:00

abstract：:When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins a...

journal_title：Molecular biology of the cell

authors： González-Novo A,Correa-Bordes J,Labrador L,Sánchez M,Vázquez de Aldana CR,Jiménez J

更新日期：2008-04-01 00:00:00

abstract：:Gp78 is an ERAD-associated E3 ubiquitin ligase that induces degradation of the mitofusin mitochondrial fusion proteins and mitochondrial fission. Gp78 is localized throughout the ER; however, the anti-Gp78 3F3A monoclonal antibody (mAb) recognizes Gp78 selectively in mitochondria-associated ER domains. Epitope mapping...

journal_title：Molecular biology of the cell

authors： Li L,Gao G,Shankar J,Joshi B,Foster LJ,Nabi IR

更新日期：2015-11-01 00:00:00

abstract：:The 260-kDa heterodimeric Gal/GalNAc-specific Lectin (Gal-lectin) of Entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kDa) and a light subunit (lgl, 35 kDa). We have previously shown that inhibition of expression of the 35-kDa subunit by antisense RNA causes a decrease in virulence. T...

journal_title：Molecular biology of the cell

authors： Katz U,Ankri S,Stolarsky T,Nuchamowitz Y,Mirelman D

更新日期：2002-12-01 00:00:00

abstract：:Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outst...

journal_title：Molecular biology of the cell

authors： Graef M,Friedman JR,Graham C,Babu M,Nunnari J

更新日期：2013-09-01 00:00:00

abstract：:The spindle checkpoint prevents anaphase onset until all the chromosomes have successfully attached to the spindle microtubules. The mechanisms by which unattached kinetochores trigger and transmit a primary signal are poorly understood, although it seems to be dependent at least in part, on the kinetochore localizati...

journal_title：Molecular biology of the cell

authors： Vigneron S,Prieto S,Bernis C,Labbé JC,Castro A,Lorca T

更新日期：2004-10-01 00:00:00