Actomyosin sliding is attenuated in contractile biomimetic cortices.

abstract：:Various human diseases are associated with mitochondrial DNA (mtDNA) mutations, but heteroplasmy—the coexistence of mutant and wild-type mtDNA—complicates their study. We previously isolated a temperature-lethal mtDNA mutation in Drosophila, mt:CoI(T300I), which affects the cytochrome c oxidase subunit I (CoI) locus. ...

journal_title：Molecular biology of the cell

authors： Chen Z,Qi Y,French S,Zhang G,Covian Garcia R,Balaban R,Xu H

更新日期：2015-02-15 00:00:00

abstract：:Golgi fragmentation is a common feature in multiple neurodegenerative diseases; however, the precise mechanism that causes fragmentation remains obscure. A potential link between Cdk5 and Golgi fragmentation in Alzheimer's disease (AD) was investigated in this study. Because Golgi is physiologically fragmented during ...

journal_title：Molecular biology of the cell

authors： Sun KH,de Pablo Y,Vincent F,Johnson EO,Chavers AK,Shah K

更新日期：2008-07-01 00:00:00

abstract：:Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

journal_title：Molecular biology of the cell

authors： Zhou D,Ursitti JA,Bloch RJ

更新日期：1998-01-01 00:00:00

abstract：:ARVCF, an armadillo-repeat protein of the p120(ctn) family, associates with classical cadherins and is present in adherens junctions, but its function is poorly understood. Here, we show that ARVCF interacts via a C-terminal PDZ-binding motif with zonula occludens (ZO)-1 and ZO-2. ARVCF and ZO-1 partially colocalize i...

journal_title：Molecular biology of the cell

authors： Kausalya PJ,Phua DC,Hunziker W

更新日期：2004-12-01 00:00:00

abstract：:My group and I feel tremendously honored to be recognized with the 2016 Early Career Life Scientist Award from the American Society for Cell Biology. In this essay I share the scientific questions that my lab has been excitedly pursuing since starting in August 2009 and the leadership behaviors we have adopted that en...

journal_title：Molecular biology of the cell

authors： Greco V

更新日期：2016-11-01 00:00:00

abstract：:Autocrine motility factor receptor (AMF-R) is a cell surface receptor that is also localized to a smooth subdomain of the endoplasmic reticulum, the AMF-R tubule. By postembedding immunoelectron microscopy, AMF-R concentrates within smooth plasmalemmal vesicles or caveolae in both NIH-3T3 fibroblasts and HeLa cells. B...

journal_title：Molecular biology of the cell

authors： Benlimame N,Le PU,Nabi IR

更新日期：1998-07-01 00:00:00

abstract：:The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

journal_title：Molecular biology of the cell

authors： Melvin A,Mudie S,Rocha S

更新日期：2011-11-01 00:00:00

abstract：:Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides d...

journal_title：Molecular biology of the cell

authors： Verbsky JW,Turkewitz AP

更新日期：1998-02-01 00:00:00

abstract：:Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblas...

journal_title：Molecular biology of the cell

authors： Laulagnier K,Schieber NL,Maritzen T,Haucke V,Parton RG,Gruenberg J

更新日期：2011-06-15 00:00:00

abstract：:Eukaryotic cells eliminate misfolded proteins from the endoplasmic reticulum (ER) via a conserved process termed ER-associated degradation (ERAD). Central regulators of the ERAD system are membrane-bound ubiquitin ligases, which are thought to channel misfolded proteins through the ER membrane during retrotranslocatio...

journal_title：Molecular biology of the cell

authors： Zhang T,Xu Y,Liu Y,Ye Y

更新日期：2015-12-01 00:00:00

abstract：:In primary hepatocytes and HepG2 hepatoma cells, prolonged activation of the p42/44 mitogen-activated protein kinase (MAPK) pathway is associated with a reduction in DNA synthesis, mediated by increased expression of the cyclin-dependent kinase inhibitor protein p21 (Cip-1/WAF1/mda6) (p21). This study was performed to...

journal_title：Molecular biology of the cell

authors： Park JS,Qiao L,Gilfor D,Yang MY,Hylemon PB,Benz C,Darlington G,Firestone G,Fisher PB,Dent P

更新日期：2000-09-01 00:00:00

abstract：:Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in these organelles is unknown. Here we report that Stx3 underg...

journal_title：Molecular biology of the cell

authors： Giovannone AJ,Reales E,Bhattaram P,Fraile-Ramos A,Weimbs T

更新日期：2017-10-15 00:00:00

abstract：:MARK2 regulates the establishment of polarity in Madin-Darby canine kidney (MDCK) cells in part through phosphorylation of serine 227 of Rab11-FIP2. We identified Eps15 as an interacting partner of phospho-S227-Rab11-FIP2 (pS227-FIP2). During recovery from low calcium, Eps15 localized to the lateral membrane before pS...

journal_title：Molecular biology of the cell

authors： Lapierre LA,Manning EH,Mitchell KM,Caldwell CM,Goldenring JR

更新日期：2017-04-15 00:00:00

abstract：:SLC45A2 encodes a putative transporter expressed primarily in pigment cells. SLC45A2 mutations cause oculocutaneous albinism type 4 (OCA4) and polymorphisms are associated with pigmentation variation, but the localization, function, and regulation of SLC45A2 and its variants remain unknown. We show that SLC45A2 locali...

journal_title：Molecular biology of the cell

authors： Le L,Escobar IE,Ho T,Lefkovith AJ,Latteri E,Haltaufderhyde KD,Dennis MK,Plowright L,Sviderskaya EV,Bennett DC,Oancea E,Marks MS

更新日期：2020-11-15 00:00:00

abstract：:In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuol...

journal_title：Molecular biology of the cell

authors： Kabeya Y,Kamada Y,Baba M,Takikawa H,Sasaki M,Ohsumi Y

更新日期：2005-05-01 00:00:00

abstract：:Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

journal_title：Molecular biology of the cell

authors： Bendezú FO,Martin SG

更新日期：2011-01-01 00:00:00

abstract：:TPX2 is a Ran-regulated spindle assembly factor that is required for kinetochore fiber formation and activation of the mitotic kinase Aurora A. TPX2 is enriched near spindle poles and is required near kinetochores, suggesting that it undergoes dynamic relocalization throughout mitosis. Using photoactivation, we measur...

journal_title：Molecular biology of the cell

authors： Ma N,Tulu US,Ferenz NP,Fagerstrom C,Wilde A,Wadsworth P

更新日期：2010-03-15 00:00:00

abstract：:Many biological systems respond to environmental changes by activating intracellular signaling cascades, resulting in an appropriate response. One such system is represented by the social amoeba Dictyostelium discoideum. When food sources become scarce, these unicellular cells can initiate a cAMP-driven multicellular ...

journal_title：Molecular biology of the cell

authors： Vinet AF,Fiedler T,Studer V,Froquet R,Dardel A,Cosson P,Pieters J

更新日期：2014-03-01 00:00:00

abstract：:Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane protein expressed on microglia within the brain. Several rare mutations in TREM2 cause an early-onset form of neurodegeneration when inherited homozygously. Here we investigate how these mutations affect the intracellular transport of TREM2. W...

journal_title：Molecular biology of the cell

authors： Sirkis DW,Aparicio RE,Schekman R

更新日期：2017-10-01 00:00:00

abstract：:The guanosine trisphosphatase Rap1 serves as a critical player in signal transduction, somatic cell proliferation and differentiation, and cell-cell adhesion by acting through distinct mechanisms. During mouse spermiogenesis, Rap1 is activated and forms a signaling complex with its effector, the serine-threonine kinas...

journal_title：Molecular biology of the cell

authors： Aivatiadou E,Mattei E,Ceriani M,Tilia L,Berruti G

更新日期：2007-04-01 00:00:00

abstract：:To determine how the human cdc25 gene product acts to regulate p34cdc2 at the G2 to M transition, we have overproduced the full-length protein (cdc25Hs) as well as several deletion mutants in bacteria as glutathione-S-transferase fusion proteins. The wild-type cdc25Hs gene product was synthesized as an 80-kDa fusion p...

journal_title：Molecular biology of the cell

authors： Lee MS,Ogg S,Xu M,Parker LL,Donoghue DJ,Maller JL,Piwnica-Worms H

更新日期：1992-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a leading cause of death worldwide. Using cDNA microarrays to characterize patterns of gene expression in HCC, we found consistent differences between the expression patterns in HCC compared with those seen in nontumor liver tissues. The expression patterns in HCC were also readily di...

journal_title：Molecular biology of the cell

authors： Chen X,Cheung ST,So S,Fan ST,Barry C,Higgins J,Lai KM,Ji J,Dudoit S,Ng IO,Van De Rijn M,Botstein D,Brown PO

更新日期：2002-06-01 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1) protects cells from oxidative stress via the base excision repair pathway and as a redox transcriptional coactivator. It is required for tumor progression/metastasis, and its up-regulation is associated with cancer resistance. Loss of APE1 expression causes ce...

journal_title：Molecular biology of the cell

authors： Vascotto C,Bisetto E,Li M,Zeef LA,D'Ambrosio C,Domenis R,Comelli M,Delneri D,Scaloni A,Altieri F,Mavelli I,Quadrifoglio F,Kelley MR,Tell G

更新日期：2011-10-01 00:00:00

abstract：:Toll-like receptor 4 interactor with leucine-rich repeats (Tril) functions as a coreceptor for Toll-like receptors (Tlrs) to mediate innate immune responses in adults. In embryos, Tril signals to promote degradation of the Bmp inhibitor, Smad7, to allow for blood formation. It is not known whether this function requir...

journal_title：Molecular biology of the cell

authors： Kim HS,McKnite A,Xie Y,Christian JL

更新日期：2018-03-01 00:00:00

abstract：:We screened a human cDNA library with a probe derived from a partial SEC23 mouse homologue and isolated two different cDNA clones (hSec23A and hSec23B) encoding proteins of a predicted molecular mass of 85 kDa. hSec23Ap and hSec23Bp were 85% identical and shared 48% identity with the yeast Sec23p. Affinity-purified an...

journal_title：Molecular biology of the cell

authors： Paccaud JP,Reith W,Carpentier JL,Ravazzola M,Amherdt M,Schekman R,Orci L

更新日期：1996-10-01 00:00:00

abstract：:Mutations in the mitochondrial cardiolipin (CL) transacylase, tafazzin (Taz1p), result in the X-linked cardioskeletal myopathy, Barth syndrome (BTHS). The mitochondria of BTHS patients exhibit variable respiratory defects and abnormal cristae ultrastructure. The biochemical basis for these observations is unknown. In ...

journal_title：Molecular biology of the cell

authors： Claypool SM,Boontheung P,McCaffery JM,Loo JA,Koehler CM

更新日期：2008-12-01 00:00:00

abstract：:The Drosophila melanogaster genes Hrb87F and Hrb98DE encode the fly proteins HRB87F and HRB98DE (also known as hrp36 and hrp38, respectively) that are most similar in sequence and function to mammalian A/B-type hnRNP proteins. Using overexpression and deletion mutants of Hrb87F, we have tested the hypothesis that the ...

journal_title：Molecular biology of the cell

authors： Zu K,Sikes ML,Haynes SR,Beyer AL

更新日期：1996-07-01 00:00:00

abstract：:The majority of Rab proteins are posttranslationally modified with two geranylgeranyl lipid moieties that enable their stable association with membranes. In this study, we present evidence to demonstrate that there is a specific lipid requirement for Rab protein localization and function. Substitution of different pre...

journal_title：Molecular biology of the cell

authors： Calero M,Chen CZ,Zhu W,Winand N,Havas KA,Gilbert PM,Burd CG,Collins RN

更新日期：2003-05-01 00:00:00

abstract：:Thrombospondin (TSP) was demonstrated to inhibit the growth of bovine aortic endothelial cells, an activity that was not neutralized by antibodies to TSP or by other agents that block TSP-cell interactions but that partially was reversed by a neutralizing antibody to transforming growth factor-beta (TGF-beta). Similar...

journal_title：Molecular biology of the cell

authors： Murphy-Ullrich JE,Schultz-Cherry S,Höök M

更新日期：1992-02-01 00:00:00

abstract：:Cdc42-associated tyrosine kinase 1 (ACK1) is a specific down-stream effector of Cdc42, a Rho family small G-protein. Previous studies have shown that ACK1 interacts with clathrin heavy chain and is involved in clathrin-coated vesicle endocytosis. Here we report that ACK1 interacted with epidermal growth factor recepto...

journal_title：Molecular biology of the cell

authors： Shen F,Lin Q,Gu Y,Childress C,Yang W

更新日期：2007-03-01 00:00:00