The thrill of scientific discovery and leadership with my group.

Abstract:

:My group and I feel tremendously honored to be recognized with the 2016 Early Career Life Scientist Award from the American Society for Cell Biology. In this essay I share the scientific questions that my lab has been excitedly pursuing since starting in August 2009 and the leadership behaviors we have adopted that enable our collective scientific productivity.

journal_name

Mol Biol Cell

authors

Greco V

doi

10.1091/mbc.E16-06-0373

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

3185-3188

issue

21

eissn

1059-1524

issn

1939-4586

pii

27/21/3185

journal_volume

27

pub_type

杂志文章
  • The tyrosine kinase activity of c-Src regulates actin dynamics and organization of podosomes in osteoclasts.

    abstract::Podosomes are dynamic actin-rich structures composed of a dense F-actin core surrounded by a cloud of more diffuse F-actin. Src performs one or more unique functions in osteoclasts (OCLs), and podosome belts and bone resorption are impaired in the absence of Src. Using Src(-/-) OCLs, we investigated the specific funct...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e07-03-0227

    authors: Destaing O,Sanjay A,Itzstein C,Horne WC,Toomre D,De Camilli P,Baron R

    更新日期:2008-01-01 00:00:00

  • Phosphatidylinositol-(4,5)-bisphosphate regulates clathrin-coated pit initiation, stabilization, and size.

    abstract::Clathrin-mediated endocytosis (CME) is the major mechanism for internalization in mammalian cells. CME initiates by recruitment of adaptors and clathrin to form clathrin-coated pits (CCPs). Nearly half of nascent CCPs abort, whereas others are stabilized by unknown mechanisms and undergo further maturation before pinc...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E11-04-0362

    authors: Antonescu CN,Aguet F,Danuser G,Schmid SL

    更新日期:2011-07-15 00:00:00

  • A Rnd3/p190RhoGAP pathway regulates RhoA activity in idiopathic pulmonary fibrosis fibroblasts.

    abstract::Idiopathic pulmonary fibrosis (IPF) is an incurable disease of the lung that is characterized by excessive deposition of extracellular matrix (ECM), resulting in disruption of normal lung function. The signals regulating fibrosis include both transforming growth factor beta (TGF-β) and tissue rigidity and a major sign...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-11-0642

    authors: Monaghan-Benson E,Wittchen ES,Doerschuk CM,Burridge K

    更新日期:2018-09-01 00:00:00

  • Early stages of the secretory pathway, but not endosomes, are required for Cvt vesicle and autophagosome assembly in Saccharomyces cerevisiae.

    abstract::The Cvt pathway is a biosynthetic transport route for a distinct subset of resident yeast vacuolar hydrolases, whereas macroautophagy is a nonspecific degradative mechanism that allows cell survival during starvation. Yet, these two vacuolar trafficking pathways share a number of identical molecular components and are...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e03-07-0479

    authors: Reggiori F,Wang CW,Nair U,Shintani T,Abeliovich H,Klionsky DJ

    更新日期:2004-05-01 00:00:00

  • Myosin IIC: a third molecular motor driving neuronal dynamics.

    abstract::Neuronal dynamics result from the integration of forces developed by molecular motors, especially conventional myosins. Myosin IIC is a recently discovered nonsarcomeric conventional myosin motor, the function of which is poorly understood, particularly in relation to the separate but coupled activities of its close h...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e07-08-0744

    authors: Wylie SR,Chantler PD

    更新日期:2008-09-01 00:00:00

  • Phosphorylation and internalization of gp130 occur after IL-6 activation of Jak2 kinase in hepatocytes.

    abstract::Recent evidence has shown that members of the Jak kinase family are activated after IL-6 binds to its receptor complex, leading to a tyrosine phosphorylation of gp130, the IL-6 signal-transducing subunit. The different members of the IL-6 cytokine subfamily induce distinct patterns of Jak-Tyk phosphorylation in differ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.5.7.819

    authors: Wang Y,Fuller GM

    更新日期:1994-07-01 00:00:00

  • ER-golgi traffic is a prerequisite for efficient ER degradation.

    abstract::Protein quality control is an essential function of the endoplasmic reticulum. Misfolded proteins unable to acquire their native conformation are retained in the endoplasmic reticulum, retro-translocated back into the cytosol, and degraded via the ubiquitin-proteasome system. We show that efficient degradation of solu...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.01-08-0399

    authors: Taxis C,Vogel F,Wolf DH

    更新日期:2002-06-01 00:00:00

  • Regulation of endocytosis, nuclear translocation, and signaling of fibroblast growth factor receptor 1 by E-cadherin.

    abstract::Fibroblast growth factor (FGF) receptors (FGFRs) signal to modulate diverse cellular functions, including epithelial cell morphogenesis. In epithelial cells, E-cadherin plays a key role in cell-cell adhesion, and its function can be regulated through endocytic trafficking. In this study, we investigated the location, ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e04-09-0845

    authors: Bryant DM,Wylie FG,Stow JL

    更新日期:2005-01-01 00:00:00

  • The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH.

    abstract::The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E11-02-0163

    authors: Melvin A,Mudie S,Rocha S

    更新日期:2011-11-01 00:00:00

  • TGF beta-induced growth inhibition in primary fibroblasts requires the retinoblastoma protein.

    abstract::Transforming growth factor beta (TGF beta) inhibits cell proliferation by inducing a G1 cell-cycle arrest. Cyclin/CDK complexes have been implicated in this arrest, because TGF beta treatment leads to inhibition of cyclin/CDK activity. We have investigated the role of the retinoblastoma protein (pRb) in TGF beta-induc...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.7.9.1335

    authors: Herrera RE,Mäkelä TP,Weinberg RA

    更新日期:1996-09-01 00:00:00

  • Compartmentalized cyclic adenosine 3',5'-monophosphate at the plasma membrane clusters PDE3A and cystic fibrosis transmembrane conductance regulator into microdomains.

    abstract::Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrate that phosphodiesterase type 3A (PDE3A) physically and functionally interacts with cystic fibrosis transmembrane conductance regula...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e09-08-0655

    authors: Penmatsa H,Zhang W,Yarlagadda S,Li C,Conoley VG,Yue J,Bahouth SW,Buddington RK,Zhang G,Nelson DJ,Sonecha MD,Manganiello V,Wine JJ,Naren AP

    更新日期:2010-03-15 00:00:00

  • Phosphatidic acid induces ligand-independent epidermal growth factor receptor endocytic traffic through PDE4 activation.

    abstract::Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E10-02-0167

    authors: Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

    更新日期:2010-08-15 00:00:00

  • Glycoprotein gp130 of dictyostelium discoideum influences macropinocytosis and adhesion.

    abstract::Glycoprotein gp130, found on the plasma membrane of Dictyostelium discoideum amoebae, was postulated previously to play a role in phagocytosis. The gene for gp130 was cloned and when translated, yielded a 768 amino acid preproprotein of 85.3 kDa. It had nearly 40% similarity to the 138 kDa family of glycoproteins impl...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e04-06-0483

    authors: Chia CP,Gomathinayagam S,Schmaltz RJ,Smoyer LK

    更新日期:2005-06-01 00:00:00

  • The Saccharomyces cerevisiae spindle pole body is a dynamic structure.

    abstract::During spindle pole body (SPB) duplication, the new SPB is assembled at a distinct site adjacent to the old SPB. Using quantitative fluorescence methods, we studied the assembly and dynamics of the core structural SPB component Spc110p. The SPB core exhibits both exchange and growth in a cell cycle-dependent manner. D...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-10-0655

    authors: Yoder TJ,Pearson CG,Bloom K,Davis TN

    更新日期:2003-08-01 00:00:00

  • Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms.

    abstract::Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E18-06-0355

    authors: Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

    更新日期:2019-05-01 00:00:00

  • Gαq-mediated calcium dynamics and membrane tension modulate neurite plasticity.

    abstract::The formation and disruption of synaptic connections during development are a fundamental step in neural circuit formation. Subneuronal structures such as neurites are known to be sensitive to the level of spontaneous neuronal activity, but the specifics of how neurotransmitter-induced calcium activity regulates neuri...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E19-09-0536

    authors: Pearce KM,Bell M,Linthicum WH,Wen Q,Srinivasan J,Rangamani P,Scarlata S

    更新日期:2020-03-19 00:00:00

  • An aspartyl cathepsin, CTH3, is essential for proprotein processing during secretory granule maturation in Tetrahymena thermophila.

    abstract::In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E14-03-0833

    authors: Kumar S,Briguglio JS,Turkewitz AP

    更新日期:2014-08-15 00:00:00

  • Mechanistic insights into actin-driven polarity site movement in yeast.

    abstract::Directed cell growth or migration are critical for the development and function of many eukaryotic cells. These cells develop a dynamic "front" (also called "polarity site") that can change direction. Polarity establishment involves autocatalytic accumulation of polarity regulators, including the conserved Rho-family ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E20-01-0040

    authors: Ghose D,Lew D

    更新日期:2020-05-01 00:00:00

  • Ran1 functions to control the Cdc10/Sct1 complex through Puc1.

    abstract::We have undertaken a biochemical analysis of the regulation of the G1/S-phase transition and commitment to the cell cycle in the fission yeast Schizosaccharomyces pombe. The execution of Start requires the activity of the Cdc2 protein kinase and the Sct1/Cdc10 transcription complex. Progression through G1 also require...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.8.6.1117

    authors: Caligiuri M,Connolly T,Beach D

    更新日期:1997-06-01 00:00:00

  • Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.

    abstract::Chironomus tentans-repressor splicing factor (Ct-RSF) represses the activation of splicing by SR proteins in vitro. Ct-RSF colocalizes with the Ser-Arg-rich (SR) protein hrp45 in interchromatin granule clusters and coimmunoprecipitates with hrp45 in nuclear extracts. Ct-RSF and hrp45 can also interact directly in vitr...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e05-04-0339

    authors: Björk P,Wetterberg-Strandh I,Baurén G,Wieslander L

    更新日期:2006-01-01 00:00:00

  • Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast.

    abstract::We have shown that protein kinase A phosphorylation of t-SNAREs inhibits SNARE assembly and suppresses endo- and exocytosis in yeast. Herein, we show that protein kinase A phosphorylation of the Sso exocytic t-SNAREs promotes the binding of Vsm1, a potential SNARE regulator identified previously in our laboratory. Pho...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-12-0804

    authors: Marash M,Gerst JE

    更新日期:2003-08-01 00:00:00

  • Down-regulation of the Wnt/β-catenin signaling pathway by Cacnb4.

    abstract::The β4 isoform of the β-subunits of voltage-gated calcium channel regulates cell proliferation and cell cycle progression. Herein we show that coexpression of the β4-subunit with actors of the canonical Wnt/β-catenin signaling pathway in a hepatoma cell line inhibits Wnt-responsive gene transcription and decreases cel...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E17-01-0076

    authors: Rima M,Daghsni M,Lopez A,Fajloun Z,Lefrancois L,Dunach M,Mori Y,Merle P,Brusés JL,De Waard M,Ronjat M

    更新日期:2017-12-01 00:00:00

  • E-cadherin adhesion activates c-Src signaling at cell-cell contacts.

    abstract::Cadherin-based cell-cell contacts are prominent sites for phosphotyrosine signaling, being enriched in tyrosine-phosphorylated proteins and tyrosine kinases and phosphatases. The functional interplay between cadherin adhesion and tyrosine kinase signaling, however, is complex and incompletely understood. In this repor...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e06-12-1154

    authors: McLachlan RW,Kraemer A,Helwani FM,Kovacs EM,Yap AS

    更新日期:2007-08-01 00:00:00

  • PTC1 is required for vacuole inheritance and promotes the association of the myosin-V vacuole-specific receptor complex.

    abstract::Organelle inheritance occurs during cell division. In Saccharomyces cerevisiae, inheritance of the vacuole, and the distribution of mitochondria and cortical endoplasmic reticulum are regulated by Ptc1p, a type 2C protein phosphatase. Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved b...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e08-09-0954

    authors: Jin Y,Taylor Eves P,Tang F,Weisman LS

    更新日期:2009-03-01 00:00:00

  • Sequestration of cholesterol within the host late endocytic pathway restricts liver-stage Plasmodium development.

    abstract::While lysosomes are degradative compartments and one of the defenses against invading pathogens, they are also hubs of metabolic activity. Late endocytic compartments accumulate around Plasmodium berghei liver-stage parasites during development, and whether this is a host defense strategy or active recruitment by the ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E16-07-0531

    authors: Petersen W,Stenzel W,Silvie O,Blanz J,Saftig P,Matuschewski K,Ingmundson A

    更新日期:2017-03-15 00:00:00

  • The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus.

    abstract::The formation and maintenance of the bipolar mitotic spindle apparatus require a complex and balanced interplay of several mechanisms, including the stabilization and separation of polar microtubules and the action of various microtubule motors. Nonmicrotubule elements are also present throughout the spindle apparatus...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.3.11.1259

    authors: Yang CH,Snyder M

    更新日期:1992-11-01 00:00:00

  • Nse1-dependent recruitment of Smc5/6 to lesion-containing loci contributes to the repair defects of mutant complexes.

    abstract::Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.E11-03-0272

    authors: Tapia-Alveal C,O'Connell MJ

    更新日期:2011-12-01 00:00:00

  • Significance of 14-3-3 self-dimerization for phosphorylation-dependent target binding.

    abstract::14-3-3 proteins via binding serine/threonine-phosphorylated proteins regulate diverse intracellular processes in all eukaryotic organisms. Here, we examine the role of 14-3-3 self-dimerization in target binding, and in the susceptibility of 14-3-3 to undergo phosphorylation. Using a phospho-specific antibody developed...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.e02-12-0821

    authors: Shen YH,Godlewski J,Bronisz A,Zhu J,Comb MJ,Avruch J,Tzivion G

    更新日期:2003-11-01 00:00:00

  • Yeast exocytic v-SNAREs confer endocytosis.

    abstract::In yeast, homologues of the synaptobrevin/VAMP family of v-SNAREs (Snc1 and Snc2) confer the docking and fusion of secretory vesicles at the cell surface. As no v-SNARE has been shown to confer endocytosis, we examined whether yeast lacking the SNC genes, or possessing a temperature-sensitive allele of SNC1 (SNC1(ala4...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.11.10.3629

    authors: Gurunathan S,Chapman-Shimshoni D,Trajkovic S,Gerst JE

    更新日期:2000-10-01 00:00:00

  • Targeting of a tail-anchored protein to endoplasmic reticulum and mitochondrial outer membrane by independent but competing pathways.

    abstract::Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins posttranslationally select their target, but C-terminal charged residues play an important role. To investigate how discrimination b...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章

    doi:10.1091/mbc.12.8.2482

    authors: Borgese N,Gazzoni I,Barberi M,Colombo S,Pedrazzini E

    更新日期:2001-08-01 00:00:00