Abstract:
:Attack by the host powdery mildew Erysiphe cichoracearum usually results in successful penetration and rapid proliferation of the fungus on Arabidopsis. By contrast, the nonhost barley powdery mildew Blumeria graminis f. sp. hordei (Bgh) typically fails to penetrate Arabidopsis epidermal cells. In both instances the plant secretes cell wall appositions or papillae beneath the penetration peg of the fungus. Genetic screens for mutations that result in increased penetration of Bgh on Arabidopsis have recently identified the PEN1 syntaxin. Here we examine the role of PEN1 and of its closest homologue, SYP122, identified as a syntaxin whose expression is responsive to infection. pen1 syp122 double mutants are both dwarfed and necrotic, suggesting that the two syntaxins have overlapping functions. Although syp122-1 and the cell wall mur mutants have considerably more pronounced primary cell wall defects than pen1 mutants, these have relatively subtle or no effects on penetration resistance. Upon fungal attack, PEN1 appears to be actively recruited to papillae, and there is a 2-h delay in papillae formation in the pen1-1 mutant. We conclude that SYP122 may have a general function in secretion, including a role in cell wall deposition. By contrast, PEN1 appears to have a basal function in secretion and a specialized defense-related function, being required for the polarized secretion events that give rise to papilla formation.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Assaad FF,Qiu JL,Youngs H,Ehrhardt D,Zimmerli L,Kalde M,Wanner G,Peck SC,Edwards H,Ramonell K,Somerville CR,Thordal-Christensen Hdoi
10.1091/mbc.e04-02-0140keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
5118-29issue
11eissn
1059-1524issn
1939-4586pii
E04-02-0140journal_volume
15pub_type
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