Diacylglycerol kinase ζ regulates RhoA activation via a kinase-independent scaffolding mechanism.

abstract：:Cellular levels of the versatile second messenger cyclic (c)AMP are regulated by the antagonistic actions of the canonical G protein → adenylyl cyclase pathway that is initiated by G-protein-coupled receptors (GPCRs) and attenuated by phosphodiesterases (PDEs). Dysregulated cAMP signaling drives many diseases; for exa...

journal_title：Molecular biology of the cell

authors： Getz M,Swanson L,Sahoo D,Ghosh P,Rangamani P

更新日期：2019-06-15 00:00:00

abstract：:Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

journal_title：Molecular biology of the cell

authors： Klionsky DJ

更新日期：2016-03-01 00:00:00

abstract：:NSF and p97 are related AAA proteins implicated in membrane trafficking and organelle biogenesis. p97 is also involved in pathways that lead to ubiquitin-dependent proteolysis, including ER-associated degradation (ERAD). In this study, we have used dominant interfering ATP-hydrolysis deficient mutants (NSF(E329Q) and ...

journal_title：Molecular biology of the cell

authors： Dalal S,Rosser MF,Cyr DM,Hanson PI

更新日期：2004-02-01 00:00:00

abstract：:Preventing polyspermy during animal fertilization relies on modifications to the egg's extracellular matrix. On fertilization in sea urchins, the contents of cortical granules are secreted and rapidly assemble into the egg's extracellular vitelline layer, forming the fertilization envelope, a proteinaceous structure t...

journal_title：Molecular biology of the cell

authors： Wong JL,Wessel GM

更新日期：2006-12-01 00:00:00

abstract：:The small GTPase Ran is essential for spindle assembly. Ran is proposed to act through its nuclear import receptors importin alpha and/or importin beta to control the sequestration of proteins necessary for spindle assembly. To date, the molecular mechanisms by which the Ran pathway functions remain unclear. Using pur...

journal_title：Molecular biology of the cell

authors： Ems-McClung SC,Zheng Y,Walczak CE

更新日期：2004-01-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:Surface charges at the inner leaflet of the plasma membrane may contribute to regulate the surface recruitment of key signaling factors. Phosphatidylserine (PS) is an abundant charged lipid that may regulate charge distribution in different cell types. Here we characterize the subcellular distribution and function of ...

journal_title：Molecular biology of the cell

authors： Haupt A,Minc N

更新日期：2017-01-01 00:00:00

abstract：:As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies, and worms, choanoflagellates have been refractory to DNA delivery methods for expressing foreign...

journal_title：Molecular biology of the cell

authors： Booth DS,Szmidt-Middleton H,King N

更新日期：2018-12-01 00:00:00

abstract：:Cholesterol transport is an essential process in all multicellular organisms. In this study we applied two recently developed approaches to investigate the distribution and molecular mechanisms of cholesterol transport in Caenorhabditis elegans. The distribution of cholesterol in living worms was studied by imaging it...

journal_title：Molecular biology of the cell

authors： Matyash V,Geier C,Henske A,Mukherjee S,Hirsh D,Thiele C,Grant B,Maxfield FR,Kurzchalia TV

更新日期：2001-06-01 00:00:00

abstract：:The transcription factor Sp1 is ubiquitously expressed in different cells and thereby regulates the expression of genes involved in many cellular processes. This study reveals that Sp1 was phosphorylated during the mitotic stage in three epithelial tumor cell lines and one glioma cell line. By using different kinase i...

journal_title：Molecular biology of the cell

authors： Chuang JY,Wang YT,Yeh SH,Liu YW,Chang WC,Hung JJ

更新日期：2008-03-01 00:00:00

abstract：:Rods and rings (RRs) are large linear- or circular-shaped structures typically described as polymers of IMPDH (inosine monophosphate dehydrogenase). They have been observed across a wide variety of cell types and species and can be induced to form by inhibitors of IMPDH. RRs are thought to play a role in the regulatio...

journal_title：Molecular biology of the cell

authors： Schiavon CR,Griffin ME,Pirozzi M,Parashuraman R,Zhou W,Jinnah HA,Reines D,Kahn RA

更新日期：2018-09-15 00:00:00

abstract：:Op18/stathmin (Op18) is a phosphorylation-regulated microtubule destabilizer that is frequently overexpressed in tumors. The importance of Op18 in malignancy was recently suggested by identification of a somatic Q18-->E mutation of Op18 in an adenocarcinoma. We addressed the functional consequences of aberrant Op18 ex...

journal_title：Molecular biology of the cell

authors： Holmfeldt P,Brännström K,Stenmark S,Gullberg M

更新日期：2006-07-01 00:00:00

abstract：:Cilia and flagella are structurally and functionally conserved organelles present in basal as well as higher eukaryotes. The assembly of cilia requires a microtubule based scaffold called a basal body. The ninefold symmetry characteristic of basal bodies and the structurally similar centriole is organized around a hub...

journal_title：Molecular biology of the cell

authors： Culver BP,Meehl JB,Giddings TH Jr,Winey M

更新日期：2009-03-01 00:00:00

abstract：:Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a gen...

journal_title：Molecular biology of the cell

authors： Ródenas E,González-Aguilera C,Ayuso C,Askjaer P

更新日期：2012-03-01 00:00:00

abstract：:Pro-alpha-factor (pro-alphaf) is posttranslationally modified in the yeast Golgi complex by the addition of alpha1,6-, alpha1,2-, and alpha1,3-linked mannose to N-linked oligosaccharides and by a Kex2p-initiated proteolytic processing event. Previous work has indicated that the alpha1,6- and alpha1,3-mannosylation and...

journal_title：Molecular biology of the cell

authors： Brigance WT,Barlowe C,Graham TR

更新日期：2000-01-01 00:00:00

abstract：:The phenotypic switch of cardiac fibroblasts (CFs) to myofibroblasts is essential for normal and pathological wound healing. Relative hyperoxic challenge during reoxygenation causes myocardial remodeling. Here, we sought to characterize the novel O(2)-sensitive molecular mechanisms responsible for triggering the diffe...

journal_title：Molecular biology of the cell

authors： Roy S,Khanna S,Rink T,Radtke J,Williams WT,Biswas S,Schnitt R,Strauch AR,Sen CK

更新日期：2007-12-01 00:00:00

abstract：:Microtubules (MTs) in newt mitotic spindles grow faster than MTs in the interphase cytoplasmic microtubule complex (CMTC), yet spindle MTs do not have the long lengths or lifetimes of the CMTC microtubules. Because MTs undergo dynamic instability, it is likely that changes in the durations of growth or shortening are ...

journal_title：Molecular biology of the cell

authors： Gliksman NR,Skibbens RV,Salmon ED

更新日期：1993-10-01 00:00:00

abstract：:A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in...

journal_title：Molecular biology of the cell

authors： Jantsch V,Tang L,Pasierbek P,Penkner A,Nayak S,Baudrimont A,Schedl T,Gartner A,Loidl J

更新日期：2007-12-01 00:00:00

abstract：:The mitochondrial Hsp70 chaperone Ssq1 plays a dedicated role in the maturation of iron-sulfur (Fe/S) proteins, an essential process of mitochondria. Similar to its bacterial orthologue HscA, Ssq1 binds to the scaffold protein Isu1, thereby facilitating dissociation of the newly synthesized Fe/S cluster on Isu1 and it...

journal_title：Molecular biology of the cell

authors： Uzarska MA,Dutkiewicz R,Freibert SA,Lill R,Mühlenhoff U

更新日期：2013-06-01 00:00:00

abstract：:Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phas...

journal_title：Molecular biology of the cell

authors： Bodor DL,Valente LP,Mata JF,Black BE,Jansen LE

更新日期：2013-04-01 00:00:00

abstract：:Immunolocalization studies in epithelial cells revealed myo6 was associated with peripherally located vesicles that contained the transferrin receptor. Pulse-chase experiments after transferrin uptake showed that these vesicles were newly uncoated endocytic vesicles and that myo6 was recruited to these vesicles immedi...

journal_title：Molecular biology of the cell

authors： Aschenbrenner L,Lee T,Hasson T

更新日期：2003-07-01 00:00:00

abstract：:The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein,...

journal_title：Molecular biology of the cell

authors： Nagy G,Milosevic I,Mohrmann R,Wiederhold K,Walter AM,Sørensen JB

更新日期：2008-09-01 00:00:00

abstract：:Tubulogenesis by epithelial cells regulates kidney, lung, and mammary development, whereas that by endothelial cells regulates vascular development. Although functionally dissimilar, the processes necessary for tubulation by epithelial and endothelial cells are very similar. We performed microarray analysis to further...

journal_title：Molecular biology of the cell

authors： Albig AR,Schiemann WP

更新日期：2005-02-01 00:00:00

abstract：:Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

journal_title：Molecular biology of the cell

authors： Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

更新日期：2014-09-15 00:00:00

abstract：:The TOR kinases are conserved negative regulators of autophagy in response to nutrient conditions, but the signaling mechanisms are poorly understood. Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila. We s...

journal_title：Molecular biology of the cell

authors： Chang YY,Neufeld TP

更新日期：2009-04-01 00:00:00

abstract：:We recently identified transmembrane protein shrew-1 and showed that it is able to target to adherens junctions in polarized epithelial cells. This suggested shrew-1 possesses specific basolateral sorting motifs, which we analyzed by mutational analysis. Systematic mutation of amino acids in putative sorting signals i...

journal_title：Molecular biology of the cell

authors： Jakob V,Schreiner A,Tikkanen R,Starzinski-Powitz A

更新日期：2006-08-01 00:00:00

abstract：:In response to stress conditions (such as nutrient limitation or accumulation of damaged organelles) and certain pathological situations, eukaryotic cells use autophagy as a survival mechanism. During nutrient stress the main purpose of autophagy is to degrade cytoplasmic materials within the lysosome/vacuole lumen an...

journal_title：Molecular biology of the cell

authors： Yang Z,Huang J,Geng J,Nair U,Klionsky DJ

更新日期：2006-12-01 00:00:00

abstract：:After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing vacuoles (SCVs) undergo a maturation process that involves sequential acquisition of Rab5 and Rab7 and displacement toward the ...

journal_title：Molecular biology of the cell

authors： Harrison RE,Brumell JH,Khandani A,Bucci C,Scott CC,Jiang X,Finlay BB,Grinstein S

更新日期：2004-07-01 00:00:00

abstract：:Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to maintain a stable metap...

journal_title：Molecular biology of the cell

authors： Verdaasdonk JS,Gardner R,Stephens AD,Yeh E,Bloom K

更新日期：2012-07-01 00:00:00

abstract：:Focal adhesions are intricate protein complexes that facilitate cell attachment, migration, and cellular communication. Lasp-2 (LIM-nebulette), a member of the nebulin family of actin-binding proteins, is a newly identified component of these complexes. To gain further insights into the functional role of lasp-2, we i...

journal_title：Molecular biology of the cell

authors： Bliss KT,Chu M,Jones-Weinert CM,Gregorio CC

更新日期：2013-04-01 00:00:00