An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation.

abstract：:Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are...

journal_title：Molecular biology of the cell

authors： Gutiérrez-Escribano P,González-Novo A,Suárez MB,Li CR,Wang Y,de Aldana CR,Correa-Bordes J

更新日期：2011-07-15 00:00:00

abstract：:Mechanosensing describes the ability of a cell to sense mechanical cues of its microenvironment, including not only all components of force, stress, and strain but also substrate rigidity, topology, and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the ch...

journal_title：Molecular biology of the cell

authors： Chen Y,Ju L,Rushdi M,Ge C,Zhu C

更新日期：2017-11-07 00:00:00

abstract：:Gp78 is an ERAD-associated E3 ubiquitin ligase that induces degradation of the mitofusin mitochondrial fusion proteins and mitochondrial fission. Gp78 is localized throughout the ER; however, the anti-Gp78 3F3A monoclonal antibody (mAb) recognizes Gp78 selectively in mitochondria-associated ER domains. Epitope mapping...

journal_title：Molecular biology of the cell

authors： Li L,Gao G,Shankar J,Joshi B,Foster LJ,Nabi IR

更新日期：2015-11-01 00:00:00

abstract：:In autosomal dominant polycystic kidney disease (ADPKD), cysts accumulate and progressively impair renal function. Mutations in PKD1 and PKD2 genes are causally linked to ADPKD, but how these mutations drive cell behaviors that underlie ADPKD pathogenesis is unknown. Human ADPKD cysts frequently express cadherin-8 (ca...

journal_title：Molecular biology of the cell

authors： Belmonte JM,Clendenon SG,Oliveira GM,Swat MH,Greene EV,Jeyaraman S,Glazier JA,Bacallao RL

更新日期：2016-11-07 00:00:00

abstract：:Eukaryotic cells eliminate misfolded proteins from the endoplasmic reticulum (ER) via a conserved process termed ER-associated degradation (ERAD). Central regulators of the ERAD system are membrane-bound ubiquitin ligases, which are thought to channel misfolded proteins through the ER membrane during retrotranslocatio...

journal_title：Molecular biology of the cell

authors： Zhang T,Xu Y,Liu Y,Ye Y

更新日期：2015-12-01 00:00:00

abstract：:Silica inhalation leads to the development of the chronic lung disease silicosis. Macrophages are killed by uptake of nonopsonized silica particles, and this is believed to play a critical role in the etiology of silicosis. However, the mechanism of nonopsonized-particle uptake is not well understood. We compared the ...

journal_title：Molecular biology of the cell

authors： Gilberti RM,Knecht DA

更新日期：2015-02-01 00:00:00

abstract：:Ca2+-triggered exocytosis of synaptic vesicles is controlled by the Ca2+-binding protein synaptotagmin (syt) I. Fifteen additional isoforms of syt have been identified. Here, we compared the abilities of three syt isoforms (I, VII, and IX) to regulate soluble N-ethylmaleimide-sensitive factor attachment protein recept...

journal_title：Molecular biology of the cell

authors： Bhalla A,Tucker WC,Chapman ER

更新日期：2005-10-01 00:00:00

abstract：:Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle- and cell adhesion-related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morphol...

journal_title：Molecular biology of the cell

authors： Kerosuo L,Bronner ME

更新日期：2014-02-01 00:00:00

abstract：:The cytoskeleton plays an important role in neuronal morphogenesis. We have identified and characterized a novel actin-binding protein, termed Mayven, predominantly expressed in brain. Mayven contains a BTB (broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger) domain-like structure in the predicted N ter...

journal_title：Molecular biology of the cell

authors： Soltysik-Espanola M,Rogers RA,Jiang S,Kim TA,Gaedigk R,White RA,Avraham H,Avraham S

更新日期：1999-07-01 00:00:00

abstract：:Transporting epithelial cells optimize their morphology for solute uptake by building an apical specialization: a dense array of microvilli that serves to increase membrane surface area. In the intestinal tract, individual cells build thousands of microvilli, which pack tightly to form the brush border. Recent studies...

journal_title：Molecular biology of the cell

authors： Pinette JA,Mao S,Millis BA,Krystofiak ES,Faust JJ,Tyska MJ

更新日期：2019-01-01 00:00:00

abstract：:Members of the adenine nucleotide translocase (ANT) family exchange ADP for ATP across the mitochondrial inner membrane, an activity that is essential for oxidative phosphorylation (OXPHOS). Mutations in or dysregulation of ANTs is associated with progressive external ophthalmoplegia, cardiomyopathy, nonsyndromic inte...

journal_title：Molecular biology of the cell

authors： Lu YW,Acoba MG,Selvaraju K,Huang TC,Nirujogi RS,Sathe G,Pandey A,Claypool SM

更新日期：2017-06-01 00:00:00

abstract：:Heterotrimeric G proteins are components of principal signaling pathways in eukaryotes. In higher organisms, alpha subunits of G proteins have been divided into four families, Gi, Gs, Gq, and G12. We previously identified a G alpha i homologue gna-1 in the filamentous fungus Neurospora crassa. Now we report that delet...

journal_title：Molecular biology of the cell

authors： Ivey FD,Hodge PN,Turner GE,Borkovich KA

更新日期：1996-08-01 00:00:00

abstract：:Macrophage fusion resulting in the formation of multinucleated giant cells occurs in a variety of chronic inflammatory diseases, yet the mechanism responsible for initiating this process is unknown. Here, we used live cell imaging to show that actin-based protrusions at the leading edge initiate macrophage fusion. Pha...

journal_title：Molecular biology of the cell

authors： Faust JJ,Balabiyev A,Heddleston JM,Podolnikova NP,Baluch DP,Chew TL,Ugarova TP

更新日期：2019-08-01 00:00:00

abstract：:Treacher Collins syndrome (TCS) is an autosomal dominant disorder of craniofacial development caused by mutations in the gene TCOF1. Its gene product, treacle, consists mainly of a central repeat domain, which shows it to be structurally related to the nucleolar phosphoprotein Nopp140. Treacle remains mostly uncharact...

journal_title：Molecular biology of the cell

authors： Isaac C,Marsh KL,Paznekas WA,Dixon J,Dixon MJ,Jabs EW,Meier UT

更新日期：2000-09-01 00:00:00

abstract：:The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at e...

journal_title：Molecular biology of the cell

authors： Buttery SM,Yoshida S,Pellman D

更新日期：2007-05-01 00:00:00

abstract：:In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

journal_title：Molecular biology of the cell

authors： Kumar S,Briguglio JS,Turkewitz AP

更新日期：2014-08-15 00:00:00

abstract：:The small G proteins Cdc42, Rac1, and Rac2 regulate the rearrangements of actin and membrane necessary for Fcgamma receptor-mediated phagocytosis by macrophages. Activated, GTP-bound Cdc42, Rac1, and Rac2 bind to the p21-binding domain (PBD) of PAK1, and this interaction provided a basis for microscopic methods to loc...

journal_title：Molecular biology of the cell

authors： Hoppe AD,Swanson JA

更新日期：2004-08-01 00:00:00

abstract：:MCAK belongs to the Kinesin-13 family, whose members depolymerize microtubules rather than translocate along them. We defined the minimal functional unit of MCAK as the catalytic domain plus the class specific neck (MD-MCAK), which is consistent with previous reports. We used steady-state ATPase kinetics, microtubule ...

journal_title：Molecular biology of the cell

authors： Hertzer KM,Ems-McClung SC,Kline-Smith SL,Lipkin TG,Gilbert SP,Walczak CE

更新日期：2006-02-01 00:00:00

abstract：:Eukaryotic cells activate the unfolded-protein response (UPR) upon endoplasmic reticulum (ER) stress, where the stress is assumed to be the accumulation of unfolded proteins in the ER. Consistent with previous in vitro studies of the ER-luminal domain of the mutant UPR initiator Ire1, our study show its association wi...

journal_title：Molecular biology of the cell

authors： Promlek T,Ishiwata-Kimata Y,Shido M,Sakuramoto M,Kohno K,Kimata Y

更新日期：2011-09-01 00:00:00

abstract：:Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the structure and function of the Golgi complex. Here we further investigate the Golgin protein Coy1 and document its function in retrograde transport between early Golgi compartments. Cells that lack Coy1 displayed a reduced half-l...

journal_title：Molecular biology of the cell

authors： Anderson NS,Mukherjee I,Bentivoglio CM,Barlowe C

更新日期：2017-08-09 00:00:00

abstract：:Mutations in the mitochondrial cardiolipin (CL) transacylase, tafazzin (Taz1p), result in the X-linked cardioskeletal myopathy, Barth syndrome (BTHS). The mitochondria of BTHS patients exhibit variable respiratory defects and abnormal cristae ultrastructure. The biochemical basis for these observations is unknown. In ...

journal_title：Molecular biology of the cell

authors： Claypool SM,Boontheung P,McCaffery JM,Loo JA,Koehler CM

更新日期：2008-12-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:Cell invasion targets specific tissues in physiological placental implantation and pathological metastasis, which raises questions about how this process is controlled. We compare dermis and endometrium capacities to support trophoblast invasion, using matching sets of human primary fibroblasts in a coculture assay wi...

journal_title：Molecular biology of the cell

authors： Lopez-Mejia IC,De Toledo M,Della Seta F,Fafet P,Rebouissou C,Deleuze V,Blanchard JM,Jorgensen C,Tazi J,Vignais ML

更新日期：2013-10-01 00:00:00

abstract：:Rab GTPases are localized to various intracellular compartments and are known to play important regulatory roles in membrane trafficking. Here, we report the subcellular distribution and function of Rab14. By immunofluorescence and immunoelectron microscopy, both endogenous as well as overexpressed Rab14 were localize...

journal_title：Molecular biology of the cell

authors： Junutula JR,De Maziére AM,Peden AA,Ervin KE,Advani RJ,van Dijk SM,Klumperman J,Scheller RH

更新日期：2004-05-01 00:00:00

abstract：:The Sec61 translocon mediates the translocation of proteins across the endoplasmic reticulum membrane and the lateral integration of transmembrane segments into the lipid bilayer. The structure of the idle translocon is closed by a lumenal plug domain and a hydrophobic constriction ring. To test the function of the ap...

journal_title：Molecular biology of the cell

authors： Junne T,Kocik L,Spiess M

更新日期：2010-05-15 00:00:00

abstract：:Phosphoinositides (PIs) regulate a myriad of cellular functions including membrane fusion, as exemplified by the yeast vacuole, which uses various PIs at different stages of fusion. In light of this, the effect of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) on vacuole fusion remains unknown. PI(3,5)P2 is made by...

journal_title：Molecular biology of the cell

authors： Miner GE,Sullivan KD,Guo A,Jones BC,Hurst LR,Ellis EC,Starr ML,Fratti RA

更新日期：2019-01-15 00:00:00

abstract：:Cadherin-based cell-cell contacts are prominent sites for phosphotyrosine signaling, being enriched in tyrosine-phosphorylated proteins and tyrosine kinases and phosphatases. The functional interplay between cadherin adhesion and tyrosine kinase signaling, however, is complex and incompletely understood. In this repor...

journal_title：Molecular biology of the cell

authors： McLachlan RW,Kraemer A,Helwani FM,Kovacs EM,Yap AS

更新日期：2007-08-01 00:00:00

abstract：:The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein heavy chain and may mediate the attachment of dynein to centrosomes and o...

journal_title：Molecular biology of the cell

authors： Mische S,He Y,Ma L,Li M,Serr M,Hays TS

更新日期：2008-11-01 00:00:00

abstract：:Metaphase spindles assemble to a steady state in length by mechanisms that involve microtubule dynamics and motor proteins, but they are incompletely understood. We found that Xenopus extract spindles recapitulate the length of egg meiosis II spindles, by using mechanisms intrinsic to the spindle. To probe these mecha...

journal_title：Molecular biology of the cell

authors： Mitchison TJ,Maddox P,Gaetz J,Groen A,Shirasu M,Desai A,Salmon ED,Kapoor TM

更新日期：2005-06-01 00:00:00

abstract：:Dog thyroid epithelial cells in primary culture constitute a physiologically relevant model of positive control of DNA synthesis initiation and G0-S prereplicative phase progression by cAMP as a second messenger for thyrotropin (thyroid-stimulating hormone [TSH]). As previously shown in this system, the cAMP-dependent...

journal_title：Molecular biology of the cell

authors： Depoortere F,Pirson I,Bartek J,Dumont JE,Roger PP

更新日期：2000-03-01 00:00:00