Yeast cycloheximide-resistant crl mutants are proteasome mutants defective in protein degradation.

abstract：:The perinucleolar compartment (PNC) is a nuclear substructure present in transformed cells. The PNC is defined by high concentrations of certain RNA binding proteins and a subset of small RNAs transcribed by RNA polymerase III (pol III), including the signal recognition particle RNA and an Alu RNA as reported here. To...

journal_title：Molecular biology of the cell

authors： Wang C,Politz JC,Pederson T,Huang S

更新日期：2003-06-01 00:00:00

abstract：:Desmogleins (Dsgs) are cadherin family adhesion molecules essential for epidermal integrity. Previous studies have shown that desmogleins associate with lipid rafts, but the significance of this association was not clear. Here, we report that the desmoglein transmembrane domain (TMD) is the primary determinant of raft...

journal_title：Molecular biology of the cell

authors： Lewis JD,Caldara AL,Zimmer SE,Stahley SN,Seybold A,Strong NL,Frangakis AS,Levental I,Wahl JK 3rd,Mattheyses AL,Sasaki T,Nakabayashi K,Hata K,Matsubara Y,Ishida-Yamamoto A,Amagai M,Kubo A,Kowalczyk AP

更新日期：2019-06-01 00:00:00

abstract：:Candida albicans must undergo a switch from white to opaque to mate. Opaque cells then release mating type-specific pheromones that induce mating responses in opaque cells. Uniquely in C. albicans, the same pheromones induce mating-incompetent white cells to become cohesive, form an adhesive basal layer of cells on a ...

journal_title：Molecular biology of the cell

authors： Yi S,Sahni N,Daniels KJ,Pujol C,Srikantha T,Soll DR

更新日期：2008-03-01 00:00:00

abstract：:Bloom syndrome (BS) is a rare autosomal recessive disorder characterized by growth deficiency, immunodeficiency, genomic instability, and the early development of cancers of many types. BLM, the protein encoded by BLM, the gene mutated in BS, is localized in nuclear foci and absent from BS cells. BLM encodes a DNA hel...

journal_title：Molecular biology of the cell

authors： Neff NF,Ellis NA,Ye TZ,Noonan J,Huang K,Sanz M,Proytcheva M

更新日期：1999-03-01 00:00:00

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:Bile ducts are hepatic tubular structures that are lined by cholangiocytes, a type of liver epithelial cell. Cholangiocytes first form a single layer of cells, termed the ductal plate, surrounding the portal vein, which eventually remodels into the branching tubular network of bile ducts. The process of bile duct morp...

journal_title：Molecular biology of the cell

authors： Tanimizu N,Miyajima A,Mostov KE

更新日期：2009-05-01 00:00:00

abstract：:Damaged DNA-binding protein 2 (DDB2), a nuclear protein, participates in both nucleotide excision repair and mRNA transcription. The transcriptional regulatory function of DDB2 is significant in colon cancer, as it regulates metastasis. To characterize the mechanism by which DDB2 participates in transcription, we inve...

journal_title：Molecular biology of the cell

authors： Fantini D,Huang S,Asara JM,Bagchi S,Raychaudhuri P

更新日期：2017-01-01 00:00:00

abstract：:The process of macropinocytosis is an essential aspect of normal cell function, contributing to both growth and motile processes of cells. p21-activated kinases (PAKs) are targets for activated Rac and Cdc42 guanosine 5'-triphosphatases and have been shown to regulate the actin-myosin cytoskeleton. In fibroblasts PAK1...

journal_title：Molecular biology of the cell

authors： Dharmawardhane S,Schürmann A,Sells MA,Chernoff J,Schmid SL,Bokoch GM

更新日期：2000-10-01 00:00:00

abstract：:In Schizosaccharomyces pombe, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring (CR). A single essential formin, Cdc12, localizes to the cell middle upon mitotic onset and nucleates the F-actin of the CR. Cdc12 medial recruitment is mediated in part by its direct binding to the...

journal_title：Molecular biology of the cell

authors： Willet AH,Bohnert KA,Gould KL

更新日期：2018-03-15 00:00:00

abstract：:Endocytic adaptor proteins facilitate cargo recruitment and clathrin-coated pit nucleation. The prototypical clathrin adaptor AP2 mediates cargo recruitment, maturation, and scission of the pit by binding cargo, clathrin, and accessory proteins, including the Eps-homology (EH) domain proteins Eps15 and intersectin. Ho...

journal_title：Molecular biology of the cell

authors： Teckchandani A,Mulkearns EE,Randolph TW,Toida N,Cooper JA

更新日期：2012-08-01 00:00:00

abstract：:The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

journal_title：Molecular biology of the cell

authors： Melvin A,Mudie S,Rocha S

更新日期：2011-11-01 00:00:00

abstract：:The mitotic exit network (MEN) is an essential GTPase signaling pathway that triggers exit from mitosis in budding yeast. We show here that during meiosis, the MEN is dispensable for exit from meiosis I but contributes to the timely exit from meiosis II. Consistent with a role for the MEN during meiosis II, we find th...

journal_title：Molecular biology of the cell

authors： Attner MA,Amon A

更新日期：2012-08-01 00:00:00

abstract：:Chemoattractants induce neutrophil polarization through localized polymerization of F-actin at the leading edge. The suppression of rear and lateral protrusions is required for efficient chemotaxis and involves the temporal and spatial segregation of signaling molecules. We have previously shown that the intracellular...

journal_title：Molecular biology of the cell

authors： Nuzzi PA,Senetar MA,Huttenlocher A

更新日期：2007-03-01 00:00:00

abstract：:As yeast cultures enter stationary phase in rich, glucose-based medium, differentiation of two major subpopulations of cells, termed quiescent and nonquiescent, is observed. Differences in mRNA abundance between exponentially growing and stationary-phase cultures and quiescent and nonquiescent cells are known, but lit...

journal_title：Molecular biology of the cell

authors： Davidson GS,Joe RM,Roy S,Meirelles O,Allen CP,Wilson MR,Tapia PH,Manzanilla EE,Dodson AE,Chakraborty S,Carter M,Young S,Edwards B,Sklar L,Werner-Washburne M

更新日期：2011-04-01 00:00:00

abstract：:The role of platelet endothelial cell adhesion molecule-1 (PECAM-1) in endothelial cell-cell interactions and its contribution to cadherin-mediated cell adhesion are poorly understood. Such studies have been difficult because all known endothelial cells express PECAM-1. We have used Madin-Darby canine kidney (MDCK) ce...

journal_title：Molecular biology of the cell

authors： Sheibani N,Sorenson CM,Frazier WA

更新日期：2000-08-01 00:00:00

abstract：:During interactions with its mammalian host, the pathogenic yeast Candida albicans is exposed to a range of stresses such as superoxide radicals and cationic fluxes. Unexpectedly, a nonbiased screen of transcription factor deletion mutants revealed that the phosphate-responsive transcription factor Pho4 is vital for t...

journal_title：Molecular biology of the cell

authors： Ikeh MA,Kastora SL,Day AM,Herrero-de-Dios CM,Tarrant E,Waldron KJ,Banks AP,Bain JM,Lydall D,Veal EA,MacCallum DM,Erwig LP,Brown AJ,Quinn J

更新日期：2016-09-01 00:00:00

abstract：:Polalpha is the principal DNA polymerase for initiation of DNA replication and also functions in postinitiation DNA synthesis. In this study, we investigated the cell cycle responses induced by mutations in polalpha+. Germinating spores carrying either a deletion of polalpha+ (polalphaDelta) or a structurally intact b...

journal_title：Molecular biology of the cell

authors： Bhaumik D,Wang TS

更新日期：1998-08-01 00:00:00

abstract：:Adaptins are subunits of adaptor protein (AP) complexes involved in the formation of intracellular transport vesicles and in the selection of cargo for incorporation into the vesicles. In this article, we report the results of a survey for adaptins from sequenced genomes including those of man, mouse, the fruit fly Dr...

journal_title：Molecular biology of the cell

authors： Boehm M,Bonifacino JS

更新日期：2001-10-01 00:00:00

abstract：:Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Perrin BJ,Bennin DA,Huttenlocher A

更新日期：2010-01-01 00:00:00

abstract：:The intermembrane space of mitochondria accommodates the essential mitochondrial intermembrane space assembly (MIA) machinery that catalyzes oxidative folding of proteins. The disulfide bond formation pathway is based on a relay of reactions involving disulfide transfer from the sulfhydryl oxidase Erv1 to Mia40 and fr...

journal_title：Molecular biology of the cell

authors： Böttinger L,Gornicka A,Czerwik T,Bragoszewski P,Loniewska-Lwowska A,Schulze-Specking A,Truscott KN,Guiard B,Milenkovic D,Chacinska A

更新日期：2012-10-01 00:00:00

abstract：:The subcellular distribution of kinases and other signaling proteins is regulated in response to cellular cues; however, the extent of this regulation has not been investigated for any gene set in any organism. Here, we present a systematic analysis of protein kinases in the budding yeast, screening for differential l...

journal_title：Molecular biology of the cell

authors： Bharucha N,Ma J,Dobry CJ,Lawson SK,Yang Z,Kumar A

更新日期：2008-07-01 00:00:00

abstract：:Insulin and hypertonicity each increase the content of GLUT4 glucose transporters at the surface of muscle cells. Insulin enhances GLUT4 exocytosis without diminishing its endocytosis. The insulin but not the hypertonicity response is reduced by tetanus neurotoxin, which cleaves vesicle-associated membrane protein (VA...

journal_title：Molecular biology of the cell

authors： Randhawa VK,Thong FS,Lim DY,Li D,Garg RR,Rudge R,Galli T,Rudich A,Klip A

更新日期：2004-12-01 00:00:00

abstract：:DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we express...

journal_title：Molecular biology of the cell

authors： Lee HO,Zacharek SJ,Xiong Y,Duronio RJ

更新日期：2010-11-01 00:00:00

abstract：:A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

journal_title：Molecular biology of the cell

authors： Korobova F,Svitkina T

更新日期：2008-04-01 00:00:00

abstract：:ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response (UPR), which up-regulates genes that enable cells to cope with misfolded, ER-retained proteins. In this study, we compare th...

journal_title：Molecular biology of the cell

authors： Metzger MB,Michaelis S

更新日期：2009-02-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to bett...

journal_title：Molecular biology of the cell

authors： Jiang P,Nishimura T,Sakamaki Y,Itakura E,Hatta T,Natsume T,Mizushima N

更新日期：2014-04-01 00:00:00

abstract：:We have shown previously that the Swi5 transcription factor regulates the expression of the SIC1 Cdk inhibitor in late mitosis. This suggests that Swi5 might control other genes with roles in ending mitosis. We identified a gene with a Swi5-binding site in the promoter that encoded a protein with high homology to Pcl2...

journal_title：Molecular biology of the cell

authors： Aerne BL,Johnson AL,Toyn JH,Johnston LH

更新日期：1998-04-01 00:00:00

abstract：:EB1 is a conserved microtubule plus end tracking protein considered to play crucial roles in microtubule organization and the interaction of microtubules with the cell cortex. Despite intense studies carried out in yeast and cultured cells, the role of EB1 in multicellular systems remains to be elucidated. Here, we de...

journal_title：Molecular biology of the cell

authors： Elliott SL,Cullen CF,Wrobel N,Kernan MJ,Ohkura H

更新日期：2005-02-01 00:00:00

abstract：:Integrin-mediated adhesion induces several signaling pathways leading to regulation of gene transcription, control of cell cycle entry and survival from apoptosis. Here we investigate the involvement of the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) pathway in integrin-mediated signal...

journal_title：Molecular biology of the cell

authors： Brizzi MF,Defilippi P,Rosso A,Venturino M,Garbarino G,Miyajima A,Silengo L,Tarone G,Pegoraro L

更新日期：1999-10-01 00:00:00