The same receptor, G protein, and mitogen-activated protein kinase pathway activate different downstream regulators in the alternative white and opaque pheromone responses of Candida albicans.

abstract：:Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disr...

journal_title：Molecular biology of the cell

authors： Zeng C,Anjard C,Riemann K,Konzok A,Nellen W

更新日期：2000-05-01 00:00:00

abstract：:We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

journal_title：Molecular biology of the cell

authors： McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

更新日期：2011-11-01 00:00:00

abstract：:Epidermolysis bullosa simplex associated with late-onset muscular dystrophy (EBS-MD) is an autosomal recessive disorder resulting from mutations in the plectin gene. The majority of these mutations occur within the large exon 31 encoding the central rod domain and leave the production of a low-level rodless plectin sp...

journal_title：Molecular biology of the cell

authors： Ketema M,Secades P,Kreft M,Nahidiazar L,Janssen H,Jalink K,de Pereda JM,Sonnenberg A

更新日期：2015-07-01 00:00:00

abstract：:The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

journal_title：Molecular biology of the cell

authors： Melvin A,Mudie S,Rocha S

更新日期：2011-11-01 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:The Notch signaling pathway plays essential roles in both animal development and human disease. Regulation of Notch receptor levels in membrane compartments has been shown to affect signaling in a variety of contexts. Here we used steady-state and pulse-labeling techniques to follow Notch receptors in sensory organ pr...

journal_title：Molecular biology of the cell

authors： Johnson SA,Zitserman D,Roegiers F

更新日期：2016-09-15 00:00:00

abstract：:The preprotein translocase of the outer mitochondrial membrane (TOM) consists of a central β-barrel channel, Tom40, and six proteins with α-helical transmembrane segments. The precursor of Tom40 is imported from the cytosol by a pre-existing TOM complex and inserted into the outer membrane by the sorting and assembly ...

journal_title：Molecular biology of the cell

authors： Becker T,Guiard B,Thornton N,Zufall N,Stroud DA,Wiedemann N,Pfanner N

更新日期：2010-09-15 00:00:00

abstract：:The Drosophila tumor suppressor protein lethal (2) giant larvae [l(2)gl] is involved in the establishment of epithelial cell polarity during development. Recently, a yeast homolog of the protein has been shown to interact with components of the post-Golgi exocytic machinery and to regulate a late step in protein secre...

journal_title：Molecular biology of the cell

authors： Müsch A,Cohen D,Yeaman C,Nelson WJ,Rodriguez-Boulan E,Brennwald PJ

更新日期：2002-01-01 00:00:00

abstract：:In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additional...

journal_title：Molecular biology of the cell

authors： Jovanovic OA,Brown FD,Donaldson JG

更新日期：2006-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the class C vacuole protein sorting (Vps) proteins, together with Vam2p/Vps41p and Vam6p/Vps39p, form a complex that interacts with soluble N-ethylmaleimide-sensitive factor attachment protein receptor and Rab proteins to "tether" vacuolar membranes before fusion. To determine a role for t...

journal_title：Molecular biology of the cell

authors： Richardson SC,Winistorfer SC,Poupon V,Luzio JP,Piper RC

更新日期：2004-03-01 00:00:00

abstract：:Most membrane proteins are synthesized on and inserted into the membrane of the endoplasmic reticulum (ER), in eukaryote. The widely conserved ER membrane protein complex (EMC) facilitates the biogenesis of a wide range of membrane proteins. In this study, we investigated the EMC function using Drosophila photorecepto...

journal_title：Molecular biology of the cell

authors： Hiramatsu N,Tago T,Satoh T,Satoh AK

更新日期：2019-11-01 00:00:00

abstract：:We report here that Trypanosoma cruzi, the etiologic agent of Chagas disease, possesses two unique paralogues of the mitochondrial calcium uniporter complex TcMCU subunit that we named TcMCUc and TcMCUd. The predicted structure of the proteins indicates that, as predicted for the TcMCU and TcMCUb paralogues, they are ...

journal_title：Molecular biology of the cell

authors： Chiurillo MA,Lander N,Bertolini MS,Vercesi AE,Docampo R

更新日期：2019-07-01 00:00:00

abstract：:The scaffold protein XB130 regulates cell growth, survival, and migration. Yeast two-hybrid screening suggests that XB130 interacts with another scaffold protein, Tks5. We hypothesized that XB130 and Tks5 form a macromolecular complex to mediate signal transduction cascades for the regulation of cell growth and surviv...

journal_title：Molecular biology of the cell

authors： Moodley S,Hui Bai X,Kapus A,Yang B,Liu M

更新日期：2015-12-01 00:00:00

abstract：:Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

journal_title：Molecular biology of the cell

authors： Zhou D,Ursitti JA,Bloch RJ

更新日期：1998-01-01 00:00:00

abstract：:A cornerstone of the antiviral interferon response is phosphorylation of eukaryotic initiation factor (eIF)2alpha. This limits the availability of eIF2.GTP.Met-tRNA(i)(Met) ternary complexes, reduces formation of 43S preinitiation complexes, and blocks viral (and most cellular) mRNA translation. However, many viruses ...

journal_title：Molecular biology of the cell

authors： Robert F,Kapp LD,Khan SN,Acker MG,Kolitz S,Kazemi S,Kaufman RJ,Merrick WC,Koromilas AE,Lorsch JR,Pelletier J

更新日期：2006-11-01 00:00:00

abstract：:We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication. Using immunoblotting, immunofluorescence, and high-speed automated cell-imaging analyses with antibodies specific against MCM2 and C...

journal_title：Molecular biology of the cell

authors： Tsuji T,Ficarro SB,Jiang W

更新日期：2006-10-01 00:00:00

abstract：:Many integrin receptors localize to focal contact sites upon binding their ligand. However, unoccupied integrin receptors do not localize to focal contact sites. Because the integrin beta 1 cytoplasmic domain appears to have a focal contact localization signal, there must be a mechanism by which this domain is kept in...

journal_title：Molecular biology of the cell

authors： Briesewitz R,Kern A,Marcantonio EE

更新日期：1993-06-01 00:00:00

abstract：:Androgen receptor (AR) belongs to the nuclear receptor superfamily and mediates the biological actions of male sex steroids. In this work, we have characterized a novel 130-kDa Ser/Thr protein kinase ANPK that interacts with the zinc finger region of AR in vivo and in vitro. The catalytic kinase domain of ANPK shares ...

journal_title：Molecular biology of the cell

authors： Moilanen AM,Karvonen U,Poukka H,Jänne OA,Palvimo JJ

更新日期：1998-09-01 00:00:00

abstract：:Maturation-promoting factor, consisting of cdc2 protein kinase and a regulatory B-type cyclin, is a universal regulator of meiosis and mitosis in eukaryotes. In Xenopus, there are two subtypes of B-type cyclins, designated B1 and B2, both of which are phosphorylated. In this study, we have investigated the biological ...

journal_title：Molecular biology of the cell

authors： Li J,Meyer AN,Donoghue DJ

更新日期：1995-09-01 00:00:00

abstract：:Lipoprotein(a) (Lp(a)) is associated with cardiovascular disease risk. This may be attributable to the ability of Lp(a) to elicit endothelial dysfunction. We previously reported that apolipoprotein(a) (apo(a); the distinguishing kringle-containing component of Lp(a)) elicits cytoskeletal rearrangements in vascular end...

journal_title：Molecular biology of the cell

authors： Cho T,Romagnuolo R,Scipione C,Boffa MB,Koschinsky ML

更新日期：2013-02-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00

abstract：:Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR) resides in the nucleo...

journal_title：Molecular biology of the cell

authors： Jády BE,Richard P,Bertrand E,Kiss T

更新日期：2006-02-01 00:00:00

abstract：:The Wnt signaling pathway plays a major role in development, and upon deregulation it is implicated in neoplasia. The hallmark of the canonical Wnt signal is the protection of beta-catenin from ubiquitination and proteasomal degradation induced by glycogen synthase kinase (GSK)-3beta inhibition. The stabilized beta-ca...

journal_title：Molecular biology of the cell

authors： Bachar-Dahan L,Goltzmann J,Yaniv A,Gazit A

更新日期：2006-06-01 00:00:00

abstract：:The BNIP-2 and Cdc42GAP homology (BCH) domain is a novel regulator for Rho GTPases, but its impact on p50-Rho GTPase-activating protein (p50RhoGAP or Cdc42GAP) in cells remains elusive. Here we show that deletion of the BCH domain from p50RhoGAP enhanced its GAP activity and caused drastic cell rounding. Introducing c...

journal_title：Molecular biology of the cell

authors： Zhou YT,Chew LL,Lin SC,Low BC

更新日期：2010-09-15 00:00:00

abstract：:Scientific societies aiming to foster inclusion of scientists from underrepresented (UR) backgrounds among their membership often delegate primary responsibility for this goal to a diversity-focused committee. The National Science Foundation has funded the creation of the Alliance to Catalyze Change for Equity in STEM...

journal_title：Molecular biology of the cell

authors： Segarra VA,Primus C,Unguez GA,Edwards A,Etson C,Flores SC,Fry C,Guillory AN,Ingram SL,Lawson M,McGee R,Paxson S,Phelan L,Suggs K,Vega LR,Vuong E,Havran JC,Leon A,Burton MD,Lujan JL,Ramirez-Alvarado M

更新日期：2020-11-01 00:00:00

abstract：:Endomitosis is a special type of mitosis in which only cytokinesis-the final step of the cell division cycle-is defective, resulting in polyploid cells. Although endomitosis is biologically important, its regulatory aspects remain elusive. Psychosine, a lysogalactosylceramide, prevents proper cytokinesis when suppleme...

journal_title：Molecular biology of the cell

authors： Watanabe H,Okahara K,Naito-Matsui Y,Abe M,Go S,Inokuchi J,Okazaki T,Kobayashi T,Kozutsumi Y,Oka S,Takematsu H

更新日期：2016-07-01 00:00:00

abstract：:Cilia and flagella are structurally and functionally conserved organelles present in basal as well as higher eukaryotes. The assembly of cilia requires a microtubule based scaffold called a basal body. The ninefold symmetry characteristic of basal bodies and the structurally similar centriole is organized around a hub...

journal_title：Molecular biology of the cell

authors： Culver BP,Meehl JB,Giddings TH Jr,Winey M

更新日期：2009-03-01 00:00:00

abstract：:Protein kinase D2 (PKD2) belongs to the PKD family of serine/threonine kinases that is activated by phorbol esters and G protein-coupled receptors (GPCRs). Its C-terminal regulatory domain comprises two cysteine-rich domains (C1a/C1b) followed by a pleckstrin homology (PH) domain. Here, we examined the role of the reg...

journal_title：Molecular biology of the cell

authors： Auer A,von Blume J,Sturany S,von Wichert G,Van Lint J,Vandenheede J,Adler G,Seufferlein T

更新日期：2005-09-01 00:00:00

abstract：:An increase in the level of active, GTP-bound Ras is not necessary for transformation of chicken embryo fibroblasts (CEF) by v-Src. This suggests that other Ras-independent pathways contribute to transformation by v-Src. To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammali...

journal_title：Molecular biology of the cell

authors： Penuel E,Martin GS

更新日期：1999-06-01 00:00:00

abstract：:CD98, an early marker of T-cell activation, is an important regulator of integrin-mediated adhesion events. Previous studies suggest that CD98 is coupled to both cellular activation and transformation and is involved in the pathogenesis of viral infection, inflammatory disease, and cancer. Understanding of the molecul...

journal_title：Molecular biology of the cell

authors： Rintoul RC,Buttery RC,Mackinnon AC,Wong WS,Mosher D,Haslett C,Sethi T

更新日期：2002-08-01 00:00:00