Abstract:
:The Notch signaling pathway plays essential roles in both animal development and human disease. Regulation of Notch receptor levels in membrane compartments has been shown to affect signaling in a variety of contexts. Here we used steady-state and pulse-labeling techniques to follow Notch receptors in sensory organ precursor cells in Drosophila. We find that the endosomal adaptor protein Numb regulates levels of Notch receptor trafficking to Rab7-labeled late endosomes but not early endosomes. Using an assay we developed that labels different pools of Notch receptors as they move through the endocytic system, we show that Numb specifically suppresses a recycled Notch receptor subpopulation and that excess Notch signaling in numb mutants requires the recycling endosome GTPase Rab11 activity. Our data therefore suggest that Numb controls the balance between Notch receptor recycling and receptor targeting to late endosomes to regulate signaling output after asymmetric cell division in Drosophila neural progenitors.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Johnson SA,Zitserman D,Roegiers Fdoi
10.1091/mbc.E15-11-0751subject
Has Abstractpub_date
2016-09-15 00:00:00pages
2857-66issue
18eissn
1059-1524issn
1939-4586pii
mbc.E15-11-0751journal_volume
27pub_type
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