Localization and possible functions of Drosophila septins.


:The septins are a family of homologous proteins that were originally identified in Saccharomyces cerevisiae, where they are associated with the "neck filaments" and are involved in cytokinesis and other aspects of the organization of the cell surface. We report here the identification of Sep1, a Drosophila melanogaster septin, based on its homology to the yeast septins. The predicted Sep1 amino acid sequence is 35-42% identical to the known S. cerevisiae septins; 52% identical to Pnut, a second D. melanogaster septin; and 53-73% identical to the known mammalian septins. Sep1-specific antibodies have been used to characterize its expression and localization. The protein is concentrated at the leading edge of the cleavage furrows of dividing cells and cellularizing embryos, suggesting a role in furrow formation. Other aspects of Sep1 localization suggest roles not directly related to cytokinesis. For example, Sep1 exhibits orderly, cell-cycle-coordinated rearrangements within the cortex of syncytial blastoderm embryos and in the cells of post-gastrulation embryos; Sep1 is also concentrated at the leading edge of the epithelium during dorsal closure in the embryo, in the neurons of the embryonic nervous system, and at the baso-lateral surfaces of ovarian follicle cells. The distribution of Sep1 typically overlaps, but is distinct from, that of actin. Both immunolocalization and biochemical experiments show that Sep1 is intimately associated with Pnut, suggesting that the Drosophila septins, like those in yeast, function as part of a complex.


Mol Biol Cell


Fares H,Peifer M,Pringle JR




Has Abstract


1995-12-01 00:00:00












  • Matrix-independent survival of human keratinocytes through an EGF receptor/MAPK-kinase-dependent pathway.

    abstract::Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jost M,Huggett TM,Kari C,Rodeck U

    更新日期:2001-05-01 00:00:00

  • Overexpression of a novel rho family GTPase, RacC, induces unusual actin-based structures and positively affects phagocytosis in Dictyostelium discoideum.

    abstract::Rho family proteins have been implicated in regulating various cellular processes, including actin cytoskeleton organization, endocytosis, cell cycle, and gene expression. In this study, we analyzed the function of a novel Dictyostelium discoideum Rho family protein (RacC). A cell line was generated that conditionally...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Seastone DJ,Lee E,Bush J,Knecht D,Cardelli J

    更新日期:1998-10-01 00:00:00

  • SNAP-23 regulates phagosome formation and maturation in macrophages.

    abstract::Synaptosomal associated protein of 23 kDa (SNAP-23), a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), has been implicated in phagocytosis by macrophages. For elucidation of its precise role in this process, a macrophage line overexpressing monomeric Venus-tagge...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Sakurai C,Hashimoto H,Nakanishi H,Arai S,Wada Y,Sun-Wada GH,Wada I,Hatsuzawa K

    更新日期:2012-12-01 00:00:00

  • Yeast exocytic v-SNAREs confer endocytosis.

    abstract::In yeast, homologues of the synaptobrevin/VAMP family of v-SNAREs (Snc1 and Snc2) confer the docking and fusion of secretory vesicles at the cell surface. As no v-SNARE has been shown to confer endocytosis, we examined whether yeast lacking the SNC genes, or possessing a temperature-sensitive allele of SNC1 (SNC1(ala4...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Gurunathan S,Chapman-Shimshoni D,Trajkovic S,Gerst JE

    更新日期:2000-10-01 00:00:00

  • In vitro assembly of coiled bodies in Xenopus egg extract.

    abstract::When demembranated sperm nuclei are placed in a Xenopus egg extract, they become surrounded by a nuclear envelope and then swell to form morphologically typical pronuclei. Granules ranging from < 1.0 to approximately 3.0 microns in diameter appear within such nuclei. Bell et al. identified four nucleolar proteins in t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bauer DW,Murphy C,Wu Z,Wu CH,Gall JG

    更新日期:1994-06-01 00:00:00

  • Mitogen-activated protein kinase phosphatase 2 regulates histone H3 phosphorylation via interaction with vaccinia-related kinase 1.

    abstract::Mitogen-activated protein kinase phosphatase 2 (MKP2) is a member of the dual-specificity MKPs that regulate MAP kinase signaling. However, MKP2 functions are still largely unknown. In this study, we showed that MKP2 could regulate histone H3 phosphorylation under oxidative stress conditions. We found that MKP2 inhibi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jeong MW,Kang TH,Kim W,Choi YH,Kim KT

    更新日期:2013-02-01 00:00:00

  • Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells.

    abstract::With the use of the patch clamp technique, the role of cytoskeleton in the regulation of ion channels in plasma membrane of leukemic K562 cells was examined. Single-channel measurements have indicated that disruption of actin filaments with cytochalasin D (CD) resulted in a considerable increase of the activity of non...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Negulyaev YA,Vedernikova EA,Maximov AV

    更新日期:1996-12-01 00:00:00

  • SERCaMP: a carboxy-terminal protein modification that enables monitoring of ER calcium homeostasis.

    abstract::Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

    更新日期:2014-09-15 00:00:00

  • Time-varying mobility and turnover of actomyosin ring components during cytokinesis in Schizosaccharomyces pombe.

    abstract::Cytokinesis in many eukaryotes is dependent on a contractile actomyosin ring (AMR), composed of F-actin, myosin II, and other actin and myosin II regulators. Through fluorescence recovery after photobleaching experiments, many components of the AMR have been shown to be mobile and to undergo constant exchange with the...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kamnev A,Palani S,Zambon P,Cheffings T,Burroughs N,Balasubramanian MK

    更新日期:2021-02-01 00:00:00

  • Myosin vb is associated with plasma membrane recycling systems.

    abstract::Myosin Va is associated with discrete vesicle populations in a number of cell types, but little is known of the function of myosin Vb. Yeast two-hybrid screening of a rabbit parietal cell cDNA library with dominant active Rab11a (Rab11aS20V) identified myosin Vb as an interacting protein for Rab11a, a marker for plasm...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lapierre LA,Kumar R,Hales CM,Navarre J,Bhartur SG,Burnette JO,Provance DW Jr,Mercer JA,Bähler M,Goldenring JR

    更新日期:2001-06-01 00:00:00

  • Phosphorylation of the effector complex HOPS by the vacuolar kinase Yck3p confers Rab nucleotide specificity for vacuole docking and fusion.

    abstract::The homotypic fusion of yeast vacuoles requires the Rab-family GTPase Ypt7p and its effector complex, homotypic fusion and vacuole protein sorting complex (HOPS). Although the vacuolar kinase Yck3p is required for the sensitivity of vacuole fusion to proteins that regulate the Rab GTPase cycle-Gdi1p (GDP-dissociation ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Zick M,Wickner W

    更新日期:2012-09-01 00:00:00

  • Evidence for the presence of 5S rRNA in mammalian mitochondria.

    abstract::Mammalian mitochondrial ribosomes contain two prokaryotic-like rRNAs, 12S and 16S, both encoded by mitochondrial DNA. As opposed to cytosolic ribosomes, however, these ribosomes are not thought to contain 5S rRNA. For this reason, it has been unclear whether 5S rRNA, which can be detected in mitochondrial preparations...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Magalhães PJ,Andreu AL,Schon EA

    更新日期:1998-09-01 00:00:00

  • Saccharomyces cerevisiae PTS1 receptor Pex5p interacts with the SH3 domain of the peroxisomal membrane protein Pex13p in an unconventional, non-PXXP-related manner.

    abstract::A number of peroxisome-associated proteins have been described that are involved in the import of proteins into peroxisomes, among which is the receptor for peroxisomal targeting signal 1 (PTS1) proteins Pex5p, the integral membrane protein Pex13p, which contains an Src homology 3 (SH3) domain, and the peripheral memb...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bottger G,Barnett P,Klein AT,Kragt A,Tabak HF,Distel B

    更新日期:2000-11-01 00:00:00

  • Phosphatidic acid induces ligand-independent epidermal growth factor receptor endocytic traffic through PDE4 activation.

    abstract::Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

    更新日期:2010-08-15 00:00:00

  • Developing a set of guidelines for your research field: a practical approach.

    abstract::Since 2008, the autophagy community has periodically published a set of guidelines, currently titled "Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy." The newest version of the guidelines was published in 2016. There are many reasons for establishing a set of guidelines in a given researc...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Klionsky DJ

    更新日期:2016-03-01 00:00:00

  • Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects.

    abstract::Loss-of-function mutations in the nonconventional myosin Vb (Myo5b) result in microvillus inclusion disease (MVID) and massive secretory diarrhea that often begins at birth. Myo5b mutations disrupt the apical recycling endosome (ARE) and membrane traffic, resulting in reduced surface expression of apical membrane prot...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Forteza R,Ahsan MK,Cartón-García F,Arango D,Ameen NA,Salas PJ

    更新日期:2019-12-15 00:00:00

  • Targeting of a tail-anchored protein to endoplasmic reticulum and mitochondrial outer membrane by independent but competing pathways.

    abstract::Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins posttranslationally select their target, but C-terminal charged residues play an important role. To investigate how discrimination b...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Borgese N,Gazzoni I,Barberi M,Colombo S,Pedrazzini E

    更新日期:2001-08-01 00:00:00

  • Multilayered regulation of TORC1-body formation in budding yeast.

    abstract::The target of rapamycin kinase complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. In Saccharomyces cerevisiae, TORC1 activity is known to be controlled by the conserved GTPases, Gtr1/2, and movement into and out of an inactive agglomerate/body. However, it is unclear whether/how these regulatory ste...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Sullivan A,Wallace RL,Wellington R,Luo X,Capaldi AP

    更新日期:2019-02-01 00:00:00

  • Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.

    abstract::Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p lo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Pollard LW,Onishi M,Pringle JR,Lord M

    更新日期:2012-07-01 00:00:00

  • Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.

    abstract::A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Korobova F,Svitkina T

    更新日期:2008-04-01 00:00:00

  • Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells.

    abstract::Phospholipase Ds (PLDs) are regulated enzymes that generate phosphatidic acid (PA), a putative second messenger implicated in the regulation of vesicular trafficking and cytoskeletal reorganization. Mast cells, when stimulated with antigen, show a dramatic alteration in their cytoskeleton and also release their secret...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: O'Luanaigh N,Pardo R,Fensome A,Allen-Baume V,Jones D,Holt MR,Cockcroft S

    更新日期:2002-10-01 00:00:00

  • Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy.

    abstract::In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kabeya Y,Kamada Y,Baba M,Takikawa H,Sasaki M,Ohsumi Y

    更新日期:2005-05-01 00:00:00

  • Tumor necrosis factor-receptor-associated factor-4 is a positive regulator of transforming growth factor-beta signaling that affects neural crest formation.

    abstract::The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kalkan T,Iwasaki Y,Park CY,Thomsen GH

    更新日期:2009-07-01 00:00:00

  • Transforming growth factor beta 1 (TGF beta 1) reduces cellular levels of p34cdc2, and this effect is abrogated by adenovirus independently of the E1A-associated pRB binding activity.

    abstract::We have used E1A probes to study the roles of the p34cdc2 kinase and the retinoblastoma tumor susceptibility gene product (pRB) in transforming growth factor beta 1 (TGF beta 1)-mediated growth suppression in mink lung epithelial (Mv1Lu) cells. In agreement with previous reports, we see a decline in p34cdc2 kinase act...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Abraham SE,Carter MC,Moran E

    更新日期:1992-06-01 00:00:00

  • Leishmania donovani restricts mitochondrial dynamics to enhance miRNP stability and target RNA repression in host macrophages.

    abstract::MicroRNAs (miRNAs), the tiny regulatory RNAs, form complexes with Argonaute (Ago) proteins and inhibit gene expression in metazoan cells. While studying parasite-invaded macrophages, we identify a unique mode of gene regulation in which the parasite Leishmania donovani (Ld) causes mitochondrial depolarization, reduces...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chakrabarty Y,Bhattacharyya SN

    更新日期:2017-07-15 00:00:00

  • HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis.

    abstract::Molecular chaperones play key roles during growth, development, and stress survival. The ability to induce chaperone expression enables cells to cope with the accumulation of nonnative proteins under stress and complete developmental processes with an increased requirement for chaperone assistance. Here we generate an...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Rogon C,Ulbricht A,Hesse M,Alberti S,Vijayaraj P,Best D,Adams IR,Magin TM,Fleischmann BK,Höhfeld J

    更新日期:2014-08-01 00:00:00

  • Natural chromatin is heterogeneous and self-associates in vitro.

    abstract::The 30-nm fiber is commonly formed by oligonucleosome arrays in vitro but rarely found inside cells. To determine how chromatin higher-order structure is controlled, we used electron cryotomography (cryo-ET) to study the undigested natural chromatin released from two single-celled organisms in which 30-nm fibers have ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Cai S,Song Y,Chen C,Shi J,Gan L

    更新日期:2018-07-01 00:00:00

  • In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties.

    abstract::Outer dynein arms (ODAs) are multiprotein complexes that drive flagellar beating. Based on genetic and biochemical analyses, ODAs preassemble in the cell body and then move into the flagellum by intraflagellar transport (IFT). To study ODA transport in vivo, we expressed the essential intermediate chain 2 tagged with ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Dai J,Barbieri F,Mitchell DR,Lechtreck KF

    更新日期:2018-10-15 00:00:00

  • Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren.

    abstract::In vitro studies suggest that the Barren protein may function as an activator of DNA topoisomerase II and/or as a component of the Xenopus condensin complex. To better understand the role of Barren in vivo, we generated conditional alleles of the structural gene for Barren (BRN1) in Saccharomyces cerevisiae. We show t...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lavoie BD,Tuffo KM,Oh S,Koshland D,Holm C

    更新日期:2000-04-01 00:00:00

  • Developmental expression of spectrins in rat skeletal muscle.

    abstract::Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Zhou D,Ursitti JA,Bloch RJ

    更新日期:1998-01-01 00:00:00