Localization and possible functions of Drosophila septins.


:The septins are a family of homologous proteins that were originally identified in Saccharomyces cerevisiae, where they are associated with the "neck filaments" and are involved in cytokinesis and other aspects of the organization of the cell surface. We report here the identification of Sep1, a Drosophila melanogaster septin, based on its homology to the yeast septins. The predicted Sep1 amino acid sequence is 35-42% identical to the known S. cerevisiae septins; 52% identical to Pnut, a second D. melanogaster septin; and 53-73% identical to the known mammalian septins. Sep1-specific antibodies have been used to characterize its expression and localization. The protein is concentrated at the leading edge of the cleavage furrows of dividing cells and cellularizing embryos, suggesting a role in furrow formation. Other aspects of Sep1 localization suggest roles not directly related to cytokinesis. For example, Sep1 exhibits orderly, cell-cycle-coordinated rearrangements within the cortex of syncytial blastoderm embryos and in the cells of post-gastrulation embryos; Sep1 is also concentrated at the leading edge of the epithelium during dorsal closure in the embryo, in the neurons of the embryonic nervous system, and at the baso-lateral surfaces of ovarian follicle cells. The distribution of Sep1 typically overlaps, but is distinct from, that of actin. Both immunolocalization and biochemical experiments show that Sep1 is intimately associated with Pnut, suggesting that the Drosophila septins, like those in yeast, function as part of a complex.


Mol Biol Cell


Fares H,Peifer M,Pringle JR




Has Abstract


1995-12-01 00:00:00












  • Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

    abstract::Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1(+), encodes a protein with three LIM domains that is similar to paxil...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Pinar M,Coll PM,Rincón SA,Pérez P

    更新日期:2008-04-01 00:00:00

  • Beta 8 integrins mediate interactions of chick sensory neurons with laminin-1, collagen IV, and fibronectin.

    abstract::Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Venstrom K,Reichardt L

    更新日期:1995-04-01 00:00:00

  • Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis.

    abstract::The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1. Hof1 colocalizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to the actomyosin ring (AMR). Neither the function of Hof1 a...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Meitinger F,Palani S,Hub B,Pereira G

    更新日期:2013-05-01 00:00:00

  • Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.

    abstract::The fission yeast Mcs6-Mcs2-Pmh1 complex, homologous to metazoan Cdk7-cyclin H-Mat1, has dual functions in cell division and transcription: as a partially redundant cyclin-dependent kinase (CDK)-activating kinase (CAK) that phosphorylates the major cell cycle CDK, Cdc2, on Thr-167; and as the RNA polymerase (Pol) II c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lee KM,Miklos I,Du H,Watt S,Szilagyi Z,Saiz JE,Madabhushi R,Penkett CJ,Sipiczki M,Bähler J,Fisher RP

    更新日期:2005-06-01 00:00:00

  • Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S. cerevisiae.

    abstract::Inactivation of YME1 in yeast causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment. T...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Weber ER,Hanekamp T,Thorsness PE

    更新日期:1996-02-01 00:00:00

  • Cytoplasmic tails of beta 1, beta 2, and beta 7 integrins differentially regulate LFA-1 function in K562 cells.

    abstract::The beta 2 integrin lymphocyte function-associated antigen 1 (LFA-1) mediates activation-dependent adhesion of lymphocytes. To investigate whether lymphocyte-specific elements are essential for LFA-1 function, we expressed LFA-1 in the erythroleukemic cell line K562, which expresses only the integrin very late antigen...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Lub M,van Vliet SJ,Oomen SP,Pieters RA,Robinson M,Figdor CG,van Kooyk Y

    更新日期:1997-04-01 00:00:00

  • Mutations in Saccharomyces cerevisiae gene SIR2 can have differential effects on in vivo silencing phenotypes and in vitro histone deacetylation activity.

    abstract::The yeast SIR2 gene and many of its homologs have been identified as NAD(+)-dependent histone deacetylases. To get a broader view of the relationship between the histone deacetylase activity of Sir2p and its in vivo functions we have mutated eight highly conserved residues in the core domain of SIR2. These mutations h...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Armstrong CM,Kaeberlein M,Imai SI,Guarente L

    更新日期:2002-04-01 00:00:00

  • Condensin suppresses recombination and regulates double-strand break processing at the repetitive ribosomal DNA array to ensure proper chromosome segregation during meiosis in budding yeast.

    abstract::During meiosis, homologues are linked by crossover, which is required for bipolar chromosome orientation before chromosome segregation at anaphase I. The repetitive ribosomal DNA (rDNA) array, however, undergoes little or no meiotic recombination. Hyperrecombination can cause chromosome missegregation and rDNA copy nu...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Li P,Jin H,Yu HG

    更新日期:2014-10-01 00:00:00

  • A mechanism of Rap1-induced stabilization of endothelial cell--cell junctions.

    abstract::Activation of Rap1 small GTPases stabilizes cell--cell junctions, and this activity requires Krev Interaction Trapped gene 1 (KRIT1). Loss of KRIT1 disrupts cardiovascular development and causes autosomal dominant familial cerebral cavernous malformations. Here we report that native KRIT1 protein binds the effector lo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Liu JJ,Stockton RA,Gingras AR,Ablooglu AJ,Han J,Bobkov AA,Ginsberg MH

    更新日期:2011-07-15 00:00:00

  • Vacuole-mediated selective regulation of TORC1-Sch9 signaling following oxidative stress.

    abstract::Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

    更新日期:2018-02-15 00:00:00

  • Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis.

    abstract::Polo-like kinase 1 (Plk1) is a key regulator of mitotic progression and cell division in eukaryotes. It is highly expressed in tumor cells and considered a potential target for cancer therapy. Here, we report the discovery and application of a novel potent small-molecule inhibitor of mammalian Plk1, ZK-Thiazolidinone ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Santamaria A,Neef R,Eberspächer U,Eis K,Husemann M,Mumberg D,Prechtl S,Schulze V,Siemeister G,Wortmann L,Barr FA,Nigg EA

    更新日期:2007-10-01 00:00:00

  • Heterogeneous nuclear ribonucleoprotein A2 is a common transcriptional coactivator in the nuclear transcription response to mitochondrial respiratory stress.

    abstract::Mitochondrial dysfunction and altered transmembrane potential initiate a mitochondrial respiratory stress response, also known as mitochondrial retrograde response, in a wide spectrum of cells. The mitochondrial stress response activates calcineurin, which regulates transcription factors, including a new nuclear facto...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Guha M,Pan H,Fang JK,Avadhani NG

    更新日期:2009-09-01 00:00:00

  • The zinc transporter ZnT3 interacts with AP-3 and it is preferentially targeted to a distinct synaptic vesicle subpopulation.

    abstract::Synaptic vesicles (SV) are generated by two different mechanisms, one AP-2 dependent and one AP-3 dependent. It has been uncertain, however, whether these mechanisms generate SV that differ in molecular composition. We explored this hypothesis by analyzing the targeting of ZnT3 and synaptophysin both to PC12 synaptic-...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Salazar G,Love R,Werner E,Doucette MM,Cheng S,Levey A,Faundez V

    更新日期:2004-02-01 00:00:00

  • Differential roles for actin polymerization and a myosin II motor in assembly of the epithelial apical junctional complex.

    abstract::Differentiation and polarization of epithelial cells depends on the formation of the apical junctional complex (AJC), which is composed of the tight junction (TJ) and the adherens junction (AJ). In this study, we investigated mechanisms of actin reorganization that drive the establishment of AJC. Using a calcium switc...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ivanov AI,Hunt D,Utech M,Nusrat A,Parkos CA

    更新日期:2005-06-01 00:00:00

  • Alpha5beta1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells.

    abstract::Cyclin D1 expression is jointly regulated by growth factors and cell adhesion to the extracellular matrix in many cell types. Growth factors are thought to regulate cyclin D1 expression because they stimulate sustained extracellular signal-regulated kinase (ERK) activity. However, we show here that growth factors indu...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Roovers K,Davey G,Zhu X,Bottazzi ME,Assoian RK

    更新日期:1999-10-01 00:00:00

  • Multivalent immune complexes divert FcRn to lysosomes by exclusion from recycling sorting tubules.

    abstract::The neonatal receptor for immunoglobulin G (IgG; FcRn) prevents IgG degradation by efficiently sorting IgG into recycling endosomes and away from lysosomes. When bound to IgG-opsonized antigen complexes, however, FcRn traffics cargo into lysosomes, where antigen processing can occur. Here we address the mechanism of s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Weflen AW,Baier N,Tang QJ,Van den Hof M,Blumberg RS,Lencer WI,Massol RH

    更新日期:2013-08-01 00:00:00

  • Delineation of glutamate pathways and secretory responses in pancreatic islets with β-cell-specific abrogation of the glutamate dehydrogenase.

    abstract::In pancreatic β-cells, glutamate dehydrogenase (GDH) modulates insulin secretion, although its function regarding specific secretagogues is unclear. This study investigated the role of GDH using a β-cell-specific GDH knockout mouse model, called βGlud1(-/-). The absence of GDH in islets isolated from βGlud1(-/-) mice ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Vetterli L,Carobbio S,Pournourmohammadi S,Martin-Del-Rio R,Skytt DM,Waagepetersen HS,Tamarit-Rodriguez J,Maechler P

    更新日期:2012-10-01 00:00:00

  • A p90(rsk) mutant constitutively interacting with MAP kinase uncouples MAP kinase from p34(cdc2)/cyclin B activation in Xenopus oocytes.

    abstract::The efficient activation of p90(rsk) by MAP kinase requires their interaction through a docking site located at the C-terminal end of p90(rsk). The MAP kinase p42(mpk1) can associate with p90(rsk) in G(2)-arrested but not in mature Xenopus oocytes. In contrast, an N-terminally truncated p90(rsk) mutant named D2 consti...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Gavin AC,Ni Ainle A,Chierici E,Jones M,Nebreda AR

    更新日期:1999-09-01 00:00:00

  • Regulation of the vitellogenin receptor during Drosophila melanogaster oogenesis.

    abstract::In many insects, development of the oocyte arrests temporarily just before vitellogenesis, the period when vitellogenins (yolk proteins) accumulate in the oocyte. Following hormonal and environmental cues, development of the oocyte resumes, and endocytosis of vitellogenins begins. An essential component of yolk uptake...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Schonbaum CP,Perrino JJ,Mahowald AP

    更新日期:2000-02-01 00:00:00

  • Role of fission yeast Tup1-like repressors and Prr1 transcription factor in response to salt stress.

    abstract::In Schizosaccharomyces pombe, the Sty1 mitogen-activated protein kinase and the Atf1 transcription factor control transcriptional induction in response to elevated salt concentrations. Herein, we demonstrate that two repressors, Tup11 and Tup12, and the Prr1 transcription factor also function in the response to salt s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Greenall A,Hadcroft AP,Malakasi P,Jones N,Morgan BA,Hoffman CS,Whitehall SK

    更新日期:2002-09-01 00:00:00

  • Incompatibility of connexin 40 and 43 Hemichannels in gap junctions between mammalian cells is determined by intracellular domains.

    abstract::Murine connexin 40 (Cx40) and connexin 43 (Cx43) do not form functional heterotypic gap junction channels. This property may contribute to the preferential propagation of action potentials in murine conductive myocardium (expressing Cx40) which is surrounded by working myocardium, expressing Cx43. When mouse Cx40 and ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Haubrich S,Schwarz HJ,Bukauskas F,Lichtenberg-Fraté H,Traub O,Weingart R,Willecke K

    更新日期:1996-12-01 00:00:00

  • Casein kinase II and calcineurin modulate TRPP function and ciliary localization.

    abstract::Cilia serve as sensory devices in a diversity of organisms and their defects contribute to many human diseases. In primary cilia of kidney cells, the transient receptor potential polycystin (TRPP) channels polycystin-1 (PC-1) and polycystin-2 (PC-2) act as a mechanosensitive channel, with defects resulting in autosoma...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Hu J,Bae YK,Knobel KM,Barr MM

    更新日期:2006-05-01 00:00:00

  • GRP78 is a novel receptor initiating a vascular barrier protective response to oxidized phospholipids.

    abstract::Vascular integrity and the maintenance of blood vessel continuity are fundamental features of the circulatory system maintained through endothelial cell-cell junctions. Defects in the endothelial barrier become an initiating factor in several pathologies, including ischemia/reperfusion, tumor angiogenesis, pulmonary e...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Birukova AA,Singleton PA,Gawlak G,Tian X,Mirzapoiazova T,Mambetsariev B,Dubrovskyi O,Oskolkova OV,Bochkov VN,Birukov KG

    更新日期:2014-07-01 00:00:00

  • Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.

    abstract::A role of Arp2/3 complex in lamellipodia is well established, whereas its roles in filopodia formation remain obscure. We addressed this question in neuronal cells, in which motility is heavily based on filopodia, and we found that Arp2/3 complex is involved in generation of both lamellipodia and filopodia in growth c...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Korobova F,Svitkina T

    更新日期:2008-04-01 00:00:00

  • A double-strand break repair component is essential for S phase completion in fission yeast cell cycling.

    abstract::Fission yeast rad22(+), a homologue of budding yeast RAD52, encodes a double-strand break repair component, which is dispensable for proliferation. We, however, have recently obtained a cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Suto K,Nagata A,Murakami H,Okayama H

    更新日期:1999-10-01 00:00:00

  • Initiation of protein synthesis by hepatitis C virus is refractory to reduced eIF2.GTP.Met-tRNA(i)(Met) ternary complex availability.

    abstract::A cornerstone of the antiviral interferon response is phosphorylation of eukaryotic initiation factor (eIF)2alpha. This limits the availability of eIF2.GTP.Met-tRNA(i)(Met) ternary complexes, reduces formation of 43S preinitiation complexes, and blocks viral (and most cellular) mRNA translation. However, many viruses ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Robert F,Kapp LD,Khan SN,Acker MG,Kolitz S,Kazemi S,Kaufman RJ,Merrick WC,Koromilas AE,Lorsch JR,Pelletier J

    更新日期:2006-11-01 00:00:00

  • TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast.

    abstract::The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential 282-kDa phosphatidylinositol kinase (PI kinase) homologue. The TOR1 gene product is also a large (281 kDa) PI kinase homol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Helliwell SB,Wagner P,Kunz J,Deuter-Reinhard M,Henriquez R,Hall MN

    更新日期:1994-01-01 00:00:00

  • Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum.

    abstract::The split-ubiquitin technique was used to detect transient protein interactions in living cells. Nub, the N-terminal half of ubiquitin (Ub), was fused to Sec62p, a component of the protein translocation machinery in the endoplasmic reticulum of Saccharomyces cerevisiae. Cub, the C-terminal half of Ub, was fused to the...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Dünnwald M,Varshavsky A,Johnsson N

    更新日期:1999-02-01 00:00:00

  • Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism.

    abstract::Transforming growth factor-beta1 (TGF-beta) can be tumor suppressive, but it can also enhance tumor progression by stimulating the complex process of epithelial-to-mesenchymal transdifferentiaion (EMT). The signaling pathway(s) that regulate EMT in response to TGF-beta are not well understood. We demonstrate the acqui...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Bhowmick NA,Ghiassi M,Bakin A,Aakre M,Lundquist CA,Engel ME,Arteaga CL,Moses HL

    更新日期:2001-01-01 00:00:00

  • Isolation of protein glycosylation mutants in the fission yeast Schizosaccharomyces pombe.

    abstract::We have isolated mutants in the fission yeast Schizosaccharomyces pombe that are defective in protein glycosylation. A collection of osmotically sensitive mutants was prepared and screened for glycosylation defects using lectin staining as an assay. Mutants singly defective in four glycoprotein synthesis genes (gps1-4...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Huang KM,Snider MD

    更新日期:1995-05-01 00:00:00