The nfkb1 promoter is controlled by proteins of the Ets family.

abstract：:Most mitochondrial proteins are synthesized as precursors that carry N-terminal presequences. After they are imported into mitochondria, these targeting signals are cleaved off by the mitochondrial processing peptidase (MPP). Using the mitochondrial tandem protein Arg5,6 as a model substrate, we demonstrate that MPP h...

journal_title：Molecular biology of the cell

authors： Friedl J,Knopp MR,Groh C,Paz E,Gould SB,Herrmann JM,Boos F

更新日期：2020-11-15 00:00:00

abstract：:14-3-3 proteins via binding serine/threonine-phosphorylated proteins regulate diverse intracellular processes in all eukaryotic organisms. Here, we examine the role of 14-3-3 self-dimerization in target binding, and in the susceptibility of 14-3-3 to undergo phosphorylation. Using a phospho-specific antibody developed...

journal_title：Molecular biology of the cell

authors： Shen YH,Godlewski J,Bronisz A,Zhu J,Comb MJ,Avruch J,Tzivion G

更新日期：2003-11-01 00:00:00

abstract：:The structural maintenance of chromosomes (SMC) protein encoded by the fission yeast rad18 gene is involved in several DNA repair processes and has an essential function in DNA replication and mitotic control. It has a heterodimeric partner SMC protein, Spr18, with which it forms the core of a multiprotein complex. We...

journal_title：Molecular biology of the cell

authors： Taylor EM,Moghraby JS,Lees JH,Smit B,Moens PB,Lehmann AR

更新日期：2001-06-01 00:00:00

abstract：:Ubiquitin-mediated proteolysis plays a key role in many pathways inside the cell and is particularly important in regulating cell cycle transitions. SCF (Skp1/Cul1/F-box protein) complexes are modular ubiquitin ligases whose specificity is determined by a substrate-binding F-box protein. Dia2 is a Saccharomyces cerevi...

journal_title：Molecular biology of the cell

authors： Koepp DM,Kile AC,Swaminathan S,Rodriguez-Rivera V

更新日期：2006-04-01 00:00:00

abstract：:Griscelli syndrome type 2 (GS2) is a genetic disorder in which patients exhibit life-threatening defects of cytotoxic T lymphocytes (CTLs) whose lytic granules fail to dock on the plasma membrane and therefore do not release their contents. The disease is caused by the absence of functional rab27a, but how rab27a cont...

journal_title：Molecular biology of the cell

authors： Neeft M,Wieffer M,de Jong AS,Negroiu G,Metz CH,van Loon A,Griffith J,Krijgsveld J,Wulffraat N,Koch H,Heck AJ,Brose N,Kleijmeer M,van der Sluijs P

更新日期：2005-02-01 00:00:00

abstract：:Up-regulation of the cAMP pathway by forskolin or alpha-melanocyte stimulating hormone induces melanocyte and melanoma cell differentiation characterized by stimulation of melanin synthesis and dendrite development. Here we show that forskolin-induced dendricity is associated to a disassembly of actin stress fibers. S...

journal_title：Molecular biology of the cell

authors： Buscà R,Bertolotto C,Abbe P,Englaro W,Ishizaki T,Narumiya S,Boquet P,Ortonne JP,Ballotti R

更新日期：1998-06-01 00:00:00

abstract：:Syntaxin 3 (Stx3), a SNARE protein located and functioning at the apical plasma membrane of epithelial cells, is required for epithelial polarity. A fraction of Stx3 is localized to late endosomes/lysosomes, although how it traffics there and its function in these organelles is unknown. Here we report that Stx3 underg...

journal_title：Molecular biology of the cell

authors： Giovannone AJ,Reales E,Bhattaram P,Fraile-Ramos A,Weimbs T

更新日期：2017-10-15 00:00:00

abstract：:Although p38 activity is reported to be required as cells enter mitosis for proper spindle assembly and checkpoint function, its role during the division process remains controversial in lieu of direct data. We therefore conducted live cell studies to determine the effect on mitosis of inhibiting or depleting p38. We ...

journal_title：Molecular biology of the cell

authors： Lee K,Kenny AE,Rieder CL

更新日期：2010-07-01 00:00:00

abstract：:In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significan...

journal_title：Molecular biology of the cell

authors： Turowski P,Franckhauser C,Morris MC,Vaglio P,Fernandez A,Lamb NJ

更新日期：2003-07-01 00:00:00

abstract：:Gaucher disease is a lysosomal storage disorder caused by a defect in the degradation of glucosylceramide catalyzed by the lysosomal enzyme β-glucocerebrosidase (GBA). GBA reaches lysosomes via association with its receptor, lysosomal integral membrane protein type 2 (LIMP-2). We found that distinct phosphatidylinosit...

journal_title：Molecular biology of the cell

authors： Jović M,Kean MJ,Szentpetery Z,Polevoy G,Gingras AC,Brill JA,Balla T

更新日期：2012-04-01 00:00:00

abstract：:What started as a game of discovery for a child with a chemistry set has resulted in a lifetime of fulfillment and dedication to science. It is deeply rewarding to continuously let my curiosity ask questions for which there are no known answers. Equally rewarding has been the opportunity to mentor young students and p...

journal_title：Molecular biology of the cell

authors： Garcia JV

更新日期：2013-11-01 00:00:00

abstract：:When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins a...

journal_title：Molecular biology of the cell

authors： González-Novo A,Correa-Bordes J,Labrador L,Sánchez M,Vázquez de Aldana CR,Jiménez J

更新日期：2008-04-01 00:00:00

abstract：:ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response (UPR), which up-regulates genes that enable cells to cope with misfolded, ER-retained proteins. In this study, we compare th...

journal_title：Molecular biology of the cell

authors： Metzger MB,Michaelis S

更新日期：2009-02-01 00:00:00

abstract：:Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. Claspin associates with replication forks upon origin unwinding. We show that Claspin contains a replication fork-interacting domain (RFID, residues 265-605) that associates with Cdc45, DNA pol...

journal_title：Molecular biology of the cell

authors： Lee J,Gold DA,Shevchenko A,Shevchenko A,Dunphy WG

更新日期：2005-11-01 00:00:00

abstract：:Protein kinase C (PKC) zeta has been implicated in insulin-induced glucose uptake in skeletal muscle cell, although the underlying mechanism remains unknown. In this study, we investigated the effect of PKCzeta on actin remodeling and glucose transport in differentiated rat L6 muscle cells expressing myc-tagged glucos...

journal_title：Molecular biology of the cell

authors： Liu LZ,Zhao HL,Zuo J,Ho SK,Chan JC,Meng Y,Fang FD,Tong PC

更新日期：2006-05-01 00:00:00

abstract：:Directed cell growth or migration are critical for the development and function of many eukaryotic cells. These cells develop a dynamic "front" (also called "polarity site") that can change direction. Polarity establishment involves autocatalytic accumulation of polarity regulators, including the conserved Rho-family ...

journal_title：Molecular biology of the cell

authors： Ghose D,Lew D

更新日期：2020-05-01 00:00:00

abstract：:Recent evidence has shown that members of the Jak kinase family are activated after IL-6 binds to its receptor complex, leading to a tyrosine phosphorylation of gp130, the IL-6 signal-transducing subunit. The different members of the IL-6 cytokine subfamily induce distinct patterns of Jak-Tyk phosphorylation in differ...

journal_title：Molecular biology of the cell

authors： Wang Y,Fuller GM

更新日期：1994-07-01 00:00:00

abstract：:Metabolic adaptation, and in particular the modulation of carbon assimilatory pathways during disease progression, is thought to contribute to the pathogenicity of Candida albicans. Therefore, we have examined the global impact of glucose upon the C. albicans transcriptome, testing the sensitivity of this pathogen to ...

journal_title：Molecular biology of the cell

authors： Rodaki A,Bohovych IM,Enjalbert B,Young T,Odds FC,Gow NA,Brown AJ

更新日期：2009-11-01 00:00:00

abstract：:Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of i...

journal_title：Molecular biology of the cell

authors： Shekhar S,Carlier MF

更新日期：2016-01-01 00:00:00

abstract：:Tetraploidization, or genome doubling, is a prominent event in tumorigenesis, primarily because cell division in polyploid cells is error-prone and produces aneuploid cells. This study investigates changes in gene expression evoked in acute and adapted tetraploid cells and their effect on cell-cycle progression. Acute...

journal_title：Molecular biology of the cell

authors： Potapova TA,Seidel CW,Box AC,Rancati G,Li R

更新日期：2016-10-15 00:00:00

abstract：:In neurons certain mRNA transcripts are transported to synapses through mechanisms that are not fully understood. Here we report that the heterogeneous nuclear ribonucleoprotein CBF-A (CArG Box binding Factor A) facilitates dendritic transport and localization of activity-regulated cytoskeleton-associated protein (Arc...

journal_title：Molecular biology of the cell

authors： Raju CS,Fukuda N,López-Iglesias C,Göritz C,Visa N,Percipalle P

更新日期：2011-06-01 00:00:00

abstract：:Pif1 family helicases, which are found in nearly all eukaryotes, have important roles in both nuclear and mitochondrial genome maintenance. Recently, the increasing availability of genome sequences has revealed that Pif1 helicases are also widely found in diverse prokaryotes, but it is currently unknown what physiolog...

journal_title：Molecular biology of the cell

authors： Bochman ML,Judge CP,Zakian VA

更新日期：2011-06-15 00:00:00

abstract：:During constitutive secretion, proteins synthesized at the endoplasmic reticulum (ER) are transported to the Golgi complex for processing and then to the plasma membrane for incorporation or extracellular release. This study uses a unique live-cell constitutive secretion assay to establish roles for the molecular moto...

journal_title：Molecular biology of the cell

authors： Bond LM,Peden AA,Kendrick-Jones J,Sellers JR,Buss F

更新日期：2011-01-01 00:00:00

abstract：:A key question in eukaryotic differentiation is whether there are common regulators or biochemical events that are required for diverse types of differentiation or whether there is a core mechanism for differentiation. The unicellular model organism Saccharomyces cerevisiae undergoes filamentous differentiation in res...

journal_title：Molecular biology of the cell

authors： Jiang YW,Kang CM

更新日期：2003-12-01 00:00:00

abstract：:The gamma-tubulin small complex (gamma-TuSC) is an evolutionarily conserved heterotetramer essential for microtubule nucleation. We have determined the structure of the Saccharomyces cerevisiae gamma-TuSC at 25-A resolution by electron microscopy. gamma-TuSC is Y-shaped, with an elongated body connected to two arms. G...

journal_title：Molecular biology of the cell

authors： Kollman JM,Zelter A,Muller EG,Fox B,Rice LM,Davis TN,Agard DA

更新日期：2008-01-01 00:00:00

abstract：:Many cellular processes change during the Trypanosoma brucei life cycle as this parasite alternates between the mammalian host and tsetse fly vector. We show that the inositol phosphate pathway helps regulate these developmental changes. Knockdown of inositol polyphosphate multikinase (IPMK), which phosphorylates Ins(...

journal_title：Molecular biology of the cell

authors： Cestari I,Anupama A,Stuart K

更新日期：2018-05-01 00:00:00

abstract：:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERd...

journal_title：Molecular biology of the cell

authors： Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TE

更新日期：2014-02-01 00:00:00

abstract：:NSF and p97 are related AAA proteins implicated in membrane trafficking and organelle biogenesis. p97 is also involved in pathways that lead to ubiquitin-dependent proteolysis, including ER-associated degradation (ERAD). In this study, we have used dominant interfering ATP-hydrolysis deficient mutants (NSF(E329Q) and ...

journal_title：Molecular biology of the cell

authors： Dalal S,Rosser MF,Cyr DM,Hanson PI

更新日期：2004-02-01 00:00:00

abstract：:Most membrane proteins are synthesized on and inserted into the membrane of the endoplasmic reticulum (ER), in eukaryote. The widely conserved ER membrane protein complex (EMC) facilitates the biogenesis of a wide range of membrane proteins. In this study, we investigated the EMC function using Drosophila photorecepto...

journal_title：Molecular biology of the cell

authors： Hiramatsu N,Tago T,Satoh T,Satoh AK

更新日期：2019-11-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00