Inhibition of Rho is required for cAMP-induced melanoma cell differentiation.

abstract：:Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian...

journal_title：Molecular biology of the cell

authors： Ruepp MD,Aringhieri C,Vivarelli S,Cardinale S,Paro S,Schümperli D,Barabino SM

更新日期：2009-12-01 00:00:00

abstract：:In Tetrahymena thermophila, peptides secreted via dense-core granules, called mucocysts, are generated by proprotein processing. We used expression profiling to identify candidate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts. Of note, the aspartyl cathepsin Cth3p plays a key rol...

journal_title：Molecular biology of the cell

authors： Kumar S,Briguglio JS,Turkewitz AP

更新日期：2014-08-15 00:00:00

abstract：:Vascular integrity and the maintenance of blood vessel continuity are fundamental features of the circulatory system maintained through endothelial cell-cell junctions. Defects in the endothelial barrier become an initiating factor in several pathologies, including ischemia/reperfusion, tumor angiogenesis, pulmonary e...

journal_title：Molecular biology of the cell

authors： Birukova AA,Singleton PA,Gawlak G,Tian X,Mirzapoiazova T,Mambetsariev B,Dubrovskyi O,Oskolkova OV,Bochkov VN,Birukov KG

更新日期：2014-07-01 00:00:00

abstract：:In eukaryotic cells, organelle-specific protein quality control (PQC) is critical for maintaining cellular homeostasis. Despite the Golgi apparatus being the major protein processing and sorting site within the secretory pathway, how it contributes to PQC has remained largely unknown. Using different chemical biology-...

journal_title：Molecular biology of the cell

authors： Hellerschmied D,Serebrenik YV,Shao L,Burslem GM,Crews CM

更新日期：2019-08-01 00:00:00

abstract：:We observed the dynamic recruitment of spindle checkpoint proteins Mad1 and Bub1 to detached kinetochores in budding yeast using real-time live-cell imaging and quantified recruitment in fixed cells. After induced de novo kinetochore assembly at one pair of sister centromeres, Mad1 appeared after the kinetochore prote...

journal_title：Molecular biology of the cell

authors： Krefman NI,Drubin DG,Barnes G

更新日期：2015-07-15 00:00:00

abstract：:The tumor suppressor protein p53 mediates stress-induced growth arrest or apoptosis and plays a major role in safeguarding genome integrity. In response to DNA damage, p53 can be modified at multiple sites by phosphorylation and acetylation. We report on the characterization of p53 C-terminal phosphorylation by CHK1 a...

journal_title：Molecular biology of the cell

authors： Ou YH,Chung PH,Sun TP,Shieh SY

更新日期：2005-04-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the Apg12p-Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2 mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this a...

journal_title：Molecular biology of the cell

authors： Tanida I,Mizushima N,Kiyooka M,Ohsumi M,Ueno T,Ohsumi Y,Kominami E

更新日期：1999-05-01 00:00:00

abstract：:Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appear...

journal_title：Molecular biology of the cell

authors： Lo KY,Johnson AW

更新日期：2009-03-01 00:00:00

abstract：:Various human diseases are associated with mitochondrial DNA (mtDNA) mutations, but heteroplasmy—the coexistence of mutant and wild-type mtDNA—complicates their study. We previously isolated a temperature-lethal mtDNA mutation in Drosophila, mt:CoI(T300I), which affects the cytochrome c oxidase subunit I (CoI) locus. ...

journal_title：Molecular biology of the cell

authors： Chen Z,Qi Y,French S,Zhang G,Covian Garcia R,Balaban R,Xu H

更新日期：2015-02-15 00:00:00

abstract：:Organelle inheritance occurs during cell division. In Saccharomyces cerevisiae, inheritance of the vacuole, and the distribution of mitochondria and cortical endoplasmic reticulum are regulated by Ptc1p, a type 2C protein phosphatase. Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved b...

journal_title：Molecular biology of the cell

authors： Jin Y,Taylor Eves P,Tang F,Weisman LS

更新日期：2009-03-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...

journal_title：Molecular biology of the cell

authors： Deaton JD,Guerrero T,Howard TH

更新日期：1992-12-01 00:00:00

abstract：:We report a new nuclear gene, designated ATP25 (reading frame YMR098C on chromosome XIII), required for expression of Atp9p (subunit 9) of the Saccharomyces cerevisiae mitochondrial proton translocating ATPase. Mutations in ATP25 elicit a deficit of ATP9 mRNA and of its translation product, thereby preventing assembly...

journal_title：Molecular biology of the cell

authors： Zeng X,Barros MH,Shulman T,Tzagoloff A

更新日期：2008-04-01 00:00:00

abstract：:We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Se...

journal_title：Molecular biology of the cell

authors： Denegri M,Moralli D,Rocchi M,Biggiogera M,Raimondi E,Cobianchi F,De Carli L,Riva S,Biamonti G

更新日期：2002-06-01 00:00:00

abstract：:The target of rapamycin kinase complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. In Saccharomyces cerevisiae, TORC1 activity is known to be controlled by the conserved GTPases, Gtr1/2, and movement into and out of an inactive agglomerate/body. However, it is unclear whether/how these regulatory ste...

journal_title：Molecular biology of the cell

authors： Sullivan A,Wallace RL,Wellington R,Luo X,Capaldi AP

更新日期：2019-02-01 00:00:00

abstract：:Sorting nexin 1 (SNX1) and SNX2, homologues of the yeast vacuolar protein-sorting (Vps)5p, contain a phospholipid-binding motif termed the phox homology (PX) domain and a carboxyl terminal coiled-coil region. A role for SNX1 in trafficking of cell surface receptors from endosomes to lysosomes has been proposed; howeve...

journal_title：Molecular biology of the cell

authors： Gullapalli A,Garrett TA,Paing MM,Griffin CT,Yang Y,Trejo J

更新日期：2004-05-01 00:00:00

abstract：:The serotonin (5-hydroxytryptamine; 5-HT)(2C) receptor is a G protein-coupled receptor (GPCR) exclusively expressed in CNS that has been implicated in numerous brain disorders, including anxio-depressive states. Like many GPCRs, 5-HT(2C) receptors physically interact with a variety of intracellular proteins in additio...

journal_title：Molecular biology of the cell

authors： Labasque M,Reiter E,Becamel C,Bockaert J,Marin P

更新日期：2008-11-01 00:00:00

abstract：:Functionally different subsets of actin filament arrays contribute to cellular organization and motility. We report the identification of a novel subset of loose actin filament arrays through regulated association with the widely expressed protein SWAP-70. These loose actin filament arrays were commonly located behind...

journal_title：Molecular biology of the cell

authors： Hilpelä P,Oberbanscheidt P,Hahne P,Hund M,Kalhammer G,Small JV,Bähler M

更新日期：2003-08-01 00:00:00

abstract：:We have identified a novel Ras-interacting protein from Dictyostelium, RIP3, whose function is required for both chemotaxis and the synthesis and relay of the cyclic AMP (cAMP) chemoattractant signal. rip3 null cells are unable to aggregate and lack receptor activation of adenylyl cyclase but are able, in response to ...

journal_title：Molecular biology of the cell

authors： Lee S,Parent CA,Insall R,Firtel RA

更新日期：1999-09-01 00:00:00

abstract：:Multisite phosphorylation of proteins is a powerful signal processing mechanism that plays crucial roles in cell division and differentiation as well as in disease. We recently demonstrated a novel phenomenon in cell cycle regulation by showing that cyclin-dependent kinase-dependent multisite phosphorylation of a cruc...

journal_title：Molecular biology of the cell

authors： Valk E,Venta R,Ord M,Faustova I,Kõivomägi M,Loog M

更新日期：2014-11-05 00:00:00

abstract：:Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome...

journal_title：Molecular biology of the cell

authors： Hnilicová J,Hozeifi S,Stejskalová E,Dušková E,Poser I,Humpolíčková J,Hof M,Staněk D

更新日期：2013-11-01 00:00:00

abstract：:The intermembrane space of mitochondria accommodates the essential mitochondrial intermembrane space assembly (MIA) machinery that catalyzes oxidative folding of proteins. The disulfide bond formation pathway is based on a relay of reactions involving disulfide transfer from the sulfhydryl oxidase Erv1 to Mia40 and fr...

journal_title：Molecular biology of the cell

authors： Böttinger L,Gornicka A,Czerwik T,Bragoszewski P,Loniewska-Lwowska A,Schulze-Specking A,Truscott KN,Guiard B,Milenkovic D,Chacinska A

更新日期：2012-10-01 00:00:00

abstract：:Kinetochores are the proteinaceous complexes that assemble on centromeric DNA and direct eukaryotic chromosome segregation. The mechanisms by which higher eukaryotic cells define centromeres are poorly understood. Possible molecular contributors to centromere specification include the underlying DNA sequences and epig...

journal_title：Molecular biology of the cell

authors： Edwards NS,Murray AW

更新日期：2005-04-01 00:00:00

abstract：:A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of th...

journal_title：Molecular biology of the cell

authors： Midwood KS,Schwarzbauer JE

更新日期：2002-10-01 00:00:00

abstract：:Starvation induces Dictyostelium amoebae to secrete cAMP, toward which other amoebae stream, forming multicellular mounds that differentiate and develop into fruiting bodies containing spores. We find that the double deletion of cortexillin (ctx) I and II alters the actin cytoskeleton and substantially inhibits all mo...

journal_title：Molecular biology of the cell

authors： Shu S,Liu X,Kriebel PW,Daniels MP,Korn ED

更新日期：2012-01-01 00:00:00

abstract：:We have identified and characterized sorb-1, the only sorbin and SH3 domain-containing protein family member in Caenorhabditis elegans SORB-1 is strongly localized to integrin adhesion complexes in larvae and adults, including adhesion plaques and dense bodies (Z-disks) of striated muscles and attachment plaques of sm...

journal_title：Molecular biology of the cell

authors： Loveless T,Qadota H,Benian GM,Hardin J

更新日期：2017-12-01 00:00:00

abstract：:B cell antigen receptor (BCR) association with lipid rafts, the actin cytoskeleton, and clathrin-coated pits influences B cell signaling and antigen presentation. Although all three cellular structures have been separately implicated in BCR internalization, the relationship between them has not been clearly defined. I...

journal_title：Molecular biology of the cell

authors： Stoddart A,Jackson AP,Brodsky FM

更新日期：2005-05-01 00:00:00

abstract：:The NUDF protein of the filamentous fungus Aspergillus nidulans functions in the cytoplasmic dynein pathway. It binds several proteins, including the NUDE protein. Green fluorescent protein-tagged NUDF and NUDA (dynein heavy chain) localize to linearly moving dashes ("comets") that coincide with microtubule ends. Here...

journal_title：Molecular biology of the cell

authors： Efimov VP

更新日期：2003-03-01 00:00:00

abstract：:Multiple tau gene mutations are pathogenic for hereditary frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), with filamentous tau aggregates as the major lesions in the CNS of these patients. Recent studies have shown that bacterially expressed recombinant tau proteins with FTDP-17 missense mu...

journal_title：Molecular biology of the cell

authors： Vogelsberg-Ragaglia V,Bruce J,Richter-Landsberg C,Zhang B,Hong M,Trojanowski JQ,Lee VM

更新日期：2000-12-01 00:00:00

abstract：:The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin...

journal_title：Molecular biology of the cell

authors： Shimi T,Kittisopikul M,Tran J,Goldman AE,Adam SA,Zheng Y,Jaqaman K,Goldman RD

更新日期：2015-11-05 00:00:00