PTC1 is required for vacuole inheritance and promotes the association of the myosin-V vacuole-specific receptor complex.

abstract：:We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same me...

journal_title：Molecular biology of the cell

authors： Saldanha AJ,Brauer MJ,Botstein D

更新日期：2004-09-01 00:00:00

abstract：:Thrombospondin (TSP) was demonstrated to inhibit the growth of bovine aortic endothelial cells, an activity that was not neutralized by antibodies to TSP or by other agents that block TSP-cell interactions but that partially was reversed by a neutralizing antibody to transforming growth factor-beta (TGF-beta). Similar...

journal_title：Molecular biology of the cell

authors： Murphy-Ullrich JE,Schultz-Cherry S,Höök M

更新日期：1992-02-01 00:00:00

abstract：:The structural maintenance of chromosomes (SMC) protein encoded by the fission yeast rad18 gene is involved in several DNA repair processes and has an essential function in DNA replication and mitotic control. It has a heterodimeric partner SMC protein, Spr18, with which it forms the core of a multiprotein complex. We...

journal_title：Molecular biology of the cell

authors： Taylor EM,Moghraby JS,Lees JH,Smit B,Moens PB,Lehmann AR

更新日期：2001-06-01 00:00:00

abstract：:Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides d...

journal_title：Molecular biology of the cell

authors： Verbsky JW,Turkewitz AP

更新日期：1998-02-01 00:00:00

abstract：:cAR1, a G protein-coupled receptor (GPCR) for cAMP, is required for the multicellular development of Dictyostelium. The activation of multiple pathways by cAR1 is transient because of poorly defined adaptation mechanisms. To investigate this, we used a genetic screen for impaired development to isolate four dominant-n...

journal_title：Molecular biology of the cell

authors： Zhang M,Goswami M,Hereld D

更新日期：2005-02-01 00:00:00

abstract：:m-AAA proteases are ATP-dependent proteolytic machines in the inner membrane of mitochondria which are crucial for the maintenance of mitochondrial activities. Conserved nuclear-encoded subunits, termed paraplegin, Afg3l1, and Afg3l2, form various isoenzymes differing in their subunit composition in mammalian mitochon...

journal_title：Molecular biology of the cell

authors： Koppen M,Bonn F,Ehses S,Langer T

更新日期：2009-10-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts wit...

journal_title：Molecular biology of the cell

authors： Newpher TM,Idrissi FZ,Geli MI,Lemmon SK

更新日期：2006-10-01 00:00:00

abstract：:The majority of mitochondrial phosphatidylethanolamine (PtdEtn), a phospholipid essential for aerobic growth of yeast cells, is synthesized by phosphatidylserine decarboxylase 1 (Psd1p) in the inner mitochondrial membrane (IMM). To identify components that become essential when the level of mitochondrial PtdEtn is dec...

journal_title：Molecular biology of the cell

authors： Birner R,Nebauer R,Schneiter R,Daum G

更新日期：2003-02-01 00:00:00

abstract：:Bile ducts are hepatic tubular structures that are lined by cholangiocytes, a type of liver epithelial cell. Cholangiocytes first form a single layer of cells, termed the ductal plate, surrounding the portal vein, which eventually remodels into the branching tubular network of bile ducts. The process of bile duct morp...

journal_title：Molecular biology of the cell

authors： Tanimizu N,Miyajima A,Mostov KE

更新日期：2009-05-01 00:00:00

abstract：:Rab8 is a GTPase involved in membrane trafficking. In photoreceptor cells, rab8 is proposed to participate in the late stages of delivery of rhodopsin-containing post-Golgi membranes to the plasma membrane near the base of the connecting cilium. To test the function of rab8 in vivo, we generated transgenic Xenopus lae...

journal_title：Molecular biology of the cell

authors： Moritz OL,Tam BM,Hurd LL,Peränen J,Deretic D,Papermaster DS

更新日期：2001-08-01 00:00:00

abstract：:In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuol...

journal_title：Molecular biology of the cell

authors： Kabeya Y,Kamada Y,Baba M,Takikawa H,Sasaki M,Ohsumi Y

更新日期：2005-05-01 00:00:00

abstract：:The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]....

journal_title：Molecular biology of the cell

authors： Kryndushkin D,Wickner RB

更新日期：2007-06-01 00:00:00

abstract：:Glucose is a master regulator of cell behavior in the yeast Saccharomyces cerevisiae. It acts as both a metabolic substrate and a potent regulator of intracellular signaling cascades. Glucose starvation induces the transient delocalization and then partial relocalization of clathrin adaptors at the trans-Golgi network...

journal_title：Molecular biology of the cell

authors： Aoh QL,Hung CW,Duncan MC

更新日期：2013-03-01 00:00:00

abstract：:ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response (UPR), which up-regulates genes that enable cells to cope with misfolded, ER-retained proteins. In this study, we compare th...

journal_title：Molecular biology of the cell

authors： Metzger MB,Michaelis S

更新日期：2009-02-01 00:00:00

abstract：:Of the uncloned ODA genes required for outer dynein arm assembly in Chlamydomonas, ODA5 and ODA10 are of particular interest because they do not encode known subunits of the outer arm or the outer dynein arm-docking complex (ODA-DC), and because genetic studies suggest their products interact. Beginning with a tagged ...

journal_title：Molecular biology of the cell

authors： Wirschell M,Pazour G,Yoda A,Hirono M,Kamiya R,Witman GB

更新日期：2004-06-01 00:00:00

abstract：:Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that Numb functions in clathrin-dependent endocytosis by binding to the AP-2 complex. Numb is also expressed in postmitotic neur...

journal_title：Molecular biology of the cell

authors： Nishimura T,Yamaguchi T,Tokunaga A,Hara A,Hamaguchi T,Kato K,Iwamatsu A,Okano H,Kaibuchi K

更新日期：2006-03-01 00:00:00

abstract：:Despite the importance of epithelial cell contacts in determining cell behavior, we still lack a detailed understanding of the assembly and disassembly of intercellular contacts. Here we examined the role of the catalytic activity of the Src family kinases at epithelial cell contacts in vitro. Like E- and P-cadherin, ...

journal_title：Molecular biology of the cell

authors： Owens DW,McLean GW,Wyke AW,Paraskeva C,Parkinson EK,Frame MC,Brunton VG

更新日期：2000-01-01 00:00:00

abstract：:Outer dynein arms (ODAs) are multiprotein complexes that drive flagellar beating. Based on genetic and biochemical analyses, ODAs preassemble in the cell body and then move into the flagellum by intraflagellar transport (IFT). To study ODA transport in vivo, we expressed the essential intermediate chain 2 tagged with ...

journal_title：Molecular biology of the cell

authors： Dai J,Barbieri F,Mitchell DR,Lechtreck KF

更新日期：2018-10-15 00:00:00

abstract：:SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosph...

journal_title：Molecular biology of the cell

authors： Sakurai C,Itakura M,Kinoshita D,Arai S,Hashimoto H,Wada I,Hatsuzawa K

更新日期：2018-07-15 00:00:00

abstract：:Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

journal_title：Molecular biology of the cell

authors： Bendezú FO,Martin SG

更新日期：2011-01-01 00:00:00

abstract：:Tumor initiation and growth is associated with significant changes in the surrounding tissue. During carcinoma progression, a global stiffening of the extracellular matrix is observed and is interpreted as a signature of aggressive invasive tumors. However, it is still unknown whether this increase in matrix rigidity ...

journal_title：Molecular biology of the cell

authors： Staneva R,Burla F,Koenderink GH,Descroix S,Vignjevic DM,Attieh Y,Verhulsel M

更新日期：2018-12-01 00:00:00

abstract：:Notch and neurotrophins control neuronal shape, but it is not known whether their signaling pathways intersect. Here we report results from hippocampal neuronal cultures that are in support of this possibility. We found that low cell density or blockade of Notch signaling by a soluble Delta-Fc ligand decreased the mRN...

journal_title：Molecular biology of the cell

authors： Salama-Cohen P,Arévalo MA,Meier J,Grantyn R,Rodríguez-Tébar A

更新日期：2005-01-01 00:00:00

abstract：:Mechanically stressed cells display increased levels of fos message and protein. Although the intracellular signaling pathways responsible for FOS induction have been extensively characterized, we still do not understand the nature of the primary cell mechanotransduction event responsible for converting an externally ...

journal_title：Molecular biology of the cell

authors： Grembowicz KP,Sprague D,McNeil PL

更新日期：1999-04-01 00:00:00

abstract：:In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step in sterol biosynthesis. We have investigated the ...

journal_title：Molecular biology of the cell

authors： Koning AJ,Roberts CJ,Wright RL

更新日期：1996-05-01 00:00:00

abstract：:Cell migration is a highly conserved process involving cytoskeletal reorganization and restructuring of the surrounding extracellular matrix. Although there are many studies describing mechanisms underlying cell motility, little has been reported about the contribution of alternative isoform use toward cell migration....

journal_title：Molecular biology of the cell

authors： Lee HN,Mitra M,Bosompra O,Corney DC,Johnson EL,Rashed N,Ho LD,Coller HA

更新日期：2018-08-01 00:00:00

abstract：:Proteins that directly regulate tumor necrosis factor (TNF) signaling have critical roles in determining cell death and survival. Previously we characterized ubiquitously expressed transcript (UXT)-V2 as a novel transcriptional cofactor to regulate nuclear factor-κB in the nucleus. Here we report that another splicing...

journal_title：Molecular biology of the cell

authors： Huang Y,Chen L,Zhou Y,Liu H,Yang J,Liu Z,Wang C

更新日期：2011-04-15 00:00:00

abstract：:An increase in the level of active, GTP-bound Ras is not necessary for transformation of chicken embryo fibroblasts (CEF) by v-Src. This suggests that other Ras-independent pathways contribute to transformation by v-Src. To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammali...

journal_title：Molecular biology of the cell

authors： Penuel E,Martin GS

更新日期：1999-06-01 00:00:00

abstract：:Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carbo...

journal_title：Molecular biology of the cell

authors： Wang PC,Vancura A,Mitcheson TG,Kuret J

更新日期：1992-03-01 00:00:00

abstract：:In complex with FKBP12, the immunosuppressant rapamycin binds to and inhibits the yeast TOR1 and TOR2 proteins and the mammalian homologue mTOR/FRAP/RAFT1. The TOR proteins promote cell cycle progression in yeast and human cells by regulating translation and polarization of the actin cytoskeleton. A C-terminal domain ...

journal_title：Molecular biology of the cell

authors： Alarcon CM,Heitman J,Cardenas ME

更新日期：1999-08-01 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00