Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export.

abstract：:Motile cells such as bacteria, amoebae, and fibroblasts display a continual level of energy-consuming reactions involving the cytoskeleton and signal pathways, regardless of whether or not they are actually migrating. I draw parallels between these "silent signals" and the intrinsic activity of the human brain, especi...

journal_title：Molecular biology of the cell

authors： Bray D

更新日期：2014-03-01 00:00:00

abstract：:SPOP, speckle-type POZ protein is a substrate adaptor protein of the Cullin-3/RING ubiquitin E3 complex. The spop gene is the most commonly point mutated in human primary prostate cancers, but the pathological contribution of the SPOP mutations remains unclear. In this study, we investigated several known factors that...

journal_title：Molecular biology of the cell

authors： Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

更新日期：2020-03-15 00:00:00

abstract：:The export of mRNA from the nucleus to the cytoplasm involves interactions of proteins with mRNA and the nuclear pore complex. We isolated Crp79p, a novel mRNA export factor from the same synthetic lethal screen that led to the identification of spMex67p in Schizosaccharomyces pombe. Crp79p is a 710-amino-acid-long pr...

journal_title：Molecular biology of the cell

authors： Thakurta AG,Whalen WA,Yoon JH,Bharathi A,Kozak L,Whiteford C,Love DC,Hanover JA,Dhar R

更新日期：2002-08-01 00:00:00

abstract：:We applied a combination of laser microsurgery and quantitative polarization microscopy to study kinetochore-independent forces that act on chromosome arms during meiosis in crane fly spermatocytes. When chromosome arms located within one of the half-spindles during prometa- or metaphase were cut with the laser, the a...

journal_title：Molecular biology of the cell

authors： LaFountain JR Jr,Oldenbourg R,Cole RW,Rieder CL

更新日期：2001-12-01 00:00:00

abstract：:The neonatal receptor for immunoglobulin G (IgG; FcRn) prevents IgG degradation by efficiently sorting IgG into recycling endosomes and away from lysosomes. When bound to IgG-opsonized antigen complexes, however, FcRn traffics cargo into lysosomes, where antigen processing can occur. Here we address the mechanism of s...

journal_title：Molecular biology of the cell

authors： Weflen AW,Baier N,Tang QJ,Van den Hof M,Blumberg RS,Lencer WI,Massol RH

更新日期：2013-08-01 00:00:00

abstract：:Calreticulin (CRT) is a high-capacity, low-affinity Ca2+-binding protein located in the lumen of the endoplasmic reticulum (ER) of all eukaryotic cells investigated so far. Its high level of conservation among different species suggests that it serves functions fundamental to cell survival. The role originally propose...

journal_title：Molecular biology of the cell

authors： Fasolato C,Pizzo P,Pozzan T

更新日期：1998-06-01 00:00:00

abstract：:Up-regulation of the cytoskeleton linker protein ezrin frequently occurs in aggressive cancer types and is closely linked with metastatic progression. However, the underlying molecular mechanisms detailing how ezrin is involved in the invasive and metastatic phenotype remain unclear. Here we report a novel function of...

journal_title：Molecular biology of the cell

authors： Hoskin V,Szeto A,Ghaffari A,Greer PA,Côté GP,Elliott BE

更新日期：2015-10-01 00:00:00

abstract：:During differentiation, keratinocytes undergo a dramatic shape change from small and round to large and flat, in addition to production of proteins necessary for the formation of epidermis. It has been shown that protein kinase C (PKC) η is crucial for keratinocyte differentiation. However, its role in this process ha...

journal_title：Molecular biology of the cell

authors： Shirai Y,Morioka S,Sakuma M,Yoshino K,Otsuji C,Sakai N,Kashiwagi K,Chida K,Shirakawa R,Horiuchi H,Nishigori C,Ueyama T,Saito N

更新日期：2011-04-15 00:00:00

abstract：:In this study, we investigated the role of phospholipase D (PLD) in mediating Arf6 function in cells. Expression of Arf6 mutants that are defective in activating PLD, Arf6N48R and Arf6N48I, inhibited membrane recycling to the plasma membrane (PM), resulting in an accumulation of tubular endosomal membranes. Additional...

journal_title：Molecular biology of the cell

authors： Jovanovic OA,Brown FD,Donaldson JG

更新日期：2006-01-01 00:00:00

abstract：:The juvenile form of neuronal ceroid lipofuscinoses (JNCLs), or Batten disease, results from mutations in the CLN3 gene, and it is characterized by the accumulation of lipopigments in the lysosomes of several cell types and by extensive neuronal death. We report that the yeast model for JNCL (btn1-Delta) that lacks BT...

journal_title：Molecular biology of the cell

authors： Osório NS,Carvalho A,Almeida AJ,Padilla-Lopez S,Leão C,Laranjinha J,Ludovico P,Pearce DA,Rodrigues F

更新日期：2007-07-01 00:00:00

abstract：:The Notch signaling pathway plays essential roles in both animal development and human disease. Regulation of Notch receptor levels in membrane compartments has been shown to affect signaling in a variety of contexts. Here we used steady-state and pulse-labeling techniques to follow Notch receptors in sensory organ pr...

journal_title：Molecular biology of the cell

authors： Johnson SA,Zitserman D,Roegiers F

更新日期：2016-09-15 00:00:00

abstract：:The skin's epidermis is a multilayered epithelial tissue and the first line of defense against mechanical stress. Its barrier function depends on an integrated assembly and reorganization of cell-matrix and cell-cell junctions in the basal layer and on different intercellular junctions in suprabasal layers. However, h...

journal_title：Molecular biology of the cell

authors： Noethel B,Ramms L,Dreissen G,Hoffmann M,Springer R,Rübsam M,Ziegler WH,Niessen CM,Merkel R,Hoffmann B

更新日期：2018-09-15 00:00:00

abstract：:Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

journal_title：Molecular biology of the cell

authors： Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

更新日期：2010-08-15 00:00:00

abstract：:Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell-cell contacts. We investigated the changes in focal adhesion proteins during etoposide-induced apoptosis in Rat-1 cells and found that during apoptosis, p130cas (...

journal_title：Molecular biology of the cell

authors： Kook S,Shim SR,Choi SJ,Ahnn J,Kim JI,Eom SH,Jung YK,Paik SG,Song WK

更新日期：2000-03-01 00:00:00

abstract：:Phospholipase Ds (PLDs) are regulated enzymes that generate phosphatidic acid (PA), a putative second messenger implicated in the regulation of vesicular trafficking and cytoskeletal reorganization. Mast cells, when stimulated with antigen, show a dramatic alteration in their cytoskeleton and also release their secret...

journal_title：Molecular biology of the cell

authors： O'Luanaigh N,Pardo R,Fensome A,Allen-Baume V,Jones D,Holt MR,Cockcroft S

更新日期：2002-10-01 00:00:00

abstract：:Trimming of N-linked oligosaccharides by endoplasmic reticulum (ER) glucosidase II is implicated in quality control of protein folding. An alternate glucosidase II-independent deglucosylation pathway exists, in which endo-alpha-mannosidase cleaves internally the glucose-substituted mannose residue of oligosaccharides....

journal_title：Molecular biology of the cell

authors： Zuber C,Spiro MJ,Guhl B,Spiro RG,Roth J

更新日期：2000-12-01 00:00:00

abstract：:The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential 282-kDa phosphatidylinositol kinase (PI kinase) homologue. The TOR1 gene product is also a large (281 kDa) PI kinase homol...

journal_title：Molecular biology of the cell

authors： Helliwell SB,Wagner P,Kunz J,Deuter-Reinhard M,Henriquez R,Hall MN

更新日期：1994-01-01 00:00:00

abstract：:During vertebrate neuromuscular junction (NMJ) assembly, motor axons and their muscle targets exchange short-range signals that regulate the subsequent steps of presynaptic and postsynaptic specialization. We report here that this interaction is in part mediated by axonal filopodia extended preferentially by cultured ...

journal_title：Molecular biology of the cell

authors： Li PP,Chen C,Lee CW,Madhavan R,Peng HB

更新日期：2011-07-15 00:00:00

abstract：:Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. One feature of VEGF is the existence of multiple isoforms arising from alternative exon splicing. Our initial biochemical and biological studies indicated that such isoforms are uniquely suited to gen...

journal_title：Molecular biology of the cell

authors： Ferrara N

更新日期：2010-03-01 00:00:00

abstract：:Phosphoinositide 3-kinases (PI3K) are key molecular players in male fertility. However, the specific roles of different p110 PI3K catalytic subunits within the spermatogenic lineage have not been characterized so far. Herein, we report that male mice expressing a catalytically inactive p110beta develop testicular hypo...

journal_title：Molecular biology of the cell

authors： Ciraolo E,Morello F,Hobbs RM,Wolf F,Marone R,Iezzi M,Lu X,Mengozzi G,Altruda F,Sorba G,Guan K,Pandolfi PP,Wymann MP,Hirsch E

更新日期：2010-03-01 00:00:00

abstract：:In mammals, a finite population of oocytes is generated during embryogenesis, and proper oocyte meiotic divisions are crucial for fertility. Sperm-associated antigen 1 (SPAG-1) has been implicated in infertility and tumorigenesis; however, its relevance in cell cycle programs remains rudimentary. Here we explore a nov...

journal_title：Molecular biology of the cell

authors： Huang C,Wu D,Khan FA,Jiao X,Guan K,Huo L

更新日期：2016-06-01 00:00:00

abstract：:In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significan...

journal_title：Molecular biology of the cell

authors： Turowski P,Franckhauser C,Morris MC,Vaglio P,Fernandez A,Lamb NJ

更新日期：2003-07-01 00:00:00

abstract：:The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase Fab1/PIKfyve controls trafficking but not silencing of endocytosed receptors. Drosophila fab1 mutants contain undetecta...

journal_title：Molecular biology of the cell

authors： Rusten TE,Rodahl LM,Pattni K,Englund C,Samakovlis C,Dove S,Brech A,Stenmark H

更新日期：2006-09-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is a key regulator of mitotic progression and cell division in eukaryotes. It is highly expressed in tumor cells and considered a potential target for cancer therapy. Here, we report the discovery and application of a novel potent small-molecule inhibitor of mammalian Plk1, ZK-Thiazolidinone ...

journal_title：Molecular biology of the cell

authors： Santamaria A,Neef R,Eberspächer U,Eis K,Husemann M,Mumberg D,Prechtl S,Schulze V,Siemeister G,Wortmann L,Barr FA,Nigg EA

更新日期：2007-10-01 00:00:00

abstract：:Vascular integrity and the maintenance of blood vessel continuity are fundamental features of the circulatory system maintained through endothelial cell-cell junctions. Defects in the endothelial barrier become an initiating factor in several pathologies, including ischemia/reperfusion, tumor angiogenesis, pulmonary e...

journal_title：Molecular biology of the cell

authors： Birukova AA,Singleton PA,Gawlak G,Tian X,Mirzapoiazova T,Mambetsariev B,Dubrovskyi O,Oskolkova OV,Bochkov VN,Birukov KG

更新日期：2014-07-01 00:00:00

abstract：:The early Caenorhabditis elegans embryo contains abundant transcripts for two alpha- and two beta-tubulins, raising the question of whether each isoform performs specialized functions or simply contributes to total tubulin levels. Our identification of two recessive, complementing alleles of a beta-tubulin that disrup...

journal_title：Molecular biology of the cell

authors： Wright AJ,Hunter CP

更新日期：2003-11-01 00:00:00

abstract：:In epithelial cell lines, apical but not basolateral clathrin-mediated endocytosis has been shown to be affected by actin-disrupting drugs. Using electron and fluorescence microscopy, as well as biochemical assays, we show that the amount of actin dedicated to endocytosis is limiting at the apical surface of epithelia...

journal_title：Molecular biology of the cell

authors： Hyman T,Shmuel M,Altschuler Y

更新日期：2006-01-01 00:00:00

abstract：:Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

journal_title：Molecular biology of the cell

authors： Tapia-Alveal C,O'Connell MJ

更新日期：2011-12-01 00:00:00

abstract：:More than 130 different mutations in the gap junction integral plasma membrane protein connexin32 (Cx32) have been linked to the human peripheral neuropathy X-linked Charcot-Marie-Tooth disease (CMTX). How these various mutants are processed by the cell and the mechanism(s) by which they cause CMTX are unknown. To add...

journal_title：Molecular biology of the cell

authors： VanSlyke JK,Deschenes SM,Musil LS

更新日期：2000-06-01 00:00:00

abstract：:Myosin II-dependent contraction of the contractile ring drives equatorial furrowing during cytokinesis in animal cells. Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces. Here, we show that in the presence of...

journal_title：Molecular biology of the cell

authors： Kanada M,Nagasaki A,Uyeda TQ

更新日期：2005-08-01 00:00:00