Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo.

abstract：:Intracellular survival of Salmonella relies on the activity of proteins translocated into the host cell by type III secretion systems (T3SS). The protein kinase activity of the T3SS effector SteC is required for F-actin remodeling in host cells, although no SteC target has been identified so far. Here we show that exp...

journal_title：Molecular biology of the cell

authors： Fernandez-Piñar P,Alemán A,Sondek J,Dohlman HG,Molina M,Martín H

更新日期：2012-11-01 00:00:00

abstract：:Small heat shock proteins (sHsps) bind destabilized proteins during cell stress and disease, but their physiological functions are less clear. We evaluated the impact of Hsp27, an sHsp expressed in airway epithelial cells, on the common protein misfolding mutant that is responsible for most cystic fibrosis. F508del cy...

journal_title：Molecular biology of the cell

authors： Ahner A,Gong X,Schmidt BZ,Peters KW,Rabeh WM,Thibodeau PH,Lukacs GL,Frizzell RA

更新日期：2013-01-01 00:00:00

abstract：:Abundant evidence has shown that the GTPase dynamin is required for receptor-mediated endocytosis, but its exact role in endocytic clathrin-coated vesicle formation remains to be established. Whereas dynamin GTPase domain mutants that are defective in GTP binding and hydrolysis are potent dominant-negative inhibitors ...

journal_title：Molecular biology of the cell

authors： Damke H,Binns DD,Ueda H,Schmid SL,Baba T

更新日期：2001-09-01 00:00:00

abstract：:Chromatin and nuclear pore complexes (NPCs) undergo dramatic changes during mitosis, which in vertebrates and Aspergillus nidulans involves movement of Nup2 from NPCs to the chromatin region to fulfill unknown functions. This transition is shown to require the Cdk1 mitotic kinase and be promoted prematurely by ectopic...

journal_title：Molecular biology of the cell

authors： Markossian S,Suresh S,Osmani AH,Osmani SA

更新日期：2015-02-15 00:00:00

abstract：:The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin...

journal_title：Molecular biology of the cell

authors： Shimi T,Kittisopikul M,Tran J,Goldman AE,Adam SA,Zheng Y,Jaqaman K,Goldman RD

更新日期：2015-11-05 00:00:00

abstract：:Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

journal_title：Molecular biology of the cell

authors： Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

更新日期：2018-02-15 00:00:00

abstract：:The transition between the closed and open conformations of the Sec61 complex permits nascent protein insertion into the translocation channel. A critical event in this structural transition is the opening of the lateral translocon gate that is formed by four transmembrane (TM) spans (TM2, TM3, TM7, and TM8 in Sec61p)...

journal_title：Molecular biology of the cell

authors： Trueman SF,Mandon EC,Gilmore R

更新日期：2011-09-01 00:00:00

abstract：:Budding yeast treated with hydroxyurea (HU) activate the S phase checkpoint kinase Rad53, which prevents DNA replication forks from undergoing aberrant structural transitions and nuclease processing. Rad53 is also required to prevent premature extension of the mitotic spindle that assembles during a HU-extended S phas...

journal_title：Molecular biology of the cell

authors： Julius J,Peng J,McCulley A,Caridi C,Arnak R,See C,Nugent CI,Feng W,Bachant J

更新日期：2019-10-15 00:00:00

abstract：:Bile ducts are hepatic tubular structures that are lined by cholangiocytes, a type of liver epithelial cell. Cholangiocytes first form a single layer of cells, termed the ductal plate, surrounding the portal vein, which eventually remodels into the branching tubular network of bile ducts. The process of bile duct morp...

journal_title：Molecular biology of the cell

authors： Tanimizu N,Miyajima A,Mostov KE

更新日期：2009-05-01 00:00:00

abstract：:Extended coiled-coil proteins of the Golgin family play prominent roles in maintaining the structure and function of the Golgi complex. Here we further investigate the Golgin protein Coy1 and document its function in retrograde transport between early Golgi compartments. Cells that lack Coy1 displayed a reduced half-l...

journal_title：Molecular biology of the cell

authors： Anderson NS,Mukherjee I,Bentivoglio CM,Barlowe C

更新日期：2017-08-09 00:00:00

abstract：:Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate r...

journal_title：Molecular biology of the cell

authors： Holenstein CN,Horvath A,Schär B,Schoenenberger AD,Bollhalder M,Goedecke N,Bartalena G,Otto O,Herbig M,Guck J,Müller DA,Snedeker JG,Silvan U

更新日期：2019-03-21 00:00:00

abstract：:During constitutive secretion, proteins synthesized at the endoplasmic reticulum (ER) are transported to the Golgi complex for processing and then to the plasma membrane for incorporation or extracellular release. This study uses a unique live-cell constitutive secretion assay to establish roles for the molecular moto...

journal_title：Molecular biology of the cell

authors： Bond LM,Peden AA,Kendrick-Jones J,Sellers JR,Buss F

更新日期：2011-01-01 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:GRIM-19 was found to copurify with complex I of mitochondrial respiratory chain and subsequently was demonstrated to be involved in complex I assembly and activity. To further understand its function in complex I, we dissected its functional domains by generating a number of deletion, truncation, and point mutants. Th...

journal_title：Molecular biology of the cell

authors： Lu H,Cao X

更新日期：2008-05-01 00:00:00

abstract：:Giant axonal neuropathy (GAN) is a rare disease caused by mutations in the GAN gene, which encodes gigaxonin, an E3 ligase adapter that targets intermediate filament (IF) proteins for degradation in numerous cell types, including neurons and fibroblasts. The cellular hallmark of GAN pathology is the formation of large...

journal_title：Molecular biology of the cell

authors： Lowery J,Jain N,Kuczmarski ER,Mahammad S,Goldman A,Gelfand VI,Opal P,Goldman RD

更新日期：2016-02-15 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is facilitated by a precisely regulated burst of actin assembly. PtdIns(4,5)P2 is an important signaling lipid with conserved roles in CME and actin assembly regulation. Rhomboid family multipass transmembrane proteins regulate diverse cellular processes; however, rhomboid-mediated ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Lewellyn EB,Drubin DG

更新日期：2015-04-15 00:00:00

abstract：:The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma membrane (PM). In this study, we re...

journal_title：Molecular biology of the cell

authors： Liu J,Zuo X,Yue P,Guo W

更新日期：2007-11-01 00:00:00

abstract：:The vast complexity of platelet-derived growth factor (PDGF)-induced downstream signaling pathways is well known, but the precise roles of critical players still elude us due to our lack of specific and temporal control over their activities. Accordingly, although Src family members are some of the better characterize...

journal_title：Molecular biology of the cell

authors： Shah K,Vincent F

更新日期：2005-11-01 00:00:00

abstract：:Yeast cytochrome oxidase (COX) was previously inferred to assemble from three modules, each containing one of the three mitochondrially encoded subunits and a different subset of the eight nuclear gene products that make up this respiratory complex. Pull-down assays of pulse-labeled mitochondria enabled us to characte...

journal_title：Molecular biology of the cell

authors： Su CH,McStay GP,Tzagoloff A

更新日期：2014-04-01 00:00:00

abstract：:After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing vacuoles (SCVs) undergo a maturation process that involves sequential acquisition of Rab5 and Rab7 and displacement toward the ...

journal_title：Molecular biology of the cell

authors： Harrison RE,Brumell JH,Khandani A,Bucci C,Scott CC,Jiang X,Finlay BB,Grinstein S

更新日期：2004-07-01 00:00:00

abstract：:Zonula occludens (ZO)-1/2/3 are the members of the TJ-MAGUK family of membrane-associated guanylate kinases associated with tight junctions. To investigate the role of ZO-1 (encoded by Tjp1) in vivo, ZO-1 knockout (Tjp1(-/-)) mice were generated by gene targeting. Although heterozygous mice showed normal development a...

journal_title：Molecular biology of the cell

authors： Katsuno T,Umeda K,Matsui T,Hata M,Tamura A,Itoh M,Takeuchi K,Fujimori T,Nabeshima Y,Noda T,Tsukita S,Tsukita S

更新日期：2008-06-01 00:00:00

abstract：:Proteolytic processing of the amyloid-β precursor protein (APP) and generation of amyloid-β peptide (Aβ) are key events in Alzheimer's disease (AD) pathogenesis. Cell biological and genetic evidence has implicated the low-density lipoprotein and sorting receptor LR11/SorLA in AD through mechanisms related to APP and A...

journal_title：Molecular biology of the cell

authors： Herskowitz JH,Offe K,Deshpande A,Kahn RA,Levey AI,Lah JJ

更新日期：2012-07-01 00:00:00

abstract：:Substrates critical for transformation by pp60v-src remain unknown, as does the precise role of the src homology 2 (SH2) domain in this process. To continue exploring the role of the SH2 domain in pp60v-src-mediated transformation, site-directed mutagenesis was used to create mutant v-src alleles predicted to encode p...

journal_title：Molecular biology of the cell

authors： Verderame MF

更新日期：1997-05-01 00:00:00

abstract：:In all orders of life, cell cycle progression in proliferating cells is dependent on cell growth, and the extent of growth required for cell cycle progression is proportional to growth rate. Thus, cells growing rapidly in rich nutrients are substantially larger than slow-growing cells. In budding yeast, a conserved si...

journal_title：Molecular biology of the cell

authors： Alcaide-Gavilán M,Lucena R,Banuelos S,Kellogg DR

更新日期：2020-08-15 00:00:00

abstract：:Cystic fibrosis results from mutations in the cystic fibrosis conductance regulator protein (CFTR), a cAMP/protein kinase A (PKA) and ATP-regulated Cl(-) channel. CFTR is increasingly recognized as a component of multiprotein complexes and although several inhibitory proteins to CFTR have been identified, protein comp...

journal_title：Molecular biology of the cell

authors： Borthwick LA,McGaw J,Conner G,Taylor CJ,Gerke V,Mehta A,Robson L,Muimo R

更新日期：2007-09-01 00:00:00

abstract：:The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule cylinder. We characterized the microtubule-binding properties of limited proteolysis products an...

journal_title：Molecular biology of the cell

authors： Miranda JJ,King DS,Harrison SC

更新日期：2007-07-01 00:00:00

abstract：:The auxilin family of J-domain proteins load Hsp70 onto clathrin-coated vesicles (CCVs) to drive uncoating. In vitro, auxilin function requires its ability to bind clathrin and stimulate Hsp70 ATPase activity via its J-domain. To test these requirements in vivo, we performed a mutational analysis of Swa2p, the yeast a...

journal_title：Molecular biology of the cell

authors： Xiao J,Kim LS,Graham TR

更新日期：2006-07-01 00:00:00

abstract：:Whereas lysosome-related organelles (LRO) of specialized cells display both exocytic and endocytic features, lysosomes in nonspecialized cells can also acquire the property to fuse with the plasma membrane upon an acute rise in cytosolic calcium. Here, we characterize this unconventional secretory pathway in fibroblas...

journal_title：Molecular biology of the cell

authors： Laulagnier K,Schieber NL,Maritzen T,Haucke V,Parton RG,Gruenberg J

更新日期：2011-06-15 00:00:00

abstract：:During stem cell divisions, mitotic microtubules do more than just segregate the chromosomes. They also determine whether a cell divides virtually symmetrically or asymmetrically by establishing spindle orientation and the plane of cell division. This can be decisive for the fate of the stem cell progeny. Spindle defe...

journal_title：Molecular biology of the cell

authors： Mora-Bermúdez F,Huttner WB

更新日期：2015-12-01 00:00:00

abstract：:Protein phosphatase 1 (PP1) is a ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. When transiently expressed as fluorescent protein (FP) fusions, the three PP1 isoforms, alpha, beta/delta, and gamma1, are active phosphatases with distinct localization patterns. W...

journal_title：Molecular biology of the cell

authors： Trinkle-Mulcahy L,Andrews PD,Wickramasinghe S,Sleeman J,Prescott A,Lam YW,Lyon C,Swedlow JR,Lamond AI

更新日期：2003-01-01 00:00:00