Intracellular transport, assembly, and degradation of wild-type and disease-linked mutant gap junction proteins.

abstract：:Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or b...

journal_title：Molecular biology of the cell

authors： Camus G,Segura-Morales C,Molle D,Lopez-Vergès S,Begon-Pescia C,Cazevieille C,Schu P,Bertrand E,Berlioz-Torrent C,Basyuk E

更新日期：2007-08-01 00:00:00

abstract：:Members of the LC7/Roadblock family of light chains (LCs) have been found in both cytoplasmic and axonemal dyneins. LC7a was originally identified within Chlamydomonas outer arm dynein and associates with this motor's cargo-binding region. We describe here a novel member of this protein family, termed LC7b that is als...

journal_title：Molecular biology of the cell

authors： DiBella LM,Sakato M,Patel-King RS,Pazour GJ,King SM

更新日期：2004-10-01 00:00:00

abstract：:Epidermolysis bullosa simplex associated with late-onset muscular dystrophy (EBS-MD) is an autosomal recessive disorder resulting from mutations in the plectin gene. The majority of these mutations occur within the large exon 31 encoding the central rod domain and leave the production of a low-level rodless plectin sp...

journal_title：Molecular biology of the cell

authors： Ketema M,Secades P,Kreft M,Nahidiazar L,Janssen H,Jalink K,de Pereda JM,Sonnenberg A

更新日期：2015-07-01 00:00:00

abstract：:In autosomal dominant polycystic kidney disease (ADPKD), cysts accumulate and progressively impair renal function. Mutations in PKD1 and PKD2 genes are causally linked to ADPKD, but how these mutations drive cell behaviors that underlie ADPKD pathogenesis is unknown. Human ADPKD cysts frequently express cadherin-8 (ca...

journal_title：Molecular biology of the cell

authors： Belmonte JM,Clendenon SG,Oliveira GM,Swat MH,Greene EV,Jeyaraman S,Glazier JA,Bacallao RL

更新日期：2016-11-07 00:00:00

abstract：:Hedgehog (Hh) is a secreted morphogen involved in both short- and long-range signaling necessary for tissue patterning during development. It is unclear how this dually lipidated protein is transported over a long range in the aqueous milieu of interstitial spaces. We previously showed that the long-range signaling of...

journal_title：Molecular biology of the cell

authors： Parchure A,Vyas N,Ferguson C,Parton RG,Mayor S

更新日期：2015-12-15 00:00:00

abstract：:The Cvt pathway is a biosynthetic transport route for a distinct subset of resident yeast vacuolar hydrolases, whereas macroautophagy is a nonspecific degradative mechanism that allows cell survival during starvation. Yet, these two vacuolar trafficking pathways share a number of identical molecular components and are...

journal_title：Molecular biology of the cell

authors： Reggiori F,Wang CW,Nair U,Shintani T,Abeliovich H,Klionsky DJ

更新日期：2004-05-01 00:00:00

abstract：:Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

journal_title：Molecular biology of the cell

authors： Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

更新日期：2018-02-15 00:00:00

abstract：:Endoplasmic reticulum (ER) tubules and sheets conventionally correspond to smooth and rough ER, respectively. The ratio of ER tubules-to-sheets varies in different cell types and changes in response to cellular conditions, potentially impacting the functional output of the ER. To directly test whether ER morphology im...

journal_title：Molecular biology of the cell

authors： Mukherjee RN,Levy DL

更新日期：2019-08-15 00:00:00

abstract：:Protein kinase D2 (PKD2) belongs to the PKD family of serine/threonine kinases that is activated by phorbol esters and G protein-coupled receptors (GPCRs). Its C-terminal regulatory domain comprises two cysteine-rich domains (C1a/C1b) followed by a pleckstrin homology (PH) domain. Here, we examined the role of the reg...

journal_title：Molecular biology of the cell

authors： Auer A,von Blume J,Sturany S,von Wichert G,Van Lint J,Vandenheede J,Adler G,Seufferlein T

更新日期：2005-09-01 00:00:00

abstract：:The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule cylinder. We characterized the microtubule-binding properties of limited proteolysis products an...

journal_title：Molecular biology of the cell

authors： Miranda JJ,King DS,Harrison SC

更新日期：2007-07-01 00:00:00

abstract：:Repellents evoke growth cone turning by eliciting asymmetric, localized loss of actin cytoskeleton together with changes in substratum attachment. We have demonstrated that semaphorin-3A (Sema3A)-induced growth cone detachment and collapse require eicosanoid-mediated activation of protein kinase C epsilon (PKC epsilon...

journal_title：Molecular biology of the cell

authors： Gatlin JC,Estrada-Bernal A,Sanford SD,Pfenninger KH

更新日期：2006-12-01 00:00:00

abstract：:Survivin, a subunit of the chromosome passenger complex (CPC), binds the N-terminal tail of histone H3, which is phosphorylated on T3 by Haspin kinase, and localizes the complex to the inner centromeres. We used x-ray crystallography to determine the residues of Survivin that are important in binding phosphomodified h...

journal_title：Molecular biology of the cell

authors： Niedzialkowska E,Wang F,Porebski PJ,Minor W,Higgins JM,Stukenberg PT

更新日期：2012-04-01 00:00:00

abstract：:The early Caenorhabditis elegans embryo contains abundant transcripts for two alpha- and two beta-tubulins, raising the question of whether each isoform performs specialized functions or simply contributes to total tubulin levels. Our identification of two recessive, complementing alleles of a beta-tubulin that disrup...

journal_title：Molecular biology of the cell

authors： Wright AJ,Hunter CP

更新日期：2003-11-01 00:00:00

abstract：:We used transgenic analysis in Drosophila to compare the ability of two structurally similar alpha-tubulin isoforms to support microtubule assembly in vivo. Our data revealed that even closely related alpha-tubulin isoforms have different functional capacities. Thus, in multicellular organisms, even small changes in t...

journal_title：Molecular biology of the cell

authors： Hutchens JA,Hoyle HD,Turner FR,Raff EC

更新日期：1997-03-01 00:00:00

abstract：:Cell cycle-dependent morphogenesis of unicellular organisms depends on the spatiotemporal control of cell polarity. Rho GTPases are the major players that guide cells through structural reorganizations such as cytokinesis (Rho1 dependent) and polarity establishment (Cdc42 dependent). In budding yeast, the protein Gps1...

journal_title：Molecular biology of the cell

authors： Meitinger F,Pereira G

更新日期：2017-04-01 00:00:00

abstract：:Phosphoinositide 3-kinases (PI3K) are key molecular players in male fertility. However, the specific roles of different p110 PI3K catalytic subunits within the spermatogenic lineage have not been characterized so far. Herein, we report that male mice expressing a catalytically inactive p110beta develop testicular hypo...

journal_title：Molecular biology of the cell

authors： Ciraolo E,Morello F,Hobbs RM,Wolf F,Marone R,Iezzi M,Lu X,Mengozzi G,Altruda F,Sorba G,Guan K,Pandolfi PP,Wymann MP,Hirsch E

更新日期：2010-03-01 00:00:00

abstract：:The PDZ domain-containing protein CAL mediates lysosomal trafficking and degradation of CFTR. Here we demonstrate the involvement of a CAL-binding SNARE protein syntaxin 6 (STX6) in this process. Overexpression of STX6, which colocalizes and coimmunoprecipitates with CAL, dramatically reduces the steady-state level an...

journal_title：Molecular biology of the cell

authors： Cheng J,Cebotaru V,Cebotaru L,Guggino WB

更新日期：2010-04-01 00:00:00

abstract：:Evidence has been presented both for and against obligate retrograde movement of resident Golgi proteins through the endoplasmic reticulum (ER) during nocodazole-induced Golgi ministack formation. Here, we studied the nocodazole-induced formation of ministacks using phospholipase A(2) (PLA(2)) antagonists, which have ...

journal_title：Molecular biology of the cell

authors： Drecktrah D,Brown WJ

更新日期：1999-12-01 00:00:00

abstract：:Regulator of G protein signaling (RGS) proteins inhibit G protein signaling by activating Galpha GTPase activity, but the mechanisms that regulate RGS activity are not well understood. The mammalian R7 binding protein (R7BP) can interact with all members of the R7 family of RGS proteins, and palmitoylation of R7BP can...

journal_title：Molecular biology of the cell

authors： Porter MY,Koelle MR

更新日期：2010-01-15 00:00:00

abstract：:A primary cilium is found on most mammalian cells, where it acts as a cellular antenna for the reception of both mechanical and chemical signals. A variety of diseases are associated with defective ciliogenesis, reflecting the ubiquity of the function of cilia and the number of proteins required for their assembly. Pr...

journal_title：Molecular biology of the cell

authors： Copeland SJ,McRae A,Guarguaglini G,Trinkle-Mulcahy L,Copeland JW

更新日期：2018-07-01 00:00:00

abstract：:A cell surface proteoglycan, syndecan-1, has been shown to participate in the maintenance of the epithelial cell morphology. A point mutated activated c-Ha-ras gene under the control of the glucocorticoid inducible MMTV-LTR promoter was transfected into the mouse mammary epithelial cell line, NOG-8. The NOG-8 ras cell...

journal_title：Molecular biology of the cell

authors： Kirjavainen J,Leppä S,Hynes NE,Jalkanen M

更新日期：1993-08-01 00:00:00

abstract：:Myosin II motors embedded within the actin cortex generate contractile forces to modulate cell shape in essential behaviors, including polarization, migration, and division. In sarcomeres, myosin II-mediated sliding of antiparallel F-actin is tightly coupled to myofibril contraction. By contrast, cortical F-actin is h...

journal_title：Molecular biology of the cell

authors： Murrell M,Gardel ML

更新日期：2014-06-15 00:00:00

abstract：:Many of the actions of the neuropeptide substance P (SP) that are mediated by the neurokinin 1 receptor (NK1-R) desensitize and resensitize, which may be associated with NK1-R endocytosis and recycling. We delineated this endocytic pathway in transfected cells by confocal microscopy using cyanine 3-SP and NK1-R antibo...

journal_title：Molecular biology of the cell

authors： Grady EF,Garland AM,Gamp PD,Lovett M,Payan DG,Bunnett NW

更新日期：1995-05-01 00:00:00

abstract：:A heterozygous Caveolin-1 c.474delA mutation has been identified in a family with heritable pulmonary arterial hypertension (PAH). This frameshift mutation leads to caveolin-1 protein that contains all known functional domains but has a change only in the final 20 amino acids of the C terminus. Here we studied how thi...

journal_title：Molecular biology of the cell

authors： Marsboom G,Chen Z,Yuan Y,Zhang Y,Tiruppathi C,Loyd JE,Austin ED,Machado RF,Minshall RD,Rehman J,Malik AB

更新日期：2017-03-15 00:00:00

abstract：:Dynamics of endocytic clathrin-coated structures can be remarkably divergent across different cell types, cells within the same culture, or even distinct surfaces of the same cell. The origin of this astounding heterogeneity remains to be elucidated. Here we show that cellular processes associated with changes in effe...

journal_title：Molecular biology of the cell

authors： Willy NM,Ferguson JP,Huber SD,Heidotting SP,Aygün E,Wurm SA,Johnston-Halperin E,Poirier MG,Kural C

更新日期：2017-11-15 00:00:00

abstract：:The spatiotemporal dynamics of triglyceride (TG) storage in unilocular adipocytes are not well understood. Here we applied ex vivo technology to study trafficking and metabolism of fluorescent fatty acids in adipose tissue explants. Live imaging revealed multiple cytoplasmic nodules surrounding the large central lipid...

journal_title：Molecular biology of the cell

authors： Chu M,Sampath H,Cahana DY,Kahl CA,Somwar R,Cornea A,Roberts CT Jr,Varlamov O

更新日期：2014-12-15 00:00:00

abstract：:Candida albicans must undergo a switch from white to opaque to mate. Opaque cells then release mating type-specific pheromones that induce mating responses in opaque cells. Uniquely in C. albicans, the same pheromones induce mating-incompetent white cells to become cohesive, form an adhesive basal layer of cells on a ...

journal_title：Molecular biology of the cell

authors： Yi S,Sahni N,Daniels KJ,Pujol C,Srikantha T,Soll DR

更新日期：2008-03-01 00:00:00

abstract：:Kinesin-8 motor proteins destabilize microtubules. Their absence during cell division is associated with disorganized mitotic chromosome movements and chromosome loss. Despite recent work studying effects of kinesin-8s on microtubule dynamics, it remains unclear whether the kinesin-8 mitotic phenotypes are consequence...

journal_title：Molecular biology of the cell

authors： Gergely ZR,Crapo A,Hough LE,McIntosh JR,Betterton MD

更新日期：2016-11-07 00:00:00

abstract：:EB1 is a conserved microtubule plus end tracking protein considered to play crucial roles in microtubule organization and the interaction of microtubules with the cell cortex. Despite intense studies carried out in yeast and cultured cells, the role of EB1 in multicellular systems remains to be elucidated. Here, we de...

journal_title：Molecular biology of the cell

authors： Elliott SL,Cullen CF,Wrobel N,Kernan MJ,Ohkura H

更新日期：2005-02-01 00:00:00

abstract：:Sustained directional migration of epithelial cells is essential for regeneration of injured epithelia. Front-rear polarity of migrating cells is determined by local activation of a signaling network involving Cdc42 and other factors in response to spatial cues from the environment, the nature of which are obscure. We...

journal_title：Molecular biology of the cell

authors： Greciano PG,Moyano JV,Buschmann MM,Tang J,Lu Y,Rudnicki J,Manninen A,Matlin KS

更新日期：2012-01-01 00:00:00