Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis.

abstract：:Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

journal_title：Molecular biology of the cell

authors： Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

更新日期：2014-09-15 00:00:00

abstract：:Steroids and growth factors control neuronal development through their receptors under physiological and pathological conditions. We show that PC12 cells harbor endogenous androgen receptor (AR), whose inhibition or silencing strongly interferes with neuritogenesis stimulated by the nonaromatizable synthetic androgen ...

journal_title：Molecular biology of the cell

authors： Di Donato M,Bilancio A,D'Amato L,Claudiani P,Oliviero MA,Barone MV,Auricchio A,Appella E,Migliaccio A,Auricchio F,Castoria G

更新日期：2015-08-01 00:00:00

abstract：:Apoptotic cells are cleared by phagocytosis during development, homeostasis, and pathology. However, it is still unclear how necrotic cells are removed. We compared the phagocytic uptake by macrophages of variants of L929sA murine fibrosarcoma cells induced to die by tumor necrosis factor-induced necrosis or by Fas-me...

journal_title：Molecular biology of the cell

authors： Brouckaert G,Kalai M,Krysko DV,Saelens X,Vercammen D,Ndlovu MN,Haegeman G,D'Herde K,Vandenabeele P

更新日期：2004-03-01 00:00:00

abstract：:Terminal regions of Drosophila embryos are patterned by signaling through ERK, which is genetically deregulated in multiple human diseases. Quantitative studies of terminal patterning have been recently used to investigate gain-of-function variants of human MEK1, encoding the MEK kinase that directly activates ERK by ...

journal_title：Molecular biology of the cell

authors： Marmion RA,Yang L,Goyal Y,Jindal GA,Wetzel JL,Singh M,Schüpbach T,Shvartsman SY

更新日期：2021-01-21 00:00:00

abstract：:The tumor suppressor protein p53 mediates stress-induced growth arrest or apoptosis and plays a major role in safeguarding genome integrity. In response to DNA damage, p53 can be modified at multiple sites by phosphorylation and acetylation. We report on the characterization of p53 C-terminal phosphorylation by CHK1 a...

journal_title：Molecular biology of the cell

authors： Ou YH,Chung PH,Sun TP,Shieh SY

更新日期：2005-04-01 00:00:00

abstract：:Cohesion establishment and maintenance are carried out by proteins that modify the activity of Cohesin, an essential complex that holds sister chromatids together. Constituents of the replication fork, such as the DNA polymerase alpha-binding protein Ctf4, contribute to cohesion in ways that are poorly understood. To ...

journal_title：Molecular biology of the cell

authors： Warren CD,Eckley DM,Lee MS,Hanna JS,Hughes A,Peyser B,Jie C,Irizarry R,Spencer FA

更新日期：2004-04-01 00:00:00

abstract：:Numb has been implicated in cortical neurogenesis during nervous system development, as a result of its asymmetric partitioning and antagonizing Notch signaling. Recent studies have revealed that Numb functions in clathrin-dependent endocytosis by binding to the AP-2 complex. Numb is also expressed in postmitotic neur...

journal_title：Molecular biology of the cell

authors： Nishimura T,Yamaguchi T,Tokunaga A,Hara A,Hamaguchi T,Kato K,Iwamatsu A,Okano H,Kaibuchi K

更新日期：2006-03-01 00:00:00

abstract：:Microtubule-associated proteins (MAPs) are a functionally highly diverse class of proteins that help to adjust the shape and function of the microtubule cytoskeleton in space and time. For this purpose, MAPs structurally support microtubules, modulate their dynamic instability, or regulate the activity of associated m...

journal_title：Molecular biology of the cell

authors： Drechsler H,Xu Y,Geyer VF,Zhang Y,Diez S

更新日期：2019-11-15 00:00:00

abstract：:Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

journal_title：Molecular biology of the cell

authors： Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

更新日期：2019-05-01 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:A morphogenesis checkpoint in budding yeast delays nuclear division (and subsequent cell cycle progression) in cells that have failed to make a bud. We show that the ability of this checkpoint to delay nuclear division requires the SWE1 gene, encoding a protein kinase that inhibits the master cell cycle regulatory kin...

journal_title：Molecular biology of the cell

authors： Sia RA,Herald HA,Lew DJ

更新日期：1996-11-01 00:00:00

abstract：:Although the distribution of the cation-independent mannose 6-phosphate receptor (CI-MPR) has been well studied, its intracellular itinerary and trafficking kinetics remain uncertain. In this report, we describe the endocytic trafficking and steady-state localization of a chimeric form of the CI-MPR containing the ect...

journal_title：Molecular biology of the cell

authors： Lin SX,Mallet WG,Huang AY,Maxfield FR

更新日期：2004-02-01 00:00:00

abstract：:Eukaryotic cells actively block entry into mitosis in the presence of DNA damage or incompletely replicated DNA. This response is mediated by signal transduction cascades called cell cycle checkpoints. We show here that the human checkpoint control protein hRAD9 physically associates with two other checkpoint control ...

journal_title：Molecular biology of the cell

authors： St Onge RP,Udell CM,Casselman R,Davey S

更新日期：1999-06-01 00:00:00

abstract：:Endocytosis modulates EGFR function by compartmentalizing and attenuating or enhancing its ligand-induced signaling. Here we show that it can also control the cell surface versus intracellular distribution of empty/inactive EGFR. Our previous observation that PKA inhibitors induce EGFR internalization prompted us to t...

journal_title：Molecular biology of the cell

authors： Norambuena A,Metz C,Jung JE,Silva A,Otero C,Cancino J,Retamal C,Valenzuela JC,Soza A,González A

更新日期：2010-08-15 00:00:00

abstract：:TPX2 is a Ran-regulated spindle assembly factor that is required for kinetochore fiber formation and activation of the mitotic kinase Aurora A. TPX2 is enriched near spindle poles and is required near kinetochores, suggesting that it undergoes dynamic relocalization throughout mitosis. Using photoactivation, we measur...

journal_title：Molecular biology of the cell

authors： Ma N,Tulu US,Ferenz NP,Fagerstrom C,Wilde A,Wadsworth P

更新日期：2010-03-15 00:00:00

abstract：:Meiotic recombination differs from mitotic recombination in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change in partner choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-s...

journal_title：Molecular biology of the cell

authors： Niu H,Wan L,Baumgartner B,Schaefer D,Loidl J,Hollingsworth NM

更新日期：2005-12-01 00:00:00

abstract：:Astrocytes in neuron-free cultures typically lack processes, although they are highly process-bearing in vivo. We show that basic fibroblast growth factor (bFGF) induces cultured astrocytes to grow processes and that Ras family GTPases mediate these morphological changes. Activated alleles of rac1 and rhoA blocked and...

journal_title：Molecular biology of the cell

authors： Kalman D,Gomperts SN,Hardy S,Kitamura M,Bishop JM

更新日期：1999-05-01 00:00:00

abstract：:Nucleosome positioning is important for the structural integrity of chromosomes. During metaphase the mitotic spindle exerts physical force on pericentromeric chromatin. The cell must adjust the pericentromeric chromatin to accommodate the changing tension resulting from microtubule dynamics to maintain a stable metap...

journal_title：Molecular biology of the cell

authors： Verdaasdonk JS,Gardner R,Stephens AD,Yeh E,Bloom K

更新日期：2012-07-01 00:00:00

abstract：:The unfolded protein response (UPR) monitors and maintains protein homeostasis in the endoplasmic reticulum (ER). In budding yeast, the UPR is a transcriptional regulatory pathway that is quiescent under normal conditions. Under conditions of acute ER stress, activation of UPR targets is essential for cell viability. ...

journal_title：Molecular biology of the cell

authors： Hsu CL,Prasad R,Blackman C,Ng DT

更新日期：2012-02-01 00:00:00

abstract：:Cell emergence onto damaged or organized fibrous extracellular matrix (ECM) is a crucial precursor to collective cell migration in wound closure and cancer metastasis, respectively. However, there is a fundamental gap in our quantitative understanding of the role of local ECM size and arrangement in cell emergence-bas...

journal_title：Molecular biology of the cell

authors： Sharma P,Ng C,Jana A,Padhi A,Szymanski P,Lee JSH,Behkam B,Nain AS

更新日期：2017-09-15 00:00:00

abstract：:The kinesin superfamily of microtubule motor proteins is important in many cellular processes, including mitosis and meiosis, vesicle transport, and the establishment and maintenance of cell polarity. We have characterized two related kinesins in fission yeast, klp5+ and klp6+,, that are amino-terminal motors of the K...

journal_title：Molecular biology of the cell

authors： West RR,Malmstrom T,Troxell CL,McIntosh JR

更新日期：2001-12-01 00:00:00

abstract：:We have identified a novel Ras-interacting protein from Dictyostelium, RIP3, whose function is required for both chemotaxis and the synthesis and relay of the cyclic AMP (cAMP) chemoattractant signal. rip3 null cells are unable to aggregate and lack receptor activation of adenylyl cyclase but are able, in response to ...

journal_title：Molecular biology of the cell

authors： Lee S,Parent CA,Insall R,Firtel RA

更新日期：1999-09-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:Endocytic adaptor proteins facilitate cargo recruitment and clathrin-coated pit nucleation. The prototypical clathrin adaptor AP2 mediates cargo recruitment, maturation, and scission of the pit by binding cargo, clathrin, and accessory proteins, including the Eps-homology (EH) domain proteins Eps15 and intersectin. Ho...

journal_title：Molecular biology of the cell

authors： Teckchandani A,Mulkearns EE,Randolph TW,Toida N,Cooper JA

更新日期：2012-08-01 00:00:00

abstract：:The Rab GTPase Ypt11 is a Myo2-binding protein implicated in mother-to-bud transport of the cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during yeast division. However, its reported subcellular localization does not reflect all of these functions. Here we show that Ypt11 is normally a low-abundanc...

journal_title：Molecular biology of the cell

authors： Lewandowska A,Macfarlane J,Shaw JM

更新日期：2013-04-01 00:00:00

abstract：:The key molecular regulatory mechanisms that govern and coordinate the molecular alterations that underpin the process of human labor remain incompletely understood although enhanced intrauterine prostaglandin production is known to be requisite. Studies from cancer tissues have indicated that at least one key enzyme ...

journal_title：Molecular biology of the cell

authors： Mitchell MD

更新日期：2006-01-01 00:00:00

abstract：:Phospholipase D (PLD) has been suggested to mediate epidermal growth factor (EGF) signaling. However, the molecular mechanism of EGF-induced PLD activation has not yet been elucidated. We investigated the importance of the phosphorylation and compartmentalization of PLD1 in EGF signaling. EGF treatment of COS-7 cells ...

journal_title：Molecular biology of the cell

authors： Han JM,Kim Y,Lee JS,Lee CS,Lee BD,Ohba M,Kuroki T,Suh PG,Ryu SH

更新日期：2002-11-01 00:00:00

abstract：:Retroviral assembly is driven by Gag, and nascent viral particles escape cells by recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show that the clathrin adaptor complex AP-1 is involved in retroviral release. The absence of AP-1mu obtained by genetic knock-out or b...

journal_title：Molecular biology of the cell

authors： Camus G,Segura-Morales C,Molle D,Lopez-Vergès S,Begon-Pescia C,Cazevieille C,Schu P,Bertrand E,Berlioz-Torrent C,Basyuk E

更新日期：2007-08-01 00:00:00

abstract：:Proteins of the SNARE (soluble N-ethylmalemide-sensitive factor attachment protein receptor) family are essential for the fusion of transport vesicles with an acceptor membrane. Despite considerable sequence divergence, their mechanism of action is conserved: heterologous sets assemble into membrane-bridging SNARE com...

journal_title：Molecular biology of the cell

authors： Kloepper TH,Kienle CN,Fasshauer D

更新日期：2007-09-01 00:00:00