Abstract:
:Eukaryotic cells actively block entry into mitosis in the presence of DNA damage or incompletely replicated DNA. This response is mediated by signal transduction cascades called cell cycle checkpoints. We show here that the human checkpoint control protein hRAD9 physically associates with two other checkpoint control proteins, hRAD1 and hHUS1. Furthermore, hRAD1 and hHUS1 themselves interact, analogously to their fission yeast homologues Rad1 and Hus1. We also show that hRAD9 is present in multiple phosphorylation forms in vivo. These phosphorylated forms are present in tissue culture cells that have not been exposed to exogenous sources of DNA damage, but it remains possible that endogenous damage or naturally occurring replication intermediates cause the observed phosphorylation. Finally, we show that hRAD9 is a nuclear protein, indicating that in this signal transduction pathway, hRAD9 is physically proximal to the upstream (DNA damage) signal rather than to the downstream, cytoplasmic, cell cycle machinery.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
St Onge RP,Udell CM,Casselman R,Davey Sdoi
10.1091/mbc.10.6.1985keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
1985-95issue
6eissn
1059-1524issn
1939-4586journal_volume
10pub_type
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