Abstract:
:Cohesion establishment and maintenance are carried out by proteins that modify the activity of Cohesin, an essential complex that holds sister chromatids together. Constituents of the replication fork, such as the DNA polymerase alpha-binding protein Ctf4, contribute to cohesion in ways that are poorly understood. To identify additional cohesion components, we analyzed a ctf4Delta synthetic lethal screen performed on microarrays. We focused on a subset of ctf4Delta-interacting genes with genetic instability of their own. Our analyses revealed that 17 previously studied genes are also necessary for the maintenance of robust association of sisters in metaphase. Among these were subunits of the MRX complex, which forms a molecular structure similar to Cohesin. Further investigation indicated that the MRX complex did not contribute to metaphase cohesion independent of Cohesin, although an additional role may be contributed by XRS2. In general, results from the screen indicated a sister chromatid cohesion role for a specific subset of genes that function in DNA replication and repair. This subset is particularly enriched for genes that support the S-phase checkpoint. We suggest that these genes promote and protect a chromatin environment conducive to robust cohesion.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Warren CD,Eckley DM,Lee MS,Hanna JS,Hughes A,Peyser B,Jie C,Irizarry R,Spencer FAdoi
10.1091/mbc.e03-09-0637keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1724-35issue
4eissn
1059-1524issn
1939-4586pii
E03-09-0637journal_volume
15pub_type
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