Abstract:
:Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome-wide screen, we identify 1450 genes whose transcription is regulated by Brd2. In addition, almost 290 genes change their alternative splicing pattern upon Brd2 depletion. Brd2 is specifically localized at promoters of target genes, and our data show that Brd2 interaction with chromatin cannot be explained solely by histone acetylation. Using coimmunoprecipitation and live-cell imaging, we show that the C-terminal part is crucial for Brd2 association with chromatin. Live-cell microscopy also allows us to map the average binding time of Brd2 to chromatin and quantify the contributions of individual Brd2 domains to the interaction with chromatin. Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Hnilicová J,Hozeifi S,Stejskalová E,Dušková E,Poser I,Humpolíčková J,Hof M,Staněk Ddoi
10.1091/mbc.E13-06-0303subject
Has Abstractpub_date
2013-11-01 00:00:00pages
3557-68issue
22eissn
1059-1524issn
1939-4586pii
mbc.E13-06-0303journal_volume
24pub_type
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