Dissection of combinatorial control by the Met4 transcriptional complex.

abstract：:CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 m...

journal_title：Molecular biology of the cell

authors： Schmidt M,Varma A,Drgon T,Bowers B,Cabib E

更新日期：2003-05-01 00:00:00

abstract：:To further elucidate the functions of actin in budding yeast and to relate actin structure to specific roles and interactions in vivo, we determined the phenotypes caused by 13 charged-to-alanine mutations isolated previously in the single Saccharomyces cerevisiae actin gene. Defects in actin organization, morphogenes...

journal_title：Molecular biology of the cell

authors： Drubin DG,Jones HD,Wertman KF

更新日期：1993-12-01 00:00:00

abstract：:The formation and disruption of synaptic connections during development are a fundamental step in neural circuit formation. Subneuronal structures such as neurites are known to be sensitive to the level of spontaneous neuronal activity, but the specifics of how neurotransmitter-induced calcium activity regulates neuri...

journal_title：Molecular biology of the cell

authors： Pearce KM,Bell M,Linthicum WH,Wen Q,Srinivasan J,Rangamani P,Scarlata S

更新日期：2020-03-19 00:00:00

abstract：:Secretory proteins are exported from the endoplasmic reticulum in COPII vesicles. SNARE proteins-core machinery for membrane fusion-are incorporated into COPII vesicles by direct interaction with Sec24. Here we report a novel mechanism for sorting of the ER-Golgi Q-SNAREs into COPII vesicles. Different mammalian Sec24...

journal_title：Molecular biology of the cell

authors： Adolf F,Rhiel M,Reckmann I,Wieland FT

更新日期：2016-09-01 00:00:00

abstract：:Intracellular trafficking of tRNA was long thought to be a one-way trip from the site of biogenesis in the nucleus to the translation machinery in the cytoplasm. This view has recently been challenged, however, by the discovery that tRNA can move retrograde from the cytoplasm back to the nucleus in Saccharomyces cerev...

journal_title：Molecular biology of the cell

authors： Chafe SC,Pierce JB,Eswara MB,McGuire AT,Mangroo D

更新日期：2011-04-01 00:00:00

abstract：:Eukaryotic cells activate the unfolded-protein response (UPR) upon endoplasmic reticulum (ER) stress, where the stress is assumed to be the accumulation of unfolded proteins in the ER. Consistent with previous in vitro studies of the ER-luminal domain of the mutant UPR initiator Ire1, our study show its association wi...

journal_title：Molecular biology of the cell

authors： Promlek T,Ishiwata-Kimata Y,Shido M,Sakuramoto M,Kohno K,Kimata Y

更新日期：2011-09-01 00:00:00

abstract：:The kidney, together with bone and intestine, plays a crucial role in maintaining whole-body calcium (Ca(2+)) homoeostasis, which is primarily mediated by altering the reabsorption of Ca(2+) filtered by the glomerulus. The transient receptor potential-vanilloid-5 (TRPV5) channel protein forms a six- transmembrane Ca(2...

journal_title：Molecular biology of the cell

authors： Cai X,Srivastava S,Surindran S,Li Z,Skolnik EY

更新日期：2014-04-01 00:00:00

abstract：:We used immunofluorescence to study the distribution and targeting of RNA polymerase (pol) III subunits and pol III transcription factors in the Xenopus laevis oocyte nucleus. Antibodies against several of these proteins stained Cajal bodies and approximately 90 specific sites on the lampbrush chromosomes. Some of the...

journal_title：Molecular biology of the cell

authors： Murphy C,Wang Z,Roeder RG,Gall JG

更新日期：2002-10-01 00:00:00

abstract：:Membrane tubules of uniform diameter (60-80 nm) and variable lengths have been seen to extend from the main bodies of the Golgi complex, trans Golgi network (TGN), and endosomes. In the case of endosomes, these tubules appear to mediate membrane and receptor recycling events. Brefeldin A (BFA) is a potent drug that co...

journal_title：Molecular biology of the cell

authors： de Figueiredo P,Brown WJ

更新日期：1995-07-01 00:00:00

abstract：:Recent evidence has shown that members of the Jak kinase family are activated after IL-6 binds to its receptor complex, leading to a tyrosine phosphorylation of gp130, the IL-6 signal-transducing subunit. The different members of the IL-6 cytokine subfamily induce distinct patterns of Jak-Tyk phosphorylation in differ...

journal_title：Molecular biology of the cell

authors： Wang Y,Fuller GM

更新日期：1994-07-01 00:00:00

abstract：:Proteins of the SNARE (soluble N-ethylmalemide-sensitive factor attachment protein receptor) family are essential for the fusion of transport vesicles with an acceptor membrane. Despite considerable sequence divergence, their mechanism of action is conserved: heterologous sets assemble into membrane-bridging SNARE com...

journal_title：Molecular biology of the cell

authors： Kloepper TH,Kienle CN,Fasshauer D

更新日期：2007-09-01 00:00:00

abstract：:Stress fibers were isolated from cultured human foreskin fibroblasts and bovine endothelial cells, and their contraction was demonstrated in vitro. Cells in culture dishes were first treated with a low-ionic-strength extraction solution and then further extracted using detergents. With gentle washes by pipetting, the ...

journal_title：Molecular biology of the cell

authors： Katoh K,Kano Y,Masuda M,Onishi H,Fujiwara K

更新日期：1998-07-01 00:00:00

abstract：:Molecular chaperones play key roles during growth, development, and stress survival. The ability to induce chaperone expression enables cells to cope with the accumulation of nonnative proteins under stress and complete developmental processes with an increased requirement for chaperone assistance. Here we generate an...

journal_title：Molecular biology of the cell

authors： Rogon C,Ulbricht A,Hesse M,Alberti S,Vijayaraj P,Best D,Adams IR,Magin TM,Fleischmann BK,Höhfeld J

更新日期：2014-08-01 00:00:00

abstract：:Although all microtubules within a single cell are polymerized from virtually identical subunits, different microtubule populations carry out specialized and diverse functions, including directional transport, force generation, and cellular morphogenesis. Functional differentiation requires specific targeting of assoc...

journal_title：Molecular biology of the cell

authors： Liu J,He Y,Benmerzouga I,Sullivan WJ Jr,Morrissette NS,Murray JM,Hu K

更新日期：2016-02-01 00:00:00

abstract：:SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosph...

journal_title：Molecular biology of the cell

authors： Sakurai C,Itakura M,Kinoshita D,Arai S,Hashimoto H,Wada I,Hatsuzawa K

更新日期：2018-07-15 00:00:00

abstract：:cAR1, a G protein-coupled receptor (GPCR) for cAMP, is required for the multicellular development of Dictyostelium. The activation of multiple pathways by cAR1 is transient because of poorly defined adaptation mechanisms. To investigate this, we used a genetic screen for impaired development to isolate four dominant-n...

journal_title：Molecular biology of the cell

authors： Zhang M,Goswami M,Hereld D

更新日期：2005-02-01 00:00:00

abstract：:Arg8-vasopressin (AVP) promotes the differentiation of myogenic cell lines and mouse primary satellite cells by mechanisms involving the transcriptional activation of myogenic bHLH regulatory factors and myocyte enhancer factor 2 (MEF2). We here report that AVP treatment of L6 cells results in the activation of calcin...

journal_title：Molecular biology of the cell

authors： Scicchitano BM,Spath L,Musarò A,Molinaro M,Rosenthal N,Nervi C,Adamo S

更新日期：2005-08-01 00:00:00

abstract：:Bloom syndrome (BS) is a rare autosomal recessive disorder characterized by growth deficiency, immunodeficiency, genomic instability, and the early development of cancers of many types. BLM, the protein encoded by BLM, the gene mutated in BS, is localized in nuclear foci and absent from BS cells. BLM encodes a DNA hel...

journal_title：Molecular biology of the cell

authors： Neff NF,Ellis NA,Ye TZ,Noonan J,Huang K,Sanz M,Proytcheva M

更新日期：1999-03-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Mechanosensing describes the ability of a cell to sense mechanical cues of its microenvironment, including not only all components of force, stress, and strain but also substrate rigidity, topology, and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the ch...

journal_title：Molecular biology of the cell

authors： Chen Y,Ju L,Rushdi M,Ge C,Zhu C

更新日期：2017-11-07 00:00:00

abstract：:Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

journal_title：Molecular biology of the cell

authors： Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

更新日期：2014-09-15 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00

abstract：:Signals conveyed through the RAS-ERK pathway are essential for the determination of cell fate. It is well established that signal variability is achieved in the different microenvironments in which signals unfold. It is also known that signal duration is critical for decisions concerning cell commitment. However, it i...

journal_title：Molecular biology of the cell

authors： Herrero A,Casar B,Colón-Bolea P,Agudo-Ibáñez L,Crespo P

更新日期：2016-06-15 00:00:00

abstract：:A major goal of synthetic biology is to define the minimal cellular machinery required to assemble a biological structure in its simplest form. Here, we focused on Saccharomyces cerevisiae actin cables, which provide polarized tracks for intracellular transport and maintain defined lengths while continuously undergoin...

journal_title：Molecular biology of the cell

authors： Pollard LW,Garabedian MV,Alioto SL,Shekhar S,Goode BL

更新日期：2020-03-01 00:00:00

abstract：:The Notch signaling pathway plays essential roles in both animal development and human disease. Regulation of Notch receptor levels in membrane compartments has been shown to affect signaling in a variety of contexts. Here we used steady-state and pulse-labeling techniques to follow Notch receptors in sensory organ pr...

journal_title：Molecular biology of the cell

authors： Johnson SA,Zitserman D,Roegiers F

更新日期：2016-09-15 00:00:00

abstract：:The small GTPase Ran is essential for spindle assembly. Ran is proposed to act through its nuclear import receptors importin alpha and/or importin beta to control the sequestration of proteins necessary for spindle assembly. To date, the molecular mechanisms by which the Ran pathway functions remain unclear. Using pur...

journal_title：Molecular biology of the cell

authors： Ems-McClung SC,Zheng Y,Walczak CE

更新日期：2004-01-01 00:00:00

abstract：:The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein heavy chain and may mediate the attachment of dynein to centrosomes and o...

journal_title：Molecular biology of the cell

authors： Mische S,He Y,Ma L,Li M,Serr M,Hays TS

更新日期：2008-11-01 00:00:00

abstract：:SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets the nuclear envelope of C. psittaci-infected cells and uninfected neighboring cells. Digitonin-permeabilization studies of SINC-GFP-transfected HeLa cells indicate that SINC targets the inner nuclear membrane. SI...

journal_title：Molecular biology of the cell

authors： Mojica SA,Hovis KM,Frieman MB,Tran B,Hsia RC,Ravel J,Jenkins-Houk C,Wilson KL,Bavoil PM

更新日期：2015-05-15 00:00:00

abstract：:The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail ...

journal_title：Molecular biology of the cell

authors： Pollard TD

更新日期：2010-12-01 00:00:00

abstract：:In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD). Although previous analyzes have shown that conditions that impede translation termination at nonsense codons also pre...

journal_title：Molecular biology of the cell

authors： Buzina A,Shulman MJ

更新日期：1999-03-01 00:00:00