HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis.

abstract：:The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule lo...

journal_title：Molecular biology of the cell

authors： Hermann GJ,Schroeder LK,Hieb CA,Kershner AM,Rabbitts BM,Fonarev P,Grant BD,Priess JR

更新日期：2005-07-01 00:00:00

abstract：:The ubiquitin-like hPLIC proteins can associate with proteasomes, and hPLIC overexpression can specifically interfere with ubiquitin-mediated proteolysis (Kleijnen et al., 2000). Because the hPLIC proteins can also interact with certain E3 ubiquitin protein ligases, they may provide a link between the ubiquitination a...

journal_title：Molecular biology of the cell

authors： Kleijnen MF,Alarcon RM,Howley PM

更新日期：2003-09-01 00:00:00

abstract：:Cell shape is regulated by cell adhesion and cytoskeletal and membrane dynamics. Cell shape, adhesion, and motility have a complex relationship and understanding them is important in understanding developmental patterning and embryogenesis. Here we show that the lipid kinase phosphatidylinositol 4-kinase III beta (PI4...

journal_title：Molecular biology of the cell

authors： Bilodeau P,Jacobsen D,Law-Vinh D,Lee JM

更新日期：2020-08-01 00:00:00

abstract：:Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcrip...

journal_title：Molecular biology of the cell

authors： González-Aguilera C,Tous C,Babiano R,de la Cruz J,Luna R,Aguilera A

更新日期：2011-08-01 00:00:00

abstract：:DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we express...

journal_title：Molecular biology of the cell

authors： Lee HO,Zacharek SJ,Xiong Y,Duronio RJ

更新日期：2010-11-01 00:00:00

abstract：:Caveolae are vesicular invaginations of the plasma membrane. Caveolin-3 is the principal structural component of caveolae in skeletal muscle cells in vivo. We have recently generated caveolin-3 transgenic mice and demonstrated that overexpression of wild-type caveolin-3 in skeletal muscle fibers is sufficient to induc...

journal_title：Molecular biology of the cell

authors： Volonte D,Peoples AJ,Galbiati F

更新日期：2003-10-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To...

journal_title：Molecular biology of the cell

authors： Patel-King RS,King SM

更新日期：2016-04-15 00:00:00

abstract：:Myo4p, a single-headed and nonprocessive class V myosin in budding yeast, transports >20 different mRNAs asymmetrically to the bud. Here, we determine the features of the Myo4p motor that are necessary for correct localization of ASH1 mRNA to the daughter cell, a process that also requires the adapter protein She3p an...

journal_title：Molecular biology of the cell

authors： Bookwalter CS,Lord M,Trybus KM

更新日期：2009-07-01 00:00:00

abstract：:An in vitro transport assay, established with a modified Shiga toxin B subunit (STxB) as a marker, has proved to be useful for the study of transport from the early/recycling endosome (EE/RE) to the trans-Golgi network (TGN). Here, we modified this assay to test antibodies to all known soluble N-ethylmaleimide-sensiti...

journal_title：Molecular biology of the cell

authors： Tai G,Lu L,Wang TL,Tang BL,Goud B,Johannes L,Hong W

更新日期：2004-09-01 00:00:00

abstract：:The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein,...

journal_title：Molecular biology of the cell

authors： Nagy G,Milosevic I,Mohrmann R,Wiederhold K,Walter AM,Sørensen JB

更新日期：2008-09-01 00:00:00

abstract：:Proper chromosome segregation is essential for faithful cell division and if not maintained results in defective cell function caused by the abnormal distribution of genetic information. Polo-like kinase 1-interacting checkpoint helicase (PICH) is a DNA translocase essential for chromosome bridge resolution during mit...

journal_title：Molecular biology of the cell

authors： Hassebroek VA,Park H,Pandey N,Lerbakken BT,Aksenova V,Arnaoutov A,Dasso M,Azuma Y

更新日期：2020-11-01 00:00:00

abstract：:The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1. Hof1 colocalizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to the actomyosin ring (AMR). Neither the function of Hof1 a...

journal_title：Molecular biology of the cell

authors： Meitinger F,Palani S,Hub B,Pereira G

更新日期：2013-05-01 00:00:00

abstract：:Growth factor stimulation induces the formation of dynamic actin structures known as dorsal ruffles. Mammalian actin-binding protein-1 (mAbp1) is an actin-binding protein that has been implicated in regulating clathrin-mediated endocytosis; however, a role for mAbp1 in regulating the dynamics of growth factor-induced ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Perrin BJ,Bennin DA,Huttenlocher A

更新日期：2010-01-01 00:00:00

abstract：:Activation of the high-osmolarity glycerol (HOG) pathway for osmoregulation in the yeast Saccharomyces cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p. We have determined the solution structure of the Ste50p-RA (Ras association) domain, and it shows...

journal_title：Molecular biology of the cell

authors： Ekiel I,Sulea T,Jansen G,Kowalik M,Minailiuc O,Cheng J,Harcus D,Cygler M,Whiteway M,Wu C

更新日期：2009-12-01 00:00:00

abstract：:Endoplasmic reticulum (ER) calcium homeostasis is disrupted in diverse pathologies, including neurodegeneration, cardiovascular diseases, and diabetes. Temporally defining calcium dysregulation during disease progression, however, has been challenging. Here we describe secreted ER calcium-monitoring proteins (SERCaMPs...

journal_title：Molecular biology of the cell

authors： Henderson MJ,Wires ES,Trychta KA,Richie CT,Harvey BK

更新日期：2014-09-15 00:00:00

abstract：:Endocytic adaptor proteins facilitate cargo recruitment and clathrin-coated pit nucleation. The prototypical clathrin adaptor AP2 mediates cargo recruitment, maturation, and scission of the pit by binding cargo, clathrin, and accessory proteins, including the Eps-homology (EH) domain proteins Eps15 and intersectin. Ho...

journal_title：Molecular biology of the cell

authors： Teckchandani A,Mulkearns EE,Randolph TW,Toida N,Cooper JA

更新日期：2012-08-01 00:00:00

abstract：:Wounding of endothelial cells is associated with altered direct intercellular communication. To determine whether gap junctional communication participates to the wound repair process, we have compared connexin (Cx) expression, cell-to-cell coupling and kinetics of wound repair in monolayer cultures of PymT-transforme...

journal_title：Molecular biology of the cell

authors： Kwak BR,Pepper MS,Gros DB,Meda P

更新日期：2001-04-01 00:00:00

abstract：:Nuclear Dbf2-related (NDR) protein kinases are essential components of regulatory pathways involved in cell morphogenesis, cell cycle control, and viability in eukaryotic cells. For their activity and function, these kinases require interaction with Mob proteins. However, little is known about how the Mob proteins are...

journal_title：Molecular biology of the cell

authors： Gutiérrez-Escribano P,González-Novo A,Suárez MB,Li CR,Wang Y,de Aldana CR,Correa-Bordes J

更新日期：2011-07-15 00:00:00

abstract：:Subcellular targeting and the activity of facilitative glucose transporters are likely to be regulated by interactions with cellular proteins. This report describes the identification and characterization of a protein, GLUT1 C-terminal binding protein (GLUT1CBP), that binds via a PDZ domain to the C terminus of GLUT1....

journal_title：Molecular biology of the cell

authors： Bunn RC,Jensen MA,Reed BC

更新日期：1999-04-01 00:00:00

abstract：:Discoidin I expression was used as a marker to screen for mutants affected in the growth-differentiation transition (GDT) of Dictyostelium. By REMI mutagenesis we have isolated mutant 2-9, an overexpressor of discoidin I. It displays normal morphogenesis but shows premature entry into the developmental cycle. The disr...

journal_title：Molecular biology of the cell

authors： Zeng C,Anjard C,Riemann K,Konzok A,Nellen W

更新日期：2000-05-01 00:00:00

abstract：:Mutations in the cytolinker protein plectin lead to grossly distorted morphology of neuromuscular junctions (NMJs) in patients suffering from epidermolysis bullosa simplex (EBS)-muscular dystrophy (MS) with myasthenic syndrome (MyS). Here we investigated whether plectin contributes to the structural integrity of NMJs ...

journal_title：Molecular biology of the cell

authors： Mihailovska E,Raith M,Valencia RG,Fischer I,Al Banchaabouchi M,Herbst R,Wiche G

更新日期：2014-12-15 00:00:00

abstract：:Activation of hepatic stellate cells during liver fibrosis is a major event facilitating an increase in extracellular matrix deposition. The up-regulation of smooth muscle alpha-actin and collagen type I is indicative of the activation process. The involvement of cysteine cathepsins, a class of lysosomal cysteine prot...

journal_title：Molecular biology of the cell

authors： Maubach G,Lim MC,Zhuo L

更新日期：2008-10-01 00:00:00

abstract：:In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...

journal_title：Molecular biology of the cell

authors： Deaton JD,Guerrero T,Howard TH

更新日期：1992-12-01 00:00:00

abstract：:Nuclear factor kappa B (NF-kappa B) modulates the expression of numerous genes via interaction with a specific DNA sequence termed the kappa B site. Its activity is modulated by a cytosolic inhibitor protein termed I kappa B, and its activation occurs in response to a variety of agents in a variety of cell types, most...

journal_title：Molecular biology of the cell

authors： Olashaw NE,Kowalik TF,Huang ES,Pledger WJ

更新日期：1992-10-01 00:00:00

abstract：:Synaptosomal associated protein of 23 kDa (SNAP-23), a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), has been implicated in phagocytosis by macrophages. For elucidation of its precise role in this process, a macrophage line overexpressing monomeric Venus-tagge...

journal_title：Molecular biology of the cell

authors： Sakurai C,Hashimoto H,Nakanishi H,Arai S,Wada Y,Sun-Wada GH,Wada I,Hatsuzawa K

更新日期：2012-12-01 00:00:00

abstract：:Proper functioning of the protein-folding quality control network depends on the network's ability to discern diverse structural perturbations to the native states of its protein substrates. Despite the centrality of the detection of misfolded states to cell home-ostasis, very little is known about the exact sequence ...

journal_title：Molecular biology of the cell

authors： Furth N,Gertman O,Shiber A,Alfassy OS,Cohen I,Rosenberg MM,Doron NK,Friedler A,Ravid T

更新日期：2011-12-01 00:00:00

abstract：:Repellents evoke growth cone turning by eliciting asymmetric, localized loss of actin cytoskeleton together with changes in substratum attachment. We have demonstrated that semaphorin-3A (Sema3A)-induced growth cone detachment and collapse require eicosanoid-mediated activation of protein kinase C epsilon (PKC epsilon...

journal_title：Molecular biology of the cell

authors： Gatlin JC,Estrada-Bernal A,Sanford SD,Pfenninger KH

更新日期：2006-12-01 00:00:00

abstract：:Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and is an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, a representative output of growth facto...

journal_title：Molecular biology of the cell

authors： Shen H,Ferguson SM,Dephoure N,Park R,Yang Y,Volpicelli-Daley L,Gygi S,Schlessinger J,De Camilli P

更新日期：2011-02-15 00:00:00

abstract：:Scientific societies aiming to foster inclusion of scientists from underrepresented (UR) backgrounds among their membership often delegate primary responsibility for this goal to a diversity-focused committee. The National Science Foundation has funded the creation of the Alliance to Catalyze Change for Equity in STEM...

journal_title：Molecular biology of the cell

authors： Segarra VA,Primus C,Unguez GA,Edwards A,Etson C,Flores SC,Fry C,Guillory AN,Ingram SL,Lawson M,McGee R,Paxson S,Phelan L,Suggs K,Vega LR,Vuong E,Havran JC,Leon A,Burton MD,Lujan JL,Ramirez-Alvarado M

更新日期：2020-11-01 00:00:00