Abstract:
:The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized, lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Hermann GJ,Schroeder LK,Hieb CA,Kershner AM,Rabbitts BM,Fonarev P,Grant BD,Priess JRdoi
10.1091/mbc.e05-01-0060keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
3273-88issue
7eissn
1059-1524issn
1939-4586pii
E05-01-0060journal_volume
16pub_type
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