Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit.

abstract：:Desmogleins (Dsgs) are cadherin family adhesion molecules essential for epidermal integrity. Previous studies have shown that desmogleins associate with lipid rafts, but the significance of this association was not clear. Here, we report that the desmoglein transmembrane domain (TMD) is the primary determinant of raft...

journal_title：Molecular biology of the cell

authors： Lewis JD,Caldara AL,Zimmer SE,Stahley SN,Seybold A,Strong NL,Frangakis AS,Levental I,Wahl JK 3rd,Mattheyses AL,Sasaki T,Nakabayashi K,Hata K,Matsubara Y,Ishida-Yamamoto A,Amagai M,Kubo A,Kowalczyk AP

更新日期：2019-06-01 00:00:00

abstract：:The amino acid-derived polyamines are organic cations that are essential for growth in all mammalian cells, but their exact roles at the molecular level remain largely unknown. Here we provide evidence that polyamines promote the translation of cyclin-dependent kinase 4 (CDK4) by the action of CUG-binding protein 1 (C...

journal_title：Molecular biology of the cell

authors： Xiao L,Cui YH,Rao JN,Zou T,Liu L,Smith A,Turner DJ,Gorospe M,Wang JY

更新日期：2011-09-01 00:00:00

abstract：:Trends in increased tuberculosis infection and a fatality rate of approximately 23% have necessitated the search for alternative biomarkers using newly developed postgenomic approaches. Here we provide a systematic analysis of Mycobacterium tuberculosis (Mtb) by directly profiling its gene products. This analysis comb...

journal_title：Molecular biology of the cell

authors： Mawuenyega KG,Forst CV,Dobos KM,Belisle JT,Chen J,Bradbury EM,Bradbury AR,Chen X

更新日期：2005-01-01 00:00:00

abstract：:Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)-anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin Va (MyoVa)-dependent secretory granule (SG) transport and secretion in...

journal_title：Molecular biology of the cell

authors： Brozzi F,Lajus S,Diraison F,Rajatileka S,Hayward K,Regazzi R,Molnár E,Váradi A

更新日期：2012-11-01 00:00:00

abstract：:Of the three structural maintenance of chromosomes (SMC) complexes, Smc5/6 remains the most poorly understood. Genetic studies have shown that Smc5/6 mutants are defective in homologous recombination (HR), and consistent with this, Smc5/6 is enriched at lesions. However, Smc5/6 is essential for viability, but HR is no...

journal_title：Molecular biology of the cell

authors： Tapia-Alveal C,O'Connell MJ

更新日期：2011-12-01 00:00:00

abstract：:The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endo...

journal_title：Molecular biology of the cell

authors： Tabata K,Matsunaga K,Sakane A,Sasaki T,Noda T,Yoshimori T

更新日期：2010-12-01 00:00:00

abstract：:SPOP, speckle-type POZ protein is a substrate adaptor protein of the Cullin-3/RING ubiquitin E3 complex. The spop gene is the most commonly point mutated in human primary prostate cancers, but the pathological contribution of the SPOP mutations remains unclear. In this study, we investigated several known factors that...

journal_title：Molecular biology of the cell

authors： Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

更新日期：2020-03-15 00:00:00

abstract：:The role of platelet endothelial cell adhesion molecule-1 (PECAM-1) in endothelial cell-cell interactions and its contribution to cadherin-mediated cell adhesion are poorly understood. Such studies have been difficult because all known endothelial cells express PECAM-1. We have used Madin-Darby canine kidney (MDCK) ce...

journal_title：Molecular biology of the cell

authors： Sheibani N,Sorenson CM,Frazier WA

更新日期：2000-08-01 00:00:00

abstract：:The serotonin (5-hydroxytryptamine; 5-HT)(2C) receptor is a G protein-coupled receptor (GPCR) exclusively expressed in CNS that has been implicated in numerous brain disorders, including anxio-depressive states. Like many GPCRs, 5-HT(2C) receptors physically interact with a variety of intracellular proteins in additio...

journal_title：Molecular biology of the cell

authors： Labasque M,Reiter E,Becamel C,Bockaert J,Marin P

更新日期：2008-11-01 00:00:00

abstract：:The survival motor neuron (SMN) complex fulfils essential functions in the assembly of snRNPs, which are key components in the splicing of pre-mRNAs. Little is known about the regulation of SMN complex activity by posttranslational modification despite its complicated phosphorylation pattern. Several phosphatases had ...

journal_title：Molecular biology of the cell

authors： Husedzinovic A,Neumann B,Reymann J,Draeger-Meurer S,Chari A,Erfle H,Fischer U,Gruss OJ

更新日期：2015-01-15 00:00:00

abstract：:Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Cae...

journal_title：Molecular biology of the cell

authors： Bar DZ,Davidovich M,Lamm AT,Zer H,Wilson KL,Gruenbaum Y

更新日期：2014-04-01 00:00:00

abstract：:Evidence has been presented both for and against obligate retrograde movement of resident Golgi proteins through the endoplasmic reticulum (ER) during nocodazole-induced Golgi ministack formation. Here, we studied the nocodazole-induced formation of ministacks using phospholipase A(2) (PLA(2)) antagonists, which have ...

journal_title：Molecular biology of the cell

authors： Drecktrah D,Brown WJ

更新日期：1999-12-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR) resides in the nucleo...

journal_title：Molecular biology of the cell

authors： Jády BE,Richard P,Bertrand E,Kiss T

更新日期：2006-02-01 00:00:00

abstract：:The hypoxia-inducible factor (HIF) is a master regulator of the cellular response to hypoxia. Its levels and activity are controlled by dioxygenases called prolyl-hydroxylases and factor inhibiting HIF (FIH). To activate genes, HIF has to access sequences in DNA that are integrated in chromatin. It is known that the c...

journal_title：Molecular biology of the cell

authors： Melvin A,Mudie S,Rocha S

更新日期：2011-11-01 00:00:00

abstract：:Doing scientific research can be a challenging, but creative and rewarding career. However, the challenge of doing research is orders of magnitude more difficult for scientists lacking access to the necessary resources. Resource-scarce environments are quite common worldwide and are highly contingent on the financial ...

journal_title：Molecular biology of the cell

authors： Karasmanis EP

更新日期：2018-06-01 00:00:00

abstract：:The perinucleolar compartment (PNC) is a nuclear substructure present in transformed cells. The PNC is defined by high concentrations of certain RNA binding proteins and a subset of small RNAs transcribed by RNA polymerase III (pol III), including the signal recognition particle RNA and an Alu RNA as reported here. To...

journal_title：Molecular biology of the cell

authors： Wang C,Politz JC,Pederson T,Huang S

更新日期：2003-06-01 00:00:00

abstract：:Giant axonal neuropathy (GAN) is a rare disease caused by mutations in the GAN gene, which encodes gigaxonin, an E3 ligase adapter that targets intermediate filament (IF) proteins for degradation in numerous cell types, including neurons and fibroblasts. The cellular hallmark of GAN pathology is the formation of large...

journal_title：Molecular biology of the cell

authors： Lowery J,Jain N,Kuczmarski ER,Mahammad S,Goldman A,Gelfand VI,Opal P,Goldman RD

更新日期：2016-02-15 00:00:00

abstract：:Ghrelin is an acylated peptidyl gastric hormone acting on the pituitary and hypothalamus to stimulate appetite, adiposity, and growth hormone release, through activation of growth hormone secretagogue receptor (GHSR)-1a receptor. Moreover, ghrelin features several activities such as inhibition of apoptosis, regulation...

journal_title：Molecular biology of the cell

authors： Filigheddu N,Gnocchi VF,Coscia M,Cappelli M,Porporato PE,Taulli R,Traini S,Baldanzi G,Chianale F,Cutrupi S,Arnoletti E,Ghè C,Fubini A,Surico N,Sinigaglia F,Ponzetto C,Muccioli G,Crepaldi T,Graziani A

更新日期：2007-03-01 00:00:00

abstract：:Pif1 family helicases, which are found in nearly all eukaryotes, have important roles in both nuclear and mitochondrial genome maintenance. Recently, the increasing availability of genome sequences has revealed that Pif1 helicases are also widely found in diverse prokaryotes, but it is currently unknown what physiolog...

journal_title：Molecular biology of the cell

authors： Bochman ML,Judge CP,Zakian VA

更新日期：2011-06-15 00:00:00

abstract：:Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1(+), encodes a protein with three LIM domains that is similar to paxil...

journal_title：Molecular biology of the cell

authors： Pinar M,Coll PM,Rincón SA,Pérez P

更新日期：2008-04-01 00:00:00

abstract：:We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and the determination of the role of Cdc7/Dbf4 phosphorylation of MCM2 in the initiation of DNA replication. Using immunoblotting, immunofluorescence, and high-speed automated cell-imaging analyses with antibodies specific against MCM2 and C...

journal_title：Molecular biology of the cell

authors： Tsuji T,Ficarro SB,Jiang W

更新日期：2006-10-01 00:00:00

abstract：:Exposure of neutrophils to chemoattractant induces cell polarization and migration. These behaviors require the asymmetric activation of distinct signaling pathways and cytoskeletal elements in the protruding pseudopod at the front of cells and the retracting uropod at the rear. An important outstanding question is, h...

journal_title：Molecular biology of the cell

authors： Bodin S,Welch MD

更新日期：2005-12-01 00:00:00

abstract：:Yarrowia lipolytica Pex23p and Saccharomyces cerevisiae Pex30p, Pex31p, and Pex32p comprise a family of dysferlin domain-containing peroxins. We show that the deletion of their Pichia pastoris homologues, PEX30 and PEX31, does not affect the function or division of methanol-induced peroxisomes but results in fewer and...

journal_title：Molecular biology of the cell

authors： Yan M,Rachubinski DA,Joshi S,Rachubinski RA,Subramani S

更新日期：2008-03-01 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1) protects cells from oxidative stress via the base excision repair pathway and as a redox transcriptional coactivator. It is required for tumor progression/metastasis, and its up-regulation is associated with cancer resistance. Loss of APE1 expression causes ce...

journal_title：Molecular biology of the cell

authors： Vascotto C,Bisetto E,Li M,Zeef LA,D'Ambrosio C,Domenis R,Comelli M,Delneri D,Scaloni A,Altieri F,Mavelli I,Quadrifoglio F,Kelley MR,Tell G

更新日期：2011-10-01 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00

abstract：:Stimulation of the MAPK pathway results in mitogen- and stress-activated protein kinase 1/2 (MSK1/2)-catalyzed phosphorylation of histone H3 at serine 10 or 28 and expression of immediate-early (IE) genes. In 10T1/2 mouse fibroblasts, phosphorylation of H3S10 and H3S28 occurs on different H3 molecules and in different...

journal_title：Molecular biology of the cell

authors： Khan DH,Healy S,He S,Lichtensztejn D,Klewes L,Sharma KL,Lau V,Mai S,Delcuve GP,Davie JR

更新日期：2017-03-15 00:00:00

abstract：:Androgen receptor (AR) belongs to the nuclear receptor superfamily and mediates the biological actions of male sex steroids. In this work, we have characterized a novel 130-kDa Ser/Thr protein kinase ANPK that interacts with the zinc finger region of AR in vivo and in vitro. The catalytic kinase domain of ANPK shares ...

journal_title：Molecular biology of the cell

authors： Moilanen AM,Karvonen U,Poukka H,Jänne OA,Palvimo JJ

更新日期：1998-09-01 00:00:00

abstract：:Collective cell migration is emerging as a major contributor to normal development and disease. Collective movement of border cells in the Drosophila ovary requires cooperation between two distinct cell types: four to six migratory cells surrounding two immotile cells called polar cells. Polar cells secrete a cytokine...

journal_title：Molecular biology of the cell

authors： Xiang W,Zhang D,Montell DJ

更新日期：2016-01-01 00:00:00

abstract：:Yeast TGN resident proteins that frequently cycle between the TGN and endosomes are much more slowly transported to the prevacuolar/late endosomal compartment (PVC) than other proteins. However, TGN protein transport to the PVC is accelerated in mutants lacking function of Inp53p. Inp53p contains a SacI polyphosphoino...

journal_title：Molecular biology of the cell

authors： Ha SA,Torabinejad J,DeWald DB,Wenk MR,Lucast L,De Camilli P,Newitt RA,Aebersold R,Nothwehr SF

更新日期：2003-04-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF, VEGF-A) is a major regulator of physiological and pathological angiogenesis. One feature of VEGF is the existence of multiple isoforms arising from alternative exon splicing. Our initial biochemical and biological studies indicated that such isoforms are uniquely suited to gen...

journal_title：Molecular biology of the cell

authors： Ferrara N

更新日期：2010-03-01 00:00:00