Septins, under Cla4p regulation, and the chitin ring are required for neck integrity in budding yeast.

Abstract:

:CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 mutants exhibited similar morphological and septin localization defects, including aberrant and ectopic septa. Myo1p, which requires septins for localization, formed abnormally wide rings in cla4 mutants. In cultures started with unbudded cells, an inhibitor of Chs3p activity, nikkomycin Z, aggravated the abnormalities of cla4 and cdc11 mutants and gave rise to enlarged necks at the mother-bud junction, leading to cell death. It is concluded that Cla4p is required for the correct localization and/or assembly of the septin ring and that both the septin ring and the Chs3p-requiring chitin ring at the mother-bud neck cooperate in maintaining the neck constricted throughout the cell cycle, a vital function in budding yeast.

journal_name

Mol Biol Cell

authors

Schmidt M,Varma A,Drgon T,Bowers B,Cabib E

doi

10.1091/mbc.e02-08-0547

keywords:

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

2128-41

issue

5

eissn

1059-1524

issn

1939-4586

pii

E02-08-0547

journal_volume

14

pub_type

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