Regulation of the epithelial Ca²⁺ channel TRPV5 by reversible histidine phosphorylation mediated by NDPK-B and PHPT1.

abstract：:We examined the relative function of the two classes of guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factors that regulate recruitment of coat proteins on the Golgi complex. Complementary overexpression and RNA-based knockdown approaches established that GBF1 regulates COPI recruitment on cis-Golgi ...

journal_title：Molecular biology of the cell

authors： Manolea F,Claude A,Chun J,Rosas J,Melançon P

更新日期：2008-02-01 00:00:00

abstract：:Satellite cells (SCs), the resident adult stem cells of skeletal muscle, are required for tissue repair throughout life. While many signaling pathways are known to control SC self-renewal, less is known about the mechanisms underlying the spatiotemporal control of self-renewal during skeletal muscle repair. Here, we m...

journal_title：Molecular biology of the cell

authors： Moyle LA,Cheng RY,Liu H,Davoudi S,Ferreira SA,Nissar AA,Sun Y,Gentleman E,Simmons CA,Gilbert PM

更新日期：2020-07-21 00:00:00

abstract：:Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal pep...

journal_title：Molecular biology of the cell

authors： Seemann J,Jokitalo EJ,Warren G

更新日期：2000-02-01 00:00:00

abstract：:The BNIP-2 and Cdc42GAP homology (BCH) domain is a novel regulator for Rho GTPases, but its impact on p50-Rho GTPase-activating protein (p50RhoGAP or Cdc42GAP) in cells remains elusive. Here we show that deletion of the BCH domain from p50RhoGAP enhanced its GAP activity and caused drastic cell rounding. Introducing c...

journal_title：Molecular biology of the cell

authors： Zhou YT,Chew LL,Lin SC,Low BC

更新日期：2010-09-15 00:00:00

abstract：:Protein kinase D2 (PKD2) belongs to the PKD family of serine/threonine kinases that is activated by phorbol esters and G protein-coupled receptors (GPCRs). Its C-terminal regulatory domain comprises two cysteine-rich domains (C1a/C1b) followed by a pleckstrin homology (PH) domain. Here, we examined the role of the reg...

journal_title：Molecular biology of the cell

authors： Auer A,von Blume J,Sturany S,von Wichert G,Van Lint J,Vandenheede J,Adler G,Seufferlein T

更新日期：2005-09-01 00:00:00

abstract：:Mammalian mitochondrial ribosomes contain two prokaryotic-like rRNAs, 12S and 16S, both encoded by mitochondrial DNA. As opposed to cytosolic ribosomes, however, these ribosomes are not thought to contain 5S rRNA. For this reason, it has been unclear whether 5S rRNA, which can be detected in mitochondrial preparations...

journal_title：Molecular biology of the cell

authors： Magalhães PJ,Andreu AL,Schon EA

更新日期：1998-09-01 00:00:00

abstract：:Cellular levels of the versatile second messenger cyclic (c)AMP are regulated by the antagonistic actions of the canonical G protein → adenylyl cyclase pathway that is initiated by G-protein-coupled receptors (GPCRs) and attenuated by phosphodiesterases (PDEs). Dysregulated cAMP signaling drives many diseases; for exa...

journal_title：Molecular biology of the cell

authors： Getz M,Swanson L,Sahoo D,Ghosh P,Rangamani P

更新日期：2019-06-15 00:00:00

abstract：:LLCPK-1 cells were transfected with a green fluorescent protein (GFP)-alpha tubulin construct and a cell line permanently expressing GFP-alpha tubulin was established (LLCPK-1alpha). The mitotic index and doubling time for LLCPK-1alpha were not significantly different from parental cells. Quantitative immunoblotting s...

journal_title：Molecular biology of the cell

authors： Rusan NM,Fagerstrom CJ,Yvon AM,Wadsworth P

更新日期：2001-04-01 00:00:00

abstract：:The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental question, we developed static and dynamic assays to follow exocytosis, endocytosis, and PM area changes during fibroblast sp...

journal_title：Molecular biology of the cell

authors： Gauthier NC,Rossier OM,Mathur A,Hone JC,Sheetz MP

更新日期：2009-07-01 00:00:00

abstract：:The human fungal pathogen Candida albicans can switch between yeast, pseudohyphal, and hyphal morphologies. To investigate whether the distinctive characteristics of hyphae are due to increased activity of the Cdc42 GTPase, strains lacking negative regulators of Cdc42 were constructed. Unexpectedly, the deletion of th...

journal_title：Molecular biology of the cell

authors： Court H,Sudbery P

更新日期：2007-01-01 00:00:00

abstract：:Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1) protects cells from oxidative stress via the base excision repair pathway and as a redox transcriptional coactivator. It is required for tumor progression/metastasis, and its up-regulation is associated with cancer resistance. Loss of APE1 expression causes ce...

journal_title：Molecular biology of the cell

authors： Vascotto C,Bisetto E,Li M,Zeef LA,D'Ambrosio C,Domenis R,Comelli M,Delneri D,Scaloni A,Altieri F,Mavelli I,Quadrifoglio F,Kelley MR,Tell G

更新日期：2011-10-01 00:00:00

abstract：:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes to...

journal_title：Molecular biology of the cell

authors： Saini SG,Liu C,Zhang P,Lee TH

更新日期：2014-12-01 00:00:00

abstract：:Phospholipase Ds (PLDs) are regulated enzymes that generate phosphatidic acid (PA), a putative second messenger implicated in the regulation of vesicular trafficking and cytoskeletal reorganization. Mast cells, when stimulated with antigen, show a dramatic alteration in their cytoskeleton and also release their secret...

journal_title：Molecular biology of the cell

authors： O'Luanaigh N,Pardo R,Fensome A,Allen-Baume V,Jones D,Holt MR,Cockcroft S

更新日期：2002-10-01 00:00:00

abstract：:Activation of the high-osmolarity glycerol (HOG) pathway for osmoregulation in the yeast Saccharomyces cerevisiae involves interaction of the adaptor Ste50p with the cytoplasmic tail of single-transmembrane protein Opy2p. We have determined the solution structure of the Ste50p-RA (Ras association) domain, and it shows...

journal_title：Molecular biology of the cell

authors： Ekiel I,Sulea T,Jansen G,Kowalik M,Minailiuc O,Cheng J,Harcus D,Cygler M,Whiteway M,Wu C

更新日期：2009-12-01 00:00:00

abstract：:S100A4, a member of the S100 family of Ca(2+)-binding proteins, is directly involved in tumor metastasis. In addition to its expression in tumor cells, S100A4 is expressed in normal cells and tissues, including fibroblasts and cells of the immune system. To examine the contribution of S100A4 to normal physiology, we e...

journal_title：Molecular biology of the cell

authors： Li ZH,Dulyaninova NG,House RP,Almo SC,Bresnick AR

更新日期：2010-08-01 00:00:00

abstract：:Ion transport in various tissues can be regulated by the cortical actin cytoskeleton. Specifically, involvement of actin dynamics in the regulation of nonvoltage-gated sodium channels has been shown. Herein, inside-out patch clamp experiments were performed to study the effect of the heterodimeric actin capping protei...

journal_title：Molecular biology of the cell

authors： Shumilina EV,Negulyaev YA,Morachevskaya EA,Hinssen H,Khaitlina SY

更新日期：2003-04-01 00:00:00

abstract：:Integrins are major receptors used by cells to interact with extracellular matrices. In this paper, we identify the first ligands for the beta 8 family of integrins, presenting evidence that integrin heterodimers containing the beta 8 subunit mediate interactions of chick sensory neurons with laminin-1, collagen IV, a...

journal_title：Molecular biology of the cell

authors： Venstrom K,Reichardt L

更新日期：1995-04-01 00:00:00

abstract：:Gaucher disease is a lysosomal storage disorder caused by a defect in the degradation of glucosylceramide catalyzed by the lysosomal enzyme β-glucocerebrosidase (GBA). GBA reaches lysosomes via association with its receptor, lysosomal integral membrane protein type 2 (LIMP-2). We found that distinct phosphatidylinosit...

journal_title：Molecular biology of the cell

authors： Jović M,Kean MJ,Szentpetery Z,Polevoy G,Gingras AC,Brill JA,Balla T

更新日期：2012-04-01 00:00:00

abstract：:Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phas...

journal_title：Molecular biology of the cell

authors： Bodor DL,Valente LP,Mata JF,Black BE,Jansen LE

更新日期：2013-04-01 00:00:00

abstract：:Eukaryotic cells actively block entry into mitosis in the presence of DNA damage or incompletely replicated DNA. This response is mediated by signal transduction cascades called cell cycle checkpoints. We show here that the human checkpoint control protein hRAD9 physically associates with two other checkpoint control ...

journal_title：Molecular biology of the cell

authors： St Onge RP,Udell CM,Casselman R,Davey S

更新日期：1999-06-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:The transcription factor Sp1 is ubiquitously expressed in different cells and thereby regulates the expression of genes involved in many cellular processes. This study reveals that Sp1 was phosphorylated during the mitotic stage in three epithelial tumor cell lines and one glioma cell line. By using different kinase i...

journal_title：Molecular biology of the cell

authors： Chuang JY,Wang YT,Yeh SH,Liu YW,Chang WC,Hung JJ

更新日期：2008-03-01 00:00:00

abstract：:Myc interacting zinc finger protein-1 (Miz1) is a transcription factor known to regulate cell cycle- and cell adhesion-related genes in cancer. Here we show that Miz1 also plays a critical role in neural crest development. In the chick, Miz1 is expressed throughout the neural plate and closing neural tube. Its morphol...

journal_title：Molecular biology of the cell

authors： Kerosuo L,Bronner ME

更新日期：2014-02-01 00:00:00

abstract：:We recently established an in vitro assay that monitors the fusion between latex-bead phagosomes and endocytic organelles in the presence of J774 macrophage cytosol (). Here, we show that different reagents affecting the actin cytoskeleton can either inhibit or stimulate this fusion process. Because the membranes of p...

journal_title：Molecular biology of the cell

authors： Jahraus A,Egeberg M,Hinner B,Habermann A,Sackman E,Pralle A,Faulstich H,Rybin V,Defacque H,Griffiths G

更新日期：2001-01-01 00:00:00

abstract：:Meiosis involves two successive rounds of chromosome segregation without an intervening S phase. Exit from meiosis I is distinct from mitotic exit, in that replication origins are not licensed by Mcm2-7 chromatin binding, but spindle disassembly occurs during a transient interphase-like state before meiosis II. The ab...

journal_title：Molecular biology of the cell

authors： Hua H,Namdar M,Ganier O,Gregan J,Méchali M,Kearsey SE

更新日期：2013-03-01 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:A chimeric protein (ERaeq) comprised of the invariant chain (Ii) of class II major histocompatability complex (MHC-II) and aequorin was localized in the endoplasmic reticulum (ER) of transfected human embryonal kidney 293 cells. The targeted aequorin resided in the lumen of the ER membrane system, including the nuclea...

journal_title：Molecular biology of the cell

authors： Button D,Eidsath A

更新日期：1996-03-01 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understo...

journal_title：Molecular biology of the cell

authors： Xie S,Mason FM,Martin AC

更新日期：2016-11-07 00:00:00

abstract：:The survival motor neuron (SMN) complex fulfils essential functions in the assembly of snRNPs, which are key components in the splicing of pre-mRNAs. Little is known about the regulation of SMN complex activity by posttranslational modification despite its complicated phosphorylation pattern. Several phosphatases had ...

journal_title：Molecular biology of the cell

authors： Husedzinovic A,Neumann B,Reymann J,Draeger-Meurer S,Chari A,Erfle H,Fischer U,Gruss OJ

更新日期：2015-01-15 00:00:00