Abstract:
:The membrane-anchored atlastin GTPase couples nucleotide hydrolysis to the catalysis of homotypic membrane fusion to form a branched endoplasmic reticulum network. Trans dimerization between atlastins anchored in opposing membranes, accompanied by a cross-over conformational change, is thought to draw the membranes together for fusion. Previous studies on the conformational coupling of atlastin to its GTP hydrolysis cycle have been carried out largely on atlastins lacking a membrane anchor. Consequently, whether fusion involves a discrete tethering step and, if so, the potential role of GTP hydrolysis and cross-over in tethering remain unknown. In this study, we used membrane-anchored atlastins in assays that separate tethering from fusion to dissect the requirements for each. We found that tethering depended on GTP hydrolysis, but, unlike fusion, it did not depend on cross-over. Thus GTP hydrolysis initiates stable head-domain contact in trans to tether opposing membranes, whereas cross-over formation plays a more pivotal role in powering the lipid rearrangements for fusion.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Saini SG,Liu C,Zhang P,Lee THdoi
10.1091/mbc.E14-08-1284subject
Has Abstractpub_date
2014-12-01 00:00:00pages
3942-53issue
24eissn
1059-1524issn
1939-4586pii
mbc.E14-08-1284journal_volume
25pub_type
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