Abstract:
:In vitro studies suggest that the Barren protein may function as an activator of DNA topoisomerase II and/or as a component of the Xenopus condensin complex. To better understand the role of Barren in vivo, we generated conditional alleles of the structural gene for Barren (BRN1) in Saccharomyces cerevisiae. We show that Barren is an essential protein required for chromosome condensation in vivo and that it is likely to function as an intrinsic component of the yeast condensation machinery. Consistent with this view, we show that Barren performs an essential function during a period of the cell cycle when chromosome condensation is established and maintained. In contrast, Barren does not serve as an essential activator of DNA topoisomerase II in vivo. Finally, brn1 mutants display additional phenotypes such as stretched chromosomes, aberrant anaphase spindles, and the accumulation of cells with >2C DNA content, suggesting that Barren function influences multiple aspects of chromosome transmission and dynamics.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Lavoie BD,Tuffo KM,Oh S,Koshland D,Holm Cdoi
10.1091/mbc.11.4.1293keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
1293-304issue
4eissn
1059-1524issn
1939-4586journal_volume
11pub_type
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