Abstract:
:The spatiotemporal dynamics of triglyceride (TG) storage in unilocular adipocytes are not well understood. Here we applied ex vivo technology to study trafficking and metabolism of fluorescent fatty acids in adipose tissue explants. Live imaging revealed multiple cytoplasmic nodules surrounding the large central lipid droplet (cLD) of unilocular adipocytes. Each cytoplasmic nodule harbors a series of closely associated cellular organelles, including micro-lipid droplets (mLDs), mitochondria, and the endoplasmic reticulum. Exogenously added free fatty acids are rapidly adsorbed by mLDs and concurrently get esterified to TG. This process is greatly accelerated by insulin. mLDs transfer their content to the cLD, serving as intermediates that mediate packaging of newly synthesized TG in the large interior of a unilocular adipocyte. This study reveals novel cell biological features that may contribute to the mechanism of adipocyte hypertrophy.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Chu M,Sampath H,Cahana DY,Kahl CA,Somwar R,Cornea A,Roberts CT Jr,Varlamov Odoi
10.1091/mbc.E14-06-1085subject
Has Abstractpub_date
2014-12-15 00:00:00pages
4096-105issue
25eissn
1059-1524issn
1939-4586pii
mbc.E14-06-1085journal_volume
25pub_type
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