The GPI transamidase complex of Saccharomyces cerevisiae contains Gaa1p, Gpi8p, and Gpi16p.

abstract：:Mathematical modeling has been instrumental in identifying common principles of cell polarity across diverse systems. These principles include positive feedback loops that are required to destabilize a spatially uniform state of the cell. The conserved small G-protein Cdc42 is a master regulator of eukaryotic cellular...

journal_title：Molecular biology of the cell

authors： Goryachev AB,Leda M

更新日期：2017-02-01 00:00:00

abstract：:Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

journal_title：Molecular biology of the cell

authors： Zhou D,Ursitti JA,Bloch RJ

更新日期：1998-01-01 00:00:00

abstract：:We conducted a genetic screen for mutations in myospheroid, the gene encoding the Drosophila betaPS integrin subunit, and identified point mutants in all of the structural domains of the protein. Surprisingly, we find that mutations in very strongly conserved residues will often allow sufficient integrin function to s...

journal_title：Molecular biology of the cell

authors： Jannuzi AL,Bunch TA,West RF,Brower DL

更新日期：2004-08-01 00:00:00

abstract：:Cell surface hemichannels (HCs) composed of different connexin (Cx) types are present in diverse cells and their possible role on FGF-1-induced cellular responses remains unknown. Here, we show that FGF-1 transiently (4-14 h, maximal at 7 h) increases the membrane permeability through HCs in HeLa cells expressing Cx43...

journal_title：Molecular biology of the cell

authors： Schalper KA,Palacios-Prado N,Retamal MA,Shoji KF,Martínez AD,Sáez JC

更新日期：2008-08-01 00:00:00

abstract：:After invasion of epithelial cells, Salmonella enterica Typhimurium resides within membrane-bound vacuoles where it survives and replicates. Like endocytic vesicles, the Salmonella-containing vacuoles (SCVs) undergo a maturation process that involves sequential acquisition of Rab5 and Rab7 and displacement toward the ...

journal_title：Molecular biology of the cell

authors： Harrison RE,Brumell JH,Khandani A,Bucci C,Scott CC,Jiang X,Finlay BB,Grinstein S

更新日期：2004-07-01 00:00:00

abstract：:Tumor necrosis factor α (TNF-α) receptor-associated factor 2 (TRAF2) regulates activation of the c-Jun N-terminal kinase (JNK)/c-Jun and the inhibitor of κB kinase (IKK)/nuclear factor κB (NF-κB) signaling cascades in response to TNF-α stimulation. Gene knockout studies have revealed that TRAF2 inhibits TNF-α-induced ...

journal_title：Molecular biology of the cell

authors： Zhang L,Blackwell K,Altaeva A,Shi Z,Habelhah H

更新日期：2011-01-01 00:00:00

abstract：:Transforming growth factor-β (TGF-β) receptor oligomerization has important roles in signaling. Complex formation among type I and type II (TβRI and TβRII) TGF-β receptors is well characterized and is essential for signal transduction. However, studies on their interactions with the type III TGF-β coreceptor (TβRIII) ...

journal_title：Molecular biology of the cell

authors： Tazat K,Hector-Greene M,Blobe GC,Henis YI

更新日期：2015-10-01 00:00:00

abstract：:The yeast Smf1p Nramp manganese transporter is posttranslationally regulated by environmental manganese. Smf1p is stabilized at the cell surface with manganese starvation, but is largely degraded in the vacuole with physiological manganese through a mechanism involving the Rsp5p adaptor complex Bsd2p/Tre1p/Tre2p. We n...

journal_title：Molecular biology of the cell

authors： Jensen LT,Carroll MC,Hall MD,Harvey CJ,Beese SE,Culotta VC

更新日期：2009-06-01 00:00:00

abstract：:An increase in the level of active, GTP-bound Ras is not necessary for transformation of chicken embryo fibroblasts (CEF) by v-Src. This suggests that other Ras-independent pathways contribute to transformation by v-Src. To address the possibility that activation of phosphatidylinositol-3-kinase (PI3K) and the mammali...

journal_title：Molecular biology of the cell

authors： Penuel E,Martin GS

更新日期：1999-06-01 00:00:00

abstract：:A primary cilium is found on most mammalian cells, where it acts as a cellular antenna for the reception of both mechanical and chemical signals. A variety of diseases are associated with defective ciliogenesis, reflecting the ubiquity of the function of cilia and the number of proteins required for their assembly. Pr...

journal_title：Molecular biology of the cell

authors： Copeland SJ,McRae A,Guarguaglini G,Trinkle-Mulcahy L,Copeland JW

更新日期：2018-07-01 00:00:00

abstract：:Calreticulin (CRT) is a high-capacity, low-affinity Ca2+-binding protein located in the lumen of the endoplasmic reticulum (ER) of all eukaryotic cells investigated so far. Its high level of conservation among different species suggests that it serves functions fundamental to cell survival. The role originally propose...

journal_title：Molecular biology of the cell

authors： Fasolato C,Pizzo P,Pozzan T

更新日期：1998-06-01 00:00:00

abstract：:The resistance of Candida albicans to many stresses is dependent on the stress-activated protein kinase (SAPK) Hog1. Hence we have explored the role of Hog1 in the regulation of transcriptional responses to stress. DNA microarrays were used to characterize the global transcriptional responses of HOG1 and hog1 cells to...

journal_title：Molecular biology of the cell

authors： Enjalbert B,Smith DA,Cornell MJ,Alam I,Nicholls S,Brown AJ,Quinn J

更新日期：2006-02-01 00:00:00

abstract：:Studies on physiological modulation of intercellular communication mediated by protein kinases are often complicated by the fact that cells express multiple gap junction proteins (connexins; Cx). Changes in cell coupling can be masked by simultaneous opposite regulation of the gap junction channel types expressed. We ...

journal_title：Molecular biology of the cell

authors： Kwak BR,Hermans MM,De Jonge HR,Lohmann SM,Jongsma HJ,Chanson M

更新日期：1995-12-01 00:00:00

abstract：:Myo1c is a member of the myosin superfamily that binds phosphatidylinositol-4,5-bisphosphate (PIP(2)), links the actin cytoskeleton to cellular membranes and plays roles in mechano-signal transduction and membrane trafficking. We located and characterized two distinct membrane binding sites within the regulatory and t...

journal_title：Molecular biology of the cell

authors： Hokanson DE,Laakso JM,Lin T,Sept D,Ostap EM

更新日期：2006-11-01 00:00:00

abstract：:We have identified five complementation groups of peroxisome biogenesis (peb) mutants in Saccharomyces cerevisiae by a positive selection procedure. Three of these contained morphologically recognizable peroxisomes, and two appeared to lack the organelle altogether. The packaging of peroxisomal proteins in these mutan...

journal_title：Molecular biology of the cell

authors： Zhang JW,Luckey C,Lazarow PB

更新日期：1993-12-01 00:00:00

abstract：:A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal ...

journal_title：Molecular biology of the cell

authors： Hut HM,Lemstra W,Blaauw EH,Van Cappellen GW,Kampinga HH,Sibon OC

更新日期：2003-05-01 00:00:00

abstract：:The AP-1 transcription factor JunD is highly expressed in intestinal epithelial cells, but its exact role in maintaining the integrity of intestinal epithelial barrier remains unknown. The tight junction (TJ) protein zonula occludens (ZO)-1 links the intracellular domain of TJ-transmembrane proteins occludin, claudins...

journal_title：Molecular biology of the cell

authors： Chen J,Xiao L,Rao JN,Zou T,Liu L,Bellavance E,Gorospe M,Wang JY

更新日期：2008-09-01 00:00:00

abstract：:Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. Claspin associates with replication forks upon origin unwinding. We show that Claspin contains a replication fork-interacting domain (RFID, residues 265-605) that associates with Cdc45, DNA pol...

journal_title：Molecular biology of the cell

authors： Lee J,Gold DA,Shevchenko A,Shevchenko A,Dunphy WG

更新日期：2005-11-01 00:00:00

abstract：:Cystic fibrosis results from mutations in the cystic fibrosis conductance regulator protein (CFTR), a cAMP/protein kinase A (PKA) and ATP-regulated Cl(-) channel. CFTR is increasingly recognized as a component of multiprotein complexes and although several inhibitory proteins to CFTR have been identified, protein comp...

journal_title：Molecular biology of the cell

authors： Borthwick LA,McGaw J,Conner G,Taylor CJ,Gerke V,Mehta A,Robson L,Muimo R

更新日期：2007-09-01 00:00:00

abstract：:Telomere dysfunction results in fertility defects in a number of organisms. Although data from fission yeast and Caenorhabditis elegans suggests that telomere dysfunction manifests itself primarily as defects in proper meiotic chromosome segregation, it is unclear how mammalian telomere dysfunction results in germ cel...

journal_title：Molecular biology of the cell

authors： Hemann MT,Rudolph KL,Strong MA,DePinho RA,Chin L,Greider CW

更新日期：2001-07-01 00:00:00

abstract：:The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio a...

journal_title：Molecular biology of the cell

authors： Chan K,Goldmark JP,Roth MB

更新日期：2010-07-01 00:00:00

abstract：:Dynamic regulation of cytoskeletal contractility through phosphorylation of the nonmuscle Myosin-II regulatory light chain (MRLC) provides an essential source of tension for shaping epithelial tissues. Rho GTPase and its effector kinase ROCK have been implicated in regulating MRLC phosphorylation in vivo, but evidence...

journal_title：Molecular biology of the cell

authors： Neubueser D,Hipfner DR

更新日期：2010-08-15 00:00:00

abstract：:We have shown that ouabain activates Src, resulting in subsequent tyrosine phosphorylation of multiple effectors. Here, we tested if the Na+/K+-ATPase and Src can form a functional signaling complex. In LLC-PK1 cells the Na+/K+-ATPase and Src colocalized in the plasma membrane. Fluorescence resonance energy transfer a...

journal_title：Molecular biology of the cell

authors： Tian J,Cai T,Yuan Z,Wang H,Liu L,Haas M,Maksimova E,Huang XY,Xie ZJ

更新日期：2006-01-01 00:00:00

abstract：:The [URE3] and [PSI(+)] prions are infectious amyloid forms of Ure2p and Sup35p. Several chaperones influence prion propagation: Hsp104p overproduction destabilizes [PSI(+)], whereas [URE3] is sensitive to excess of Ssa1p or Ydj1p. Here, we show that overproduction of the chaperone, Sse1p, can efficiently cure [URE3]....

journal_title：Molecular biology of the cell

authors： Kryndushkin D,Wickner RB

更新日期：2007-06-01 00:00:00

abstract：:In animal cells, positioning of the mitotic spindle is crucial for defining the plane of cytokinesis and the size ratio of daughter cells. We have characterized this phenomenon in a rat epithelial cell line using microscopy, micromanipulation, and microinjection. Unmanipulated cells position the mitotic spindle near t...

journal_title：Molecular biology of the cell

authors： O'Connell CB,Wang YL

更新日期：2000-05-01 00:00:00

abstract：:α-Arrestins play a key role as trafficking adaptors in both yeast and mammals. The yeast Rim8/Art9 α-arrestin mediates the recruitment of endosomal sorting complex required for transport (ESCRT) to the seven-transmembrane protein Rim21 in the ambient pH signaling RIM pathway. ESCRT is believed to function as a signali...

journal_title：Molecular biology of the cell

authors： Herrador A,Livas D,Soletto L,Becuwe M,Léon S,Vincent O

更新日期：2015-06-01 00:00:00

abstract：:The intermembrane space of mitochondria accommodates the essential mitochondrial intermembrane space assembly (MIA) machinery that catalyzes oxidative folding of proteins. The disulfide bond formation pathway is based on a relay of reactions involving disulfide transfer from the sulfhydryl oxidase Erv1 to Mia40 and fr...

journal_title：Molecular biology of the cell

authors： Böttinger L,Gornicka A,Czerwik T,Bragoszewski P,Loniewska-Lwowska A,Schulze-Specking A,Truscott KN,Guiard B,Milenkovic D,Chacinska A

更新日期：2012-10-01 00:00:00

abstract：:Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

journal_title：Molecular biology of the cell

authors： Mohi MG,Arai Ki,Watanabe S

更新日期：1998-12-01 00:00:00

abstract：:In view of the common regulatory mechanism that induces transcription of the mitotic phosphatase cdc25C and cyclin A at the beginning of S-phase, we investigated whether cdc25C was required for S-phase transit. Here, we show that in both nontransformed human fibroblasts and HeLa cells, cdc25C protein levels significan...

journal_title：Molecular biology of the cell

authors： Turowski P,Franckhauser C,Morris MC,Vaglio P,Fernandez A,Lamb NJ

更新日期：2003-07-01 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00