Abstract:
:Endoplasmic reticulum-localized DnaJ 4 (ERdj4) is an immunoglobulin-binding protein (BiP) cochaperone and component of the endoplasmic reticulum-associated degradation (ERAD) pathway that functions to remove unfolded/misfolded substrates from the ER lumen under conditions of ER stress. To elucidate the function of ERdj4 in vivo, we disrupted the ERdj4 locus using gene trap (GT) mutagenesis, leading to hypomorphic expression of ERdj4 in mice homozygous for the trapped allele (ERdj4(GT/GT)). Approximately half of ERdj4(GT/GT) mice died perinatally associated with fetal growth restriction, reduced hepatic glycogen stores, and hypoglycemia. Surviving adult mice exhibited evidence of constitutive ER stress in multiple cells/tissues, including fibroblasts, lung, kidney, salivary gland, and pancreas. Elevated ER stress in pancreatic β cells of ERdj4(GT/GT) mice was associated with β cell loss, hypoinsulinemia, and glucose intolerance. Collectively these results suggest an important role for ERdj4 in maintaining ER homeostasis during normal fetal growth and postnatal adaptation to metabolic stress.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Fritz JM,Dong M,Apsley KS,Martin EP,Na CL,Sitaraman S,Weaver TEdoi
10.1091/mbc.E13-06-0319subject
Has Abstractpub_date
2014-02-01 00:00:00pages
431-40issue
4eissn
1059-1524issn
1939-4586pii
mbc.E13-06-0319journal_volume
25pub_type
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