Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.

abstract：:In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...

journal_title：Molecular biology of the cell

authors： Deaton JD,Guerrero T,Howard TH

更新日期：1992-12-01 00:00:00

abstract：:Budding yeast treated with hydroxyurea (HU) activate the S phase checkpoint kinase Rad53, which prevents DNA replication forks from undergoing aberrant structural transitions and nuclease processing. Rad53 is also required to prevent premature extension of the mitotic spindle that assembles during a HU-extended S phas...

journal_title：Molecular biology of the cell

authors： Julius J,Peng J,McCulley A,Caridi C,Arnak R,See C,Nugent CI,Feng W,Bachant J

更新日期：2019-10-15 00:00:00

abstract：:Cell surface hemichannels (HCs) composed of different connexin (Cx) types are present in diverse cells and their possible role on FGF-1-induced cellular responses remains unknown. Here, we show that FGF-1 transiently (4-14 h, maximal at 7 h) increases the membrane permeability through HCs in HeLa cells expressing Cx43...

journal_title：Molecular biology of the cell

authors： Schalper KA,Palacios-Prado N,Retamal MA,Shoji KF,Martínez AD,Sáez JC

更新日期：2008-08-01 00:00:00

abstract：:MAGP-1 and fibrillin-1, two protein components of extracellular microfibrils, were shown by immunoprecipitation studies to interact with the chondroitin sulfate proteoglycan decorin in the medium of cultured fetal bovine chondrocytes. Decorin interacted with each protein individually and with both proteins together to...

journal_title：Molecular biology of the cell

authors： Trask BC,Trask TM,Broekelmann T,Mecham RP

更新日期：2000-05-01 00:00:00

abstract：:More than 97% of mice in which the C-terminal region of connexin43 (Cx43) was removed (designated as Cx43K258stop) die shortly after birth due to a defect of the epidermal barrier. The abnormal expression of Cx43K258stop protein in the uppermost layers of the epidermis seems to perturb terminal differentiation of kera...

journal_title：Molecular biology of the cell

authors： Maass K,Ghanem A,Kim JS,Saathoff M,Urschel S,Kirfel G,Grümmer R,Kretz M,Lewalter T,Tiemann K,Winterhager E,Herzog V,Willecke K

更新日期：2004-10-01 00:00:00

abstract：:Keratins are cytoplasmic intermediate filament proteins preferentially expressed by epithelial tissues in a site-specific and differentiation-dependent manner. The complex network of keratin filaments in stratified epithelia is tightly regulated during squamous cell differentiation. Keratin 14 (K14) is expressed in mi...

journal_title：Molecular biology of the cell

authors： Alam H,Sehgal L,Kundu ST,Dalal SN,Vaidya MM

更新日期：2011-11-01 00:00:00

abstract：:Chironomus tentans-repressor splicing factor (Ct-RSF) represses the activation of splicing by SR proteins in vitro. Ct-RSF colocalizes with the Ser-Arg-rich (SR) protein hrp45 in interchromatin granule clusters and coimmunoprecipitates with hrp45 in nuclear extracts. Ct-RSF and hrp45 can also interact directly in vitr...

journal_title：Molecular biology of the cell

authors： Björk P,Wetterberg-Strandh I,Baurén G,Wieslander L

更新日期：2006-01-01 00:00:00

abstract：:Lactogenic hormones and extracellular matrix (ECM) act synergistically to regulate beta-casein expression in culture. We have developed a functional subpopulation of the mouse mammary epithelial cell strain COMMA-1D (designated CID 9), which expresses high level of beta-casein, forms alveolar-like structures when plat...

journal_title：Molecular biology of the cell

authors： Schmidhauser C,Casperson GF,Myers CA,Sanzo KT,Bolten S,Bissell MJ

更新日期：1992-06-01 00:00:00

abstract：:Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p lo...

journal_title：Molecular biology of the cell

authors： Pollard LW,Onishi M,Pringle JR,Lord M

更新日期：2012-07-01 00:00:00

abstract：:Severe heat stress causes protein denaturation in various cellular compartments. If Saccharomyces cerevisiae cells grown at 24 degrees C are preconditioned at 37 degrees C, proteins denatured by subsequent exposure to 48-50 degrees C can be renatured when the cells are allowed to recover at 24 degrees C. Conformationa...

journal_title：Molecular biology of the cell

authors： Hänninen AL,Simola M,Saris N,Makarow M

更新日期：1999-11-01 00:00:00

abstract：:Programmed DNA rearrangements are important processes present in many organisms. In the ciliated protozoan Tetrahymena thermophila, DNA rearrangements occur during the sexual conjugation process and lead to the deletion of thousands of specific DNA segments and fragmentation of the chromosomes. In this study, we found...

journal_title：Molecular biology of the cell

authors： Lin IT,Chao JL,Yao MC

更新日期：2012-06-01 00:00:00

abstract：:The gamma-tubulin ring complex (gammaTuRC), consisting of multiple protein subunits, can nucleate microtubule assembly. Although many subunits of the gammaTuRC have been identified, a complete set remains to be defined in any organism. In addition, how the subunits interact with each other to assemble into gammaTuRC r...

journal_title：Molecular biology of the cell

authors： Gunawardane RN,Martin OC,Zheng Y

更新日期：2003-03-01 00:00:00

abstract：:The Rho family of GTP-binding proteins plays a critical role in a variety of cellular processes, including cytoskeletal reorganization and activation of kinases such as p38 and C-jun N-terminal kinase (JNK) MAPKs. We report here that dominant negative forms of Rac1 and Cdc42Hs inhibit the expression of the muscle-spec...

journal_title：Molecular biology of the cell

authors： Meriane M,Roux P,Primig M,Fort P,Gauthier-Rouvière C

更新日期：2000-08-01 00:00:00

abstract：:In endothelial cells specifically, cPLA2alpha translocates from the cytoplasm to the Golgi complex in response to cell confluence. Considering the link between confluence and cell-cell junction formation, and the emerging role of cPLA2alpha in intracellular trafficking, we tested whether Golgi-associated cPLA2alpha is...

journal_title：Molecular biology of the cell

authors： Regan-Klapisz E,Krouwer V,Langelaar-Makkinje M,Nallan L,Gelb M,Gerritsen H,Verkleij AJ,Post JA

更新日期：2009-10-01 00:00:00

abstract：:Doing scientific research can be a challenging, but creative and rewarding career. However, the challenge of doing research is orders of magnitude more difficult for scientists lacking access to the necessary resources. Resource-scarce environments are quite common worldwide and are highly contingent on the financial ...

journal_title：Molecular biology of the cell

authors： Karasmanis EP

更新日期：2018-06-01 00:00:00

abstract：:Autophagy is a conserved eukaryotic pathway critical for cellular adaptation to changes in nutrition levels and stress. The class III phosphatidylinositol (PI)3-kinase complexes I and II (PI3KC3-C1 and -C2) are essential for autophagosome initiation and maturation, respectively, from highly curved vesicles. We used a ...

journal_title：Molecular biology of the cell

authors： Brier LW,Ge L,Stjepanovic G,Thelen AM,Hurley JH,Schekman R

更新日期：2019-04-15 00:00:00

abstract：:Outer dynein arms (ODAs) are multiprotein complexes that drive flagellar beating. Based on genetic and biochemical analyses, ODAs preassemble in the cell body and then move into the flagellum by intraflagellar transport (IFT). To study ODA transport in vivo, we expressed the essential intermediate chain 2 tagged with ...

journal_title：Molecular biology of the cell

authors： Dai J,Barbieri F,Mitchell DR,Lechtreck KF

更新日期：2018-10-15 00:00:00

abstract：:A symposium was held at the University of California, San Diego, to honor the contributions of Nobel Laureate, George Palade, to cell biology. The speakers included Günter Blobel, on the structure and function of nuclear pore complexes; Peter Walter, on the unfolded protein response in health and disease; Randy Schekm...

journal_title：Molecular biology of the cell

authors： Schmid SL,Farquhar MG

更新日期：2010-07-15 00:00:00

abstract：:Cholesterol regulates plasma membrane (PM) association and functioning of syntaxin-4 and soluble N-ethylmaleimide-sensitive fusion protein 23 (SNAP23) in the secretory pathway. However, the molecular mechanism and cellular cholesterol pools that determine the localization and assembly of these target membrane SNAP rec...

journal_title：Molecular biology of the cell

authors： Reverter M,Rentero C,de Muga SV,Alvarez-Guaita A,Mulay V,Cairns R,Wood P,Monastyrskaya K,Pol A,Tebar F,Blasi J,Grewal T,Enrich C

更新日期：2011-11-01 00:00:00

abstract：:Cell morphogenesis depends on polarized exocytosis. One widely held model posits that long-range transport and exocyst-dependent tethering of exocytic vesicles at the plasma membrane sequentially drive this process. Here, we describe that disruption of either actin-based long-range transport and microtubules or the ex...

journal_title：Molecular biology of the cell

authors： Bendezú FO,Martin SG

更新日期：2011-01-01 00:00:00

abstract：:ATP8A2 is a P4-ATPase that flips phosphatidylserine and phosphatidylethanolamine across cell membranes. This generates membrane phospholipid asymmetry, a property important in many cellular processes, including vesicle trafficking. ATP8A2 deficiency causes severe neurodegenerative diseases. We investigated the role of...

journal_title：Molecular biology of the cell

authors： Chalat M,Moleschi K,Molday RS

更新日期：2017-02-01 00:00:00

abstract：:Rapid β2-integrin activation is indispensable for leukocyte adhesion and recruitment to sites of infection and is mediated by chemokine- or P-selectin glycoprotein ligand-1-induced inside-out signaling. Here we uncovered a novel pathway for rapid activation of integrin-dependent leukocyte adhesion, triggered by toll-l...

journal_title：Molecular biology of the cell

authors： Chung KJ,Mitroulis I,Wiessner JR,Zheng YY,Siegert G,Sperandio M,Chavakis T

更新日期：2014-10-01 00:00:00

abstract：:Little is known about the mechanisms that determine localization of proteins to the plasma membrane in Saccharomyces cerevisiae. The length of the transmembrane domains and association of proteins with lipid rafts have been proposed to play a role in sorting to the cell surface. Here, we report that Fus1p, an O-glycos...

journal_title：Molecular biology of the cell

authors： Proszynski TJ,Simons K,Bagnat M

更新日期：2004-04-01 00:00:00

abstract：:The mitochondrial cytochrome c oxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and...

journal_title：Molecular biology of the cell

authors： Bareth B,Nikolov M,Lorenzi I,Hildenbeutel M,Mick DU,Helbig C,Urlaub H,Ott M,Rehling P,Dennerlein S

更新日期：2016-05-15 00:00:00

abstract：:Regulated exocytosis is thought to occur either by "full fusion," where the secretory vesicle fuses with the plasma membrane (PM) via a fusion pore that then dilates until the secretory vesicle collapses into the PM; or by "kiss-and-run," where the fusion pore does not dilate and instead rapidly reseals such that the ...

journal_title：Molecular biology of the cell

authors： Sokac AM,Bement WM

更新日期：2006-04-01 00:00:00

abstract：:The small G proteins Cdc42, Rac1, and Rac2 regulate the rearrangements of actin and membrane necessary for Fcgamma receptor-mediated phagocytosis by macrophages. Activated, GTP-bound Cdc42, Rac1, and Rac2 bind to the p21-binding domain (PBD) of PAK1, and this interaction provided a basis for microscopic methods to loc...

journal_title：Molecular biology of the cell

authors： Hoppe AD,Swanson JA

更新日期：2004-08-01 00:00:00

abstract：:Spa2p is a nonessential protein that regulates yeast cell polarity. It localizes early to the presumptive bud site and remains at sites of growth throughout the cell cycle. To understand how Spa2p localization is regulated and to gain insight into its molecular function in cell polarity, we used a coimmunoprecipitatio...

journal_title：Molecular biology of the cell

authors： Shih JL,Reck-Peterson SL,Newitt R,Mooseker MS,Aebersold R,Herskowitz I

更新日期：2005-10-01 00:00:00

abstract：:end4-1 was isolated as a temperature-sensitive endocytosis mutant. We cloned and sequenced END4 and found that it is identical to SLA2/MOP2. This gene is required for growth at high temperature, viability in the absence of Abp1p, polarization of the cortical actin cytoskeleton, and endocytosis. We used a mutational an...

journal_title：Molecular biology of the cell

authors： Wesp A,Hicke L,Palecek J,Lombardi R,Aust T,Munn AL,Riezman H

更新日期：1997-11-01 00:00:00

abstract：:Stem cells are classically defined by their multipotent, long-term proliferation, and self-renewal capabilities. Here, we show that increased antioxidant capacity represents an additional functional characteristic of muscle-derived stem cells (MDSCs). Seeking to understand the superior regenerative capacity of MDSCs c...

journal_title：Molecular biology of the cell

authors： Urish KL,Vella JB,Okada M,Deasy BM,Tobita K,Keller BB,Cao B,Piganelli JD,Huard J

更新日期：2009-01-01 00:00:00

abstract：:Multiple tau gene mutations are pathogenic for hereditary frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), with filamentous tau aggregates as the major lesions in the CNS of these patients. Recent studies have shown that bacterially expressed recombinant tau proteins with FTDP-17 missense mu...

journal_title：Molecular biology of the cell

authors： Vogelsberg-Ragaglia V,Bruce J,Richter-Landsberg C,Zhang B,Hong M,Trojanowski JQ,Lee VM

更新日期：2000-12-01 00:00:00