Critical activities of Rac1 and Cdc42Hs in skeletal myogenesis: antagonistic effects of JNK and p38 pathways.

abstract：:During organogenesis, different cell types need to work together to generate functional multicellular structures. To study this process, we made use of the genetically tractable fly retina, with a focus on the mechanisms that coordinate morphogenesis between the different epithelial cell types that make up the optical...

journal_title：Molecular biology of the cell

authors： Blackie L,Walther RF,Staddon MF,Banerjee S,Pichaud F

更新日期：2020-06-15 00:00:00

abstract：:Overexpression of the inhibitory Smad, Smad7, is used frequently to implicate the Smad pathway in cellular responses to transforming growth factor beta (TGF-beta) signaling; however, Smad7 regulates several other proteins, including Cdc42, p38MAPK, and beta-catenin. We report an alternative approach for more specifica...

journal_title：Molecular biology of the cell

authors： Zhao BM,Hoffmann FM

更新日期：2006-09-01 00:00:00

abstract：:14-3-3 proteins via binding serine/threonine-phosphorylated proteins regulate diverse intracellular processes in all eukaryotic organisms. Here, we examine the role of 14-3-3 self-dimerization in target binding, and in the susceptibility of 14-3-3 to undergo phosphorylation. Using a phospho-specific antibody developed...

journal_title：Molecular biology of the cell

authors： Shen YH,Godlewski J,Bronisz A,Zhu J,Comb MJ,Avruch J,Tzivion G

更新日期：2003-11-01 00:00:00

abstract：:Alzheimer's β-amyloid precursor protein (APP) associates with kinesin-1 via JNK-interacting protein 1 (JIP1); however, the role of JIP1 in APP transport by kinesin-1 in neurons remains unclear. We performed a quantitative analysis to understand the role of JIP1 in APP axonal transport. In JIP1-deficient neurons, we fi...

journal_title：Molecular biology of the cell

authors： Chiba K,Araseki M,Nozawa K,Furukori K,Araki Y,Matsushima T,Nakaya T,Hata S,Saito Y,Uchida S,Okada Y,Nairn AC,Davis RJ,Yamamoto T,Kinjo M,Taru H,Suzuki T

更新日期：2014-11-05 00:00:00

abstract：:Pro-alpha-factor (pro-alphaf) is posttranslationally modified in the yeast Golgi complex by the addition of alpha1,6-, alpha1,2-, and alpha1,3-linked mannose to N-linked oligosaccharides and by a Kex2p-initiated proteolytic processing event. Previous work has indicated that the alpha1,6- and alpha1,3-mannosylation and...

journal_title：Molecular biology of the cell

authors： Brigance WT,Barlowe C,Graham TR

更新日期：2000-01-01 00:00:00

abstract：:Mechanosensing describes the ability of a cell to sense mechanical cues of its microenvironment, including not only all components of force, stress, and strain but also substrate rigidity, topology, and adhesiveness. This ability is crucial for the cell to respond to the surrounding mechanical cues and adapt to the ch...

journal_title：Molecular biology of the cell

authors： Chen Y,Ju L,Rushdi M,Ge C,Zhu C

更新日期：2017-11-07 00:00:00

abstract：:All known mechanisms of mitotic spindle orientation rely on astral microtubules. We report that even in the absence of astral microtubules, metaphase spindles in MDCK and HeLa cells are not randomly positioned along their x-z dimension, but preferentially adopt shallow β angles between spindle pole axis and substratum...

journal_title：Molecular biology of the cell

authors： Lázaro-Diéguez F,Ispolatov I,Müsch A

更新日期：2015-04-01 00:00:00

abstract：:Cell emergence onto damaged or organized fibrous extracellular matrix (ECM) is a crucial precursor to collective cell migration in wound closure and cancer metastasis, respectively. However, there is a fundamental gap in our quantitative understanding of the role of local ECM size and arrangement in cell emergence-bas...

journal_title：Molecular biology of the cell

authors： Sharma P,Ng C,Jana A,Padhi A,Szymanski P,Lee JSH,Behkam B,Nain AS

更新日期：2017-09-15 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF) is an incurable disease of the lung that is characterized by excessive deposition of extracellular matrix (ECM), resulting in disruption of normal lung function. The signals regulating fibrosis include both transforming growth factor beta (TGF-β) and tissue rigidity and a major sign...

journal_title：Molecular biology of the cell

authors： Monaghan-Benson E,Wittchen ES,Doerschuk CM,Burridge K

更新日期：2018-09-01 00:00:00

abstract：:The serotonin (5-hydroxytryptamine; 5-HT)(2C) receptor is a G protein-coupled receptor (GPCR) exclusively expressed in CNS that has been implicated in numerous brain disorders, including anxio-depressive states. Like many GPCRs, 5-HT(2C) receptors physically interact with a variety of intracellular proteins in additio...

journal_title：Molecular biology of the cell

authors： Labasque M,Reiter E,Becamel C,Bockaert J,Marin P

更新日期：2008-11-01 00:00:00

abstract：:The assembly of four soluble N-ethylmaleimide-sensitive factor attachment protein receptor domains into a complex is essential for membrane fusion. In most cases, the four SNARE-domains are encoded by separate membrane-targeted proteins. However, in the exocytotic pathway, two SNARE-domains are present in one protein,...

journal_title：Molecular biology of the cell

authors： Nagy G,Milosevic I,Mohrmann R,Wiederhold K,Walter AM,Sørensen JB

更新日期：2008-09-01 00:00:00

abstract：:CD98, an early marker of T-cell activation, is an important regulator of integrin-mediated adhesion events. Previous studies suggest that CD98 is coupled to both cellular activation and transformation and is involved in the pathogenesis of viral infection, inflammatory disease, and cancer. Understanding of the molecul...

journal_title：Molecular biology of the cell

authors： Rintoul RC,Buttery RC,Mackinnon AC,Wong WS,Mosher D,Haslett C,Sethi T

更新日期：2002-08-01 00:00:00

abstract：:Notch and neurotrophins control neuronal shape, but it is not known whether their signaling pathways intersect. Here we report results from hippocampal neuronal cultures that are in support of this possibility. We found that low cell density or blockade of Notch signaling by a soluble Delta-Fc ligand decreased the mRN...

journal_title：Molecular biology of the cell

authors： Salama-Cohen P,Arévalo MA,Meier J,Grantyn R,Rodríguez-Tébar A

更新日期：2005-01-01 00:00:00

abstract：:Synaptosomal associated protein of 23 kDa (SNAP-23), a plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE), has been implicated in phagocytosis by macrophages. For elucidation of its precise role in this process, a macrophage line overexpressing monomeric Venus-tagge...

journal_title：Molecular biology of the cell

authors： Sakurai C,Hashimoto H,Nakanishi H,Arai S,Wada Y,Sun-Wada GH,Wada I,Hatsuzawa K

更新日期：2012-12-01 00:00:00

abstract：:Dynamics of endocytic clathrin-coated structures can be remarkably divergent across different cell types, cells within the same culture, or even distinct surfaces of the same cell. The origin of this astounding heterogeneity remains to be elucidated. Here we show that cellular processes associated with changes in effe...

journal_title：Molecular biology of the cell

authors： Willy NM,Ferguson JP,Huber SD,Heidotting SP,Aygün E,Wurm SA,Johnston-Halperin E,Poirier MG,Kural C

更新日期：2017-11-15 00:00:00

abstract：:ER quality control (ERQC) prevents the exit of misfolded secretory and membrane proteins from the ER. A critical aspect of ERQC is a transcriptional response called the unfolded protein response (UPR), which up-regulates genes that enable cells to cope with misfolded, ER-retained proteins. In this study, we compare th...

journal_title：Molecular biology of the cell

authors： Metzger MB,Michaelis S

更新日期：2009-02-01 00:00:00

abstract：:How nuclear size is regulated is a fundamental cell-biological question with relevance to cancers, which often exhibit enlarged nuclei. We previously reported that conventional protein kinase C (cPKC) contributes to nuclear size reductions that occur during early Xenopus development. Here we report that PKC-mediated p...

journal_title：Molecular biology of the cell

authors： Edens LJ,Dilsaver MR,Levy DL

更新日期：2017-05-15 00:00:00

abstract：:B-type cyclins are rapidly degraded at the transition between metaphase and anaphase and their ubiquitin-mediated proteolysis is required for cells to exit mitosis. We used a novel enrichment to isolate new budding mutants that arrest the cell cycle in mitosis. Most of these mutants lie in the CDC16, CDC23, and CDC27 ...

journal_title：Molecular biology of the cell

authors： Hwang LH,Murray AW

更新日期：1997-10-01 00:00:00

abstract：:Endomitosis is a special type of mitosis in which only cytokinesis-the final step of the cell division cycle-is defective, resulting in polyploid cells. Although endomitosis is biologically important, its regulatory aspects remain elusive. Psychosine, a lysogalactosylceramide, prevents proper cytokinesis when suppleme...

journal_title：Molecular biology of the cell

authors： Watanabe H,Okahara K,Naito-Matsui Y,Abe M,Go S,Inokuchi J,Okazaki T,Kobayashi T,Kozutsumi Y,Oka S,Takematsu H

更新日期：2016-07-01 00:00:00

abstract：:The key regulator of entry into mitosis is the serine/threonine kinase p34cdc2. This kinase is regulated both by association with cyclins and by phosphorylation at several sites. Phosphorylation at Tyr 15 and Thr 14 are believed to inhibit the kinase activity of cdc2. In Schizosaccharomyces pombe, the wee1 (and possib...

journal_title：Molecular biology of the cell

authors： Kornbluth S,Sebastian B,Hunter T,Newport J

更新日期：1994-03-01 00:00:00

abstract：:The NUDF protein of the filamentous fungus Aspergillus nidulans functions in the cytoplasmic dynein pathway. It binds several proteins, including the NUDE protein. Green fluorescent protein-tagged NUDF and NUDA (dynein heavy chain) localize to linearly moving dashes ("comets") that coincide with microtubule ends. Here...

journal_title：Molecular biology of the cell

authors： Efimov VP

更新日期：2003-03-01 00:00:00

abstract：:cAR1, a G protein-coupled receptor (GPCR) for cAMP, is required for the multicellular development of Dictyostelium. The activation of multiple pathways by cAR1 is transient because of poorly defined adaptation mechanisms. To investigate this, we used a genetic screen for impaired development to isolate four dominant-n...

journal_title：Molecular biology of the cell

authors： Zhang M,Goswami M,Hereld D

更新日期：2005-02-01 00:00:00

abstract：:The BNIP-2 and Cdc42GAP homology (BCH) domain is a novel regulator for Rho GTPases, but its impact on p50-Rho GTPase-activating protein (p50RhoGAP or Cdc42GAP) in cells remains elusive. Here we show that deletion of the BCH domain from p50RhoGAP enhanced its GAP activity and caused drastic cell rounding. Introducing c...

journal_title：Molecular biology of the cell

authors： Zhou YT,Chew LL,Lin SC,Low BC

更新日期：2010-09-15 00:00:00

abstract：:In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...

journal_title：Molecular biology of the cell

authors： Deaton JD,Guerrero T,Howard TH

更新日期：1992-12-01 00:00:00

abstract：:Previous studies have shown that in a given cell type, certain active genes associate with SC-35 domains, nuclear regions rich in RNA metabolic factors and excluded from heterochromatin. This organization is not seen for all active genes; therefore, it is important to determine whether and when this locus-specific org...

journal_title：Molecular biology of the cell

authors： Moen PT Jr,Johnson CV,Byron M,Shopland LS,de la Serna IL,Imbalzano AN,Lawrence JB

更新日期：2004-01-01 00:00:00

abstract：:Golgi fragmentation is a common feature in multiple neurodegenerative diseases; however, the precise mechanism that causes fragmentation remains obscure. A potential link between Cdk5 and Golgi fragmentation in Alzheimer's disease (AD) was investigated in this study. Because Golgi is physiologically fragmented during ...

journal_title：Molecular biology of the cell

authors： Sun KH,de Pablo Y,Vincent F,Johnson EO,Chavers AK,Shah K

更新日期：2008-07-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:EB1 is a conserved microtubule plus end tracking protein considered to play crucial roles in microtubule organization and the interaction of microtubules with the cell cortex. Despite intense studies carried out in yeast and cultured cells, the role of EB1 in multicellular systems remains to be elucidated. Here, we de...

journal_title：Molecular biology of the cell

authors： Elliott SL,Cullen CF,Wrobel N,Kernan MJ,Ohkura H

更新日期：2005-02-01 00:00:00

abstract：:MicroRNAs (miRNAs), the tiny regulatory RNAs, form complexes with Argonaute (Ago) proteins and inhibit gene expression in metazoan cells. While studying parasite-invaded macrophages, we identify a unique mode of gene regulation in which the parasite Leishmania donovani (Ld) causes mitochondrial depolarization, reduces...

journal_title：Molecular biology of the cell

authors： Chakrabarty Y,Bhattacharyya SN

更新日期：2017-07-15 00:00:00

abstract：:We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation...

journal_title：Molecular biology of the cell

authors： Kuragano M,Uyeda TQP,Kamijo K,Murakami Y,Takahashi M

更新日期：2018-04-15 00:00:00