The Palade symposium: celebrating cell biology at its best.

abstract：:The tumor suppressor protein p53 mediates stress-induced growth arrest or apoptosis and plays a major role in safeguarding genome integrity. In response to DNA damage, p53 can be modified at multiple sites by phosphorylation and acetylation. We report on the characterization of p53 C-terminal phosphorylation by CHK1 a...

journal_title：Molecular biology of the cell

authors： Ou YH,Chung PH,Sun TP,Shieh SY

更新日期：2005-04-01 00:00:00

abstract：:Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. However, the specific functional contributions of the catenins to the establishment of strong adhesion are not fully understood. Here we use bi...

journal_title：Molecular biology of the cell

authors： Oas RG,Nanes BA,Esimai CC,Vincent PA,García AJ,Kowalczyk AP

更新日期：2013-03-01 00:00:00

abstract：:Scientific communication, career advancement, and funding decisions are all dependent on research publications. The way manuscripts are handled by high-visibility, professionally edited magazines differs from the way academic journals evaluate manuscripts, using active scientists as monitoring editors. In this essay, ...

journal_title：Molecular biology of the cell

authors： Bretscher A

更新日期：2013-04-01 00:00:00

abstract：:The membrane-trafficking pathway mediated by tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) in neurons is still unknown. We show herein that TI-VAMP expression is necessary for neurite outgrowth in PC12 cells and hippocampal neurons in culture. TI-VAMP interacts with plasma membrane and e...

journal_title：Molecular biology of the cell

authors： Alberts P,Rudge R,Hinners I,Muzerelle A,Martinez-Arca S,Irinopoulou T,Marthiens V,Tooze S,Rathjen F,Gaspar P,Galli T

更新日期：2003-10-01 00:00:00

abstract：:HIF-1alpha is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1alpha protein is rapidly degraded in cells supplied with adequate oxygen but is stabilized in hypoxic cells. Using polysome profile analysis, we...

journal_title：Molecular biology of the cell

authors： Lang KJ,Kappel A,Goodall GJ

更新日期：2002-05-01 00:00:00

abstract：:Dynamics of endocytic clathrin-coated structures can be remarkably divergent across different cell types, cells within the same culture, or even distinct surfaces of the same cell. The origin of this astounding heterogeneity remains to be elucidated. Here we show that cellular processes associated with changes in effe...

journal_title：Molecular biology of the cell

authors： Willy NM,Ferguson JP,Huber SD,Heidotting SP,Aygün E,Wurm SA,Johnston-Halperin E,Poirier MG,Kural C

更新日期：2017-11-15 00:00:00

abstract：:The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

journal_title：Molecular biology of the cell

authors： Kalkan T,Iwasaki Y,Park CY,Thomsen GH

更新日期：2009-07-01 00:00:00

abstract：:In Schizosaccharomyces pombe, cytokinesis requires the assembly and constriction of an actomyosin-based contractile ring (CR). A single essential formin, Cdc12, localizes to the cell middle upon mitotic onset and nucleates the F-actin of the CR. Cdc12 medial recruitment is mediated in part by its direct binding to the...

journal_title：Molecular biology of the cell

authors： Willet AH,Bohnert KA,Gould KL

更新日期：2018-03-15 00:00:00

abstract：:Cell migration involves the cooperative reorganization of the actin and microtubule cytoskeletons, as well as the turnover of cell-substrate adhesions, under the control of Rho family GTPases. RhoA is activated at the leading edge of motile cells by unknown mechanisms to control actin stress fiber assembly, contractil...

journal_title：Molecular biology of the cell

authors： Nalbant P,Chang YC,Birkenfeld J,Chang ZF,Bokoch GM

更新日期：2009-09-01 00:00:00

abstract：:Axonemal dyneins are molecular motors that drive the beating of cilia and flagella. We report here the identification and partial cloning of seven unique axonemal dynein heavy chains from rat tracheal epithelial (RTE) cells. Combinations of axonemal-specific and degenerate primers to conserved regions around the catal...

journal_title：Molecular biology of the cell

authors： Andrews KL,Nettesheim P,Asai DJ,Ostrowski LE

更新日期：1996-01-01 00:00:00

abstract：:The human fungal pathogen Candida albicans can switch between yeast, pseudohyphal, and hyphal morphologies. To investigate whether the distinctive characteristics of hyphae are due to increased activity of the Cdc42 GTPase, strains lacking negative regulators of Cdc42 were constructed. Unexpectedly, the deletion of th...

journal_title：Molecular biology of the cell

authors： Court H,Sudbery P

更新日期：2007-01-01 00:00:00

abstract：:The endocytic and autophagic pathways are involved in the membrane trafficking of exogenous and endogenous materials to lysosomes. However, the mechanisms that regulate these pathways are largely unknown. We previously reported that Rubicon, a Beclin 1-binding protein, negatively regulates both the autophagic and endo...

journal_title：Molecular biology of the cell

authors： Tabata K,Matsunaga K,Sakane A,Sasaki T,Noda T,Yoshimori T

更新日期：2010-12-01 00:00:00

abstract：:Fusion of yeast vacuoles requires the Rab GTPase Ypt7p, four SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors), the SNARE disassembly chaperones Sec17p/Sec18p, vacuolar lipids, and the Rab-effector complex HOPS (homotypic fusion and vacuole protein sorting). Two HOPS subunits have direct ...

journal_title：Molecular biology of the cell

authors： Orr A,Wickner W,Rusin SF,Kettenbach AN,Zick M

更新日期：2015-01-15 00:00:00

abstract：:cAR1, a G protein-coupled receptor (GPCR) for cAMP, is required for the multicellular development of Dictyostelium. The activation of multiple pathways by cAR1 is transient because of poorly defined adaptation mechanisms. To investigate this, we used a genetic screen for impaired development to isolate four dominant-n...

journal_title：Molecular biology of the cell

authors： Zhang M,Goswami M,Hereld D

更新日期：2005-02-01 00:00:00

abstract：:A novel gene, prom-1, was isolated in a screen for Caenorhabditis elegans mutants with increased apoptosis in the germline. prom-1 encodes an F-box protein with limited homology to the putative human tumor suppressor FBXO47. Mutations in the prom-1 locus cause a strong reduction in bivalent formation, which results in...

journal_title：Molecular biology of the cell

authors： Jantsch V,Tang L,Pasierbek P,Penkner A,Nayak S,Baudrimont A,Schedl T,Gartner A,Loidl J

更新日期：2007-12-01 00:00:00

abstract：:A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal ...

journal_title：Molecular biology of the cell

authors： Hut HM,Lemstra W,Blaauw EH,Van Cappellen GW,Kampinga HH,Sibon OC

更新日期：2003-05-01 00:00:00

abstract：:In response to engagement of surface molecules, cells generate active forces that regulate many cellular processes. Developing tools that permit gathering mechanical and morphological information on these forces is of the utmost importance. Here we describe a new technique, the micropipette force probe, that uses a mi...

journal_title：Molecular biology of the cell

authors： Sawicka A,Babataheri A,Dogniaux S,Barakat AI,Gonzalez-Rodriguez D,Hivroz C,Husson J

更新日期：2017-11-07 00:00:00

abstract：:The process of macropinocytosis is an essential aspect of normal cell function, contributing to both growth and motile processes of cells. p21-activated kinases (PAKs) are targets for activated Rac and Cdc42 guanosine 5'-triphosphatases and have been shown to regulate the actin-myosin cytoskeleton. In fibroblasts PAK1...

journal_title：Molecular biology of the cell

authors： Dharmawardhane S,Schürmann A,Sells MA,Chernoff J,Schmid SL,Bokoch GM

更新日期：2000-10-01 00:00:00

abstract：:Murine connexin 40 (Cx40) and connexin 43 (Cx43) do not form functional heterotypic gap junction channels. This property may contribute to the preferential propagation of action potentials in murine conductive myocardium (expressing Cx40) which is surrounded by working myocardium, expressing Cx43. When mouse Cx40 and ...

journal_title：Molecular biology of the cell

authors： Haubrich S,Schwarz HJ,Bukauskas F,Lichtenberg-Fraté H,Traub O,Weingart R,Willecke K

更新日期：1996-12-01 00:00:00

abstract：:Two widely used hepatoma cell lines, mouse BW1J and human HepG2, express gene products characteristic of fetal hepatocytes, including serum albumin, whereas reporter genes driven by the albumin promoter are expressed at very low levels compared with highly differentiated hepatoma cells. We have investigated the low al...

journal_title：Molecular biology of the cell

authors： Rollier A,DiPersio CM,Cereghini S,Stevens K,Tronche F,Zaret K,Weiss MC

更新日期：1993-01-01 00:00:00

abstract：:An in vitro transport assay, established with a modified Shiga toxin B subunit (STxB) as a marker, has proved to be useful for the study of transport from the early/recycling endosome (EE/RE) to the trans-Golgi network (TGN). Here, we modified this assay to test antibodies to all known soluble N-ethylmaleimide-sensiti...

journal_title：Molecular biology of the cell

authors： Tai G,Lu L,Wang TL,Tang BL,Goud B,Johannes L,Hong W

更新日期：2004-09-01 00:00:00

abstract：:NSF and p97 are related AAA proteins implicated in membrane trafficking and organelle biogenesis. p97 is also involved in pathways that lead to ubiquitin-dependent proteolysis, including ER-associated degradation (ERAD). In this study, we have used dominant interfering ATP-hydrolysis deficient mutants (NSF(E329Q) and ...

journal_title：Molecular biology of the cell

authors： Dalal S,Rosser MF,Cyr DM,Hanson PI

更新日期：2004-02-01 00:00:00

abstract：:The demand for phenomics, a high-dimensional and high-throughput phenotyping method, has been increasing in many fields of biology. The budding yeast Saccharomyces cerevisiae, a unicellular model organism, provides an invaluable system for dissecting complex cellular processes using high-resolution phenotyping. Moreov...

journal_title：Molecular biology of the cell

authors： Ohya Y,Kimori Y,Okada H,Ohnuki S

更新日期：2015-11-05 00:00:00

abstract：:Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises from light excitation. An alternative is luciferase, an enzyme that ...

journal_title：Molecular biology of the cell

authors： Mazo-Vargas A,Park H,Aydin M,Buchler NE

更新日期：2014-11-05 00:00:00

abstract：:Despite the fact that the chromosomal passenger complex is well known to regulate kinetochore behavior in mitosis, no functional link has yet been established between the complex and kinetochore structure. In addition, remarkably little is known about how the complex targets to centromeres. Here, in a study of caspase...

journal_title：Molecular biology of the cell

authors： Faragher AJ,Sun XM,Butterworth M,Harper N,Mulheran M,Ruchaud S,Earnshaw WC,Cohen GM

更新日期：2007-04-01 00:00:00

abstract：:Yeast cells, when exposed to stress, can enter a protective state in which cell division, growth, and metabolism are down-regulated. They remain viable in this state until nutrients become available again. How cells enter this protective survival state and what happens at a cellular and subcellular level are largely u...

journal_title：Molecular biology of the cell

authors： Marini G,Nüske E,Leng W,Alberti S,Pigino G

更新日期：2020-06-01 00:00:00

abstract：:Cell emergence onto damaged or organized fibrous extracellular matrix (ECM) is a crucial precursor to collective cell migration in wound closure and cancer metastasis, respectively. However, there is a fundamental gap in our quantitative understanding of the role of local ECM size and arrangement in cell emergence-bas...

journal_title：Molecular biology of the cell

authors： Sharma P,Ng C,Jana A,Padhi A,Szymanski P,Lee JSH,Behkam B,Nain AS

更新日期：2017-09-15 00:00:00

abstract：:The tight junction adaptor protein ZO-1 regulates intracellular signaling and cell proliferation. Its Src homology 3 (SH3) domain is required for the regulation of proliferation and binds to the Y-box transcription factor ZO-1-associated nucleic acid binding protein (ZONAB). Binding of ZO-1 to ZONAB results in cytopla...

journal_title：Molecular biology of the cell

authors： Tsapara A,Matter K,Balda MS

更新日期：2006-03-01 00:00:00

abstract：:The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein heavy chain and may mediate the attachment of dynein to centrosomes and o...

journal_title：Molecular biology of the cell

authors： Mische S,He Y,Ma L,Li M,Serr M,Hays TS

更新日期：2008-11-01 00:00:00

abstract：:Rab8 is a GTPase involved in membrane trafficking. In photoreceptor cells, rab8 is proposed to participate in the late stages of delivery of rhodopsin-containing post-Golgi membranes to the plasma membrane near the base of the connecting cilium. To test the function of rab8 in vivo, we generated transgenic Xenopus lae...

journal_title：Molecular biology of the cell

authors： Moritz OL,Tam BM,Hurd LL,Peränen J,Deretic D,Papermaster DS

更新日期：2001-08-01 00:00:00