Mutant rab8 Impairs docking and fusion of rhodopsin-bearing post-Golgi membranes and causes cell death of transgenic Xenopus rods.

abstract：:Mitochondrial dysfunction is associated with the development of many age-related human diseases. Therefore recognizing and correcting the early signs of malfunctioning mitochondria is of critical importance for cellular welfare and survival. We previously demonstrated that VCP/Cdc48-associated mitochondrial stress res...

journal_title：Molecular biology of the cell

authors： Heo JM,Nielson JR,Dephoure N,Gygi SP,Rutter J

更新日期：2013-05-01 00:00:00

abstract：:Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated protein (MAP) kinases. Expression from the c-Fos, atrial natriuretic factor (ANF), and myosin light chain-2 (MLC-2) promoter...

journal_title：Molecular biology of the cell

authors： Thorburn J,Carlson M,Mansour SJ,Chien KR,Ahn NG,Thorburn A

更新日期：1995-11-01 00:00:00

abstract：:Large cargoes require multiple receptors for efficient transport through the nuclear pore complex. The 60S ribosomal subunit is one of the bulkiest transport cargoes, and in yeast three different receptors, Crm1, Mex67/Mtr2, and Arx1, collaborate in its export. However, only Crm1, recruited by the adapter Nmd3, appear...

journal_title：Molecular biology of the cell

authors： Lo KY,Johnson AW

更新日期：2009-03-01 00:00:00

abstract：:Mutations in the mitochondrial cardiolipin (CL) transacylase, tafazzin (Taz1p), result in the X-linked cardioskeletal myopathy, Barth syndrome (BTHS). The mitochondria of BTHS patients exhibit variable respiratory defects and abnormal cristae ultrastructure. The biochemical basis for these observations is unknown. In ...

journal_title：Molecular biology of the cell

authors： Claypool SM,Boontheung P,McCaffery JM,Loo JA,Koehler CM

更新日期：2008-12-01 00:00:00

abstract：:Rho GTPases share a common inhibitor, Rho guanine nucleotide dissociation inhibitor (RhoGDI), which regulates their expression levels, membrane localization, and activation state. The selective dissociation of individual Rho GTPases from RhoGDI ensures appropriate responses to cellular signals, but the underlying mech...

journal_title：Molecular biology of the cell

authors： Ard R,Mulatz K,Abramovici H,Maillet JC,Fottinger A,Foley T,Byham MR,Iqbal TA,Yoneda A,Couchman JR,Parks RJ,Gee SH

更新日期：2012-10-01 00:00:00

abstract：:Phosphoinositide 3-kinases (PI3K) are key molecular players in male fertility. However, the specific roles of different p110 PI3K catalytic subunits within the spermatogenic lineage have not been characterized so far. Herein, we report that male mice expressing a catalytically inactive p110beta develop testicular hypo...

journal_title：Molecular biology of the cell

authors： Ciraolo E,Morello F,Hobbs RM,Wolf F,Marone R,Iezzi M,Lu X,Mengozzi G,Altruda F,Sorba G,Guan K,Pandolfi PP,Wymann MP,Hirsch E

更新日期：2010-03-01 00:00:00

abstract：:Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

journal_title：Molecular biology of the cell

authors： Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

更新日期：2004-02-01 00:00:00

abstract：:Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

journal_title：Molecular biology of the cell

authors： Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

更新日期：2019-05-01 00:00:00

abstract：:Evidence has been presented both for and against obligate retrograde movement of resident Golgi proteins through the endoplasmic reticulum (ER) during nocodazole-induced Golgi ministack formation. Here, we studied the nocodazole-induced formation of ministacks using phospholipase A(2) (PLA(2)) antagonists, which have ...

journal_title：Molecular biology of the cell

authors： Drecktrah D,Brown WJ

更新日期：1999-12-01 00:00:00

abstract：:Motor neuron (MN) diseases are progressive disorders resulting from degeneration of neuromuscular junctions (NMJs), which form the connection between MNs and muscle fibers. NMJ-in-a-dish models have been developed to examine human MN-associated dysfunction with disease; however such coculture models have randomly orie...

journal_title：Molecular biology of the cell

authors： Happe CL,Tenerelli KP,Gromova AK,Kolb F,Engler AJ

更新日期：2017-07-07 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p lo...

journal_title：Molecular biology of the cell

authors： Pollard LW,Onishi M,Pringle JR,Lord M

更新日期：2012-07-01 00:00:00

abstract：:Cytochrome c release from mitochondria promotes apoptosome formation and caspase activation. The question as to whether mitochondrial permeabilization kills cells via a caspase-independent pathway when caspase activation is prevented is still open. Here we report that proneural cells of embryonic origin, when induced ...

journal_title：Molecular biology of the cell

authors： Ferraro E,Pulicati A,Cencioni MT,Cozzolino M,Navoni F,di Martino S,Nardacci R,Carrì MT,Cecconi F

更新日期：2008-08-01 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITPs) regulate the interface between lipid metabolism and specific steps in membrane trafficking through the secretory pathway in eukaryotes. Herein, we describe the cis-acting information that controls PITPbeta localization in mammalian cells. We demonstrate PITPbeta localizes...

journal_title：Molecular biology of the cell

authors： Phillips SE,Ile KE,Boukhelifa M,Huijbregts RP,Bankaitis VA

更新日期：2006-06-01 00:00:00

abstract：:Binding of echovirus 1 (EV1, a nonenveloped RNA virus) to the alpha2beta1 integrin on the cell surface is followed by endocytic internalization of the virus together with the receptor. Here, video-enhanced live microscopy revealed the rapid uptake of fluorescently labeled EV1 into mobile, intracellular structures, pos...

journal_title：Molecular biology of the cell

authors： Pietiäinen V,Marjomäki V,Upla P,Pelkmans L,Helenius A,Hyypiä T

更新日期：2004-11-01 00:00:00

abstract：:Golgi fragmentation is a common feature in multiple neurodegenerative diseases; however, the precise mechanism that causes fragmentation remains obscure. A potential link between Cdk5 and Golgi fragmentation in Alzheimer's disease (AD) was investigated in this study. Because Golgi is physiologically fragmented during ...

journal_title：Molecular biology of the cell

authors： Sun KH,de Pablo Y,Vincent F,Johnson EO,Chavers AK,Shah K

更新日期：2008-07-01 00:00:00

abstract：:To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants fused to synaptoph...

journal_title：Molecular biology of the cell

authors： Pennuto M,Dunlap D,Contestabile A,Benfenati F,Valtorta F

更新日期：2002-08-01 00:00:00

abstract：:We screened a human cDNA library with a probe derived from a partial SEC23 mouse homologue and isolated two different cDNA clones (hSec23A and hSec23B) encoding proteins of a predicted molecular mass of 85 kDa. hSec23Ap and hSec23Bp were 85% identical and shared 48% identity with the yeast Sec23p. Affinity-purified an...

journal_title：Molecular biology of the cell

authors： Paccaud JP,Reith W,Carpentier JL,Ravazzola M,Amherdt M,Schekman R,Orci L

更新日期：1996-10-01 00:00:00

abstract：:Op18/stathmin (Op18) is a phosphorylation-regulated microtubule destabilizer that is frequently overexpressed in tumors. The importance of Op18 in malignancy was recently suggested by identification of a somatic Q18-->E mutation of Op18 in an adenocarcinoma. We addressed the functional consequences of aberrant Op18 ex...

journal_title：Molecular biology of the cell

authors： Holmfeldt P,Brännström K,Stenmark S,Gullberg M

更新日期：2006-07-01 00:00:00

abstract：:The second step of dolichol-linked oligosaccharide synthesis in the N-linked glycosylation pathway at the endoplasmic reticulum (ER) membrane is catalyzed by an unusual hetero-oligomeric UDP-N-acetylglucosamine transferase that in most eukaryotes is comprised of at least two subunits, Alg13p and Alg14p. Alg13p is the ...

journal_title：Molecular biology of the cell

authors： Averbeck N,Gao XD,Nishimura S,Dean N

更新日期：2008-05-01 00:00:00

abstract：:Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) at the inner leaflet of the plasma membrane has been proposed to locally regulate the actin cytoskeleton. Indeed, recent studies that use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent PIP(2) sensors suggest that this lipid is enriched in membrane microdo...

journal_title：Molecular biology of the cell

authors： van Rheenen J,Jalink K

更新日期：2002-09-01 00:00:00

abstract：:Clathrin-mediated endocytosis regulates the internalization of many nutrient and signaling receptors. Clathrin and endocytic accessory proteins are recruited to receptors by specific adaptors. The adaptor Disabled-2 (Dab2) recruits its cargoes, including the low-density lipoprotein receptor (LDLR), and mediates endocy...

journal_title：Molecular biology of the cell

authors： Mulkearns EE,Cooper JA

更新日期：2012-04-01 00:00:00

abstract：:Calreticulin (CRT) is a high-capacity, low-affinity Ca2+-binding protein located in the lumen of the endoplasmic reticulum (ER) of all eukaryotic cells investigated so far. Its high level of conservation among different species suggests that it serves functions fundamental to cell survival. The role originally propose...

journal_title：Molecular biology of the cell

authors： Fasolato C,Pizzo P,Pozzan T

更新日期：1998-06-01 00:00:00

abstract：:We have used E1A probes to study the roles of the p34cdc2 kinase and the retinoblastoma tumor susceptibility gene product (pRB) in transforming growth factor beta 1 (TGF beta 1)-mediated growth suppression in mink lung epithelial (Mv1Lu) cells. In agreement with previous reports, we see a decline in p34cdc2 kinase act...

journal_title：Molecular biology of the cell

authors： Abraham SE,Carter MC,Moran E

更新日期：1992-06-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00

abstract：:In biogenesis of membrane proteins on the endoplasmic reticulum, a protein-conducting channel called the translocon functions in both the membrane translocation of lumenal domains and the integration of transmembrane segments. Here we analyzed the environments of polypeptide chains during the processes by water-depend...

journal_title：Molecular biology of the cell

authors： Kida Y,Kume C,Hirano M,Sakaguchi M

更新日期：2010-02-01 00:00:00

abstract：:The nuclear lamina is a key structural element of the metazoan nucleus. However, the structural organization of the major proteins composing the lamina is poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized the supramolecular structures of lamin...

journal_title：Molecular biology of the cell

authors： Shimi T,Kittisopikul M,Tran J,Goldman AE,Adam SA,Zheng Y,Jaqaman K,Goldman RD

更新日期：2015-11-05 00:00:00

abstract：:We recently determined the nucleotide sequence of the gene encoding p28, a light chain of inner dynein arms of Chlamydomonas axonemes. Here, we show that p28 is the protein encoded by the IDA4 locus. p28, and the dynein heavy chains normally associated with it, are completely absent from the flagella and cell bodies o...

journal_title：Molecular biology of the cell

authors： LeDizet M,Piperno G

更新日期：1995-06-01 00:00:00

abstract：:Myosin II-dependent contraction of the contractile ring drives equatorial furrowing during cytokinesis in animal cells. Nonetheless, myosin II-null cells of the cellular slime mold Dictyostelium divide efficiently when adhering to substrates by making use of polar traction forces. Here, we show that in the presence of...

journal_title：Molecular biology of the cell

authors： Kanada M,Nagasaki A,Uyeda TQ

更新日期：2005-08-01 00:00:00

abstract：:Shiga-toxin-producing Escherichia coli remain a food-borne health threat. Shiga toxin is endocytosed by intestinal epithelial cells and transported retrogradely through the secretory pathway. It is ultimately translocated to the cytosol where it inhibits protein translation. We found that Shiga toxin transport through...

journal_title：Molecular biology of the cell

authors： Hehnly H,Longhini KM,Chen JL,Stamnes M

更新日期：2009-10-01 00:00:00