Hypoxia-inducible factor-1alpha mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia.


:HIF-1alpha is the regulated subunit of the HIF-1 transcription factor, which induces transcription of a number of genes involved in the cellular response to hypoxia. The HIF-1alpha protein is rapidly degraded in cells supplied with adequate oxygen but is stabilized in hypoxic cells. Using polysome profile analysis, we found that translation of HIF-1alpha mRNA in NIH3T3 cells is spared the general reduction in translation rate that occurs during hypoxia. To assess whether the 5'UTR of the HIF-1alpha mRNA contains an internal ribosome entry site (IRES), we constructed a dicistronic reporter with the HIF-1alpha 5'UTR inserted between two reporter coding regions. We found that the HIF-1alpha 5'UTR promoted translation of the downstream reporter, indicating the presence of an IRES. The IRES had activity comparable to that of the well-characterized c-myc IRES. IRES activity was not affected by hypoxic conditions that caused a reduction in cap-dependent translation, and IRES activity was less affected by serum-starvation than was cap-dependent translation. These data indicate that the presence of an IRES in the HIF-1alpha 5'UTR allows translation to be maintained under conditions that are inhibitory to cap-dependent translation.


Mol Biol Cell


Lang KJ,Kappel A,Goodall GJ





Has Abstract


2002-05-01 00:00:00












  • Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking but not silencing of endocytosed receptors.

    abstract::The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase Fab1/PIKfyve controls trafficking but not silencing of endocytosed receptors. Drosophila fab1 mutants contain undetecta...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Rusten TE,Rodahl LM,Pattni K,Englund C,Samakovlis C,Dove S,Brech A,Stenmark H

    更新日期:2006-09-01 00:00:00

  • Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway.

    abstract::The sorting of most integral membrane proteins into the lumenal vesicles of multivesicular bodies (MVBs) is dependent on the attachment of ubiquitin (Ub) to their cytosolic domains. However, Ub is not required for sorting of Sna3, an MVB vesicle cargo protein in yeast. We show that Sna3 circumvents Ub-mediated recogni...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: McNatt MW,McKittrick I,West M,Odorizzi G

    更新日期:2007-02-01 00:00:00

  • Yeast actin cytoskeleton mutants accumulate a new class of Golgi-derived secretary vesicle.

    abstract::Many yeast actin cytoskeleton mutants accumulate large secretory vesicles and exhibit phenotypes consistent with defects in polarized growth. This, together with actin's polarized organization, has suggested a role for the actin cytoskeleton in the vectorial transport of late secretory vesicles to the plasma membrane....

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Mulholland J,Wesp A,Riezman H,Botstein D

    更新日期:1997-08-01 00:00:00

  • Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex.

    abstract::Targeted endocytosis of plasma membrane (PM) proteins allows cells to adjust their complement of membrane proteins to changing extracellular conditions. For a wide variety of PM proteins, initiation of endocytosis is triggered by ubiquitination. In yeast, arrestin-related trafficking adaptors (ARTs) enable a single ub...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Guiney EL,Klecker T,Emr SD

    更新日期:2016-12-15 00:00:00

  • Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism.

    abstract::Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin-dependent cell...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Mary S,Charrasse S,Meriane M,Comunale F,Travo P,Blangy A,Gauthier-Rouvière C

    更新日期:2002-01-01 00:00:00

  • Mechanistic insights into actin-driven polarity site movement in yeast.

    abstract::Directed cell growth or migration are critical for the development and function of many eukaryotic cells. These cells develop a dynamic "front" (also called "polarity site") that can change direction. Polarity establishment involves autocatalytic accumulation of polarity regulators, including the conserved Rho-family ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ghose D,Lew D

    更新日期:2020-05-01 00:00:00

  • Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy.

    abstract::In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuol...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kabeya Y,Kamada Y,Baba M,Takikawa H,Sasaki M,Ohsumi Y

    更新日期:2005-05-01 00:00:00

  • An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation.

    abstract::The TOR kinases are conserved negative regulators of autophagy in response to nutrient conditions, but the signaling mechanisms are poorly understood. Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila. We s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chang YY,Neufeld TP

    更新日期:2009-04-01 00:00:00

  • Activation and functional analysis of Janus kinase 2 in BA/F3 cells using the coumermycin/gyrase B system.

    abstract::Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Mohi MG,Arai Ki,Watanabe S

    更新日期:1998-12-01 00:00:00

  • Roles of NUDE and NUDF proteins of Aspergillus nidulans: insights from intracellular localization and overexpression effects.

    abstract::The NUDF protein of the filamentous fungus Aspergillus nidulans functions in the cytoplasmic dynein pathway. It binds several proteins, including the NUDE protein. Green fluorescent protein-tagged NUDF and NUDA (dynein heavy chain) localize to linearly moving dashes ("comets") that coincide with microtubule ends. Here...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Efimov VP

    更新日期:2003-03-01 00:00:00

  • Self-oligomerization regulates stability of survival motor neuron protein isoforms by sequestering an SCFSlmb degron.

    abstract::Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1 Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMNΔ7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Gray KM,Kaifer KA,Baillat D,Wen Y,Bonacci TR,Ebert AD,Raimer AC,Spring AM,Have ST,Glascock JJ,Gupta K,Van Duyne GD,Emanuele MJ,Lamond AI,Wagner EJ,Lorson CL,Matera AG

    更新日期:2018-01-15 00:00:00

  • Calponin 3 regulates actin cytoskeleton rearrangement in trophoblastic cell fusion.

    abstract::Cell-cell fusion is an intriguing differentiation process, essential for placental development and maturation. A proteomic approach identified a cytoplasmic protein, calponin 3 (CNN3), related to the fusion of BeWo choriocarcinoma cells. CNN3 was expressed in cytotrophoblasts in human placenta. CNN3 gene knockdown pro...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Shibukawa Y,Yamazaki N,Kumasawa K,Daimon E,Tajiri M,Okada Y,Ikawa M,Wada Y

    更新日期:2010-11-15 00:00:00

  • Deletion of nudC, a nuclear migration gene of Aspergillus nidulans, causes morphological and cell wall abnormalities and is lethal.

    abstract::Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Chiu YH,Xiang X,Dawe AL,Morris NR

    更新日期:1997-09-01 00:00:00

  • Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres.

    abstract::Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR) resides in the nucleo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Jády BE,Richard P,Bertrand E,Kiss T

    更新日期:2006-02-01 00:00:00

  • Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes.

    abstract::Dysregulated hepatic cholesterol homeostasis with free cholesterol accumulation in the liver is relevant to the pathogenesis of nonalcoholic steatohepatitis, contributing to the chronicity of liver toxicity. Here we examined the effect of free cholesterol accumulation on the morphology and biochemical properties of li...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Makino A,Hullin-Matsuda F,Murate M,Abe M,Tomishige N,Fukuda M,Yamashita S,Fujimoto T,Vidal H,Lagarde M,Delton I,Kobayashi T

    更新日期:2016-11-01 00:00:00

  • CD1 and major histocompatibility complex II molecules follow a different course during dendritic cell maturation.

    abstract::The maturation of dendritic cells is accompanied by the redistribution of major histocompatibility complex (MHC) class II molecules from the lysosomal MHC class II compartment to the plasma membrane to mediate presentation of peptide antigens. Besides MHC molecules, dendritic cells also express CD1 molecules that medi...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: van der Wel NN,Sugita M,Fluitsma DM,Cao X,Schreibelt G,Brenner MB,Peters PJ

    更新日期:2003-08-01 00:00:00

  • Profilin connects actin assembly with microtubule dynamics.

    abstract::Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization f...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Nejedla M,Sadi S,Sulimenko V,de Almeida FN,Blom H,Draber P,Aspenström P,Karlsson R

    更新日期:2016-08-01 00:00:00

  • pp60v-src transformation of rat cells but not chicken cells strongly correlates with low-affinity phosphopeptide binding by the SH2 domain.

    abstract::Substrates critical for transformation by pp60v-src remain unknown, as does the precise role of the src homology 2 (SH2) domain in this process. To continue exploring the role of the SH2 domain in pp60v-src-mediated transformation, site-directed mutagenesis was used to create mutant v-src alleles predicted to encode p...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Verderame MF

    更新日期:1997-05-01 00:00:00

  • Fission yeast Ste9, a homolog of Hct1/Cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase.

    abstract::When proliferating fission yeast cells are exposed to nitrogen starvation, they initiate conjugation and differentiate into ascospores. Cell cycle arrest in the G1-phase is one of the prerequisites for cell differentiation, because conjugation occurs only in the pre-Start G1-phase. The role of ste9(+) in the cell cycl...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kitamura K,Maekawa H,Shimoda C

    更新日期:1998-05-01 00:00:00

  • Dissection of the NUP107 nuclear pore subcomplex reveals a novel interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis elegans.

    abstract::Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a gen...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Ródenas E,González-Aguilera C,Ayuso C,Askjaer P

    更新日期:2012-03-01 00:00:00

  • Septins, under Cla4p regulation, and the chitin ring are required for neck integrity in budding yeast.

    abstract::CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 m...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Schmidt M,Varma A,Drgon T,Bowers B,Cabib E

    更新日期:2003-05-01 00:00:00

  • Developmental expression of spectrins in rat skeletal muscle.

    abstract::Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Zhou D,Ursitti JA,Bloch RJ

    更新日期:1998-01-01 00:00:00

  • Inhibition of a signaling pathway in cardiac muscle cells by active mitogen-activated protein kinase kinase.

    abstract::Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated protein (MAP) kinases. Expression from the c-Fos, atrial natriuretic factor (ANF), and myosin light chain-2 (MLC-2) promoter...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Thorburn J,Carlson M,Mansour SJ,Chien KR,Ahn NG,Thorburn A

    更新日期:1995-11-01 00:00:00

  • Inositol 1,4,5-trisphosphate receptors in endocrine cells: localization and association in hetero- and homotetramers.

    abstract::The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular calcium channel involved in coupling cell membrane receptors to calcium signal transduction pathways within cells including endocrine cells. Several isoforms (I, II, and III) of IP3Rs have been identified, which are encoded by separate genes, and are...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Nucifora FC Jr,Sharp AH,Milgram SL,Ross CA

    更新日期:1996-06-01 00:00:00

  • Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.

    abstract::The cdc25 tyrosine phosphatase is known to activate cdc2 kinase in the G2/M transition by dephosphorylation of tyrosine 15. To determine how entry into M-phase in eukaryotic cells is controlled, we have investigated the regulation of the cdc25 protein in Xenopus eggs and oocytes. Two closely related Xenopus cdc25 gene...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Izumi T,Walker DH,Maller JL

    更新日期:1992-08-01 00:00:00

  • Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation.

    abstract::The Schizosaccharomyces pombe sts1+ gene, identified by supersensitive mutations to a protein kinase inhibitor, staurosporine, was isolated by complementation by the use of a fission yeast genomic library. Nucleotide sequencing shows that the sts1+ gene encodes a 453 amino acid putative membrane-associated protein tha...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Shimanuki M,Goebl M,Yanagida M,Toda T

    更新日期:1992-03-01 00:00:00

  • Interaction between a Ras and a Rho GTPase couples selection of a growth site to the development of cell polarity in yeast.

    abstract::Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

    更新日期:2003-12-01 00:00:00

  • A synthetic peptide from the COOH-terminal heparin-binding domain of fibronectin promotes focal adhesion formation.

    abstract::Cell adhesion to extracellular matrix molecules such as fibronectin involves complex transmembrane signaling processes. Attachment and spreading of primary fibroblasts can be promoted by interactions of cell surface integrins with RGD-containing fragments of fibronectin, but the further process of focal adhesion and s...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Woods A,McCarthy JB,Furcht LT,Couchman JR

    更新日期:1993-06-01 00:00:00

  • Tumor necrosis factor-receptor-associated factor-4 is a positive regulator of transforming growth factor-beta signaling that affects neural crest formation.

    abstract::The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Kalkan T,Iwasaki Y,Park CY,Thomsen GH

    更新日期:2009-07-01 00:00:00

  • Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S. cerevisiae.

    abstract::Inactivation of YME1 in yeast causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment. T...

    journal_title:Molecular biology of the cell

    pub_type: 杂志文章


    authors: Weber ER,Hanekamp T,Thorsness PE

    更新日期:1996-02-01 00:00:00