Hypoxia-inducible factor-1alpha mRNA contains an internal ribosome entry site that allows efficient translation during normoxia and hypoxia.

abstract：:The trafficking of endocytosed receptors through phosphatidylinositol 3-phosphate [PtdIns(3)P]-containing endosomes is thought to attenuate their signaling. Here, we show that the PtdIns(3)P 5-kinase Fab1/PIKfyve controls trafficking but not silencing of endocytosed receptors. Drosophila fab1 mutants contain undetecta...

journal_title：Molecular biology of the cell

authors： Rusten TE,Rodahl LM,Pattni K,Englund C,Samakovlis C,Dove S,Brech A,Stenmark H

更新日期：2006-09-01 00:00:00

abstract：:The sorting of most integral membrane proteins into the lumenal vesicles of multivesicular bodies (MVBs) is dependent on the attachment of ubiquitin (Ub) to their cytosolic domains. However, Ub is not required for sorting of Sna3, an MVB vesicle cargo protein in yeast. We show that Sna3 circumvents Ub-mediated recogni...

journal_title：Molecular biology of the cell

authors： McNatt MW,McKittrick I,West M,Odorizzi G

更新日期：2007-02-01 00:00:00

abstract：:Many yeast actin cytoskeleton mutants accumulate large secretory vesicles and exhibit phenotypes consistent with defects in polarized growth. This, together with actin's polarized organization, has suggested a role for the actin cytoskeleton in the vectorial transport of late secretory vesicles to the plasma membrane....

journal_title：Molecular biology of the cell

authors： Mulholland J,Wesp A,Riezman H,Botstein D

更新日期：1997-08-01 00:00:00

abstract：:Targeted endocytosis of plasma membrane (PM) proteins allows cells to adjust their complement of membrane proteins to changing extracellular conditions. For a wide variety of PM proteins, initiation of endocytosis is triggered by ubiquitination. In yeast, arrestin-related trafficking adaptors (ARTs) enable a single ub...

journal_title：Molecular biology of the cell

authors： Guiney EL,Klecker T,Emr SD

更新日期：2016-12-15 00:00:00

abstract：:Cadherin-mediated cell-cell adhesion is a dynamic process that is regulated during embryonic development, cell migration, and differentiation. Different cadherins are expressed in specific tissues consistent with their roles in cell type recognition. In this study, we examine the formation of N-cadherin-dependent cell...

journal_title：Molecular biology of the cell

authors： Mary S,Charrasse S,Meriane M,Comunale F,Travo P,Blangy A,Gauthier-Rouvière C

更新日期：2002-01-01 00:00:00

abstract：:Directed cell growth or migration are critical for the development and function of many eukaryotic cells. These cells develop a dynamic "front" (also called "polarity site") that can change direction. Polarity establishment involves autocatalytic accumulation of polarity regulators, including the conserved Rho-family ...

journal_title：Molecular biology of the cell

authors： Ghose D,Lew D

更新日期：2020-05-01 00:00:00

abstract：:In eukaryotic cells, nutrient starvation induces the bulk degradation of cellular materials; this process is called autophagy. In the yeast Saccharomyces cerevisiae, most of the ATG (autophagy) genes are involved in not only the process of degradative autophagy, but also a biosynthetic process, the cytoplasm to vacuol...

journal_title：Molecular biology of the cell

authors： Kabeya Y,Kamada Y,Baba M,Takikawa H,Sasaki M,Ohsumi Y

更新日期：2005-05-01 00:00:00

abstract：:The TOR kinases are conserved negative regulators of autophagy in response to nutrient conditions, but the signaling mechanisms are poorly understood. Here we describe a complex containing the protein kinase Atg1 and the phosphoprotein Atg13 that functions as a critical component of this regulation in Drosophila. We s...

journal_title：Molecular biology of the cell

authors： Chang YY,Neufeld TP

更新日期：2009-04-01 00:00:00

abstract：:Janus kinase 2 (Jak2) protein tyrosine kinase plays an important role in interleukin-3- or granulocyte-macrophage colony-stimulating factor-mediated signal transduction pathways leading to cell proliferation, activation of early response genes, and inhibition of apoptosis. However, it is unclear whether Jak2 can activ...

journal_title：Molecular biology of the cell

authors： Mohi MG,Arai Ki,Watanabe S

更新日期：1998-12-01 00:00:00

abstract：:The NUDF protein of the filamentous fungus Aspergillus nidulans functions in the cytoplasmic dynein pathway. It binds several proteins, including the NUDE protein. Green fluorescent protein-tagged NUDF and NUDA (dynein heavy chain) localize to linearly moving dashes ("comets") that coincide with microtubule ends. Here...

journal_title：Molecular biology of the cell

authors： Efimov VP

更新日期：2003-03-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is caused by homozygous mutations in human SMN1 Expression of a duplicate gene (SMN2) primarily results in skipping of exon 7 and production of an unstable protein isoform, SMNΔ7. Although SMN2 exon skipping is the principal contributor to SMA severity, mechanisms governing stability of s...

journal_title：Molecular biology of the cell

authors： Gray KM,Kaifer KA,Baillat D,Wen Y,Bonacci TR,Ebert AD,Raimer AC,Spring AM,Have ST,Glascock JJ,Gupta K,Van Duyne GD,Emanuele MJ,Lamond AI,Wagner EJ,Lorson CL,Matera AG

更新日期：2018-01-15 00:00:00

abstract：:Cell-cell fusion is an intriguing differentiation process, essential for placental development and maturation. A proteomic approach identified a cytoplasmic protein, calponin 3 (CNN3), related to the fusion of BeWo choriocarcinoma cells. CNN3 was expressed in cytotrophoblasts in human placenta. CNN3 gene knockdown pro...

journal_title：Molecular biology of the cell

authors： Shibukawa Y,Yamazaki N,Kumasawa K,Daimon E,Tajiri M,Okada Y,Ikawa M,Wada Y

更新日期：2010-11-15 00:00:00

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR) resides in the nucleo...

journal_title：Molecular biology of the cell

authors： Jády BE,Richard P,Bertrand E,Kiss T

更新日期：2006-02-01 00:00:00

abstract：:Dysregulated hepatic cholesterol homeostasis with free cholesterol accumulation in the liver is relevant to the pathogenesis of nonalcoholic steatohepatitis, contributing to the chronicity of liver toxicity. Here we examined the effect of free cholesterol accumulation on the morphology and biochemical properties of li...

journal_title：Molecular biology of the cell

authors： Makino A,Hullin-Matsuda F,Murate M,Abe M,Tomishige N,Fukuda M,Yamashita S,Fujimoto T,Vidal H,Lagarde M,Delton I,Kobayashi T

更新日期：2016-11-01 00:00:00

abstract：:The maturation of dendritic cells is accompanied by the redistribution of major histocompatibility complex (MHC) class II molecules from the lysosomal MHC class II compartment to the plasma membrane to mediate presentation of peptide antigens. Besides MHC molecules, dendritic cells also express CD1 molecules that medi...

journal_title：Molecular biology of the cell

authors： van der Wel NN,Sugita M,Fluitsma DM,Cao X,Schreibelt G,Brenner MB,Peters PJ

更新日期：2003-08-01 00:00:00

abstract：:Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization f...

journal_title：Molecular biology of the cell

authors： Nejedla M,Sadi S,Sulimenko V,de Almeida FN,Blom H,Draber P,Aspenström P,Karlsson R

更新日期：2016-08-01 00:00:00

abstract：:Substrates critical for transformation by pp60v-src remain unknown, as does the precise role of the src homology 2 (SH2) domain in this process. To continue exploring the role of the SH2 domain in pp60v-src-mediated transformation, site-directed mutagenesis was used to create mutant v-src alleles predicted to encode p...

journal_title：Molecular biology of the cell

authors： Verderame MF

更新日期：1997-05-01 00:00:00

abstract：:When proliferating fission yeast cells are exposed to nitrogen starvation, they initiate conjugation and differentiate into ascospores. Cell cycle arrest in the G1-phase is one of the prerequisites for cell differentiation, because conjugation occurs only in the pre-Start G1-phase. The role of ste9(+) in the cell cycl...

journal_title：Molecular biology of the cell

authors： Kitamura K,Maekawa H,Shimoda C

更新日期：1998-05-01 00:00:00

abstract：:Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a gen...

journal_title：Molecular biology of the cell

authors： Ródenas E,González-Aguilera C,Ayuso C,Askjaer P

更新日期：2012-03-01 00:00:00

abstract：:CLA4, encoding a protein kinase of the PAK type, and CDC11, encoding a septin, were isolated in a screen for synthetic lethality with CHS3, which encodes the chitin synthase III catalytic moiety. Although Ste20p shares some essential function with Cla4p, it did not show synthetic lethality with Chs3p. cla4 and cdc11 m...

journal_title：Molecular biology of the cell

authors： Schmidt M,Varma A,Drgon T,Bowers B,Cabib E

更新日期：2003-05-01 00:00:00

abstract：:Skeletal muscle contains spectrin (or spectrin I) and fodrin (or spectrin II), members of the spectrin supergene family. We used isoform-specific antibodies and cDNA probes to investigate the molecular forms, developmental expression, and subcellular localization of the spectrins in skeletal muscle of the rat. We repo...

journal_title：Molecular biology of the cell

authors： Zhou D,Ursitti JA,Bloch RJ

更新日期：1998-01-01 00:00:00

abstract：:Signaling via the Ras pathway involves sequential activation of Ras, Raf-1, mitogen-activated protein kinase kinase (MKK), and the extracellular signal-regulated (ERK) group of mitogen-activated protein (MAP) kinases. Expression from the c-Fos, atrial natriuretic factor (ANF), and myosin light chain-2 (MLC-2) promoter...

journal_title：Molecular biology of the cell

authors： Thorburn J,Carlson M,Mansour SJ,Chien KR,Ahn NG,Thorburn A

更新日期：1995-11-01 00:00:00

abstract：:The inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular calcium channel involved in coupling cell membrane receptors to calcium signal transduction pathways within cells including endocrine cells. Several isoforms (I, II, and III) of IP3Rs have been identified, which are encoded by separate genes, and are...

journal_title：Molecular biology of the cell

authors： Nucifora FC Jr,Sharp AH,Milgram SL,Ross CA

更新日期：1996-06-01 00:00:00

abstract：:The cdc25 tyrosine phosphatase is known to activate cdc2 kinase in the G2/M transition by dephosphorylation of tyrosine 15. To determine how entry into M-phase in eukaryotic cells is controlled, we have investigated the regulation of the cdc25 protein in Xenopus eggs and oocytes. Two closely related Xenopus cdc25 gene...

journal_title：Molecular biology of the cell

authors： Izumi T,Walker DH,Maller JL

更新日期：1992-08-01 00:00:00

abstract：:The Schizosaccharomyces pombe sts1+ gene, identified by supersensitive mutations to a protein kinase inhibitor, staurosporine, was isolated by complementation by the use of a fission yeast genomic library. Nucleotide sequencing shows that the sts1+ gene encodes a 453 amino acid putative membrane-associated protein tha...

journal_title：Molecular biology of the cell

authors： Shimanuki M,Goebl M,Yanagida M,Toda T

更新日期：1992-03-01 00:00:00

abstract：:Polarized cell growth requires the coupling of a defined spatial site on the cell cortex to the apparatus that directs the establishment of cell polarity. In the budding yeast Saccharomyces cerevisiae, the Ras-family GTPase Rsr1p/Bud1p and its regulators select the proper site for bud emergence on the cell cortex. The...

journal_title：Molecular biology of the cell

authors： Kozminski KG,Beven L,Angerman E,Tong AH,Boone C,Park HO

更新日期：2003-12-01 00:00:00

abstract：:Cell adhesion to extracellular matrix molecules such as fibronectin involves complex transmembrane signaling processes. Attachment and spreading of primary fibroblasts can be promoted by interactions of cell surface integrins with RGD-containing fragments of fibronectin, but the further process of focal adhesion and s...

journal_title：Molecular biology of the cell

authors： Woods A,McCarthy JB,Furcht LT,Couchman JR

更新日期：1993-06-01 00:00:00

abstract：:The transforming growth factor (TGF)-beta superfamily regulates cell proliferation, apoptosis, differentiation, migration, and development. Canonical TGFbeta signals are transduced to the nucleus via Smads in both major signaling branches, bone morphogenetic protein (BMP) or Activin/Nodal/TGFbeta. Smurf ubiquitin (Ub)...

journal_title：Molecular biology of the cell

authors： Kalkan T,Iwasaki Y,Park CY,Thomsen GH

更新日期：2009-07-01 00:00:00

abstract：:Inactivation of YME1 in yeast causes several distinct phenotypes: an increased rate of DNA escape from mitochondria, temperature-sensitive growth on nonfermentable carbon sources, extremely slow growth when mitochondrial DNA is completely absent from the cell, and altered morphology of the mitochondrial compartment. T...

journal_title：Molecular biology of the cell

authors： Weber ER,Hanekamp T,Thorsness PE

更新日期：1996-02-01 00:00:00