Abstract:
:In most eukaryotes, genes encoding ribosomal RNAs (rDNA) are clustered in long tandem head-to-tail repeats. Studies of Saccharomyces cerevisiae have indicated that rDNA copy number is maintained through recombination events associated with site-specific blockage of replication forks (RFs). Here, we describe two Schizosaccharomyces pombe proteins, homologs of S. cerevisiae Slx1 and Slx4, as subunits of a novel type of endonuclease that maintains rDNA copy number. The Slx1-Slx4-dependent endonuclease introduces single-strand cuts in duplex DNA on the 3' side of junctions with single-strand DNA. Deletion of Slx1 or Rqh1 RecQ-like DNA helicase provokes rDNA contraction, whereas simultaneous elimination of Slx1-Slx4 endonuclease and Rqh1 is lethal. Slx1 associates with chromatin at two foci characteristic of the two rDNA repeat loci in S. pombe. We propose a model in which the Slx1-Slx4 complex is involved in the control of the expansion and contraction of the rDNA loci by initiating recombination events at stalled RFs.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Coulon S,Gaillard PHL,Chahwan C,McDonald WH,Yates JR 3rd,Russell Pdoi
10.1091/mbc.e03-08-0586keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
71-80issue
1eissn
1059-1524issn
1939-4586pii
E03-08-0586journal_volume
15pub_type
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