Molecular basis for phosphospecific recognition of histone H3 tails by Survivin paralogues at inner centromeres.

abstract：:Drosophila bristles display a precise orientation and curvature. An asymmetric extension of the socket cell overlies the newly emerging bristle rudiment to provide direction for bristle elongation, a process thought to be orchestrated by the nerve dendrite lying between these cells. Scanning electron microscopic analy...

journal_title：Molecular biology of the cell

authors： Tilney LG,Connelly PS,Ruggiero L,Vranich KA,Guild GM,Derosier D

更新日期：2004-12-01 00:00:00

abstract：:Forces generated by heart muscle contraction must be balanced by adhesion to the extracellular matrix (ECM) and to other cells for proper heart function. Decades of data have suggested that cell-ECM adhesions are important for sarcomere assembly. However, the relationship between cell-ECM adhesions and sarcomeres asse...

journal_title：Molecular biology of the cell

authors： Taneja N,Neininger AC,Burnette DT

更新日期：2020-06-01 00:00:00

abstract：:Preventing polyspermy during animal fertilization relies on modifications to the egg's extracellular matrix. On fertilization in sea urchins, the contents of cortical granules are secreted and rapidly assemble into the egg's extracellular vitelline layer, forming the fertilization envelope, a proteinaceous structure t...

journal_title：Molecular biology of the cell

authors： Wong JL,Wessel GM

更新日期：2006-12-01 00:00:00

abstract：:Formation and discharge of dense-core secretory vesicles depend on controlled rearrangement of the core proteins during their assembly and dispersal. The ciliate Tetrahymena thermophila offers a simple system in which the mechanisms may be studied. Here we show that most of the core consists of a set of polypeptides d...

journal_title：Molecular biology of the cell

authors： Verbsky JW,Turkewitz AP

更新日期：1998-02-01 00:00:00

abstract：:The putative role of sorting early endosomes (EEs) in synaptic-like microvesicle (SLMV) formation in the neuroendocrine PC12 cell line was investigated by quantitative immunoelectron microscopy. By BSA-gold internalization kinetics, four distinct endosomal subcompartments were distinguished: primary endocytic vesicles...

journal_title：Molecular biology of the cell

authors： de Wit H,Lichtenstein Y,Geuze HJ,Kelly RB,van der Sluijs P,Klumperman J

更新日期：1999-12-01 00:00:00

abstract：:In vitro Ca++ activates gelsolin to sever F-actin and form a gelsolin-actin (GA) complex at the+end of F-actin that is not dissociated by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) but is separated by EGTA+PIP/PIP2. The gelsolin blocks the+end on the actin filament, but the-end of the...

journal_title：Molecular biology of the cell

authors： Deaton JD,Guerrero T,Howard TH

更新日期：1992-12-01 00:00:00

abstract：:Formation of multiple-protein macromolecular complexes at specialized subcellular microdomains increases the specificity and efficiency of signaling in cells. In this study, we demonstrate that phosphodiesterase type 3A (PDE3A) physically and functionally interacts with cystic fibrosis transmembrane conductance regula...

journal_title：Molecular biology of the cell

authors： Penmatsa H,Zhang W,Yarlagadda S,Li C,Conoley VG,Yue J,Bahouth SW,Buddington RK,Zhang G,Nelson DJ,Sonecha MD,Manganiello V,Wine JJ,Naren AP

更新日期：2010-03-15 00:00:00

abstract：:Conformational diseases are associated with the conversion of normal proteins into aggregation-prone toxic conformers with structures similar to that of β-amyloid. Spatial distribution of amyloid-like proteins into intracellular quality control centers can be beneficial, but cellular mechanisms for protective aggregat...

journal_title：Molecular biology of the cell

authors： Wolfe KJ,Ren HY,Trepte P,Cyr DM

更新日期：2013-12-01 00:00:00

abstract：:Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell-cell contacts. We investigated the changes in focal adhesion proteins during etoposide-induced apoptosis in Rat-1 cells and found that during apoptosis, p130cas (...

journal_title：Molecular biology of the cell

authors： Kook S,Shim SR,Choi SJ,Ahnn J,Kim JI,Eom SH,Jung YK,Paik SG,Song WK

更新日期：2000-03-01 00:00:00

abstract：:Nuclear FGFR1 acts as a developmental gene regulator in cooperation with FGF-2, RSK1, and CREB-binding protein (CBP). FRAP analysis revealed three nuclear FGFR1 populations: i) a fast mobile, ii) a slower mobile population reflecting chromatin-bound FGFR1, and iii) an immobile FGFR1 population associated with the nucl...

journal_title：Molecular biology of the cell

authors： Dunham-Ems SM,Lee YW,Stachowiak EK,Pudavar H,Claus P,Prasad PN,Stachowiak MK

更新日期：2009-05-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:The chromosomal passenger complex (CPC) regulates various events in cell division. This complex is composed of a catalytic subunit, Aurora B kinase, and three nonenzymatic subunits, INCENP, Survivin, and Borealin. Together, these four subunits interdependently regulate CPC function, and they are highly conserved among...

journal_title：Molecular biology of the cell

authors： Bohnert KA,Chen JS,Clifford DM,Vander Kooi CW,Gould KL

更新日期：2009-08-01 00:00:00

abstract：:The huntingtin exon 1 proteins with a polyglutamine repeat in the pathological range (51 or 83 glutamines), but not with a polyglutamine tract in the normal range (20 glutamines), form aggresome-like perinuclear inclusions in human 293 Tet-Off cells. These structures contain aggregated, ubiquitinated huntingtin exon 1...

journal_title：Molecular biology of the cell

authors： Waelter S,Boeddrich A,Lurz R,Scherzinger E,Lueder G,Lehrach H,Wanker EE

更新日期：2001-05-01 00:00:00

abstract：:The kinetochore is a crucial structure for faithful chromosome segregation during mitosis and is formed in the centromeric region of each chromosome. The 16-subunit protein complex known as the constitutive centromere-associated network (CCAN) forms the foundation for kinetochore assembly on the centromeric chromatin....

journal_title：Molecular biology of the cell

authors： Nagpal H,Hori T,Furukawa A,Sugase K,Osakabe A,Kurumizaka H,Fukagawa T

更新日期：2015-11-01 00:00:00

abstract：:When Candida albicans yeast cells receive the appropriate stimulus, they switch to hyphal growth, characterized by continuous apical elongation and the inhibition of cell separation. The molecular basis of this inhibition is poorly known, despite its crucial importance for hyphal development. In C. albicans, septins a...

journal_title：Molecular biology of the cell

authors： González-Novo A,Correa-Bordes J,Labrador L,Sánchez M,Vázquez de Aldana CR,Jiménez J

更新日期：2008-04-01 00:00:00

abstract：:When proliferating fission yeast cells are exposed to nitrogen starvation, they initiate conjugation and differentiate into ascospores. Cell cycle arrest in the G1-phase is one of the prerequisites for cell differentiation, because conjugation occurs only in the pre-Start G1-phase. The role of ste9(+) in the cell cycl...

journal_title：Molecular biology of the cell

authors： Kitamura K,Maekawa H,Shimoda C

更新日期：1998-05-01 00:00:00

abstract：:We used transgenic analysis in Drosophila to compare the ability of two structurally similar alpha-tubulin isoforms to support microtubule assembly in vivo. Our data revealed that even closely related alpha-tubulin isoforms have different functional capacities. Thus, in multicellular organisms, even small changes in t...

journal_title：Molecular biology of the cell

authors： Hutchens JA,Hoyle HD,Turner FR,Raff EC

更新日期：1997-03-01 00:00:00

abstract：:Lamellipodia of crawling cells represent both the motor for cell advance and the primary building site for the actin cytoskeleton. The organization of actin in the lamellipodium reflects actin dynamics and is of critical importance for the mechanism of cell motility. In previous structural studies, the lamellipodial a...

journal_title：Molecular biology of the cell

authors： Verkhovsky AB,Chaga OY,Schaub S,Svitkina TM,Meister JJ,Borisy GG

更新日期：2003-11-01 00:00:00

abstract：:"Lipid raft" is the name given to the tiny, dynamic, and ordered domains of cholesterol and sphingolipids that are hypothesized to exist in the plasma membranes of eukaryotic cells. According to the lipid raft hypothesis, these cholesterol- and sphingolipid-enriched domains modulate the protein-protein interactions th...

journal_title：Molecular biology of the cell

authors： Kraft ML

更新日期：2013-09-01 00:00:00

abstract：:Formins have conserved roles in cell polarity and cytokinesis and directly nucleate actin filament assembly through their FH2 domain. Here, we define the active region of the yeast formin Bni1 FH2 domain and show that it dimerizes. Mutations that disrupt dimerization abolish actin assembly activity, suggesting that di...

journal_title：Molecular biology of the cell

authors： Moseley JB,Sagot I,Manning AL,Xu Y,Eck MJ,Pellman D,Goode BL

更新日期：2004-02-01 00:00:00

abstract：:Actin and microtubule dynamics must be precisely coordinated during cell migration, mitosis, and morphogenesis--much of this coordination is mediated by proteins that physically bridge the two cytoskeletal networks. We have investigated the regulation of the Drosophila actin-microtubule cross-linker Short stop (Shot),...

journal_title：Molecular biology of the cell

authors： Applewhite DA,Grode KD,Duncan MC,Rogers SL

更新日期：2013-09-01 00:00:00

abstract：:Rab proteins are thought to function in the processes by which transport vesicles identify and/or fuse with their respective target membranes. The bulk of these proteins are membrane associated, but a measurable fraction can be found in the cytosol. The cytosolic forms of rab3A, rab11, and Sec4 occur as equimolar comp...

journal_title：Molecular biology of the cell

authors： Soldati T,Riederer MA,Pfeffer SR

更新日期：1993-04-01 00:00:00

abstract：:Claspin is essential for the ATR-dependent activation of Chk1 in Xenopus egg extracts containing incompletely replicated DNA. Claspin associates with replication forks upon origin unwinding. We show that Claspin contains a replication fork-interacting domain (RFID, residues 265-605) that associates with Cdc45, DNA pol...

journal_title：Molecular biology of the cell

authors： Lee J,Gold DA,Shevchenko A,Shevchenko A,Dunphy WG

更新日期：2005-11-01 00:00:00

abstract：:Nuclear migration is required for normal development in both higher and lower eukaryotes. In fungi this process is mediated by cytoplasmic dynein. It is believed that this motor protein is anchored to the cell membrane and moves nuclei by capturing and pulling on spindle pole body microtubules. To date, four genes hav...

journal_title：Molecular biology of the cell

authors： Chiu YH,Xiang X,Dawe AL,Morris NR

更新日期：1997-09-01 00:00:00

abstract：:Pro-alpha-factor (pro-alphaf) is posttranslationally modified in the yeast Golgi complex by the addition of alpha1,6-, alpha1,2-, and alpha1,3-linked mannose to N-linked oligosaccharides and by a Kex2p-initiated proteolytic processing event. Previous work has indicated that the alpha1,6- and alpha1,3-mannosylation and...

journal_title：Molecular biology of the cell

authors： Brigance WT,Barlowe C,Graham TR

更新日期：2000-01-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00

abstract：:The secretory and autophagy pathways can be thought of as the biosynthetic (i.e., anabolic) and degradative (i.e., catabolic) branches of the endomembrane system. In analogy to anabolic and catabolic pathways in metabolism, there is mounting evidence that the secretory and autophagy pathways are intimately linked and ...

journal_title：Molecular biology of the cell

authors： Farhan H,Kundu M,Ferro-Novick S

更新日期：2017-05-01 00:00:00

abstract：:Based on its shedding and binding activities, the disintegrin and metalloprotease 12 (ADAM12) has been implicated in cell signaling. Here we investigate the intracellular protein interaction network of the transmembrane ADAM12L variant using an integrative approach. We identify the integrin-linked kinase (ILK) as a ne...

journal_title：Molecular biology of the cell

authors： Leyme A,Bourd-Boittin K,Bonnier D,Falconer A,Arlot-Bonnemains Y,Théret N

更新日期：2012-09-01 00:00:00

abstract：:Previously, we showed that spliceosomal U6 small nuclear RNA (snRNA) transiently passes through the nucleolus. Herein, we report that all individual snRNAs of the [U4/U6.U5] tri-snRNP localize to nucleoli, demonstrated by fluorescence microscopy of nucleolar preparations after injection of fluorescein-labeled snRNA in...

journal_title：Molecular biology of the cell

authors： Gerbi SA,Lange TS

更新日期：2002-09-01 00:00:00

abstract：:Membrane fusion is generally controlled by Rabs, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), and tethering complexes. Syntaxin 17 (STX17) was recently identified as the autophagosomal SNARE required for autophagosome-lysosome fusion in mammals and Drosophila. In this study, to bett...

journal_title：Molecular biology of the cell

authors： Jiang P,Nishimura T,Sakamaki Y,Itakura E,Hatta T,Natsume T,Mizushima N

更新日期：2014-04-01 00:00:00