Abstract:
:Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Hu Z,Feng J,Bo W,Wu R,Dong Z,Liu Y,Qiang L,Liu Mdoi
10.1091/mbc.E16-09-0628subject
Has Abstractpub_date
2017-02-15 00:00:00pages
545-553issue
4eissn
1059-1524issn
1939-4586pii
mbc.E16-09-0628journal_volume
28pub_type
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