Abstract:
:During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway.
journal_name
Mol Biol Celljournal_title
Molecular biology of the cellauthors
Szatmári Z,Kis V,Lippai M,Hegedus K,Faragó T,Lorincz P,Tanaka T,Juhász G,Sass Mdoi
10.1091/mbc.E13-10-0574subject
Has Abstractpub_date
2014-02-01 00:00:00pages
522-31issue
4eissn
1059-1524issn
1939-4586pii
mbc.E13-10-0574journal_volume
25pub_type
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