Caveolin 2 regulates endocytosis and trafficking of the M1 muscarinic receptor in MDCK epithelial cells.

abstract：:Trafficking through the Golgi apparatus requires members of the Arf family of GTPases, whose activation is regulated by guanine nucleotide exchange factors (GEFs). Once activated, Arf-GTP recruits effectors such as coat complexes and lipid-modifying enzymes to specific membrane sites, creating a domain competent for c...

journal_title：Molecular biology of the cell

authors： Niu TK,Pfeifer AC,Lippincott-Schwartz J,Jackson CL

更新日期：2005-03-01 00:00:00

abstract：:Ca2+-triggered exocytosis of synaptic vesicles is controlled by the Ca2+-binding protein synaptotagmin (syt) I. Fifteen additional isoforms of syt have been identified. Here, we compared the abilities of three syt isoforms (I, VII, and IX) to regulate soluble N-ethylmaleimide-sensitive factor attachment protein recept...

journal_title：Molecular biology of the cell

authors： Bhalla A,Tucker WC,Chapman ER

更新日期：2005-10-01 00:00:00

abstract：:Myosin- and Rab-interacting protein (MyRIP), which belongs to the protein kinase A (PKA)-anchoring family, is implicated in hormone secretion. However, its mechanism of action is not fully elucidated. Here we investigate the role of MyRIP in myosin Va (MyoVa)-dependent secretory granule (SG) transport and secretion in...

journal_title：Molecular biology of the cell

authors： Brozzi F,Lajus S,Diraison F,Rajatileka S,Hayward K,Regazzi R,Molnár E,Váradi A

更新日期：2012-11-01 00:00:00

abstract：:Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outst...

journal_title：Molecular biology of the cell

authors： Graef M,Friedman JR,Graham C,Babu M,Nunnari J

更新日期：2013-09-01 00:00:00

abstract：:Sm-like (Lsm) proteins function in a variety of RNA-processing events. In yeast, the Lsm2-Lsm8 complex binds and stabilizes the spliceosomal U6 snRNA, whereas the Lsm1-Lsm7 complex functions in mRNA decay. Here we report that a third Lsm complex, consisting of Lsm2-Lsm7 proteins, associates with snR5, a box H/ACA snoR...

journal_title：Molecular biology of the cell

authors： Fernandez CF,Pannone BK,Chen X,Fuchs G,Wolin SL

更新日期：2004-06-01 00:00:00

abstract：:Meiotic recombination differs from mitotic recombination in that DSBs are repaired using homologous chromosomes, rather than sister chromatids. This change in partner choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-s...

journal_title：Molecular biology of the cell

authors： Niu H,Wan L,Baumgartner B,Schaefer D,Loidl J,Hollingsworth NM

更新日期：2005-12-01 00:00:00

abstract：:Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin l...

journal_title：Molecular biology of the cell

authors： Leverson JD,Joazeiro CA,Page AM,Huang Hk,Hieter P,Hunter T

更新日期：2000-07-01 00:00:00

abstract：:RhoA is a small guanosine-5'-triphosphatase (GTPase) suggested to be essential for cytokinesis, stress fiber formation, and epithelial cell-cell contacts. In skin, loss of RhoA was suggested to underlie pemphigus skin blistering. To analyze RhoA function in vivo, we generated mice with a keratinocyte-restricted deleti...

journal_title：Molecular biology of the cell

authors： Jackson B,Peyrollier K,Pedersen E,Basse A,Karlsson R,Wang Z,Lefever T,Ochsenbein AM,Schmidt G,Aktories K,Stanley A,Quondamatteo F,Ladwein M,Rottner K,van Hengel J,Brakebusch C

更新日期：2011-03-01 00:00:00

abstract：:During Aspergillus nidulans mitosis peripheral nuclear pore complex (NPC) proteins (Nups) disperse from the core NPC structure. Unexpectedly, one predicted peripheral Nup, Gle1, remains at the mitotic NE via an unknown mechanism. Gle1 affinity purification identified MtgA ( M: itotic T: ether for G: le1), which tether...

journal_title：Molecular biology of the cell

authors： Chemudupati M,Osmani AH,Osmani SA

更新日期：2016-09-14 00:00:00

abstract：:Cell adhesion to extracellular matrix molecules such as fibronectin involves complex transmembrane signaling processes. Attachment and spreading of primary fibroblasts can be promoted by interactions of cell surface integrins with RGD-containing fragments of fibronectin, but the further process of focal adhesion and s...

journal_title：Molecular biology of the cell

authors： Woods A,McCarthy JB,Furcht LT,Couchman JR

更新日期：1993-06-01 00:00:00

abstract：:Transforming growth factor (TGF)-beta receptors stimulate diverse signaling processes that control a wide range of biological responses. In polarized epithelia, the TGFbeta type II receptor (T2R) is localized at the basolateral membranes. Sequential cytoplasmic truncations resulted in receptor missorting to apical sur...

journal_title：Molecular biology of the cell

authors： Murphy SJ,Shapira KE,Henis YI,Leof EB

更新日期：2007-10-01 00:00:00

abstract：:We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon addition of the horm...

journal_title：Molecular biology of the cell

authors： McIsaac RS,Silverman SJ,McClean MN,Gibney PA,Macinskas J,Hickman MJ,Petti AA,Botstein D

更新日期：2011-11-01 00:00:00

abstract：:Soluble N-ethyl maleimide-sensitive fusion protein attachment protein receptors (SNAREs) are core machinery for membrane fusion during intracellular vesicular transport. Synaptosome-associated protein of 23 kDa (SNAP23) is a target SNARE previously identified at the plasma membrane, where it is involved in exocytotic ...

journal_title：Molecular biology of the cell

authors： Faigle W,Colucci-Guyon E,Louvard D,Amigorena S,Galli T

更新日期：2000-10-01 00:00:00

abstract：:During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recy...

journal_title：Molecular biology of the cell

authors： Szatmári Z,Kis V,Lippai M,Hegedus K,Faragó T,Lorincz P,Tanaka T,Juhász G,Sass M

更新日期：2014-02-01 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:Stimulation of the T-cell receptor (TCR) results in the activation of several transcription factors, including NF-kappaB, that are crucial for T-cell proliferation and gain of effector functions. On TCR engagement, several proteins within the TCR-directed NF-kappaB signaling pathway undergo dynamic spatial redistribut...

journal_title：Molecular biology of the cell

authors： Rossman JS,Stoicheva NG,Langel FD,Patterson GH,Lippincott-Schwartz J,Schaefer BC

更新日期：2006-05-01 00:00:00

abstract：:The Forkhead family of transcription factors participates in the induction of death-related genes. In NMuMG and 4T1 mammary epithelial cells, transforming growth factor beta (TGF beta) induced phosphorylation and cytoplasmic retention of the Forkhead factor FKHRL1, while reducing FHKRL1-dependent transcriptional activ...

journal_title：Molecular biology of the cell

authors： Shin I,Bakin AV,Rodeck U,Brunet A,Arteaga CL

更新日期：2001-11-01 00:00:00

abstract：:Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobo...

journal_title：Molecular biology of the cell

authors： Sao K,Jones TM,Doyle AD,Maity D,Schevzov G,Chen Y,Gunning PW,Petrie RJ

更新日期：2019-05-01 00:00:00

abstract：:Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central alpha5beta1 integrin-binding and the C-terminal heparin-binding regions of FN. We found that within the heparin-binding region, the i...

journal_title：Molecular biology of the cell

authors： Bloom L,Ingham KC,Hynes RO

更新日期：1999-05-01 00:00:00

abstract：:More than 97% of mice in which the C-terminal region of connexin43 (Cx43) was removed (designated as Cx43K258stop) die shortly after birth due to a defect of the epidermal barrier. The abnormal expression of Cx43K258stop protein in the uppermost layers of the epidermis seems to perturb terminal differentiation of kera...

journal_title：Molecular biology of the cell

authors： Maass K,Ghanem A,Kim JS,Saathoff M,Urschel S,Kirfel G,Grümmer R,Kretz M,Lewalter T,Tiemann K,Winterhager E,Herzog V,Willecke K

更新日期：2004-10-01 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is facilitated by a precisely regulated burst of actin assembly. PtdIns(4,5)P2 is an important signaling lipid with conserved roles in CME and actin assembly regulation. Rhomboid family multipass transmembrane proteins regulate diverse cellular processes; however, rhomboid-mediated ...

journal_title：Molecular biology of the cell

authors： Cortesio CL,Lewellyn EB,Drubin DG

更新日期：2015-04-15 00:00:00

abstract：:Pif1 family helicases, which are found in nearly all eukaryotes, have important roles in both nuclear and mitochondrial genome maintenance. Recently, the increasing availability of genome sequences has revealed that Pif1 helicases are also widely found in diverse prokaryotes, but it is currently unknown what physiolog...

journal_title：Molecular biology of the cell

authors： Bochman ML,Judge CP,Zakian VA

更新日期：2011-06-15 00:00:00

abstract：:The homotypic fusion of yeast vacuoles requires the Rab-family GTPase Ypt7p and its effector complex, homotypic fusion and vacuole protein sorting complex (HOPS). Although the vacuolar kinase Yck3p is required for the sensitivity of vacuole fusion to proteins that regulate the Rab GTPase cycle-Gdi1p (GDP-dissociation ...

journal_title：Molecular biology of the cell

authors： Zick M,Wickner W

更新日期：2012-09-01 00:00:00

abstract：:Fission yeast rad22(+), a homologue of budding yeast RAD52, encodes a double-strand break repair component, which is dispensable for proliferation. We, however, have recently obtained a cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation...

journal_title：Molecular biology of the cell

authors： Suto K,Nagata A,Murakami H,Okayama H

更新日期：1999-10-01 00:00:00

abstract：:An inducible fluorescent system based on GFP is presented that allows for the uncoupling of dendritic mRNA transport from subsequent protein synthesis at the single cell level. The iron-responsive element (IRE) derived from ferritin mRNA in the 5'-UTR of the GFP reporter mRNA renders translation of its mRNA dependent ...

journal_title：Molecular biology of the cell

authors： Macchi P,Hemraj I,Goetze B,Grunewald B,Mallardo M,Kiebler MA

更新日期：2003-04-01 00:00:00

abstract：:Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the sali...

journal_title：Molecular biology of the cell

authors： Shitara A,Malec L,Ebrahim S,Chen D,Bleck C,Hoffman MP,Weigert R

更新日期：2019-02-01 00:00:00

abstract：:Microtubules (MTs) often form a polarized array with minus-ends anchored at the centrosome and plus-ends extended towards the cell margins. Plus-ends display behavior known as dynamic instability, - transitions between rapid shortening and slow growth. It is known that dynamic instability is regulated locally to ensur...

journal_title：Molecular biology of the cell

authors： Burakov A,Vorobjev I,Semenova I,Cowan A,Carson J,Wu Y,Rodionov V

更新日期：2021-01-13 00:00:00

abstract：:RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct RIO kinases-hRio1, hRio2, and hRio3, of which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 and hRio3, like hRio2, are associated with precursors of 40S rib...

journal_title：Molecular biology of the cell

authors： Widmann B,Wandrey F,Badertscher L,Wyler E,Pfannstiel J,Zemp I,Kutay U

更新日期：2012-01-01 00:00:00

abstract：:Proteolytic processing of the amyloid-β precursor protein (APP) and generation of amyloid-β peptide (Aβ) are key events in Alzheimer's disease (AD) pathogenesis. Cell biological and genetic evidence has implicated the low-density lipoprotein and sorting receptor LR11/SorLA in AD through mechanisms related to APP and A...

journal_title：Molecular biology of the cell

authors： Herskowitz JH,Offe K,Deshpande A,Kahn RA,Levey AI,Lah JJ

更新日期：2012-07-01 00:00:00

abstract：:The microphthalmia-associated transcription factor (MITF) is required for terminal osteoclast differentiation and is a target for signaling pathways engaged by colony stimulating factor (CSF)-1 and receptor-activator of nuclear factor-kappaB ligand (RANKL). Work presented here demonstrates that MITF can shuttle from c...

journal_title：Molecular biology of the cell

authors： Bronisz A,Sharma SM,Hu R,Godlewski J,Tzivion G,Mansky KC,Ostrowski MC

更新日期：2006-09-01 00:00:00